##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939687_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2991160 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.265711295952073 31.0 30.0 33.0 26.0 34.0 2 30.406759919228662 31.0 30.0 34.0 26.0 34.0 3 30.45416694526538 31.0 30.0 34.0 26.0 34.0 4 34.05103872745022 35.0 35.0 37.0 28.0 37.0 5 34.05107784270985 35.0 35.0 37.0 28.0 37.0 6 34.1670850104976 36.0 35.0 37.0 30.0 37.0 7 33.97341432755185 36.0 35.0 37.0 29.0 37.0 8 33.97704469169152 36.0 35.0 37.0 29.0 37.0 9 35.2480933818318 38.0 35.0 39.0 28.0 39.0 10-11 35.139903916875056 37.0 34.5 39.0 28.5 39.0 12-13 34.96697986734243 37.0 34.0 39.0 27.0 39.0 14-15 35.66412328327472 38.0 34.0 40.0 27.0 41.0 16-17 35.38858486339748 38.0 34.0 40.0 27.0 41.0 18-19 35.37103598603886 38.0 34.0 40.0 27.0 41.0 20-21 35.49327200818412 38.0 34.0 40.0 27.0 41.0 22-23 35.33944790649781 38.0 34.0 40.0 27.0 41.0 24-25 35.19231752898541 38.0 34.0 40.0 26.0 41.0 26-27 35.32231776300833 38.0 34.0 40.0 26.0 41.0 28-29 35.19324743577742 38.0 34.0 40.0 25.5 41.0 30-31 34.941592057930706 38.0 34.0 40.0 25.0 41.0 32-33 34.69187522566496 38.0 33.5 40.0 24.0 41.0 34-35 34.43500397838966 38.0 33.0 40.0 24.0 41.0 36-37 34.119010183340244 38.0 33.0 40.0 23.0 41.0 38-39 33.94450363738483 38.0 33.0 40.0 22.5 41.0 40-41 33.6371834004199 38.0 32.5 40.0 21.0 41.0 42-43 33.28019213281804 37.0 31.5 40.0 18.5 41.0 44-45 33.000935590205806 37.0 31.0 40.0 17.0 41.0 46-47 32.48947582208909 37.0 31.0 40.0 14.0 41.0 48-49 32.08378990090801 37.0 30.0 39.5 10.5 41.0 50-51 31.422774274863265 35.5 29.5 38.5 9.0 40.5 52-53 31.440469750865883 36.0 30.0 39.0 8.0 40.0 54-55 31.44100215301087 36.0 30.0 39.0 7.0 40.0 56-57 31.29060999745918 36.0 29.5 39.0 6.0 40.0 58-59 30.877085144224985 35.0 29.0 39.0 2.0 40.0 60-61 30.44464455261504 35.0 28.0 38.5 2.0 40.0 62-63 30.026315543133766 35.0 28.0 38.0 2.0 40.0 64-65 29.40333248639324 34.0 27.0 38.0 2.0 40.0 66-67 28.863618796721006 34.0 26.0 37.0 2.0 39.5 68-69 28.35116727289747 34.0 26.0 36.5 2.0 39.0 70-71 27.795628284678855 33.0 25.5 36.0 2.0 39.0 72-73 27.062958183447222 32.5 24.0 35.5 2.0 38.0 74-75 26.614932166784794 32.0 23.0 35.0 2.0 37.5 76-77 26.237962195268725 32.0 22.0 35.0 2.0 37.0 78-79 25.674571069417887 31.5 20.5 35.0 2.0 36.5 80-81 25.016582028376952 31.0 18.5 34.0 2.0 36.0 82-83 24.3731214645823 31.0 16.0 34.0 2.0 35.5 84-85 23.726391934901507 30.0 8.5 34.0 2.0 35.0 86-87 23.049038834432125 30.0 4.5 34.0 2.0 35.0 88-89 22.267189150697387 29.0 2.0 34.0 2.0 35.0 90-91 21.040237232378075 28.0 2.0 33.5 2.0 35.0 92-93 19.411406611481834 25.0 2.0 32.5 2.0 34.0 94-95 19.119490264646494 25.0 2.0 32.0 2.0 34.0 96-97 18.475416560799154 25.0 2.0 32.0 2.0 34.0 98-99 17.48043050187887 22.5 2.0 32.0 2.0 34.0 100-101 15.865631728158975 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9434423480603833 1101 2 0.9609360058738936 1101 3 0.9040624751813695 1101 4 1.1321778493670394 1101 5 1.0320900968720608 1101 6 0.8951424162955988 1101 7 0.9191267166608199 1101 8 0.8822020626827793 1101 9 1.186665957806099 1101 10-11 0.9659708674498759 1101 12-13 0.8198952752865551 1101 14-15 1.0488064598533455 1101 16-17 0.9950223051847402 1101 18-19 1.0878554691351283 1101 20-21 1.0998764229135887 1101 22-23 1.2415344069869576 1101 24-25 0.9672194135150178 1101 26-27 0.946332224398823 1101 28-29 1.0600233280314981 1101 30-31 1.2125098039676203 1101 32-33 0.9984326301581277 1101 34-35 1.0215701061477063 1101 36-37 1.0904400091747704 1101 38-39 1.0527026671186164 1101 40-41 1.2152202632619051 1101 42-43 1.1779970773913604 1101 44-45 1.0837498047424674 1101 46-47 0.9918416746312531 1101 48-49 1.0642184723854697 1101 50-51 1.0531583421834974 1101 52-53 1.0270077317134003 1101 54-55 1.0935422024161596 1101 56-57 0.9073982985682392 1101 58-59 0.934528419476468 1101 60-61 0.8952256371047831 1101 62-63 0.8949599969141566 1101 64-65 0.7780294413865434 1101 66-67 0.6009044223699433 1101 68-69 0.529175459006094 1101 70-71 0.41116315848229235 1101 72-73 0.5827856674512795 1101 74-75 0.46688013544019213 1101 76-77 -0.12014232841874417 1101 78-79 -0.002188273224259518 1101 80-81 -0.2194739038725224 1101 82-83 -0.17762939886776863 1101 84-85 -0.37577557695629693 1101 86-87 -0.8607742529274489 1101 88-89 -1.2331938018521846 1101 90-91 -4.310170384177255 1101 92-93 -9.4687590646874 1101 94-95 -7.782031696239946 1101 96-97 -4.447320825802677 1101 98-99 -3.1214889800481416 1101 100-101 -2.5106585997452306 1102 1 0.9100996329178059 1102 2 0.8985778066350996 1102 3 0.8531171955014152 1102 4 