##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939688_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1020951 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.084395823110022 2.0 2.0 31.0 2.0 33.0 2 22.55647332731933 16.0 16.0 31.0 16.0 34.0 3 27.220059532729778 28.0 25.0 31.0 16.0 34.0 4 32.52808900720994 35.0 32.0 35.0 27.0 37.0 5 33.16803157056509 35.0 33.0 37.0 28.0 37.0 6 33.64462839058877 35.0 33.0 37.0 28.0 37.0 7 33.84844130619393 35.0 35.0 37.0 29.0 37.0 8 33.81247483963481 35.0 35.0 37.0 28.0 37.0 9 35.14064142157655 37.0 35.0 39.0 28.0 39.0 10-11 35.003250890591225 37.0 34.0 39.0 27.5 39.0 12-13 35.026212815306515 37.0 34.0 39.0 27.0 39.0 14-15 35.589428875626744 38.0 34.0 40.0 27.0 41.0 16-17 35.287026017898995 38.0 34.0 40.0 27.0 41.0 18-19 35.68778668124131 38.0 34.0 40.0 27.0 41.0 20-21 35.73419586248508 38.0 34.0 40.0 27.0 41.0 22-23 35.5669498340273 38.0 34.0 40.0 27.0 41.0 24-25 35.3201755030359 38.0 34.0 40.0 26.5 41.0 26-27 35.291195659732935 38.0 34.0 40.0 26.0 41.0 28-29 35.02086486031161 38.0 34.0 40.0 25.0 41.0 30-31 34.80370017757953 38.0 34.0 40.0 25.0 41.0 32-33 34.387783546908715 38.0 33.0 40.0 24.0 41.0 34-35 34.06656440906566 38.0 33.0 40.0 23.5 41.0 36-37 33.66879507439633 37.0 33.0 40.0 21.0 41.0 38-39 33.37919498585143 37.0 32.5 40.0 19.5 41.0 40-41 32.945578191313786 37.0 31.5 40.0 16.5 41.0 42-43 32.63309257741067 37.0 31.0 40.0 15.0 41.0 44-45 32.169008600804545 36.5 30.5 40.0 11.0 41.0 46-47 31.63079618904335 36.0 30.0 39.0 8.0 41.0 48-49 31.11377725277707 36.0 29.5 39.0 3.5 40.0 50-51 30.40315842777959 35.5 28.5 38.5 2.0 40.0 52-53 30.399844360796944 35.0 28.0 38.5 2.0 40.0 54-55 30.546885697746514 35.5 28.0 39.0 2.0 40.0 56-57 30.29615770002674 35.0 28.0 39.0 2.0 40.0 58-59 29.946726630367177 35.0 28.0 39.0 2.0 40.0 60-61 29.354766291428284 35.0 26.0 38.0 2.0 40.0 62-63 28.93425247636762 34.0 26.0 38.0 2.0 40.0 64-65 28.3313626217125 34.0 25.5 37.5 2.0 39.5 66-67 27.755878097969443 33.5 24.0 37.0 2.0 39.0 68-69 27.45045893485584 33.0 23.5 36.5 2.0 39.0 70-71 26.9277580412772 33.0 22.5 36.0 2.0 39.0 72-73 26.33124655345849 32.0 21.5 35.5 2.0 38.0 74-75 25.76025636881692 32.0 20.0 35.0 2.0 37.5 76-77 25.106443893977282 31.0 17.5 35.0 2.0 37.0 78-79 24.461689640345128 31.0 12.5 34.5 2.0 36.0 80-81 23.746020621949533 30.5 7.0 34.0 2.0 36.0 82-83 23.086668703982852 30.0 3.5 34.0 2.0 35.0 84-85 22.432749955678577 29.0 2.0 34.0 2.0 35.0 86-87 21.736277255225765 29.0 2.0 34.0 2.0 35.0 88-89 20.89324463172082 28.0 2.0 33.0 2.0 35.0 90-91 19.983522715585764 26.5 2.0 33.0 2.0 34.0 92-93 19.050045986536084 25.5 2.0 32.0 2.0 34.0 94-95 18.111532287053933 24.5 2.0 32.0 2.0 34.0 96-97 17.17352057052689 21.5 2.0 32.0 2.0 34.0 98-99 16.127711809871386 10.0 2.0 31.0 2.0 34.0 100-101 14.525386624823327 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.758231372041521 1101 2 7.936587467323896 1101 3 3.7009428954610186 1101 4 2.30429642413543 1101 5 1.4041137754059108 1101 6 1.1542376483613026 1101 7 0.8970166436738225 1101 8 0.9594872415213871 1101 9 0.8437827773866573 1101 10-11 0.8963428333381174 1101 12-13 0.4722681381697953 1101 14-15 0.581969695720943 1101 16-17 0.7709515960383939 1101 18-19 0.9309563525889075 1101 20-21 1.197873848508209 1101 22-23 0.9670154480724094 1101 24-25 1.1109614983899263 1101 26-27 1.0909085581098168 1101 28-29 1.1778915596681685 1101 30-31 1.3251654676647888 1101 32-33 0.9573625867332609 1101 34-35 0.9811793717134307 1101 36-37 1.0330463733809623 1101 38-39 0.7656654618778376 1101 40-41 1.3194389460599965 1101 42-43 1.2224060066983853 1101 44-45 0.9209026473328024 1101 46-47 0.3768363669002781 1101 48-49 0.010032946164162837 1101 50-51 0.10301763014021503 1101 52-53 0.44128716459965034 1101 54-55 0.38868362746618956 1101 56-57 0.45426249604910396 1101 58-59 0.33399214982031467 1101 60-61 0.1619936630416028 1101 62-63 -0.1668873396467596 1101 64-65 -0.09384453430619644 1101 66-67 -0.38915804994511305 1101 68-69 -0.3991884363733895 1101 70-71 -0.7454868807181505 1101 72-73 -0.5612138090240748 1101 74-75 -0.6375829341950876 1101 76-77 -0.7160021934022538 1101 78-79 -0.8264607127393866 1101 80-81 -0.5817932534157144 1101 82-83 -0.47169250021903153 1101 84-85 -0.5609642464133984 1101 86-87 -0.6279293403661335 1101 88-89 -0.6808258279778343 1101 90-91 -0.99533052637004 1101 92-93 -0.464619674207448 1101 94-95 -0.5808083614336219 1101 96-97 -1.0437503877558107 1101 98-99 -1.2140513858890074 