##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3011460 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.487917820592006 31.0 31.0 34.0 30.0 34.0 2 31.690281790227996 31.0 31.0 34.0 30.0 34.0 3 31.73850623949845 31.0 31.0 34.0 30.0 34.0 4 35.55240016470416 37.0 35.0 37.0 33.0 37.0 5 35.30322102900254 37.0 35.0 37.0 33.0 37.0 6 35.3075979757327 37.0 35.0 37.0 32.0 37.0 7 35.20457319705392 37.0 35.0 37.0 32.0 37.0 8 35.165725262829326 37.0 35.0 37.0 32.0 37.0 9 36.7623813698339 39.0 35.0 39.0 32.0 39.0 10-11 36.64354050859052 39.0 35.0 39.0 32.0 39.0 12-13 36.56700570487405 39.0 35.0 39.0 32.0 39.0 14-15 37.63853164245914 39.0 36.0 41.0 32.0 41.0 16-17 37.59598267949765 39.0 36.0 41.0 32.0 41.0 18-19 37.563576305180874 39.0 36.0 41.0 32.0 41.0 20-21 37.50663332735617 39.0 36.0 41.0 32.0 41.0 22-23 37.369282009390794 39.0 36.0 41.0 31.5 41.0 24-25 37.22170375830993 39.0 36.0 40.5 31.0 41.0 26-27 36.942873722380504 39.0 36.0 40.0 31.0 41.0 28-29 36.73047043626679 39.0 35.5 40.0 30.0 41.0 30-31 36.4651755294774 38.0 35.0 40.0 30.0 41.0 32-33 36.27139244751715 38.0 35.0 40.0 30.0 41.0 34-35 36.21199667271025 38.0 35.0 40.0 30.0 41.0 36-37 36.10642445856827 38.0 35.0 40.0 30.0 41.0 38-39 36.13699766890478 38.0 35.0 40.0 30.0 41.0 40-41 35.99419118965551 38.0 35.0 40.0 29.5 41.0 42-43 35.778609378839505 38.0 35.0 40.0 28.5 41.0 44-45 35.53741656870754 38.0 34.0 40.0 27.5 41.0 46-47 35.1898487776693 38.0 34.0 40.0 26.5 41.0 48-49 34.8471228241451 38.0 34.0 40.0 26.0 41.0 50-51 34.579748195227566 38.0 33.0 40.0 24.5 41.0 52-53 34.323434480285314 38.0 33.0 40.0 24.0 41.0 54-55 34.00875638394666 37.0 33.0 40.0 23.5 41.0 56-57 33.20280711017247 36.5 32.0 40.0 20.0 41.0 58-59 30.13973321910303 34.5 26.0 39.0 7.5 40.0 60-61 29.844591991924183 34.0 25.5 38.0 9.5 40.0 62-63 31.598148904518077 35.0 30.5 38.5 15.0 40.0 64-65 31.62758114004503 35.0 31.0 38.5 10.5 40.0 66-67 26.851844620217435 31.5 18.5 36.5 2.0 39.5 68-69 19.46325968135057 19.5 7.5 32.0 2.0 36.5 70-71 17.53913101950549 15.5 7.5 29.0 2.0 35.0 72-73 19.254361671747258 20.0 7.5 30.5 2.0 35.0 74-75 20.68507053057321 23.5 8.0 32.5 2.0 35.5 76-77 22.483314073572288 27.5 11.0 33.0 2.0 35.5 78-79 24.66097822982872 30.5 13.0 34.5 2.0 36.0 80-81 26.244745073818017 32.0 22.0 35.0 2.0 36.0 82-83 27.36536879121755 33.0 26.0 35.0 2.0 36.0 84-85 27.424194576716943 33.0 26.5 35.0 2.0 36.0 86-87 27.144389432368353 33.0 26.0 35.0 2.0 35.5 88-89 26.738913350999184 33.0 25.5 35.0 2.0 35.0 90-91 24.9715405484383 32.0 15.5 34.5 2.0 35.0 92-93 23.394878563885953 31.0 2.0 34.0 2.0 35.0 94-95 19.69432833243676 27.0 2.0 34.0 2.0 35.0 96-97 17.040403990091185 17.5 2.0 33.0 2.0 35.0 98-99 17.313520850351658 20.0 2.0 33.0 2.0 35.0 100-101 16.56129186507541 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30164127869153745 1101 2 0.27248247371344547 1101 3 0.2944501595776643 1101 4 0.18043527915581592 1101 5 0.3192710022816314 1101 6 0.28599800428687416 1101 7 0.3033766960069215 1101 8 0.3797545145988863 1101 9 0.40363110392861756 1101 10-11 0.32472409062248886 1101 12-13 0.30583012420542843 1101 14-15 0.35041229320889755 1101 16-17 0.35675148622266306 1101 18-19 0.41408212208768447 1101 20-21 0.49171370365485956 1101 22-23 0.40300464695236116 1101 24-25 0.35047882604693825 1101 26-27 0.3763580038536105 1101 28-29 0.281279329150145 1101 30-31 0.08779336110271174 1101 32-33 0.24640974092444878 1101 34-35 0.13127683432591652 1101 36-37 0.05157359629900782 1101 38-39 0.021343045738497324 1101 40-41 0.02747379123871241 1101 42-43 0.2042912198589093 1101 44-45 0.2559399564262179 1101 46-47 0.11613733743479315 1101 48-49 -0.038574757035213736 1101 50-51 0.01744712524989467 1101 52-53 -0.028907203708875784 1101 54-55 0.06606937366904475 1101 56-57 -3.6181288912305227 1101 58-59 -10.299913083967073 1101 60-61 -11.800847564368812 1101 62-63 -4.190691827907024 1101 64-65 -1.3924727262919916 1101 66-67 -2.322051559400233 1101 68-69 -4.706163638821824 1101 70-71 -4.513612101529773 1101 72-73 -3.5779129912626537 1101 74-75 -6.44387179721619 1101 76-77 -5.12996686121604 1101 78-79 0.8884012525319065 1101 80-81 1.5517122735171114 1101 82-83 0.21859868301150343 1101 84-85 -0.0583636630369746 1101 86-87 -0.06600133473348535 1101 88-89 -0.15812744590995464 1101 90-91 1.1127766628346798 