0.9568962886450763 1102 5 0.8056232054824335 1102 6 0.8522133714034226 1102 7 0.7445488650422831 1102 8 0.6935967178117295 1102 9 0.8670515017153875 1102 10-11 0.7082320860896374 1102 12-13 0.7462538721143801 1102 14-15 0.9060941655869499 1102 16-17 0.9952281470714581 1102 18-19 0.9558245629008084 1102 20-21 1.013087199573235 1102 22-23 1.0747542483242611 1102 24-25 0.958438319020523 1102 26-27 0.9321259041719756 1102 28-29 1.0130320625105895 1102 30-31 0.9377370192014425 1102 32-33 0.8507213291302307 1102 34-35 0.8744176761196343 1102 36-37 0.7915467983892981 1102 38-39 0.7101640758024175 1102 40-41 0.7242726814415903 1102 42-43 0.7840289200447614 1102 44-45 0.702559847771056 1102 46-47 0.772761820165293 1102 48-49 0.6973291676039608 1102 50-51 0.6023395040390831 1102 52-53 0.8280641175615138 1102 54-55 0.8820980248418593 1102 56-57 0.5598063770254207 1102 58-59 0.42700851029355746 1102 60-61 0.446174073418522 1102 62-63 0.40768548884616607 1102 64-65 0.38823464754189274 1102 66-67 0.45057296848168704 1102 68-69 0.25026542850126887 1102 70-71 0.41539458936383156 1102 72-73 0.3360749165585766 1102 74-75 0.15656835251910906 1102 76-77 -0.07922193035400937 1102 78-79 -0.12597625431910942 1102 80-81 -0.2106381922725653 1102 82-83 -0.6248404248280721 1102 84-85 -0.6671435930325202 1102 86-87 -0.9974585512290481 1102 88-89 -1.638099401617744 1102 90-91 -5.607518386020729 1102 92-93 -10.683857302801243 1102 94-95 -9.209175634189041 1102 96-97 -5.985558745842354 1102 98-99 -4.660342884136099 1102 100-101 -3.972118156732968 1103 1 0.5611107168248672 1103 2 0.6914629009075988 1103 3 0.5974709597748245 1103 4 0.6624989281355909 1103 5 0.584213268330771 1103 6 0.46449669811012484 1103 7 0.5972689214034972 1103 8 0.5202629659545366 1103 9 0.5164020921828083 1103 10-11 0.4198862319173031 1103 12-13 0.37382500373316674 1103 14-15 0.4426860559894976 1103 16-17 0.6476590731950225 1103 18-19 0.48253998759789596 1103 20-21 0.5810540803846109 1103 22-23 0.5944147557146948 1103 24-25 0.41665847652500787 1103 26-27 0.49745922355450745 1103 28-29 0.6890991239847679 1103 30-31 0.6960148981224847 1103 32-33 0.7119236435672107 1103 34-35 0.7183314671785723 1103 36-37 0.6476954371404275 1103 38-39 0.6303789529307835 1103 40-41 0.585791504552482 1103 42-43 0.4347165198801406 1103 44-45 0.4022084621082911 1103 46-47 0.3896143811825823 1103 48-49 0.053416422854688506 1103 50-51 0.1229629650919506 1103 52-53 0.2884782120502578 1103 54-55 0.3019118165983343 1103 56-57 0.3126190317920603 1103 58-59 0.08507782482121584 1103 60-61 -0.009751178218952106 1103 62-63 0.24810024599769065 1103 64-65 0.12977941913249325 1103 66-67 0.10795747333307304 1103 68-69 0.24979127583771188 1103 70-71 0.37894273007155377 1103 72-73 0.27813133357575737 1103 74-75 0.0608643256219743 1103 76-77 -0.19188332361996174 1103 78-79 -0.19055169673370997 1103 80-81 -0.439135831100419 1103 82-83 -0.4484561571402068 1103 84-85 -0.4746002932508482 1103 86-87 -0.7642274074730757 1103 88-89 -1.0156093241210726 1103 90-91 -2.512961986442047 1103 92-93 -9.22291639139577 1103 94-95 -5.977339395602536 1103 96-97 -3.346572550154546 1103 98-99 -2.3596350556471783 1103 100-101 -2.0455166054045044 1104 1 1.0205200546103441 1104 2 0.851023293566584 1104 3 0.9105905567386614 1104 4 0.9873279976208309 1104 5 0.9395198553274966 1104 6 0.8616965340837766 1104 7 0.9845706880341751 1104 8 0.9294304485685743 1104 9 1.003165751978777 1104 10-11 0.9250739835010791 1104 12-13 0.9811718776086167 1104 14-15 0.9226604059514258 1104 16-17 1.0953080986609436 1104 18-19 1.0276631328867367 1104 20-21 1.085952693692498 1104 22-23 1.0549174629862534 1104 24-25 0.8876906708794863 1104 26-27 0.9262155105336731 1104 28-29 0.9959972268025012 1104 30-31 1.0731903393617301 1104 32-33 1.1605610114464966 1104 34-35 1.0550187626686807 1104 36-37 1.0667327447651402 1104 38-39 0.9523484406832026 1104 40-41 0.8623769530724772 1104 42-43 0.97174337500973 1104 44-45 0.9464225583314914 1104 46-47 0.921576086052319 1104 48-49 0.9157801177991658 1104 50-51 0.7648265052156411 1104 52-53 0.9809129153383118 1104 54-55 1.1036876528397812 1104 56-57 1.120681637969824 1104 58-59 0.8517781754693736 1104 60-61 0.6645860214496473 1104 62-63 0.8566695764187209 1104 64-65 0.8466023711045736 1104 66-67 0.8426582507844742 1104 68-69 0.5328135030811474 1104 70-71 0.6617444423057428 1104 72-73 0.7535518603011724 1104 74-75 0.5162742659532036 1104 76-77 0.5141151449095744 1104 78-79 0.44723712771625657 1104 80-81 0.36672057889849086 1104 82-83 0.50890431585821 1104 84-85 0.4425655658371319 1104 86-87 