1101 100-101 -1.3922802155714606 1102 1 14.791230309843954 1102 2 7.836461577241671 1102 3 3.680985383363769 1102 4 2.1274995399149645 1102 5 1.2602882905323867 1102 6 1.1294648406190575 1102 7 0.9081500478203282 1102 8 0.8073490771561467 1102 9 0.9079906533849211 1102 10-11 0.9884156922231995 1102 12-13 0.7083081082708986 1102 14-15 0.8985832525091837 1102 16-17 0.9485943042487861 1102 18-19 0.9862024283589932 1102 20-21 0.8901827512974592 1102 22-23 0.7151644704572391 1102 24-25 0.6149683436631506 1102 26-27 0.7807330988077297 1102 28-29 0.9010514007319443 1102 30-31 0.8944994304485334 1102 32-33 1.0266926154519282 1102 34-35 0.8161453420505751 1102 36-37 0.8774875394378441 1102 38-39 0.8828002822877679 1102 40-41 0.8491017966835415 1102 42-43 1.0150375028233256 1102 44-45 1.0189080763425906 1102 46-47 0.7573745470017812 1102 48-49 0.8140751980230085 1102 50-51 0.94707719188537 1102 52-53 1.0664750162067165 1102 54-55 1.0299637722397819 1102 56-57 1.0209488330411176 1102 58-59 0.7000136298155937 1102 60-61 0.9613091357195174 1102 62-63 0.5699119050127486 1102 64-65 0.7768584831217105 1102 66-67 0.7394603064024992 1102 68-69 0.5521275083923349 1102 70-71 0.5713312474536885 1102 72-73 0.7918803331530384 1102 74-75 0.4437046853021407 1102 76-77 0.38464889436673744 1102 78-79 0.2845999112033333 1102 80-81 0.12393081126031902 1102 82-83 0.3207954025308801 1102 84-85 -0.1448483488564527 1102 86-87 0.06215091456128263 1102 88-89 0.03264244378737757 1102 90-91 0.11781908612454828 1102 92-93 -0.08059394542189224 1102 94-95 -0.2381178543326392 1102 96-97 -0.5656001851514567 1102 98-99 -0.7382911278143407 1102 100-101 -0.9761466147217011 1103 1 14.935061947109983 1103 2 8.005282153199197 1103 3 3.877499628405417 1103 4 2.242098543435347 1103 5 1.7063918243799918 1103 6 1.1615599486351442 1103 7 0.8061089959379828 1103 8 0.9005834037629228 1103 9 1.096594620066341 1103 10-11 0.8715621054736857 1103 12-13 0.6971679278272944 1103 14-15 0.9760681995110758 1103 16-17 1.076741967908056 1103 18-19 1.04068370108277 1103 20-21 0.8555791308830862 1103 22-23 1.100451968293065 1103 24-25 1.1217782622903911 1103 26-27 0.9492151025397106 1103 28-29 0.8619809353874999 1103 30-31 0.8339681028991492 1103 32-33 0.6883146842051744 1103 34-35 0.5925299293194897 1103 36-37 0.7696638278398282 1103 38-39 0.6928016288818455 1103 40-41 0.7983967584979794 1103 42-43 0.6576879847116359 1103 44-45 0.5436315436299779 1103 46-47 0.3550718711269738 1103 48-49 0.32238560719524045 1103 50-51 0.11236199653304269 1103 52-53 0.5650380079540156 1103 54-55 0.47481689729989895 1103 56-57 0.6687721598979763 1103 58-59 0.5296463943732377 1103 60-61 0.6741498721141106 1103 62-63 0.38008253771959133 1103 64-65 0.08800251097697398 1103 66-67 0.2215833081530718 1103 68-69 0.2294816433980067 1103 70-71 0.11710390389906422 1103 72-73 0.01713281954281598 1103 74-75 0.02470424284980055 1103 76-77 0.14116929526703004 1103 78-79 -0.1930658641463019 1103 80-81 -0.11498768132379666 1103 82-83 -0.1513031681556889 1103 84-85 -0.48390150084844663 1103 86-87 -0.2225537004089979 1103 88-89 -0.4077476837543159 1103 90-91 -0.4096437382603426 1103 92-93 -0.16857847882704746 1103 94-95 -0.17138065178565043 1103 96-97 -0.6036106885852739 1103 98-99 -1.0793468575721459 1103 100-101 -0.9818504024984751 1104 1 14.814135504350151 1104 2 7.9974523944809555 1104 3 3.661579677446113 1104 4 2.10616519004914 1104 5 1.3178569913963116 1104 6 1.055036545789413 1104 7 0.7829814330093967 1104 8 0.6628502415135955 1104 9 0.6138864160296222 1104 10-11 0.41240409771398134 1104 12-13 0.37863907263061236 1104 14-15 0.4638084056495444 1104 16-17 0.6866780398547263 1104 18-19 0.3135114227844227 1104 20-21 0.5089641955536095 1104 22-23 0.643460432382561 1104 24-25 0.784652778260984 1104 26-27 0.5832633588915854 1104 28-29 0.5293262268331773 1104 30-31 0.3359373458524004 1104 32-33 0.46677104445632267 1104 34-35 0.291481722465214 1104 36-37 0.4874753485964547 1104 38-39 0.4118220333153104 1104 40-41 0.4699836134585951 1104 42-43 0.21573762699629384 1104 44-45 0.16154129808381157 1104 46-47 0.0026954152312406165 1104 48-49 -0.12742763455484507 1104 50-51 -0.2653842635425754 1104 52-53 -0.36294831547852624 1104 54-55 -0.11358848042389624 1104 56-57 -0.19507136676790537 1104 58-59 -0.2843941708131972 1104 60-61 -0.25881971732234277 1104 62-63 -0.589597517413118 1104 64-65 -0.7541511676465369 1104 66-67 -0.6955021208959238 1104 68-69 -0.336685224431978 