1101 92-93 0.6615701808288286 1101 94-95 -8.773196796192419 1101 96-97 -9.717524404545038 1101 98-99 -8.906884773351702 1101 100-101 -9.472628237769793 1102 1 0.24140032252247323 1102 2 0.2428154843768624 1102 3 0.28848069526643627 1102 4 0.1625465568946538 1102 5 0.2764609201794386 1102 6 0.24849868478581527 1102 7 0.27419870895681697 1102 8 0.33290078019081903 1102 9 0.2708330113671522 1102 10-11 0.28899603543742813 1102 12-13 0.2618975378331001 1102 14-15 0.35376300620166035 1102 16-17 0.29916086077609094 1102 18-19 0.29325506919250444 1102 20-21 0.27010279040825935 1102 22-23 0.31535718070510654 1102 24-25 0.2829613159585378 1102 26-27 0.010506248846844812 1102 28-29 0.098799763257432 1102 30-31 0.14918728450234653 1102 32-33 -0.021528104854077412 1102 34-35 0.12441006836311885 1102 36-37 -0.10369648522476638 1102 38-39 0.12578496005006912 1102 40-41 -0.008021777862552426 1102 42-43 0.025447910694630593 1102 44-45 -0.1284601795034419 1102 46-47 0.03565993765527509 1102 48-49 0.05363540145298629 1102 50-51 0.21122313306882745 1102 52-53 0.09804274474333141 1102 54-55 -0.11645368035916448 1102 56-57 -3.8030542660153124 1102 58-59 -10.22635152050374 1102 60-61 -8.294710101881318 1102 62-63 -2.4760502871043037 1102 64-65 -0.820198050124425 1102 66-67 -2.2548876429416858 1102 68-69 -5.184952646297106 1102 70-71 -4.7640545853338985 1102 72-73 -3.6927643596609627 1102 74-75 -4.453139980147915 1102 76-77 -4.50911434277452 1102 78-79 1.3247446169867239 1102 80-81 1.7204641555220022 1102 82-83 0.37959845929747615 1102 84-85 0.19416252979261017 1102 86-87 0.16945677502759082 1102 88-89 0.06339393919460434 1102 90-91 1.246620331387465 1102 92-93 0.7014528415456702 1102 94-95 -8.751386220303175 1102 96-97 -9.637835160766501 1102 98-99 -8.667141434160408 1102 100-101 -9.566634913464412 1103 1 0.2659649456690296 1103 2 0.19111311423962007 1103 3 0.2638532091368475 1103 4 0.22215029703935585 1103 5 0.29097709827229323 1103 6 0.3549678940911605 1103 7 0.31853384165854237 1103 8 0.32472970770777465 1103 9 0.30275402301505494 1103 10-11 0.3453876292371163 1103 12-13 0.3138203339785832 1103 14-15 0.4764254157600831 1103 16-17 0.4658199568469499 1103 18-19 0.36337507665493973 1103 20-21 0.42074394213922517 1103 22-23 0.3835938841702813 1103 24-25 0.4651892409123022 1103 26-27 0.33040643865249564 1103 28-29 0.3060379794898722 1103 30-31 0.4703832121977456 1103 32-33 0.35216687989657913 1103 34-35 0.3854945359339297 1103 36-37 0.24500346772938997 1103 38-39 0.371684468502103 1103 40-41 0.4084403137308428 1103 42-43 0.2403779705564162 1103 44-45 0.25959676624783157 1103 46-47 0.0429878350304449 1103 48-49 0.00976515064996164 1103 50-51 -0.08478557446824198 1103 52-53 0.14267738914163175 1103 54-55 0.21110256173226105 1103 56-57 0.5454485810694649 1103 58-59 -8.24233448463534 1103 60-61 -7.58981145323267 1103 62-63 -2.131981054681603 1103 64-65 -0.4060886056709698 1103 66-67 -1.9270150580969378 1103 68-69 -3.220360474969116 1103 70-71 -3.8594004432716673 1103 72-73 -3.1110669678664085 1103 74-75 -3.9106200667771596 1103 76-77 -0.5628738026395474 1103 78-79 3.292024028751431 1103 80-81 2.7160759644347543 1103 82-83 1.16222769342356 1103 84-85 0.6754024412977522 1103 86-87 0.5255828234199207 1103 88-89 0.5559625713772078 1103 90-91 1.8731961137274276 1103 92-93 1.4363627852528822 1103 94-95 -7.249396758978106 1103 96-97 -8.99305456271647 1103 98-99 -8.183591105245059 1103 100-101 -9.403067053888151 1104 1 0.215395512393453 1104 2 0.18590892341862286 1104 3 0.19440109156075636 1104 4 0.1786637570755758 1104 5 0.2671138282304284 1104 6 0.14514386439176974 1104 7 0.19251683587749824 1104 8 0.2824480562688123 1104 9 0.3494114169513267 1104 10-11 0.21551531003661495 1104 12-13 0.3849676319506017 1104 14-15 0.4800963574453405 1104 16-17 0.31824740139673935 1104 18-19 0.38540757385988655 1104 20-21 0.3907667674498825 1104 22-23 0.3801236572815938 1104 24-25 0.302148401748525 1104 26-27 0.393540038439383 1104 28-29 0.3422203700650712 1104 30-31 0.3718549874291597 1104 32-33 0.3300448706894912 1104 34-35 0.26400555800506 1104 36-37 0.2433034393914184 1104 38-39 0.19204392591242936 1104 40-41 0.20209025932893354 1104 42-43 0.19875039146374007 1104 44-45 0.23736675288850506 1104 46-47 0.1478042904451229 1104 48-49 0.07914319966815953 