FastQCFastQC Report
Fri 27 May 2016
SRR939685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065578
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54790.5141810360198877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46270.4342244303091843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36210.33981557427048986No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12870.12077952059821055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38300.038.7938041
GTATCAA57400.030.7999481
TATCAAC73250.024.0047512
ATCAACG74300.023.6644063
ATCACGC1201.8346273E-423.5062243
TCAACGC75050.023.4279214
CAACGCA75550.023.2728715
GGACCGT1652.6759008E-622.775736
AACGCAG77700.022.7317626
AGTACTT52200.021.64368612-13
GTACATG38700.021.0188621
TACATGG38050.020.8816722
AGAGTAC71700.020.60573610-11
GAGTACT50550.020.58444612-13
ACGCAGA87800.020.0098277
CGCAGAG88400.019.8218048
TAGACAG6250.019.5571775
ACATGGG39300.018.5418053
GCAGAGT91050.018.5225649
GTACTTT56200.018.51458214-15
Produced by FastQC (version 0.11.5)