1104 70-71 -0.24884365149065246 1104 72-73 -0.5265240630983747 1104 74-75 -0.6879473435482595 1104 76-77 -0.8003820356472673 1104 78-79 -0.9161945693271782 1104 80-81 -0.6660372208057481 1104 82-83 -0.9133461244569254 1104 84-85 -0.7689707403749466 1104 86-87 -0.8831665568393099 1104 88-89 -0.9497159367555881 1104 90-91 -0.8706818932944387 1104 92-93 -0.7209267591662183 1104 94-95 -0.6091602656282227 1104 96-97 -1.2465247264852053 1104 98-99 -1.2773422047978027 1104 100-101 -1.0849032621270904 1105 1 15.098360947054818 1105 2 8.093751966348858 1105 3 3.951573848417553 1105 4 2.332140547328386 1105 5 1.5647465011549144 1105 6 1.2347917265897976 1105 7 1.0441922629424667 1105 8 1.117202917841709 1105 9 1.0787212937205197 1105 10-11 1.0586323142322485 1105 12-13 0.8068867460442135 1105 14-15 1.0879415889021402 1105 16-17 1.3667146220339745 1105 18-19 1.0777121423679432 1105 20-21 1.0155469778092368 1105 22-23 1.0013125358578705 1105 24-25 1.064225048165106 1105 26-27 1.0269881081428593 1105 28-29 1.511577839866078 1105 30-31 1.3146262164278681 1105 32-33 0.8424268101695773 1105 34-35 0.989965170034111 1105 36-37 1.0954430276380762 1105 38-39 1.2282377837524834 1105 40-41 1.2492041316391393 1105 42-43 1.185120151970672 1105 44-45 1.2229018737506294 1105 46-47 1.2412685092394042 1105 48-49 1.047051270391787 1105 50-51 0.8976275352152356 1105 52-53 1.0746559537823899 1105 54-55 1.0004042031402527 1105 56-57 0.9501568698504528 1105 58-59 1.059056212093676 1105 60-61 0.8578211542179268 1105 62-63 0.8964932144314695 1105 64-65 0.9495784056283725 1105 66-67 1.134420895726855 1105 68-69 1.0591058149441146 1105 70-71 0.9741669412612524 1105 72-73 0.9556873497375449 1105 74-75 1.0297718982760244 1105 76-77 0.8703884011278724 1105 78-79 0.5818657711684914 1105 80-81 0.6158666605232703 1105 82-83 0.914627182612282 1105 84-85 0.8107613253121748 1105 86-87 0.8076989534016938 1105 88-89 0.49089329722966113 1105 90-91 0.9415580616813379 1105 92-93 1.2112856586746297 1105 94-95 1.0762078676756275 1105 96-97 0.7254255860713279 1105 98-99 0.42922650738888457 1105 100-101 0.5617520814840926 1106 1 14.782870645461283 1106 2 7.855723676704006 1106 3 3.3944144157618084 1106 4 2.0350564524564305 1106 5 1.225278842912438 1106 6 0.8349988974152325 1106 7 0.68149868669245 1106 8 0.6898419865102738 1106 9 0.6668424223975222 1106 10-11 0.49194819479713914 1106 12-13 0.4014900430014521 1106 14-15 0.4841230686060314 1106 16-17 0.5991822657324732 1106 18-19 0.3781425631897477 1106 20-21 0.39068373156199243 1106 22-23 0.5797037461265617 1106 24-25 0.5652584782966699 1106 26-27 0.3237889995268475 1106 28-29 0.5254317828181456 1106 30-31 0.3545070043233949 1106 32-33 0.28132313264502073 1106 34-35 0.6272986617351606 1106 36-37 0.5083424011160105 1106 38-39 0.38400697412178886 1106 40-41 0.7543494663363717 1106 42-43 0.17109127733487384 1106 44-45 0.22968704148304653 1106 46-47 0.25626213413092813 1106 48-49 0.629199673867479 1106 50-51 0.5542798679878231 1106 52-53 0.2850858413251558 1106 54-55 0.39216027484527416 1106 56-57 0.32488922033436296 1106 58-59 0.08141029967748636 1106 60-61 0.1227202432803729 1106 62-63 0.06825511988594357 1106 64-65 0.1267809692461377 1106 66-67 0.2532782888326892 1106 68-69 0.16245125209556122 1106 70-71 0.11001007965685616 1106 72-73 -0.021205508040164744 1106 74-75 -0.1973512390853429 1106 76-77 -0.17029917330899025 1106 78-79 -0.20191543575508675 1106 80-81 -0.23456553789531398 1106 82-83 -0.42722730331481173 1106 84-85 -0.29230656434108226 1106 86-87 -0.3140288545144294 1106 88-89 -0.4202054515038043 1106 90-91 -0.14690283038136798 1106 92-93 -0.0649456098992367 1106 94-95 -0.2846963958094655 1106 96-97 -0.579637494374623 1106 98-99 -0.7953583025617785 1106 100-101 -0.376508346141053 1107 1 14.870546316258306 1107 2 7.981285890549927 1107 3 3.625610683318989 1107 4 1.9462795115172895 1107 5 1.1394565678039186 1107 6 0.944864646963758 1107 7 0.7576446687190241 1107 8 0.9499681694256239 1107 9 0.7111687435456275 1107 10-11 0.8623407330783479 1107 12-13 0.5055406745098168 1107 14-15 0.678318717836845 1107 16-17 0.9513804911912089 1107 18-19 0.7907024264454066 1107 20-21 0.7807962099581687 1107 22-23 0.7614576553980541 1107 24-25 0.9715780746506795 1107 26-27 0.8521536331585793 1107 28-29 1.1564081130313468 1107 30-31 0.9014396384174859 1107 32-33 0.8117727374624266 1107 34-35 0.5888697965817684 1107 36-37 0.6923412072156694 1107 38-39 0.3916877593725232 1107 40-41 