1104 50-51 0.07188426918312985 1104 52-53 0.05918068349317451 1104 54-55 0.02055414953323975 1104 56-57 0.5385701509630465 1104 58-59 -8.327839137910022 1104 60-61 -7.5560960654147955 1104 62-63 -2.329873399381281 1104 64-65 -0.553184213585407 1104 66-67 -1.7441060029908293 1104 68-69 -3.286740796522615 1104 70-71 -3.8001481458719155 1104 72-73 -3.086448268504286 1104 74-75 -3.8603623511774323 1104 76-77 -0.40737511183379027 1104 78-79 3.558780690548499 1104 80-81 3.0345149870696453 1104 82-83 1.412715311429718 1104 84-85 1.1058247770162417 1104 86-87 0.9858925219528949 1104 88-89 0.846839943932558 1104 90-91 2.307420135630128 1104 92-93 2.0008740502209044 1104 94-95 -6.049630709666038 1104 96-97 -7.617817900679505 1104 98-99 -5.469773021021812 1104 100-101 -4.856133627127811 1105 1 0.2567439599774559 1105 2 0.25551128850496596 1105 3 0.2317729315157706 1105 4 0.22835574347251253 1105 5 0.26145592624637715 1105 6 0.2020438125143258 1105 7 0.08849640384121926 1105 8 0.19767874782949235 1105 9 0.21904374727441933 1105 10-11 0.19823563853672965 1105 12-13 0.19319021619477184 1105 14-15 0.3144983083870514 1105 16-17 0.34528898944690667 1105 18-19 0.3066858447285199 1105 20-21 0.18300696417454532 1105 22-23 0.2900643014566384 1105 24-25 0.39376592005824307 1105 26-27 0.26928193654127597 1105 28-29 0.23673528020614754 1105 30-31 0.2681412168817445 1105 32-33 0.24843250035575437 1105 34-35 0.2489225749786641 1105 36-37 0.19801815224312946 1105 38-39 0.14819352024834131 1105 40-41 0.21473123463277233 1105 42-43 0.28167923386906324 1105 44-45 0.2723204956698595 1105 46-47 0.2984402513936715 1105 48-49 0.20339046476863842 1105 50-51 0.1525530924719405 1105 52-53 0.27485681714649246 1105 54-55 0.1838054353839027 1105 56-57 0.6396183675542417 1105 58-59 -7.90364532108849 1105 60-61 -7.348137521236158 1105 62-63 -2.124003722510725 1105 64-65 -0.2514746938464292 1105 66-67 -1.4985751381010388 1105 68-69 -3.302539902973969 1105 70-71 -3.740262709712683 1105 72-73 -2.710214787412708 1105 74-75 -3.4481553249955326 1105 76-77 -0.11758686484786551 1105 78-79 3.890347874418726 1105 80-81 3.1952578028196257 1105 82-83 1.658047137155716 1105 84-85 1.1381321539325882 1105 86-87 1.1173071853530097 1105 88-89 1.002175197665732 1105 90-91 2.394596356474917 1105 92-93 2.0389005222308434 1105 94-95 -5.750677166520372 1105 96-97 -7.369643105718675 1105 98-99 -5.2074495937145535 1105 100-101 -4.589284295797992 1106 1 0.1295419973485643 1106 2 0.17079675449544496 1106 3 0.16785658720364083 1106 4 0.1796208548640763 1106 5 0.22864478643525388 1106 6 0.276609905755862 1106 7 0.2435029699368414 1106 8 0.20631999147834534 1106 9 0.20573959394373986 1106 10-11 0.20278415475382872 1106 12-13 0.18856390052116723 1106 14-15 0.20172940936687667 1106 16-17 0.34879576647873023 1106 18-19 0.2060962051765287 1106 20-21 0.21456884999766856 1106 22-23 0.19222679908397566 1106 24-25 0.20776051009526952 1106 26-27 0.24914124894283418 1106 28-29 0.31170182495228715 1106 30-31 0.42235000384690125 1106 32-33 0.32683427043470914 1106 34-35 0.17020514438976164 1106 36-37 0.35726486309631156 1106 38-39 0.5327833073664152 1106 40-41 0.468041207303294 1106 42-43 0.3762063173028096 1106 44-45 0.3977504752664629 1106 46-47 0.3596016321599791 1106 48-49 0.2557707711229611 1106 50-51 0.18085097126901672 1106 52-53 0.35916346184485093 1106 54-55 0.27016045200591066 1106 56-57 0.838951136804333 1106 58-59 -8.335289201291015 1106 60-61 -7.65586367666965 1106 62-63 -2.1643649797499407 1106 64-65 -0.28306231448021535 1106 66-67 0.5901854139151119 1106 68-69 -2.4056750574393604 1106 70-71 -2.5452895363933443 1106 72-73 -1.0779519924516165 1106 74-75 -0.9255649214320485 1106 76-77 2.303143224292757 1106 78-79 4.572069405321624 1106 80-81 3.620399850049104 1106 82-83 1.9860774631756044 1106 84-85 1.52587482724687 1106 86-87 1.431655666225648 1106 88-89 1.4767872863029687 1106 90-91 2.7424317762617427 1106 92-93 2.4268792715249994 1106 94-95 -5.804063361832412 1106 96-97 -7.256985922552872 1106 98-99 -5.282900934691572 1106 100-101 -4.61942719688766 1107 1 0.08054312195346469 1107 2 0.11347910063351563 1107 3 0.11825701499640573 1107 4 0.001374573197473694 1107 5 -0.0073052976345024945 1107 6 -0.01695990617120202 1107 7 0.03406110125399664 1107 8 0.0949092349790348 1107 9 