FastQCFastQC Report
Fri 27 May 2016
SRR939687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991160
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147390.49275197582208907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105730.3534749060565132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81550.27263670281763597No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG46650.15595956083927304No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG40960.13693684055684083No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32070.10721592960590541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104800.033.138931
GTATCAA161450.032.5289151
GTACATG133350.027.7378621
TACATGG138300.026.601842
ATCAACG199750.026.1657123
TCAACGC202250.025.8655414
TATCAAC203250.025.7164252
ACATGGG134600.025.6890243
CAACGCA205850.025.3446315
AACGCAG208700.025.0092816
CATGGGG59750.022.6756574
ACGCAGA233150.022.1246557
CGCAGAG231400.021.9678758
TCGCGCG2850.021.91225260-61
GAGTACT110150.021.09142512-13
AGTACTT115050.020.86692212-13
CATGGGA83350.020.6576444
GCAGAGT240550.020.468229
ATTCGCG12250.020.14473380-81
CGCGGAA11950.020.04988582-83
Produced by FastQC (version 0.11.5)