0.9028966368796532 1107 42-43 0.7786337506561907 1107 44-45 0.5397884042553684 1107 46-47 0.5242935523942762 1107 48-49 0.633332801672605 1107 50-51 0.5749819257241455 1107 52-53 0.5366306676118633 1107 54-55 0.7800630875573056 1107 56-57 0.7425192014837236 1107 58-59 0.7623740812697299 1107 60-61 0.8383728467827467 1107 62-63 0.7977975029521929 1107 64-65 0.6861129867556208 1107 66-67 0.7163085472392758 1107 68-69 0.7086733254760986 1107 70-71 0.8246009985721798 1107 72-73 0.9645048982897251 1107 74-75 0.8900445618373176 1107 76-77 0.976989176582979 1107 78-79 0.9694726000650924 1107 80-81 0.7618195092154529 1107 82-83 1.0289174436424453 1107 84-85 1.179314551227364 1107 86-87 0.964205344713335 1107 88-89 1.1644241108303817 1107 90-91 1.4970757712509837 1107 92-93 1.5201554163958626 1107 94-95 1.1033358616676558 1107 96-97 0.7945604033157174 1107 98-99 0.47823884474478184 1107 100-101 0.5200063040770235 1108 1 14.51256015782122 1108 2 7.781407874177315 1108 3 3.563499544203591 1108 4 -0.25029535269787573 1108 5 0.40433997902047025 1108 6 0.7071739904375249 1108 7 0.4772985763449853 1108 8 0.4997100012054787 1108 9 0.8275047269643849 1108 10-11 0.6359198560602337 1108 12-13 0.5291477806271985 1108 14-15 0.5699409858642639 1108 16-17 0.8367189272270963 1108 18-19 0.7147847509264267 1108 20-21 0.5899786379196854 1108 22-23 -0.08933664685483933 1108 24-25 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1.4790548513819708 2311 94-95 1.197422422541841 2311 96-97 1.0368898269269593 2311 98-99 1.3440873911859388 2311 100-101 1.2735737234739677 2312 1 -14.333660519850369 2312 2 -7.325198269461842 2312 3 -3.526003806485683 2312 4 -1.4889279190889155 2312 5 -1.8039313326391984 2312 6 -0.5192739751503197 2312 7 -0.1566940600368767 2312 8 0.10427398630812945 2312 9 -0.14174138313750717 2312 10-11 -0.024663750168471665 2312 12-13 -0.5466870057853512 2312 14-15 -0.05679739304613918 2312 16-17 -0.3601515350647375 2312 18-19 -0.09357587194331529 2312 20-21 -0.05110003796568208 2312 22-23 -4.56044399079758E-4 2312 24-25 0.2652943277227564 2312 26-27 0.23451168921295107 2312 28-29 -0.7410242092475983 2312 30-31 -0.17085922835990175 2312 32-33 0.13590949028016297 2312 34-35 -0.7827011171670648 2312 36-37 -0.1897659494313615 2312 38-39 -0.029100807888433167 2312 40-41 -0.0863483972273471 2312 42-43 -0.27937610758372955 2312 44-45 -0.18939077546062322 2312 46-47 -0.12078223071831573 2312 48-49 0.05659550522672063 2312 50-51 0.0627763398989245 2312 52-53 -0.2189385331260496 2312 54-55 -0.15046382418126214 2312 56-57 -0.0351456183590102 2312 58-59 -0.09136458803642356 2312 60-61 0.17836107940901869 2312 62-63 -0.2513255365849538 2312 64-65 -0.36683483229649383 2312 66-67 -0.23429759508465509 2312 68-69 -0.32546891265387146 2312 70-71 -0.2236904339217034 2312 72-73 -0.29892722173748965 2312 74-75 -0.24450034111249508 2312 76-77 -0.013108915508976082 2312 78-79 0.17553953926086407 2312 80-81 0.03243846081599955 2312 82-83 -0.10589444692097771 2312 84-85 0.06644314349399139 2312 86-87 0.18206876963197516 2312 88-89 0.7142121295601207 2312 90-91 0.7189405629010501 2312 92-93 1.3253184282306556 2312 94-95 1.242891474766214 2312 96-97 1.2055849615795395 2312 98-99 1.1359572766196546 2312 100-101 1.1850444867532435 2313 1 -14.333660519850369 2313 2 -7.510641822356549 2313 3 -3.8333281428952795 2313 4 -1.083572617197202 2313 5 -0.2653580054335478 2313 6 -1.2319800080162153 2313 7 -0.20681578555824842 2313 8 -0.2770560513274134 2313 9 -2.3610817674423643 2313 10-11 -0.46078036446004234 2313 12-13 -0.7771037914863257 2313 14-15 -0.9061713760790937 2313 16-17 -0.7919914535831651 2313 18-19 -0.10665826024774105 2313 20-21 -1.2383454080485308 2313 22-23 -2.2757125374619704 2313 24-25 -1.474298450724369 2313 26-27 -0.2940169535474837 2313 28-29 0.08608207700611104 2313 30-31 -1.0829746440277148 2313 32-33 -0.43473012765075225 2313 34-35 -1.338851671763443 2313 36-37 -1.2529048531564158 2313 38-39 -0.6767752914865426 2313 40-41 -0.4572440424347164 2313 42-43 -0.42845648990123664 2313 44-45 -0.5948431161927168 2313 46-47 -1.208536394849034 2313 48-49 -0.9262255147834644 2313 50-51 -1.4398351693733282 2313 52-53 -0.586055097733734 2313 54-55 -1.6447913217281247 2313 56-57 -0.86694547924413 2313 58-59 -0.5152589663046925 2313 60-61 -2.222224569400435 2313 62-63 -0.6351653867880387 2313 64-65 -0.5116121242762723 2313 66-67 -1.756633607207526 2313 68-69 -0.5939078449471538 2313 70-71 -0.5317693443540783 