0.017049374708577147 1107 10-11 -0.007618627509678788 1107 12-13 0.1256877879594427 1107 14-15 0.13352429355060025 1107 16-17 0.11348151413678664 1107 18-19 0.21251870479042623 1107 20-21 0.1184660838684195 1107 22-23 0.2282481883386751 1107 24-25 0.20014332748219488 1107 26-27 0.1793510834789558 1107 28-29 0.1918792040294619 1107 30-31 0.17361953428241605 1107 32-33 0.10750304941441868 1107 34-35 -0.029469553265421666 1107 36-37 -0.0019171270430931031 1107 38-39 0.04685170148219697 1107 40-41 0.03251603042799189 1107 42-43 -0.02382583617800549 1107 44-45 -0.16955268460392148 1107 46-47 0.008357448714122029 1107 48-49 -0.06729981151939057 1107 50-51 -0.042280620542065606 1107 52-53 0.15066865409514918 1107 54-55 0.051985855153908744 1107 56-57 0.5869248875232032 1107 58-59 -8.480248289965118 1107 60-61 -7.934852642388808 1107 62-63 -2.3760912410814328 1107 64-65 -0.5402796636701801 1107 66-67 0.5983343656339848 1107 68-69 -2.329515262500724 1107 70-71 -2.4028319770227675 1107 72-73 -0.878158900196528 1107 74-75 -0.9346817370218652 1107 76-77 1.9504996837822972 1107 78-79 3.9913316698757484 1107 80-81 2.951429329921016 1107 82-83 1.399633347679611 1107 84-85 0.9550897157464782 1107 86-87 0.74152841455928 1107 88-89 0.6151900942585868 1107 90-91 1.992328840066083 1107 92-93 1.8030659856568754 1107 94-95 -6.447395747050287 1107 96-97 -8.023489482683283 1107 98-99 -5.967206623221358 1107 100-101 -5.0335215611225586 1108 1 -0.10518414459770753 1108 2 -0.10086057300260975 1108 3 -0.05930533943730154 1108 4 -0.03914888577516962 1108 5 -0.05371906848269958 1108 6 -0.0660532229337889 1108 7 -0.1105509336538546 1108 8 -0.09459693991171036 1108 9 -0.014462266164152027 1108 10-11 -0.11276272060121784 1108 12-13 -0.11133092021678692 1108 14-15 -0.10892832032894972 1108 16-17 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0.5643149259514111 2311 84-85 0.3508316220053942 2311 86-87 0.2629911899269217 2311 88-89 0.35845495588703713 2311 90-91 1.7645715333291925 2311 92-93 2.2184191977722776 2311 94-95 -1.122963672759738 2311 96-97 -5.985799166308759 2311 98-99 -5.486022296135207 2311 100-101 -6.108439862996143 2312 1 -0.19336169062577468 2312 2 -0.2624262270238056 2312 3 -0.21062733438807157 2312 4 -0.21197420303156633 2312 5 -0.250110854242962 2312 6 -0.22831895420488735 2312 7 -0.19055146544666712 2312 8 -0.2800635321273859 2312 9 -0.3219718104456959 2312 10-11 -0.30765988866453853 2312 12-13 -0.3239813965911864 2312 14-15 -0.45329390549697024 2312 16-17 -0.4374928389424966 2312 18-19 -0.35301550450627417 2312 20-21 -0.29985055666058 2312 22-23 -0.35485008600806367 2312 24-25 -0.3144618726100532 2312 26-27 -0.3540583632088712 2312 28-29 -0.35401393391900626 2312 30-31 -0.31127245341947685 2312 32-33 -0.4429730081340395 2312 34-35 -0.3199711096584963 2312 36-37 -0.30470758201574455 2312 38-39 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2313 2 -0.34538675196321833 2313 3 -0.31926945925081895 2313 4 -0.29428314280701073 2313 5 -0.33391547047583714 2313 6 -0.3306119198520747 2313 7 -0.3670900466444351 2313 8 -0.4884611185290666 2313 9 -0.4653770667234909 2313 10-11 -0.3315221285113168 2313 12-13 -0.3490659069507558 2313 14-15 -0.5192733989544607 2313 16-17 -0.5293814472362257 2313 18-19 -0.4181546184449303 2313 20-21 -0.3731737962164843 2313 22-23 -0.37240180283880164 2313 24-25 -0.36716055209561205 2313 26-27 -0.4971085545002367 2313 28-29 -0.44710625385238245 2313 30-31 -0.5907164430777954 2313 32-33 -0.5360503122518878 2313 34-35 -0.4803439000559351 2313 36-37 -0.5988080199376569 2313 38-39 -0.6271601536389682 2313 40-41 -0.49754041947760896 2313 42-43 -0.42091844525368316 2313 44-45 -0.44688216463413255 2313 46-47 -0.4542197394630989 2313 48-49 -0.5926998653269919 2313 50-51 -0.7369688293597036 2313 52-53 -0.5558744533240159 2313 54-55 -0.7180848959642177 2313 56-57 0.1617860017749706 2313 58-59 3.2617124860394675 2313 60-61 3.2868456639893857 2313 62-63 0.5224492055145262 2313 64-65 -0.12911824567789054 2313 66-67 -1.8947321636707706 2313 68-69 -4.8770212125850065 2313 70-71 -3.661051657677689 2313 72-73 -3.054599842616522 2313 74-75 -3.615823989395178 2313 76-77 0.27345527541929116 2313 78-79 2.7776967136669803 2313 80-81 2.3128845085642062 2313 82-83 0.737439801279816 2313 84-85 0.37358909621125846 