FastQCFastQC Report
Fri 27 May 2016
SRR939686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011460
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147360.48933075651013136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107900.35829796842727446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81410.27033399082172765No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG47230.15683422658776808No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG40500.13448626247733658No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32030.10636037005306395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103800.032.7693561
GTATCAA161100.032.622681
GTACATG132050.028.0096471
TACATGG135250.026.8867822
TCAACGC199200.026.3033074
ATCAACG199500.026.2164733
ACATGGG132350.026.1908153
TATCAAC202950.025.8659132
CAACGCA204250.025.6298795
AACGCAG207750.025.2943616
CATGGGG57750.022.7844124
ACGCAGA229800.022.5186447
CGCAGAG229550.022.2967938
CATGGGA85850.021.2047254
GAGTACT110200.021.06272512-13
GCAGAGT238000.020.9309029
AGTACTT116250.020.51386312-13
GAGTGCA28250.020.0194769
AGAGTAC192300.019.96189110-11
ATTCGCG12950.019.47892880-81
Produced by FastQC (version 0.11.5)