2313 72-73 -1.1915060017496621 2313 74-75 -0.7639546878314931 2313 76-77 -0.34285000035663415 2313 78-79 -0.44145359194186184 2313 80-81 -0.1897261418992251 2313 82-83 -0.2782548352005456 2313 84-85 -0.0714630222762942 2313 86-87 -0.2264507747667892 2313 88-89 0.15884021461893383 2313 90-91 0.9157555152666248 2313 92-93 1.5805173811427515 2313 94-95 1.832236299568411 2313 96-97 1.6373130913538496 2313 98-99 1.51451821390617 2313 100-101 1.4342883601090168 2314 1 -14.333660519850369 2314 2 -7.3702095082230805 2314 3 -3.595702857434734 2314 4 -1.3428802167912117 2314 5 -0.9554985154563838 2314 6 -5.743687062063408 2314 7 -1.9003691349619558 2314 8 -4.365818421284278 2314 9 -1.570880993527119 2314 10-11 -0.5272736402783593 2314 12-13 -2.9558528399511914 2314 14-15 -2.978993946492693 2314 16-17 -2.7367905210787242 2314 18-19 -1.3594755972180508 2314 20-21 -2.2479313316719782 2314 22-23 -1.1734486767667178 2314 24-25 -2.7829574205289873 2314 26-27 -4.495545753344491 2314 28-29 -1.9836472112455965 2314 30-31 -1.7588556069812853 2314 32-33 -4.299108616612948 2314 34-35 -2.8655588843998316 2314 36-37 -0.9255926227580389 2314 38-39 -1.5344954132830395 2314 40-41 -2.4900727978267483 2314 42-43 -1.742756477214101 2314 44-45 -3.2090804607753114 2314 46-47 -2.4772538840649716 2314 48-49 -1.3675491001678814 2314 50-51 -2.2922124213398334 2314 52-53 -0.9954245471120657 2314 54-55 -0.5577409220833651 2314 56-57 -1.3547666223550152 2314 58-59 -1.6798230046198412 2314 60-61 -0.736293016495079 2314 62-63 -3.10499062150004 2314 64-65 -1.3424529641646252 2314 66-67 -2.642214678001743 2314 68-69 -2.3628346718946283 2314 70-71 -2.9260974019536725 2314 72-73 -3.0796340149442827 2314 74-75 -2.207334382071533 2314 76-77 -1.119702322102384 2314 78-79 -0.508370300898978 2314 80-81 -0.6664095661570251 2314 82-83 -0.7946775388290668 2314 84-85 -0.8444584549076062 2314 86-87 -0.5698574291692218 2314 88-89 -0.1317387945158046 2314 90-91 0.1861889395244276 2314 92-93 0.7168113103485361 2314 94-95 0.799709656584394 2314 96-97 0.9008240974436745 2314 98-99 0.4581413425537182 2314 100-101 0.6176618693706235 2315 1 -14.333660519850369 2315 2 -7.511839075562483 2315 3 -4.9915254731846375 2315 4 -3.086689575714839 2315 5 -3.6722781289638355 2315 6 -2.8933853208683153 2315 7 -3.526995734355175 2315 8 -3.4176832808014943 2315 9 -2.291819029406284 2315 10-11 -4.938360568124288 2315 12-13 -4.657613821352058 2315 14-15 -3.658423606168199 2315 16-17 -4.628713997533019 2315 18-19 -1.656470963924285 2315 20-21 -3.424965611450368 2315 22-23 -4.385049636402648 2315 24-25 -5.460450495321233 2315 26-27 -5.0552707236650285 2315 28-29 -2.896399074456255 2315 30-31 -3.48939618540102 2315 32-33 -4.914642104939027 2315 34-35 -4.606737076590832 2315 36-37 -5.904742800834807 2315 38-39 -4.26661848411802 2315 40-41 -5.512496867870279 2315 42-43 -4.215958396485966 2315 44-45 -2.6798185725019934 2315 46-47 -2.0200067365910215 2315 48-49 -1.630541284576271 2315 50-51 -1.1518986434741194 2315 52-53 -1.1729637899560146 2315 54-55 -2.708603687549658 2315 56-57 -4.9976728187670965 2315 58-59 -4.088420567606253 2315 60-61 -4.637896531862445 2315 62-63 -3.00924715098164 2315 64-65 -3.5812943109526465 2315 66-67 -2.2417958678183894 2315 68-69 -1.8875391139207487 2315 70-71 -2.5812983154215523 2315 72-73 -2.8625963550049605 2315 74-75 -3.303300036338779 2315 76-77 -2.9719216863023554 2315 78-79 -2.2019504684230533 2315 80-81 -2.185274574071549 2315 82-83 -1.7034498808683765 2315 84-85 -1.2662860502116544 2315 86-87 -1.9211975393130025 2315 88-89 -2.353438191033412 2315 90-91 -2.052223291711556 2315 92-93 -0.8464731343157545 2315 94-95 -0.796262180904062 2315 96-97 -2.0936518127264865 2315 98-99 -1.5400539812821847 2315 100-101 -1.1608534979951575 2316 1 -14.333660519850369 2316 2 -7.4243041081702685 2316 3 -4.968852778216355 2316 4 -2.830134309740778 2316 5 -2.082998845532959 2316 6 -1.133847479280206 2316 7 -4.057140655954825 2316 8 -2.7231758282026846 2316 9 -1.0987444078392343 2316 10-11 -4.548240104498056 2316 12-13 -3.628113204355728 2316 14-15 -1.651041411504579 2316 16-17 -3.1109529517626413 2316 18-19 -2.0681244287469553 2316 20-21 -3.3613266643891677 2316 22-23 -2.94860793871549 2316 24-25 -3.353798455649141 2316 26-27 -3.941789481889515 2316 28-29 -3.3093676033765753 2316 30-31 -2.498318737427546 2316 32-33 -2.9871903765812604 2316 34-35 -3.9602520782207975 2316 36-37 -2.24875409621988 2316 38-39 -3.3071584711124515 