2313 86-87 0.24065310628286696 2313 88-89 0.36280983797986366 2313 90-91 2.0251613579466046 2313 92-93 3.408352742595195 2313 94-95 5.492936663553337 2313 96-97 0.8308707312437704 2313 98-99 2.3118983562607127 2313 100-101 3.562739113374043 2314 1 -0.40754845515753146 2314 2 -0.3472609256910033 2314 3 -0.377968112549663 2314 4 -0.26091473178502866 2314 5 -0.25440653976969685 2314 6 -0.328340069026396 2314 7 -0.2933835550474271 2314 8 -0.5355282327315365 2314 9 -0.4323272101934137 2314 10-11 -0.4231227994710238 2314 12-13 -0.564288769530954 2314 14-15 -0.6698039848298336 2314 16-17 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2314 82-83 0.9959676701537035 2314 84-85 0.556139310685122 2314 86-87 0.7852474670427334 2314 88-89 0.9215381622559526 2314 90-91 2.4855685779188015 2314 92-93 4.197004565631641 2314 94-95 6.550780018682083 2314 96-97 3.5848217013729204 2314 98-99 6.0146182990447485 2314 100-101 6.334878822567507 2315 1 -0.4924194003813511 2315 2 -0.40907384173481276 2315 3 -0.475684948997138 2315 4 -0.3190653363963918 2315 5 -0.4481064910959276 2315 6 -0.5291907340902355 2315 7 -0.5378275568280912 2315 8 -0.6099228851990901 2315 9 -0.7583526071617825 2315 10-11 -0.5820587666153543 2315 12-13 -0.5307733281672569 2315 14-15 -0.7221614581588582 2315 16-17 -0.5676803625167466 2315 18-19 -0.6598888203281135 2315 20-21 -0.8862673731263584 2315 22-23 -0.8333846088992942 2315 24-25 -0.5965020346309657 2315 26-27 -0.5878453131855963 2315 28-29 -0.6538431799169047 2315 30-31 -0.6456101487671475 2315 32-33 -0.6375372541149531 2315 34-35 -0.6193397446119278 2315 36-37 -0.513024916010572 2315 38-39 -0.7372817255684581 2315 40-41 -0.6770547466218986 2315 42-43 -0.6617081504101563 2315 44-45 -0.8198478672764935 2315 46-47 -0.6833146793176113 2315 48-49 -0.5959022728542749 2315 50-51 -0.5309268684273718 2315 52-53 -0.43203180340437086 2315 54-55 -0.5395543484123166 2315 56-57 0.15495676097612687 2315 58-59 3.2847107251423147 2315 60-61 3.4288419509046015 2315 62-63 0.7139555667768391 2315 64-65 -0.09864276928774984 2315 66-67 -1.983817504842456 2315 68-69 -4.819051316923712 2315 70-71 -3.6619003383711846 2315 72-73 -2.9807052709961717 2315 74-75 -0.6656298808002568 2315 76-77 1.8110831368771656 2315 78-79 3.5611632386876018 2315 80-81 2.522861160878392 2315 82-83 0.8723188869113372 2315 84-85 0.5763185721949107 2315 86-87 0.5649927698507398 2315 88-89 0.6584629327010987 2315 90-91 2.3599031243433544 2315 92-93 3.903715656495695 2315 94-95 6.486684112302008 2315 96-97 4.1092252744779785 2315 98-99 6.519069870376072 2315 100-101 6.732235455797664 2316 1 -0.4535441341952655 2316 2 -0.36531516548401655 2316 3 -0.44417229132317004 2316 4 -0.28628768350132816 2316 5 -0.4358057338490582 2316 6 -0.4731228924583064 2316 7 -0.33436426348201564 2316 8 -0.3731275168799115 2316 9 -0.5100516508652078 2316 10-11 -0.4608531087989647 2316 12-13 -0.5126356955889975 2316 14-15 -0.6246173197438125 2316 16-17 -0.8189951201651482 2316 18-19 -0.6098483240079631 2316 20-21 -0.6107199435273216 2316 22-23 -0.841867644201443 2316 24-25 -0.6884604263779579 2316 26-27 -0.6355740015681306 2316 28-29 -0.5117586661558775 2316 30-31 -0.4921085898304227 2316 32-33 -0.3774430315327635 2316 34-35 -0.4979420724639567 2316 36-37 -0.34626844818198066 2316 38-39 -0.4489398611247424 2316 40-41 -0.5651030985417904 2316 42-43 -0.4749642604640556 2316 44-45 -0.5878638392997146 2316 46-47 -0.7049440899417192 2316 48-49 -0.6937180454845944 2316 50-51 -0.6576625904716096 2316 52-53 -0.6094528741888539 2316 54-55 -0.3147224681990579 2316 56-57 0.4456190988836042 2316 58-59 3.4085671759830483 2316 60-61 3.3752906295405687 2316 62-63 0.7373481116788483 2316 64-65 0.15392668790712705 2316 66-67 -2.012789704805048 2316 68-69 -2.9965009582052495 2316 70-71 -3.2398295006979367 2316 72-73 -0.6551497764520207 2316 74-75 5.52000825369214 2316 76-77 5.86406596540785 2316 78-79 4.921859157653781 2316 80-81 3.4021707157792207 2316 82-83 1.621960511637429 2316 84-85 1.1145794439983163 2316 86-87 1.08544504167714 2316 88-89 1.0769559376481688 2316 90-91 2.606196938873641 2316 92-93 4.20559041304589 2316 94-95 6.577328861765345 2316 96-97 3.3612372208924235 2316 98-99 5.885080502376056 2316 100-101 6.084385793238397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 