0.19396993463064405 1104 88-89 0.04349643939645276 1104 90-91 -0.34509001904646297 1104 92-93 -3.8520660068544146 1104 94-95 -1.4532748060449912 1104 96-97 -0.5797052441198822 1104 98-99 -0.2930292712079243 1104 100-101 -0.2906613000728111 1105 1 1.0254898017921867 1105 2 0.9408093061363587 1105 3 0.9889742277486775 1105 4 0.9504074248676204 1105 5 0.8231021572196582 1105 6 0.7693986588944384 1105 7 0.9541316665015316 1105 8 0.7823544692225042 1105 9 1.1013694124442495 1105 10-11 0.9403110685186107 1105 12-13 0.952746572360617 1105 14-15 1.1018363827868853 1105 16-17 1.1545218182930554 1105 18-19 0.9954484843299198 1105 20-21 1.0237011392081428 1105 22-23 0.9689245596362639 1105 24-25 1.1254820059367745 1105 26-27 1.0350409229009188 1105 28-29 1.1624651370566639 1105 30-31 1.2241194732858887 1105 32-33 1.121237369057404 1105 34-35 1.229115780904067 1105 36-37 1.337728116878985 1105 38-39 1.2333201294013705 1105 40-41 1.1333405241603245 1105 42-43 1.165762271514133 1105 44-45 1.0128293771409673 1105 46-47 1.029973858306768 1105 48-49 0.9632203444233127 1105 50-51 1.0237799585559948 1105 52-53 0.988968408128688 1105 54-55 1.2365523394741444 1105 56-57 1.1323902736221214 1105 58-59 1.014489589527102 1105 60-61 0.9381246387745108 1105 62-63 1.060681945344097 1105 64-65 1.0753676544266106 1105 66-67 1.1370424406533388 1105 68-69 1.146523301981503 1105 70-71 0.8879296581761302 1105 72-73 1.0416934250790284 1105 74-75 0.9631519073213219 1105 76-77 0.6066460881447426 1105 78-79 0.5766051660113867 1105 80-81 0.5255700512525188 1105 82-83 0.6832431892650241 1105 84-85 0.433406242696865 1105 86-87 0.06926932455306911 1105 88-89 -0.38211259209493065 1105 90-91 -1.3900861694485798 1105 92-93 -7.570792208389236 1105 94-95 -3.2685158661907714 1105 96-97 -1.4393830076708838 1105 98-99 -0.7629030295033878 1105 100-101 -0.5430656250123462 1106 1 0.7838382958289785 1106 2 0.7694697737598339 1106 3 0.6138377814175691 1106 4 0.686001387538596 1106 5 0.5264240357992662 1106 6 0.5700387774848323 1106 7 0.6301103741357892 1106 8 0.6809057797810922 1106 9 0.8939608820217657 1106 10-11 0.7068547650350183 1106 12-13 0.746598064847646 1106 14-15 0.7088642111003409 1106 16-17 0.8358606768605412 1106 18-19 0.7451435857381838 1106 20-21 0.6807239139537771 1106 22-23 0.7044578794934182 1106 24-25 0.45763954249091654 1106 26-27 0.5459869507966104 1106 28-29 0.5606441681192607 1106 30-31 0.6769781081487665 1106 32-33 0.5617063401509981 1106 34-35 0.7357140559529114 1106 36-37 0.7639224434068481 1106 38-39 0.6599810686911738 1106 40-41 0.6217830145585452 1106 42-43 0.7852707278176112 1106 44-45 0.7526717058250227 1106 46-47 0.7490578149134635 1106 48-49 0.5765230194452116 1106 50-51 0.6303445431527273 1106 52-53 0.7483208776388288 1106 54-55 0.6626068506256928 1106 56-57 0.7381176504835238 1106 58-59 0.6986904520026762 1106 60-61 0.7360745747626503 1106 62-63 0.5876696820195235 1106 64-65 0.60505736536253 1106 66-67 0.2962264579028471 1106 68-69 0.34837796137103183 1106 70-71 0.32781578444794235 1106 72-73 0.60966559676557 1106 74-75 0.542086952129619 1106 76-77 0.4199622393474911 1106 78-79 0.19505911116332797 1106 80-81 0.06335389331168173 1106 82-83 0.19253333143872808 1106 84-85 0.0019679972956083702 1106 86-87 -0.22147759277056167 1106 88-89 -0.2485451101193199 1106 90-91 -0.6692216724470299 1106 92-93 -4.204002571572307 1106 94-95 -1.8585822363814621 1106 96-97 -0.8898984715884097 1106 98-99 -0.6627733212627014 1106 100-101 -0.6075558884716816 1107 1 0.6773653346140129 1107 2 0.5082574448121164 1107 3 0.481365532096504 1107 4 0.5038316931256475 1107 5 0.3413388721955002 1107 6 0.48021138978389644 1107 7 0.480737236265675 1107 8 0.4697727698761014 1107 9 0.544975550551662 1107 10-11 0.39713829216169216 1107 12-13 0.42507535894771564 1107 14-15 0.6887893523805175 1107 16-17 0.6623898205664247 1107 18-19 0.5443686320991716 1107 20-21 0.45280031511038743 1107 22-23 0.46452700351236587 1107 24-25 0.6052489851922473 1107 26-27 0.46806521957611125 1107 28-29 0.45718664884978466 1107 30-31 0.5831738921548819 1107 32-33 0.6199266273694306 1107 34-35 0.5813287675480012 1107 36-37 0.6183582674899597 1107 38-39 0.5614282567102649 1107 40-41 0.3905869009719183 1107 42-43 0.4822193615965418 1107 44-45 0.3653762654615136 1107 46-47 0.5199177344079331 1107 48-49 0.5301569741476655 1107 50-51 0.49676589205983746 1107 52-53 0.5896255550454192 1107 54-55 0.7226421377122847 1107 56-57 0.607777457481177 1107 58-59 0.5449847586092282 1107 60-61 0.4503511235713269 1107 62-63 0.48911687003861104 1107 64-65 0.6817402034462177 