FastQCFastQC Report
Fri 27 May 2016
SRR939685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065578
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48260.4528997407979519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.29580190281706265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24010.22532372102276885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.041.3784181
GTATCAA41450.038.5748561
ATCAACG50300.031.8836543
TCAACGC52250.030.7845484
CAACGCA52600.030.581145
TATCAAC52700.030.4316482
AACGCAG53450.030.0948186
GTACATG41000.028.236111
TACATGG41500.027.6685662
ACGCAGA59750.026.5245697
ACATGGG40900.026.4503153
CGCAGAG60100.026.292388
GCAGAGT63250.024.0099019
GCGTACA1000.001664109823.7240772
GAGTACT31450.022.40923912-13
CATGGGA23950.022.3868124
AGAGTAC51400.021.697310-11
CGTACAC1150.003746728920.6296353
ATAGGAC2551.2067176E-820.4678333
AGTACTT34050.020.34965712-13
Produced by FastQC (version 0.11.5)