2316 40-41 -3.021754372952177 2316 42-43 -7.10508978854233 2316 44-45 -3.721401394231872 2316 46-47 -4.6673161034998785 2316 48-49 -4.233592472230203 2316 50-51 -3.3891806679730543 2316 52-53 -4.830775748590806 2316 54-55 -4.933324332434562 2316 56-57 -4.929919282772232 2316 58-59 -3.535393872061089 2316 60-61 -3.408961025473161 2316 62-63 -2.6045103600793915 2316 64-65 -2.25850623852428 2316 66-67 -2.658117767518508 2316 68-69 -3.028398607727741 2316 70-71 -3.111480785898749 2316 72-73 -3.741097574490098 2316 74-75 -4.746972453104014 2316 76-77 -4.221359306522768 2316 78-79 -4.42722922616964 2316 80-81 -4.517970003178416 2316 82-83 -4.005813497371452 2316 84-85 -3.7440161522933977 2316 86-87 -3.8235056005449835 2316 88-89 -3.7611741985295346 2316 90-91 -2.920048682603909 2316 92-93 -2.7937024538447552 2316 94-95 -2.232291267630023 2316 96-97 -2.4298738494800105 2316 98-99 -2.100739367771075 2316 100-101 -1.8002084765496296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6752.0 3 1719.0 4 1742.0 5 2077.0 6 2618.0 7 3438.0 8 4388.0 9 5611.0 10 7266.0 11 9202.0 12 11511.0 13 13157.0 14 14594.0 15 16910.0 16 19127.0 17 19915.0 18 15837.0 19 14607.0 20 15303.0 21 16725.0 22 18315.0 23 20547.0 24 22819.0 25 25809.0 26 29125.0 27 33010.0 28 37027.0 29 42188.0 30 47956.0 31 55021.0 32 62806.0 33 72758.0 34 85094.0 35 96653.0 36 100786.0 37 60659.0 38 7847.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.29930657449512 19.691188234438563 14.511671878408844 19.497833312657477 2 14.732497782047574 23.15473710524123 40.673571320037055 21.439193792674143 3 19.66614302328546 29.93141644554011 31.444686980891916 18.957753550282515 4 11.308745775485452 17.6174522988032 40.586348881116955 30.487453044594385 5 11.15221111367169 40.65085536928712 34.32440781183347 13.872525705207725 6 25.728363429833745 39.02091632391414 19.377934813422623 15.872785432829495 7 24.137701006898393 37.02598080379215 22.609695419122435 16.226622770187017 8 24.273411373698114 32.76694719473566 21.710596662504035 21.24904476906219 9 27.08603678757544 17.666491095397973 22.66566228130833 32.58180983571825 10-11 23.01172402328819 27.34155602096192 28.477034222732417 21.169685733017477 12-13 27.255553642916098 26.086146261991388 28.144636738272943 18.513663356819563 14-15 23.888131122519297 26.356683818181192 29.876790621549894 19.878394437749616 16-17 18.875329077719233 29.811449746742575 30.570339259020944 20.74288191651725 18-19 18.57195057053802 29.06121788347929 33.854571048107644 18.512260497875044 20-21 20.581531236722835 27.277343477864115 32.831061967134936 19.31006331827811 22-23 19.63442849396807 27.313049430665316 32.47413397582321 20.578388099543403 24-25 20.162828525963526 27.601285586529222 33.28658978932519 18.94929609818206 26-27 20.45688007204855 26.485056551730906 34.33196810821025 18.726095268010297 28-29 19.065102696866333 27.366297759564183 34.59468479301122 18.97391475055827 30-31 19.132939436525003 28.34186852773664 33.76417535198167 18.76101668375669 32-33 18.97071231156827 28.31993968810386 33.097232808925455 19.612115191402413 34-35 19.970800770467065 27.22413475812487 33.062606583322086 19.74245788808598 36-37 20.53053149322324 26.96823445015049 33.623749628310975 18.877484428315295 38-39 20.613426493822555 26.95710626772519 33.76662119859082 18.662846039861435 40-41 19.376709989676275 27.535544233041414 34.28517034146801 18.802575435814298 42-43 18.671567234883334 28.71699611065236 32.744313294876534 19.86712335958778 44-45 19.277054632854693 28.625792376112564 31.822040593603678 20.275112397429066 46-47 19.869916767739436 28.393259546529713 30.745085065559458 20.991738620171393 48-49 20.013743950207633 28.283626046749067 31.0035537928394 20.699076210203902 50-51 20.380383024095604 28.908834924194377 31.215845250882158 19.494936800827865 52-53 19.60900142304508 29.4931739994245 31.18619498958496 19.711629587945463 54-55 18.77395670645397 28.77878302999833 31.539214247032156 20.908046016515552 56-57 19.962528414152814 29.974274485011122 29.591165069845683 20.472032030990388 58-59 19.8420318988015 28.814704437835996 30.216575660175266 21.12668800318723 60-61 19.703863271685208 28.52662777060812 30.993174379634038 20.776334578072632 62-63 21.09373044798077 28.03581058899331 30.56437892604546 20.306080036980457 64-65 20.433396580343572 28.68740128387996 31.04172094673267 19.837481189043793 66-67 19.38579626254626 28.580941360978045 30.743457100543807 21.289805275931887 