1107 66-67 0.43585247191221654 1107 68-69 0.42165117921935646 1107 70-71 0.25571169852850417 1107 72-73 0.4621573352807893 1107 74-75 0.32005372601981463 1107 76-77 0.1812517999947012 1107 78-79 0.006652764761032159 1107 80-81 -0.0768951377216176 1107 82-83 0.20387760245058573 1107 84-85 -0.14078386813007526 1107 86-87 -0.3626040769814587 1107 88-89 -0.3638652206825981 1107 90-91 -0.33211870710132274 1107 92-93 -3.91911559571518 1107 94-95 -1.3420488301009748 1107 96-97 -0.5019576027263 1107 98-99 -0.24572840603081048 1107 100-101 0.04192656026756225 1108 1 0.3291020159086031 1108 2 0.37445609818650993 1108 3 0.2501063729144377 1108 4 -0.09453721965685702 1108 5 -0.7269414499668088 1108 6 -0.1729516145482748 1108 7 0.14662302201809752 1108 8 0.24148991827392052 1108 9 0.4726825530851215 1108 10-11 0.3423931834158509 1108 12-13 0.2940318044021524 1108 14-15 0.2688560111510512 1108 16-17 0.34944696784005913 1108 18-19 0.4026664997412155 1108 20-21 0.38543942426575484 1108 22-23 0.3114148891013855 1108 24-25 0.20047456706378597 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56-57 0.318051113246014 2311 58-59 0.23923679870079795 2311 60-61 0.1950234133816906 2311 62-63 0.12786081530288484 2311 64-65 0.37462564129238984 2311 66-67 0.278793046338599 2311 68-69 0.3431491871389021 2311 70-71 0.23904345689387085 2311 72-73 0.4091059999619766 2311 74-75 0.6477953515086874 2311 76-77 0.5005872879858231 2311 78-79 0.5675954516767057 2311 80-81 0.6137295860239185 2311 82-83 0.7388904740033091 2311 84-85 0.7266574287493519 2311 86-87 0.8507388611162021 2311 88-89 1.2323576377928127 2311 90-91 1.9173674595993866 2311 92-93 2.8879802954474236 2311 94-95 2.3405456527354325 2311 96-97 1.8677882279270506 2311 98-99 1.741539311596739 2311 100-101 1.67266407966253 2312 1 0.31292205526483485 2312 2 0.1460498294011039 2312 3 0.10912054274596628 2312 4 -0.028234066440795402 2312 5 0.05421062361524065 2312 6 0.21726995459113851 2312 7 0.3114171771552634 2312 8 -0.44209475904560946 2312 9 0.04296855135598321 2312 10-11 0.11469510260432259 2312 12-13 0.10428488281521453 2312 14-15 -0.18028747935343148 2312 16-17 0.22950144814956275 2312 18-19 0.07975982763149858 2312 20-21 0.30012617416311116 2312 22-23 0.25510329573448587 2312 24-25 0.37525467708001514 2312 26-27 0.3564963720844574 2312 28-29 0.21325558613325057 2312 30-31 0.3900355560732578 2312 32-33 0.368666355528525 2312 34-35 0.19917628849498215 2312 36-37 0.3240489092046417 2312 38-39 0.48892940574108223 2312 40-41 0.5864583229013647 2312 42-43 0.6080291524805403 2312 44-45 0.439502402792769 2312 46-47 0.45346423134778036 2312 48-49 0.20272456721726684 2312 50-51 0.39696852288384576 2312 52-53 0.39794605055925913 2312 54-55 0.4805652188498435 2312 56-57 0.3988850337794858 2312 58-59 0.383576413982162 2312 60-61 0.5302106678702678 2312 62-63 0.40114643323188304 2312 64-65 0.44153245715222056 2312 66-67 0.3692672298053381 2312 68-69 0.17409344042069463 2312 70-71 0.2990034321268489 2312 72-73 0.46768072072127254 2312 74-75 0.7788188812417012 2312 76-77 0.6797220575982763 2312 78-79 0.7788683910475065 2312 80-81 0.8000513661428492 2312 82-83 1.076682982121568 2312 84-85 1.0700621457602608 2312 86-87 1.0313433746432032 2312 88-89 1.1971067014937766 2312 90-91 1.553236410004704 2312 92-93 2.7523840074506687 2312 94-95 2.339630586876222 2312 96-97 1.9890708255136822 2312 98-99 1.9946331943484452 2312 100-101 1.8136866643195315 2313 1 -0.2425942783128825 2313 2 -0.9240873855970442 2313 3 -0.294504973060274 2313 4 -0.1536108075982625 2313 5 -0.7056734470746164 2313 6 -0.32497972869849434 2313 7 -1.1050676702179416 2313 8 -1.1429098550410828 2313 9 -1.072466844050055 2313 10-11 -2.063345594699932 2313 12-13 -0.40727489127324645 2313 14-15 -0.055937985605005736 2313 16-17 -1.5548173807337164 2313 18-19 -1.2088735902813923 2313 20-21 -1.4986027339317118 2313 22-23 -1.1439073227709144 2313 24-25 -0.31247220756051775 2313 26-27 -0.8031507853176336 2313 28-29 -2.157446160795814 2313 30-31 -0.562246954336338 2313 32-33 -0.22748643581724082 2313 34-35 0.10891797840823614 2313 36-37 0.13876584870754272 2313 38-39 -0.3152555802739201 2313 40-41 -0.20866785595520554 2313 42-43 -0.030166708653318608 2313 44-45 -0.5560375057117568 2313 46-47 -0.8004030919200744 2313 48-49 -0.2828934399562719 2313 50-51 -0.1932647431421053 2313 52-53 -0.10301682303154891 2313 54-55 0.1435073898207051 2313 56-57 -0.08842247748108178 2313 58-59 0.012755355031089266 2313 60-61 -0.11510352662719825 2313 62-63 -0.0628781702650798 2313 64-65 0.08450877975640836 2313 66-67 