17.0 4 62.0 5 154.0 6 398.0 7 837.0 8 1951.0 9 3244.0 10 5123.0 11 8194.0 12 11778.0 13 15793.0 14 19407.0 15 25229.0 16 33542.0 17 43831.0 18 46323.0 19 43682.0 20 45732.0 21 47290.0 22 46801.0 23 47913.0 24 51016.0 25 54108.0 26 67676.0 27 94769.0 28 133891.0 29 176320.0 30 212844.0 31 234328.0 32 245661.0 33 245360.0 34 251969.0 35 266230.0 36 261526.0 37 192631.0 38 71614.0 39 4215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14933472127513 19.102146914348292 14.094163702509688 23.654354661866886 2 16.013425168071986 21.145968148768823 38.41848438141904 24.422122301740153 3 18.674243505969535 25.47649676668245 30.265871215869524 25.583388511478493 4 13.036135296500701 16.826290237957668 36.40061631235348 33.736958153188155 5 13.671999210534972 37.92635181764687 33.70361103828939 14.698037933528774 6 32.375890763948384 36.19772469167779 17.045519449038007 14.380865095335817 7 28.313210203688577 33.18118122106885 21.073200374569147 17.43240820067343 8 26.911392060388046 34.367982010037004 19.710857568280073 19.00976836129488 9 27.095129936974093 15.791841830872732 19.897192723795104 37.21583550835807 10-11 25.29369475271131 26.10632384292004 27.3505044065005 21.249476997868143 12-13 26.323842920045426 23.531841698046797 27.93709695629363 22.207218425614155 14-15 22.889235602577564 24.63641690275168 26.319459675661278 26.154887819009474 16-17 22.657182894675675 28.251114077557066 26.468988464067262 22.6227145637 18-19 22.416274730888844 26.40687192495687 28.875473755674815 22.30137958847947 20-21 23.20983841724612 25.686793116959876 28.800150093310222 22.30321837248378 22-23 22.90347539067429 25.763383873602834 28.89108605128409 22.442054684438776 24-25 22.783838520689486 25.95107617342702 28.89659390786586 22.368491398017635 26-27 22.83962024343378 26.117138373786364 28.732634435408393 22.31060694737146 28-29 22.374148785644575 26.255423912801646 28.96836821539779 22.402059086155994 30-31 22.59355827023943 25.9734398735969 28.984313681863945 22.44868817429973 32-33 22.45277210563002 26.175878016924898 28.906191107304714 22.465158770140366 34-35 22.84213946635377 26.14573649691864 28.51995299883161 22.49217103789598 36-37 23.162114344331147 25.93890661509054 28.35056368878111 22.548415351797203 38-39 22.76771976186884 26.06998841513304 28.581870713416446 22.580421109581675 40-41 22.835592072334595 25.906226382612154 28.796745395170202 22.461436149883045 42-43 22.825022870356445 26.252377395363446 28.193927328351847 22.728672405928265 44-45 22.71701538483526 26.246385701245 28.04313610203976 22.99346281187998 46-47 22.906400789270208 26.434569556431008 27.74197697430136 22.917052679997425 48-49 23.17329684471484 26.521493142183484 27.399047740106656 22.906162272995015 50-51 22.783275867569106 26.457966695554624 27.52004766512657 23.238709771749704 52-53 23.188137819944142 26.570855370662393 27.34720397545774 22.893802833935727 54-55 22.843201842905835 26.231939958053562 27.322228456935132 23.602629742105474 56-57 22.767341784770796 26.768920198714007 27.32874208716168 23.134995929353515 58-59 22.002833467961796 26.16378100265573 28.87846602806347 22.954919501319 60-61 22.252050651282413 26.37217761099771 28.566035893956354 22.80973584376352 62-63 22.989114552893046 26.377616757876837 27.61793674618777 23.015331943042348 64-65 22.687025425535516 26.613926761865482 27.495432329740964 23.203615482858037 66-67 22.27334964121376 26.487923434316745 28.202986950618275 23.035739973851214 68-69 21.31836374820168 25.99622025739381 29.869589359324106 22.815826635080406 70-71 22.193250188507086 26.09942635373244 28.776345947386634 22.93097751037384 72-73 21.58911045659409 26.278000085538384 28.888374606730515 23.244514851137012 74-75 21.81161434666126 26.480163500962856 28.37302718440626 23.335194967969624 76-77 20.908412715670448 26.768543686072476 28.67865416621365 23.644389432043422 78-79 22.09789957582772 26.62729609709411 27.748236956752592 23.526567370325573 80-81 22.272148880619884 27.121409146483355 27.220269577379963 23.386172395516798 82-83 22.605156411959737 26.828912739674465 26.969343219364596 23.5965876290012 84-85 22.580175979956316 26.536960522809405 26.71167861699911 24.17118488023517 86-87 22.527907137373248 26.287118750780365 27.16192316172857 24.02305095011782 88-89 22.455121436114045 