FastQCFastQC Report
Fri 27 May 2016
SRR939688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020951
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51990.5092310992398265No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40310.3948279594221466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.3526124172462733No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31700.3104948229640796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.2706300302365148No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23420.2293939669974367No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.2072577430258651No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20400.19981370310622154No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15230.1491746420739095No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14500.14202444583530455No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT13900.1361475722145333No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13880.13595167642717426No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12270.1201820655447715No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC12050.11802721188382205No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG10890.10666525621699768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATG4350.040.3002361
GTACATG61500.035.7151451
CATTCCG1850.033.1647769
CATATAG2300.028.7505763
TACATGG75750.028.4957392
ACATGGG75000.027.710083
ATGGGAT19750.027.001975
CGCGTCA350.008631065526.9876926-27
CATGGGA58300.026.0850124
ATATGAG4600.023.6165453
GAGTACT74850.023.45729312-13
CATGGGG13900.023.4443444
AGTACTT78600.023.20885312-13
TAGGACA4950.022.8986454
ATGGGAG20150.022.7185615
CCTTTAG3202.6456282E-522.5577621
GCCGTAT1306.657501E-1021.81648694-95
TAGGCAT3251.8189894E-1221.7817155
TTTGCGT2658.5128704E-1021.388573
ATAGCCC3101.2732926E-1121.3310743
Produced by FastQC (version 0.11.5)