0.05197070794918801 2313 68-69 0.1228448264419093 2313 70-71 -0.08244684650596312 2313 72-73 0.21631723034110095 2313 74-75 0.08099994556843271 2313 76-77 0.04100194667072543 2313 78-79 0.17441763816483657 2313 80-81 0.12802093478711996 2313 82-83 0.1830381418241913 2313 84-85 0.5084178656032883 2313 86-87 0.4495362445990949 2313 88-89 0.6353148906547368 2313 90-91 1.2888434383558156 2313 92-93 2.349343411557225 2313 94-95 1.562041820230963 2313 96-97 0.9491666860655315 2313 98-99 0.9372816778511996 2313 100-101 0.8319459010365211 2314 1 -0.5468199916904233 2314 2 -0.6326423933840317 2314 3 -1.8045292606031822 2314 4 -0.5211565630463895 2314 5 -0.2586685220665501 2314 6 -2.7326085207890287 2314 7 -3.9383586629635836 2314 8 -1.583359352538082 2314 9 -1.7594244703262518 2314 10-11 -1.0319202877920262 2314 12-13 -2.0811075489980766 2314 14-15 -2.968570330623116 2314 16-17 -2.071452629457063 2314 18-19 -2.6371191804171374 2314 20-21 -2.0497072096386475 2314 22-23 -3.1909808518036726 2314 24-25 -2.0310272644380447 2314 26-27 -2.114392763617168 2314 28-29 -1.3094721885982352 2314 30-31 -1.397067117292373 2314 32-33 -2.366821332684161 2314 34-35 -0.7621945113287723 2314 36-37 -0.375810052011758 2314 38-39 -0.5398263755774266 2314 40-41 -0.7858510655190258 2314 42-43 -0.5247366816903423 2314 44-45 -1.0865033994479312 2314 46-47 -1.5292551956336524 2314 48-49 -1.7562771647252688 2314 50-51 -0.8251878894527671 2314 52-53 -0.3638330022905514 2314 54-55 -0.10390369801330834 2314 56-57 -0.39123192888206404 2314 58-59 -0.32564718982231966 2314 60-61 -0.3499864027435464 2314 62-63 -0.3190292144821534 2314 64-65 -0.44617618049866437 2314 66-67 -0.3427926818014413 2314 68-69 -0.33001905409136256 2314 70-71 -0.4116139273740309 2314 72-73 -0.1715363595884618 2314 74-75 -0.10483688239746769 2314 76-77 -0.24834503874079417 2314 78-79 -0.2835013403592761 2314 80-81 -0.15345837171086885 2314 82-83 -0.16051706846125668 2314 84-85 -0.16792515942920616 2314 86-87 0.035612818472372965 2314 88-89 0.24806542113934782 2314 90-91 0.8683516984522051 2314 92-93 1.6132995442538522 2314 94-95 1.1520281485165604 2314 96-97 0.9311286752631602 2314 98-99 1.0052904797843851 2314 100-101 1.0658567845311797 2315 1 -7.073054464399139 2315 2 -2.9276101828713657 2315 3 -1.4345551840160269 2315 4 -0.7734487842827065 2315 5 -1.420153933095257 2315 6 -1.554309005989694 2315 7 -3.072606301412904 2315 8 -2.8514542057626393 2315 9 -3.50299778898178 2315 10-11 -2.0984441305307016 2315 12-13 -2.8418167074967826 2315 14-15 -4.580219469857198 2315 16-17 -4.291259524547108 2315 18-19 -3.1706685685518394 2315 20-21 -4.214341047363455 2315 22-23 -5.397355891938304 2315 24-25 -3.2585940487275167 2315 26-27 -4.3544421430527365 2315 28-29 -2.8309246430978234 2315 30-31 -2.4670421428757265 2315 32-33 -1.5707412405682604 2315 34-35 -2.169057124621787 2315 36-37 -2.817084717345608 2315 38-39 -1.9511867036348747 2315 40-41 -3.3561871050731895 2315 42-43 -7.55053303448706 2315 44-45 -4.081436814184048 2315 46-47 -2.724348779839403 2315 48-49 -5.2636216044774535 2315 50-51 -6.450299066993612 2315 52-53 -6.681679244871905 2315 54-55 -4.479297333728464 2315 56-57 -3.591228264702295 2315 58-59 -3.971175888785435 2315 60-61 -2.904314458076353 2315 62-63 -3.0359758086665956 2315 64-65 -1.9506127377302533 2315 66-67 -2.604549953880234 2315 68-69 -1.879486799028598 2315 70-71 -1.4983151427745547 2315 72-73 -1.9342572582245978 2315 74-75 -2.7215628876875932 2315 76-77 -2.7212950883258458 2315 78-79 -1.7754893081419105 2315 80-81 -1.470359835853838 2315 82-83 -1.8233076128740144 2315 84-85 -1.79804057845584 2315 86-87 -2.230804947580026 2315 88-89 -1.7156390329743125 2315 90-91 -0.6594860506131646 2315 92-93 0.3561982847777294 2315 94-95 0.19180796030952507 2315 96-97 0.04953559007124397 2315 98-99 0.059660844531073565 2315 100-101 -0.014961049848947638 2316 1 -4.14376992030606 2316 2 -2.8527960773422727 2316 3 -4.2035456330029035 2316 4 -4.259741146447606 2316 5 -3.9298905927381895 2316 6 -4.363859297803668 2316 7 -2.7406544522960097 2316 8 -1.5876694331052406 2316 9 -1.9389010508245192 2316 10-11 -3.030727690940658 2316 12-13 -3.9053876575579594 2316 14-15 -2.982181786949397 2316 16-17 -2.6241753560749217 2316 18-19 -2.81819642229231 2316 20-21 -5.327978222470776 2316 22-23 -6.364488613422537 2316 24-25 -4.143088416668281 2316 26-27 -3.7278886495350356 2316 28-29 -4.159527236018008 2316 30-31 -5.96781249930082 2316 32-33 -4.303198050026769 2316 34-35 -5.9287895916647955 2316 36-37 -8.93475643586978 2316 38-39 -5.803456973953878 2316 40-41 -5.257830739489293 2316 42-43 -4.287021457050642 2316 44-45 -3.186672244448488 2316 46-47 -5.626582669210666 2316 48-49 -3.028468141795649 2316 50-51 -4.352768733758555 2316 52-53 -5.253906020347809 2316 54-55 -6.759147968974975 2316 56-57 -4.818376882000873 2316 58-59 -5.491212504158124 2316 60-61 -5.777081357635183 2316 62-63 -5.911124961536007 2316 64-65 -4.844087699793988 2316 66-67 -6.170621677092903 2316 68-69 -4.859401450316987 2316 70-71 -5.579078186944724 2316 72-73 -7.544280817931277 2316 74-75 -7.105653819319361 2316 76-77 -6.635248196124351 2316 78-79 -5.810485841357671 2316 80-81 -5.8075376480740495 2316 82-83 -4.764967359496719 2316 84-85 -6.186160688779825 2316 86-87 -4.822931425246093 2316 88-89 -4.790028874439937 2316 90-91 -5.392191756245767 2316 92-93 -4.1578718100832255 2316 94-95 -4.051648112513682 2316 96-97 -4.83472949224347 2316 98-99 -5.905990942463211 2316 100-101 -5.0993293242199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14337.0 3 5682.0 4 6044.0 5 7299.0 6 8537.0 7 10099.0 8 12159.0 9 15260.0 10 18149.0 11 21697.0 12 26222.0 13 30051.0 14 32361.0 15 35890.0 16 41069.0 17 45507.0 18 41338.0 19 38847.0 20 41814.0 21 44680.0 22 49447.0 23 55070.0 24 62374.0 25 70356.0 26 79608.0 27 89998.0 28 102592.0 29 117073.0 30 132919.0 31 154028.0 32 176743.0 33 204712.0 34 241301.0 35 286171.0 36 320611.0 37 270774.0 38 79170.0 39 1171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.07282368797573 18.35508942684639 13.258669311393875 23.313417573784008 2 15.820999112853057 20.5035688338686 39.46291300772932 24.212519045549023 3 18.871845462023067 25.445886878018843 29.666159580285072 26.016108079673018 4 12.631041056714546 16.150991155115598 36.89612857693102 34.32183921123884 5 13.800127294667075 37.62193216171375 33.51627758804046 15.061662955578711 6 32.1660996114537 35.831911652474396 17.502316121375802 14.499672614696104 7 28.623911355101267 33.02931975400064 21.135714245081836 17.211054645816258 8 26.92463156316796 33.90686254170888 19.930488541015574 19.23801735410758 9 27.721967596635594 15.776413975029962 19.927948654633347 36.57366977370109 10-11 25.394388029536337 25.9025153733994 27.41138806885342 21.291708528210844 12-13 26.676272237630137 23.499038913102655 27.748734458775655 22.075954390491557 14-15 23.27161703244753 24.500996108759317 26.333695681454007 25.89369117733915 16-17 22.919232087119003 28.093579176459883 26.363242120366852 22.623946616054265 18-19 22.62824429677707 26.244496215028924 28.90115372851943 22.22610575967458 20-21 23.44297771878078 25.572140419018847 28.812996354371734 22.171885507828637 22-23 23.119496867975702 25.655455907336528 28.86792282327993 22.357124401407837 24-25 22.988389348986047 25.773850911112888 28.962787868521755 22.27497187137931 26-27 22.974646665127775 25.972467056031235 28.780532061646397 22.27235421719459 28-29 22.480383340888288 26.117424762353693 29.00893605026043 22.393255846497592 30-31 22.70658572775385 25.85689587752566 29.054076675023033 22.382441719697457 32-33 22.521198044369932 26.13464105939336 28.909268218868807 22.434892677367905 34-35 22.988200617126388 26.017877751396988 28.5602811297748 22.433640501701827 36-37 23.29964527610615 25.843473562424172 28.27383104483528 22.583050116634396 38-39 22.863395924258885 25.980389203215974 28.562038928355516 22.594175944169617 40-41 22.984200572394517 25.869353461982627 28.76602118863914 22.380424776983716 42-43 22.95590268555167 26.173680927493503 28.148693392139347 22.721722994815476 44-45 22.751106698967842 26.219611381812314 28.08428115944163 22.94500075977821 46-47 23.08202310251076 26.336413679260044 27.701152715225426 22.880410503003766 48-49 23.323168077614874 26.437215148507825 27.339030028352894 22.900586745524407 50-51 22.886655761474312 26.458965725661304 27.46976655409874 23.18461195876565 52-53 23.239754881711523 26.593026918170164 27.3353407666301 22.831877433488213 54-55 22.90948466033118 26.26191084944037 27.25744178504053 23.571162705187923 56-57 22.96494767569055 26.84045667834414 27.097897737648815 23.096697908316504 58-59 23.337740283829035 26.34951351986467 27.114980568746 23.197765627560297 60-61 23.137490005986784 26.409015692002463 27.54334142227047 22.910152879740284 62-63 23.050284040082094 26.31674080009881 27.63059040471214 23.002384755106956 64-65 22.732752371383686 26.596094671942133 27.50003514152664 23.17111781514754 66-67 22.80352434261671 26.662995825115082 27.198626279599296 23.334853552668903 68-69 22.492608608189123 26.490855359675354 27.67710973066314 23.339426301472386 70-71 22.68015872325127 26.48280844015642 