FastQCFastQC Report
Fri 27 May 2016
SRR939687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991160
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142940.4778748044237019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92720.3099800746198799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69690.23298653365249603No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG35840.11981973548723572No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG34020.11373513954452454No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171000.032.4423641
GTACATG129400.030.7329181
GGTATCA113450.030.7033391
TACATGG135950.028.6248512
ACATGGG135350.027.8751663
ATCAACG206900.027.0434423
TCAACGC207800.026.9021244
TATCAAC208300.026.816112
CAACGCA210400.026.5245725
AACGCAG215700.025.9780546
CATGGGA86050.023.6588274
ACGCAGA236300.023.4129547
CATGGGG58900.023.36524
CGCAGAG236800.023.1246198
GAGTACT106050.022.82046512-13
AGTACTT109600.021.75657312-13
GCAGAGT239750.021.0785879
ATGGGAG36400.020.72925
GTACTTT117800.020.72585114-15
AGAGTAC184200.019.69386910-11
Produced by FastQC (version 0.11.5)