FastQCFastQC Report
Fri 27 May 2016
SRR939686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011460
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61120.20295803364481016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40240.13362289387871665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133600.041.6269761
GGTATCA89250.035.5100361
GTACATG106550.034.8597341
TATCAAC186750.029.7772882
ATCAACG202850.027.5079773
TACATGG131800.027.4230212
TCAACGC207300.026.9599234
ACATGGG128500.026.6574523
CAACGCA210200.026.5887685
AACGCAG215650.026.0262536
GTGTAGG16650.025.4603731
ACGCAGA223700.024.8153617
CGCAGAG235350.023.1252868
GAGTACT102050.022.75728812-13
CATGGGG60150.022.0700784
GCAGAGT232500.021.8849819
AGTACTT109300.021.67963412-13
CATGGGA82800.020.7684574
GTCCAAG26050.020.5351491
GTACTTT114700.020.23005714-15
Produced by FastQC (version 0.11.5)