27.42851662583879 23.408516210753522 72-73 22.77197952138435 26.585200885308367 27.027821585585947 23.614998007721336 74-75 22.500940172857852 26.85301121543337 27.0012789029899 23.644769708718876 76-77 22.730928442528754 26.598346374537442 27.092253827841372 23.578471355092432 78-79 22.6203634323891 26.651289929632593 27.155339927748873 23.57300671022944 80-81 22.65216619556044 26.97328760700363 27.037439548809733 23.3371066486262 82-83 22.731535175074104 26.727339465026873 26.98324160799382 23.5578837519052 84-85 22.668618490066827 26.510869015662912 26.689466214023998 24.13104628024626 86-87 22.685414531743962 26.227568348638368 27.174833658095505 23.912183461522172 88-89 22.624871355117477 26.44605555449503 26.9363043310081 23.992768759379395 90-91 22.391120533974686 26.455914825507094 27.248178393802142 23.904786246716082 92-93 21.986338123297706 26.236621338266986 27.610976489586374 24.16606404884893 94-95 22.50161923714004 26.098519277126996 26.95688241089842 24.442979074834543 96-97 22.50352861105696 26.01241160050563 26.83569835684289 24.648361431594516 98-99 22.38477520520402 26.007243361815004 26.742852280576923 24.86512915240406 100-101 22.32243005683364 25.909308066195575 27.01187529565955 24.756386581311236 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3883.0 1 2506.0 2 1265.5 3 1434.0 4 1260.0 5 1201.0 6 1752.5 7 3032.5 8 4709.0 9 5355.5 10 4550.0 11 3743.5 12 3701.0 13 3896.0 14 4180.5 15 4395.5 16 4546.0 17 4840.0 18 5170.5 19 5496.5 20 5990.5 21 6845.0 22 8079.0 23 9496.0 24 11105.0 25 13305.5 26 15946.0 27 18793.5 28 21906.0 29 25557.0 30 30110.0 31 34971.0 32 39406.5 33 43768.0 34 48443.0 35 52599.5 36 57424.5 37 62751.5 38 67134.5 39 69564.0 40 72045.0 41 77476.0 42 82546.5 43 88565.5 44 95419.0 45 99701.5 46 101645.5 47 105456.5 48 114839.5 49 117010.0 50 110299.0 51 106327.0 52 105303.0 53 107302.5 54 108798.0 55 104497.5 56 97787.0 57 90732.0 58 83079.0 59 76089.5 60 67933.5 61 57936.0 62 48964.0 63 40790.0 64 33156.5 65 26290.0 66 19056.5 67 14140.5 68 9702.0 69 5390.0 70 3403.5 71 2218.0 72 1523.0 73 1102.5 74 770.5 75 561.5 76 396.5 77 241.5 78 143.0 79 86.5 80 56.5 81 42.0 82 33.0 83 21.0 84 17.0 85 14.5 86 10.0 87 8.5 88 8.0 89 9.0 90 10.0 91 7.0 92 4.5 93 3.0 94 1.0 95 3.5 96 4.0 97 2.0 98 1.5 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08598670749809438 2 0.09792187646264325 3 0.09514703325800024 4 0.09625028417068963 5 0.09421094157450621 6 0.07779590526752163 7 0.09016568822797844 8 0.08652161703151955 9 0.08217547707244012 10-11 0.08817649340055363 12-13 0.0874744246379331 14-15 0.09299067920138007 16-17 0.09013225638213937 18-19 0.09233875820751816 20-21 0.10231816419048129 22-23 0.11086000080236429 24-25 0.13335963305206008 26-27 0.13895946723010472 28-29 0.13900961499886333 30-31 0.14094866205752954 32-33 0.13680311317348454 34-35 0.13130357453295713 36-37 0.13533211195656533 38-39 0.12791024218029126 40-41 0.12403214806295886 42-43 0.12404886398587839 44-45 0.11520614076144373 46-47 0.13357694005001403 48-49 0.15097821580925128 50-51 0.16097433771513392 52-53 0.17021824308963746 54-55 0.16597239866807525 56-57 0.13601746479626634 58-59 0.1281609810240843 60-61 0.12525241043608498 62-63 0.12174206662298238 64-65 0.10838604421027292 66-67 0.12210981692721218 68-69 0.1191176667246152 70-71 0.12777651479693497 72-73 0.15478944623490554 74-75 0.16739325211623585 76-77 0.17302651814011955 78-79 0.16874724187271828 80-81 0.17652014603030264 82-83 0.17561748619264766 84-85 0.1521483304136188 86-87 0.15430468447023898 88-89 0.17474825820083179 90-91 0.263192206368098 92-93 0.5331209296727691 94-95 0.1733775525214298 96-97 0.18608165394027734 98-99 0.20333248639323875 100-101 0.21088808355286914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2991160.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.18823005688325 #Duplication Level Percentage of deduplicated Percentage of total 1 71.315238398829 30.799789225301012 2 13.211592458692689 11.411705890476075 3 5.581408302021132 7.231534373672603 4 2.8853513170461595 4.984528659020824 5 1.7807307052339885 3.8453303683500715 6 1.1130379497765257 2.884208342219415 7 0.7826966913203911 2.366229933865447 8 0.5547298275388244 1.9166239528929536 9 0.4016462336764838 1.56117509473506 >10 2.128461272394483 16.258030140364994 >50 0.13885454839464467 4.130741894878391 >100 0.09719789944189659 8.082403594311206 >500 0.006036263755785409 1.6464981964302456 >1k 0.002779858308536347 1.833787366247212 >5k 1.58849046202077E-4 0.5570995251631308 >10k+ 7.94245231010385E-5 0.49031344207141137 