68-69 19.197278664571268 29.50571532448824 30.586418738472815 20.710587272467677 70-71 19.75419203857397 29.29056147873422 29.765964243705746 21.189282238986067 72-73 19.71285633442244 28.33236889106809 29.75257761306184 22.202197161447636 74-75 19.40082482325216 29.68769638648861 29.638010604870384 21.273468185388843 76-77 20.10962180770917 28.262473727440952 30.251357384865923 21.376547079983954 78-79 19.503470892613432 28.281196304260313 30.876118098716702 21.339214704409557 80-81 19.39348086191939 29.588379435811905 29.78874245570141 21.22939724656729 82-83 19.195517748545416 29.157020184466266 29.75068384476871 21.896778222219602 84-85 19.367459354620873 28.285836837414557 29.51501356481863 22.83169024314594 86-87 19.647163283306494 28.477603266559438 29.892758959699574 21.982474490434495 88-89 20.256539509469913 27.55141858505203 30.021689972302212 22.17035193317584 90-91 19.27400649982283 27.706991677439174 30.73414036358568 22.284861459152314 92-93 19.19044739905499 28.743686335787803 30.294006215020875 21.771860050136336 94-95 19.20180708103979 28.65348700657281 29.571510489592885 22.573195422794516 96-97 18.845712046304705 27.839740778518955 29.334939553129747 23.979607622046593 98-99 19.442841305993262 27.70228155389877 29.135479143018667 23.7193979970893 100-101 19.792768712555 27.614777311828277 29.76297479452216 22.829479181094566 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6827.0 1 4881.0 2 2658.0 3 2105.0 4 1810.5 5 1921.5 6 2653.0 7 5310.0 8 6904.5 9 5614.5 10 4301.0 11 3643.5 12 3421.5 13 3335.5 14 3255.0 15 3250.0 16 3308.5 17 3387.5 18 3554.5 19 3797.5 20 4137.5 21 4577.5 22 5096.0 23 5591.0 24 6268.0 25 7513.0 26 9270.0 27 10754.0 28 11501.5 29 12834.5 30 14687.5 31 16848.0 32 19920.0 33 23484.5 34 26855.0 35 28979.5 36 31681.5 37 33976.5 38 33489.0 39 32093.5 40 30845.0 41 29826.5 42 29224.5 43 29429.0 44 30376.5 45 31488.5 46 35211.0 47 49577.5 48 61460.0 49 50800.0 50 33880.0 51 25459.5 52 21133.5 53 18806.5 54 17285.0 55 15850.5 56 14534.0 57 13401.0 58 12253.5 59 11442.5 60 10241.5 61 8479.0 62 6609.0 63 4736.0 64 3438.0 65 2618.0 66 2009.0 67 1573.5 68 1283.5 69 1038.5 70 842.5 71 700.0 72 613.0 73 529.5 74 422.5 75 361.5 76 285.0 77 210.0 78 164.5 79 126.0 80 86.5 81 53.5 82 43.5 83 35.0 84 23.5 85 15.0 86 7.5 87 7.0 88 5.5 89 4.0 90 3.0 91 3.0 92 4.5 93 3.5 94 2.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 54.20602947643912 2 0.08482287592646465 3 0.1148928792860774 4 0.18600305009740917 5 0.11910463871429677 6 0.14770542366871672 7 0.15505151569468076 8 0.15319050571476986 9 0.13928190481227795 10-11 0.12659765258078007 12-13 0.13884113929072014 14-15 0.14765644972187694 16-17 0.14055522743011173 18-19 0.14873387655235168 20-21 0.17767747913464996 22-23 0.19574886551852144 24-25 0.2225376144398703 26-27 0.2148487047860279 28-29 0.21313461664663633 30-31 0.22777782675172462 32-33 0.2200399431510425 34-35 0.20456417594967827 36-37 0.1913901842497828 38-39 0.1881579037583586 40-41 0.18992096584459 42-43 0.18468075353273564 44-45 0.19805064101999018 46-47 0.224839389941339 48-49 0.22733706123016678 50-51 0.2375236421728369 52-53 0.2656346876588592 54-55 0.25246069595896375 56-57 0.22650450413389087 58-59 0.18458280563905613 60-61 0.19550399578432265 62-63 0.19927498969098417 64-65 0.18908840874831406 66-67 0.19805064101999018 68-69 0.1853663887884923 70-71 0.19839345864786853 72-73 0.21817893317113163 74-75 0.24986507677645645 76-77 0.2488366238928215 78-79 0.24300872421889005 80-81 0.24971815493593716 82-83 0.23948260004642732 84-85 0.20951054458049406 86-87 0.22180300523727386 88-89 0.22307632785510764 90-91 0.21097976298568685 92-93 0.20853106564369886 94-95 0.2461430568166347 96-97 0.2767517735914848 98-99 0.2921295928991695 100-101 0.28972986950402124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1020951.