26.603441520060038 26.884766857271885 24.05667018655403 90-91 22.24931409638133 26.597584886299085 27.229049330088305 23.92405168723128 92-93 22.051075970385128 26.419995670767698 27.143256556187072 24.385671802660102 94-95 20.8805551132602 26.34982862218945 28.0874906768795 24.682125587670853 96-97 20.436238993757406 26.441332055397766 28.168013641815907 24.954415309028928 98-99 21.35754247259921 26.193939794963207 27.461408977118584 24.987108755318996 100-101 20.510756498872865 26.205557216913387 28.07242251184949 25.211263772364255 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1382.0 1 1071.5 2 826.0 3 980.0 4 1039.5 5 1134.0 6 1537.0 7 2280.0 8 3129.0 9 3704.5 10 3942.5 11 3990.0 12 4066.5 13 4138.0 14 4385.0 15 4735.0 16 5024.5 17 5275.5 18 5549.5 19 5990.0 20 6533.5 21 7307.5 22 8374.5 23 9715.0 24 11410.5 25 13503.5 26 16056.0 27 18899.5 28 22176.5 29 26102.5 30 30634.0 31 35430.0 32 40457.5 33 45744.5 34 50901.5 35 56097.0 36 61261.5 37 66059.0 38 70610.5 39 74517.5 40 78307.0 41 83720.5 42 89382.5 43 95489.0 44 101308.0 45 105269.5 46 108923.0 47 113295.0 48 117868.0 49 118823.0 50 116095.5 51 113145.0 52 111114.0 53 110382.0 54 108406.0 55 103033.0 56 95852.0 57 87443.0 58 79050.5 59 70451.0 60 60265.5 61 49820.5 62 41120.5 63 33592.5 64 26283.0 65 19676.0 66 14072.5 67 9976.0 68 6159.5 69 3112.0 70 1801.5 71 1153.0 72 702.0 73 430.5 74 270.0 75 168.5 76 99.5 77 49.0 78 24.0 79 14.5 80 11.5 81 7.0 82 4.0 83 3.0 84 1.5 85 1.0 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025469373659288187 2 0.013249387340359825 3 6.641296912461065E-5 4 0.0 5 0.06066824729533183 6 0.0 7 0.0 8 9.961945368691598E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 3.154616033419006E-4 16-17 0.0 18-19 6.143199644026486E-4 20-21 0.0 22-23 0.0 24-25 9.961945368691598E-5 26-27 1.4942918053037398E-4 28-29 8.965750831822438E-4 30-31 0.004698717565566204 32-33 0.0052134180762819365 34-35 0.0015108950475848924 36-37 0.08549009450565506 38-39 0.07843371653616518 40-41 0.0848093615721278 42-43 0.08808020030151488 44-45 0.0913344357886208 46-47 0.08648628904252421 48-49 0.07645793070470802 50-51 0.07079622508683496 52-53 0.05957243330477576 54-55 0.04751847940865892 56-57 0.07006568242646423 58-59 0.701702828528355 60-61 0.33324367582501513 62-63 0.0033206484562305328 64-65 0.0025901057958598156 66-67 1.0870640818739083 68-69 2.3416880848492094 70-71 2.3219966395037623 72-73 1.7839685733830102 74-75 1.2706959415034569 76-77 0.7383461842428589 78-79 0.5619201317633308 80-81 0.03837009291174381 82-83 1.826356650926793E-4 84-85 0.0012452431710864497 86-87 0.0014610853207414345 88-89 0.0 90-91 0.3302384889721265 92-93 0.056069149183452544 94-95 0.9392454158448061 96-97 1.6401014790168225 98-99 0.6348747783467156 100-101 0.5619035285210495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3011460.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.52905219987062 #Duplication Level Percentage of deduplicated Percentage of total 1 66.55155033827161 24.976166066273123 2 14.894709490216496 11.179686599204862 3 6.440316151372177 7.250968830855488 4 3.4277006622371555 5.145534283145172 5 2.107693060299185 3.9549861440636582 6 1.3890830303217563 3.127858173293979 7 0.9436928066953494 2.479112762217852 8 0.6914033480462219 2.075816987199358 9 0.5266675830908232 1.7788801696015675 >10 2.703433719967111 18.09361576725415 >50 0.1825658509520085 4.748558343772683 >100 0.1273662112715706 9.35513704281247 >500 0.010274735140937494 2.573255563921533 >1k 0.0032772862087132406 2.141282639472777 >5k 8.857530293819569E-5 0.2706188209132494 >10k+ 1.7715060587639137E-4 0.8485218059982317 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14736 0.48933075651013136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10790 0.35829796842727446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8141 0.27033399082172765 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 4723 0.15683422658776808 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 4050 0.13448626247733658 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3203 0.10636037005306395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.961945368691598E-5 0.0 3 0.0 0.0 0.0 9.961945368691598E-5 0.0 4 0.0 0.0 0.0 1.6603242281152663E-4 0.0 5 0.0 0.0 0.0 2.324453919361373E-4 