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14294 0.4778748044237019 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9272 0.3099800746198799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6969 0.23298653365249603 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 3584 0.11981973548723572 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 3402 0.11373513954452454 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.343184583907247E-5 2 0.0 0.0 0.0 0.0 3.343184583907247E-5 3 0.0 0.0 0.0 6.686369167814494E-5 3.343184583907247E-5 4 0.0 0.0 0.0 1.3372738335628987E-4 6.686369167814494E-5 5 0.0 0.0 0.0 1.3372738335628987E-4 6.686369167814494E-5 6 0.0 0.0 0.0 1.3372738335628987E-4 1.002955375172174E-4 7 0.0 0.0 0.0 3.6775030422979714E-4 1.002955375172174E-4 8 0.0 0.0 0.0 4.346139959079421E-4 1.002955375172174E-4 9 0.0 0.0 0.0 7.020687626205218E-4 1.002955375172174E-4 10-11 0.0 0.0 0.0 7.522165313791305E-4 1.3372738335628987E-4 12-13 0.0 0.0 0.0 8.692279918158842E-4 1.3372738335628987E-4 14-15 0.0 0.0 0.0 9.360916834940291E-4 1.8387515211489857E-4 16-17 0.0 0.0 0.0 0.0010363872210112464 2.005910750344348E-4 18-19 1.6715922919536234E-5 0.0 0.0 0.001136682758528464 2.005910750344348E-4 20-21 3.343184583907247E-5 0.0 0.0 0.0014877171398387247 2.1730699795397104E-4 22-23 3.343184583907247E-5 0.0 0.0 0.0019557629815857396 2.6745476671257974E-4 24-25 3.343184583907247E-5 0.0 0.0 0.002808275050482087 3.343184583907247E-4 26-27 3.343184583907247E-5 0.0 0.0 0.00359392342770029 3.343184583907247E-4 28-29 3.343184583907247E-5 0.0 0.0 0.005633266023883711 3.343184583907247E-4 30-31 6.686369167814494E-5 0.0 0.0 0.013623477179422031 3.343184583907247E-4 32-33 6.686369167814494E-5 0.0 0.0 0.030506559328153625 4.011821500688696E-4 34-35 6.686369167814494E-5 0.0 0.0 0.055145829711550035 4.011821500688696E-4 36-37 6.686369167814494E-5 0.0 0.0 0.09168683721365625 4.011821500688696E-4 38-39 6.686369167814494E-5 0.0 0.0 0.14934005536313671 4.011821500688696E-4 40-41 6.686369167814494E-5 0.0 0.0 0.21376322229502936 4.011821500688696E-4 42-43 1.1701146043675363E-4 0.0 0.0 0.27833683253319785 4.011821500688696E-4 44-45 1.3372738335628987E-4 0.0 0.0 0.35608259003196086 4.011821500688696E-4 46-47 1.3372738335628987E-4 0.0 0.0 0.4475354043247436 4.011821500688696E-4 48-49 1.6715922919536235E-4 0.0 0.0 0.5493186589817997 4.011821500688696E-4 50-51 1.6715922919536235E-4 0.0 0.0 0.6633212532930368 4.011821500688696E-4 52-53 1.6715922919536235E-4 0.0 0.0 0.7761537329999064 4.847617646665508E-4 54-55 1.6715922919536235E-4 0.0 0.0 0.8955388544912342 5.850573021837682E-4 56-57 1.6715922919536235E-4 0.0 0.0 1.0386940183741424 6.017732251033044E-4 58-59 1.6715922919536235E-4 0.0 0.0 1.1981472071035986 6.017732251033044E-4 60-61 1.6715922919536235E-4 0.0 0.0 1.377876810334452 6.184891480228406E-4 62-63 1.6715922919536235E-4 0.0 0.0 1.5614845076826382 6.686369167814494E-4 64-65 1.8387515211489857E-4 0.0 0.0 1.7474324342395593 6.686369167814494E-4 66-67 2.3402292087350727E-4 0.0 0.0 1.956715789192153 6.686369167814494E-4 68-69 2.3402292087350727E-4 0.0 0.0 2.1953021570226934 6.686369167814494E-4 70-71 2.3402292087350727E-4 0.0 0.0 2.4635760039583303 6.686369167814494E-4 72-73 2.3402292087350727E-4 0.0 0.0 2.736062263469691 8.357961459768117E-4 74-75 2.3402292087350727E-4 0.0 0.0 3.018995974805761 8.357961459768117E-4 76-77 2.3402292087350727E-4 0.0 0.0 3.329945572954974 8.357961459768117E-4 78-79 2.3402292087350727E-4 0.0 0.0 3.6668382834753075 8.357961459768117E-4 80-81 2.6745476671257974E-4 0.0 0.0 4.040539456264459 8.859439147354204E-4 82-83 2.6745476671257974E-4 0.0 0.0 4.418453041629334 0.001002955375172174 84-85 2.6745476671257974E-4 0.0 0.0 4.795831717460784 0.001002955375172174 86-87 2.6745476671257974E-4 0.0 0.0 5.198300993594458 0.001002955375172174 88-89 2.6745476671257974E-4 0.0 0.0 5.628719292849597 0.001002955375172174 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17100 0.0 32.442364 1 GTACATG 12940 0.0 30.732918 1 GGTATCA 11345 0.0 30.703339 1 TACATGG 13595 0.0 28.624851 2 ACATGGG 13535 0.0 27.875166 3 ATCAACG 20690 0.0 27.043442 3 TCAACGC 20780 0.0 26.902124 4 TATCAAC 20830 0.0 26.81611 2 CAACGCA 21040 0.0 26.524572 5 AACGCAG 21570 0.0 25.978054 6 CATGGGA 8605 0.0 23.658827 4 ACGCAGA 23630 0.0 23.412954 7 CATGGGG 5890 0.0 23.3652 4 CGCAGAG 23680 0.0 23.124619 8 GAGTACT 10605 0.0 22.820465 12-13 AGTACTT 10960 0.0 21.756573 12-13 GCAGAGT 23975 0.0 21.078587 9 ATGGGAG 3640 0.0 20.7292 5 GTACTTT 11780 0.0 20.725851 14-15 AGAGTAC 18420 0.0 19.693869 10-11 >>END_MODULE