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.13341954114557 #Duplication Level Percentage of deduplicated Percentage of total 1 85.3472377469537 56.44304680597185 2 8.642880065998074 11.431664268969095 3 2.7116197255197783 5.379860548315366 4 1.1613611121307401 3.072191266692546 5 0.5976991418539832 1.976394405380608 6 0.3540422620343016 1.40484152702464 7 0.22950182849766024 1.0624418496647066 8 0.15637047028704987 0.8273051132271767 9 0.1220942567208421 0.7267059632955407 >10 0.6023068724724335 7.228220678337141 >50 0.04916275076293149 2.25623543297312 >100 0.020007374166637587 2.535167026106624 >500 0.002858196300954651 1.304253836203692 >1k 0.0026676498808910075 3.6965200030144056 >5k 1.905464200636434E-4 0.6551512748234777 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5199 0.5092310992398265 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4031 0.3948279594221466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3600 0.3526124172462733 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3170 0.3104948229640796 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2763 0.2706300302365148 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2342 0.2293939669974367 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2116 0.2072577430258651 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2040 0.19981370310622154 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1523 0.1491746420739095 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1450 0.14202444583530455 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1390 0.1361475722145333 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1388 0.13595167642717426 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1227 0.1201820655447715 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1205 0.11802721188382205 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1089 0.10666525621699768 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.794789367952036E-5 0.0 4 0.0 0.0 0.0 9.794789367952036E-5 0.0 5 0.0 0.0 0.0 9.794789367952036E-5 0.0 6 0.0 0.0 0.0 9.794789367952036E-5 0.0 7 0.0 0.0 0.0 1.9589578735904073E-4 0.0 8 0.0 0.0 0.0 1.9589578735904073E-4 0.0 9 0.0 0.0 0.0 1.9589578735904073E-4 0.0 10-11 0.0 0.0 0.0 2.9384368103856106E-4 0.0 12-13 0.0 0.0 0.0 4.897394683976018E-4 0.0 14-15 0.0 0.0 0.0 5.876873620771222E-4 0.0 16-17 0.0 0.0 0.0 0.0012243486709940046 0.0 18-19 0.0 0.0 0.0 0.001665114192551846 0.0 20-21 0.0 0.0 0.0 0.0025466452356675295 0.0 22-23 0.0 0.0 0.0 0.005387134152373621 0.0 24-25 0.0 0.0 0.0 0.009549919633753236 0.0 26-27 0.0 0.0 0.0 0.012929121965696688 0.0 28-29 0.0 0.0 0.0 0.024682869207239132 0.0 30-31 0.0 0.0 0.0 0.05323468021481932 0.0 32-33 0.0 0.0 0.0 0.10490219413076632 0.0 34-35 0.0 0.0 0.0 0.1860030500974092 0.0 36-37 0.0 0.0 0.0 0.29918184124409497 0.0 38-39 0.0 0.0 0.0 0.4512949201283901 0.0 40-41 0.0 0.0 0.0 0.630196747934034 0.0 42-43 0.0 0.0 0.0 0.7903415541000498 0.0 44-45 0.0 0.0 0.0 0.9766384478784976 0.0 46-47 0.0 0.0 0.0 1.1999106715209642 0.0 48-49 0.0 0.0 0.0 1.4569749184828655 0.0 50-51 0.0 0.0 0.0 1.715165566222081 0.0 52-53 0.0 0.0 0.0 1.9503874328934492 0.0 54-55 0.0 0.0 0.0 2.1848257164153813 0.0 56-57 0.0 0.0 0.0 2.4509011695957987 0.0 58-59 0.0 0.0 0.0 2.7713866777151894 0.0 60-61 0.0 0.0 0.0 3.186147033501118 0.0 62-63 0.0 0.0 0.0 3.565401277828221 0.0 64-65 0.0 0.0 9.794789367952036E-5 3.8995505171159044 0.0 66-67 0.0 0.0 9.794789367952036E-5 4.273074809662756 4.897394683976018E-5 68-69 0.0 0.0 9.794789367952036E-5 4.687198504139768 9.794789367952036E-5 70-71 0.0 0.0 9.794789367952036E-5 5.174048509673824 9.794789367952036E-5 72-73 0.0 0.0 9.794789367952036E-5 5.694641564580474 9.794789367952036E-5 74-75 0.0 0.0 9.794789367952036E-5 6.20201165384039 9.794789367952036E-5 76-77 0.0 0.0 9.794789367952036E-5 6.689204477002324 9.794789367952036E-5 78-79 0.0 0.0 9.794789367952036E-5 7.246576965985635 9.794789367952036E-5 80-81 0.0 0.0 9.794789367952036E-5 7.873933225002963 9.794789367952036E-5 82-83 0.0 0.0 9.794789367952036E-5 8.504668686352234 9.794789367952036E-5 84-85 0.0 9.794789367952036E-5 9.794789367952036E-5 9.215133733156636 9.794789367952036E-5 86-87 0.0 9.794789367952036E-5 9.794789367952036E-5 9.942543765567592 9.794789367952036E-5 88-89 0.0 9.794789367952036E-5 9.794789367952036E-5 10.611527879398718 9.794789367952036E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATATG 435 0.0 40.300236 1 GTACATG 6150 0.0 35.715145 1 CATTCCG 185 0.0 33.164776 9 CATATAG 230 0.0 28.750576 3 TACATGG 7575 0.0 28.495739 2 ACATGGG 7500 0.0 27.71008 3 ATGGGAT 1975 0.0 27.00197 5 CGCGTCA 35 0.0086310655 26.98769 26-27 CATGGGA 5830 0.0 26.085012 4 ATATGAG 460 0.0 23.616545 3 GAGTACT 7485 0.0 23.457293 12-13 CATGGGG 1390 0.0 23.444344 4 AGTACTT 7860 0.0 23.208853 12-13 TAGGACA 495 0.0 22.898645 4 ATGGGAG 2015 0.0 22.718561 5 CCTTTAG 320 2.6456282E-5 22.557762 1 GCCGTAT 130 6.657501E-10 21.816486 94-95 TAGGCAT 325 1.8189894E-12 21.781715 5 TTTGCGT 265 8.5128704E-10 21.38857 3 ATAGCCC 310 1.2732926E-11 21.331074 3 >>END_MODULE