0.0 6 3.320648456230533E-5 0.0 0.0 2.324453919361373E-4 3.320648456230533E-5 7 3.320648456230533E-5 0.0 0.0 4.316842993099693E-4 3.320648456230533E-5 8 3.320648456230533E-5 0.0 0.0 4.648907838722746E-4 3.320648456230533E-5 9 3.320648456230533E-5 0.0 0.0 7.969556294953278E-4 6.641296912461065E-5 10-11 3.320648456230533E-5 0.0 0.0 0.0010127977791503126 6.641296912461065E-5 12-13 3.320648456230533E-5 0.0 0.0 0.0012950528979299076 6.641296912461065E-5 14-15 3.320648456230533E-5 0.0 0.0 0.0014610853207414345 1.328259382492213E-4 16-17 3.320648456230533E-5 0.0 0.0 0.001627117743552961 1.6603242281152663E-4 18-19 3.320648456230533E-5 0.0 0.0 0.0017599436818021823 1.6603242281152663E-4 20-21 3.320648456230533E-5 0.0 0.0 0.0020255955583006247 1.9923890737383196E-4 22-23 3.320648456230533E-5 0.0 0.0 0.002722931734109037 2.9885836106074797E-4 24-25 3.320648456230533E-5 0.0 0.0 0.0035032841213232116 3.652713301853586E-4 26-27 3.320648456230533E-5 0.0 0.0 0.004250430023975082 3.652713301853586E-4 28-29 3.320648456230533E-5 0.0 0.0 0.006923552031240661 3.652713301853586E-4 30-31 3.320648456230533E-5 0.0 0.0 0.015341395867785062 3.652713301853586E-4 32-33 3.320648456230533E-5 0.0 0.0 0.03222689326771732 3.984778147476639E-4 34-35 3.320648456230533E-5 0.0 0.0 0.05709855020488401 4.316842993099693E-4 36-37 3.320648456230533E-5 0.0 0.0 0.0931607924395476 4.482875415911219E-4 38-39 3.320648456230533E-5 0.0 0.0 0.1490639092001886 4.648907838722746E-4 40-41 3.320648456230533E-5 0.0 0.0 0.21448068378793012 4.648907838722746E-4 42-43 3.320648456230533E-5 0.0 0.0 0.28037895240182503 4.648907838722746E-4 44-45 3.320648456230533E-5 0.0 0.0 0.3600745153513578 4.8149402615342725E-4 46-47 1.4942918053037398E-4 0.0 0.0 0.45345114994056035 5.147005107157326E-4 48-49 1.6603242281152663E-4 0.0 0.0 0.547807375824351 5.479069952780379E-4 50-51 1.6603242281152663E-4 0.0 0.0 0.6460985701287747 5.645102375591906E-4 52-53 1.6603242281152663E-4 0.0 0.0 0.7429618855970194 6.143199644026486E-4 54-55 1.9923890737383196E-4 0.0 0.0 0.8494384783460515 6.641296912461065E-4 56-57 2.324453919361373E-4 0.0 0.0 0.9496224422705266 6.641296912461065E-4 58-59 2.324453919361373E-4 1.6603242281152663E-5 0.0 1.0276742842342252 6.973361758084119E-4 60-61 2.324453919361373E-4 6.641296912461065E-5 0.0 1.0952328770762354 6.973361758084119E-4 62-63 2.324453919361373E-4 6.641296912461065E-5 0.0 1.155668678979631 7.139394180895645E-4 64-65 2.324453919361373E-4 6.641296912461065E-5 0.0 1.2237585755746383 7.305426603707172E-4 66-67 2.324453919361373E-4 6.641296912461065E-5 0.0 1.282251798131139 7.305426603707172E-4 68-69 2.324453919361373E-4 6.641296912461065E-5 0.0 1.352516719464977 7.637491449330225E-4 70-71 2.324453919361373E-4 6.641296912461065E-5 0.0 1.4605706202307185 7.969556294953278E-4 72-73 2.324453919361373E-4 6.641296912461065E-5 0.0 1.6068119782431114 8.799718409010912E-4 74-75 2.324453919361373E-4 6.641296912461065E-5 0.0 1.8054863753793842 8.965750831822438E-4 76-77 2.324453919361373E-4 6.641296912461065E-5 0.0 2.057257941330783 8.965750831822438E-4 78-79 2.324453919361373E-4 6.641296912461065E-5 0.0 2.372271257131093 8.965750831822438E-4 80-81 2.656518764984426E-4 6.641296912461065E-5 0.0 2.7432208961766054 9.463848100257018E-4 82-83 2.656518764984426E-4 6.641296912461065E-5 0.0 3.1172089285595694 0.0010294010214314652 84-85 2.822551187795953E-4 6.641296912461065E-5 0.0 3.448609644491376 0.0010294010214314652 86-87 2.9885836106074797E-4 6.641296912461065E-5 0.0 3.7560684850537616 0.0010294010214314652 88-89 2.9885836106074797E-4 6.641296912461065E-5 0.0 4.053797825639391 0.0010294010214314652 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10380 0.0 32.769356 1 GTATCAA 16110 0.0 32.62268 1 GTACATG 13205 0.0 28.009647 1 TACATGG 13525 0.0 26.886782 2 TCAACGC 19920 0.0 26.303307 4 ATCAACG 19950 0.0 26.216473 3 ACATGGG 13235 0.0 26.190815 3 TATCAAC 20295 0.0 25.865913 2 CAACGCA 20425 0.0 25.629879 5 AACGCAG 20775 0.0 25.294361 6 CATGGGG 5775 0.0 22.784412 4 ACGCAGA 22980 0.0 22.518644 7 CGCAGAG 22955 0.0 22.296793 8 CATGGGA 8585 0.0 21.204725 4 GAGTACT 11020 0.0 21.062725 12-13 GCAGAGT 23800 0.0 20.930902 9 AGTACTT 11625 0.0 20.513863 12-13 GAGTGCA 2825 0.0 20.019476 9 AGAGTAC 19230 0.0 19.961891 10-11 ATTCGCG 1295 0.0 19.478928 80-81 >>END_MODULE