##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939685_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1065578 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.699507685031033 31.0 30.0 31.0 28.0 34.0 2 30.937265033624943 31.0 31.0 33.0 27.0 34.0 3 30.907679212596356 31.0 31.0 34.0 27.0 34.0 4 34.8064355683019 35.0 35.0 37.0 32.0 37.0 5 34.470562455305945 35.0 35.0 37.0 30.0 37.0 6 34.57403024461841 35.0 35.0 37.0 30.0 37.0 7 34.49858292870161 35.0 35.0 37.0 30.0 37.0 8 34.456128035676414 35.0 35.0 37.0 30.0 37.0 9 35.93352997152719 37.0 35.0 39.0 30.0 39.0 10-11 35.756531666382 37.0 35.0 39.0 30.0 39.0 12-13 35.68003656231642 37.0 35.0 39.0 30.0 39.0 14-15 36.47064410113572 38.0 35.0 40.0 30.0 41.0 16-17 36.377387202063105 38.0 35.0 40.0 30.0 41.0 18-19 36.33266546418939 38.0 34.5 40.0 30.0 41.0 20-21 36.242330453519116 38.0 34.0 40.0 29.5 41.0 22-23 36.04328026667217 38.0 34.0 40.0 29.0 41.0 24-25 35.83236421923125 38.0 34.0 40.0 28.5 41.0 26-27 35.421475011683796 38.0 34.0 40.0 27.0 41.0 28-29 35.09746119007712 37.5 33.0 40.0 27.0 41.0 30-31 34.73073299185982 37.0 33.0 39.0 26.0 40.0 32-33 34.433608332754616 37.0 33.0 39.0 25.0 40.0 34-35 34.31120481091014 37.0 33.0 39.0 25.0 40.0 36-37 34.12258839803374 37.0 33.0 39.0 24.5 40.0 38-39 34.07917440112314 37.0 33.0 39.0 24.0 40.0 40-41 33.85102967591298 37.0 32.0 39.0 24.0 40.0 42-43 33.49646670633215 37.0 31.5 39.0 23.5 40.0 44-45 33.10843645420607 36.0 31.0 39.0 22.5 40.0 46-47 32.59936250560729 36.0 31.0 39.0 19.0 40.0 48-49 32.088023119846696 36.0 30.0 39.0 16.0 40.0 50-51 31.64807785070638 35.0 30.0 39.0 13.5 40.0 52-53 31.220257925745464 35.0 29.5 38.5 9.0 40.0 54-55 30.64660494116808 35.0 28.5 38.0 5.0 40.0 56-57 27.854435808547098 33.0 21.0 37.5 2.0 39.5 58-59 22.426850967268468 26.5 8.0 35.0 2.0 38.5 60-61 22.73122098992284 27.0 10.0 34.5 2.0 38.0 62-63 25.13567096918292 30.5 14.5 35.5 2.0 39.0 64-65 25.096500678505002 31.5 7.5 36.0 2.0 39.0 66-67 18.773971027930383 18.5 2.0 31.5 2.0 36.0 68-69 12.775759259294016 8.0 2.0 22.0 2.0 31.5 70-71 11.681228403739567 8.0 2.0 19.0 2.0 29.5 72-73 12.154939385009826 8.0 2.0 21.5 2.0 30.0 74-75 12.659430844105264 9.0 2.0 22.0 2.0 30.5 76-77 13.994838482025717 11.0 2.0 25.5 2.0 31.5 78-79 16.22315635270248 15.0 2.0 30.0 2.0 34.0 80-81 18.7759873045427 24.0 2.0 32.0 2.0 35.0 82-83 19.800230954467903 26.5 2.0 33.5 2.0 35.0 84-85 19.84197590415718 27.5 2.0 34.0 2.0 35.0 86-87 19.546363569818446 27.0 2.0 34.0 2.0 35.0 88-89 18.96667770918694 26.0 2.0 33.5 2.0 35.0 90-91 17.474953499415342 21.5 2.0 32.5 2.0 34.5 92-93 14.806921689449293 2.0 2.0 31.5 2.0 34.0 94-95 9.687247202926486 2.0 2.0 20.5 2.0 32.5 96-97 7.985607341743166 2.0 2.0 4.5 2.0 31.0 98-99 7.518097689704554 2.0 2.0 2.0 2.0 31.0 100-101 7.2295908887007805 2.0 2.0 2.0 2.0 30.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.47102563401366027 1101 2 0.488054823517615 1101 3 0.34713390811143086 1101 4 0.3761301529168719 1101 5 0.5311052783981935 1101 6 0.49458978046793334 1101 7 0.5129488980647281 1101 8 0.6127909023758846 1101 9 0.58363922388898 1101 10-11 0.8025463437728106 1101 12-13 0.5969075865259228 1101 14-15 0.9085142044821524 1101 16-17 0.7415748640765045 1101 18-19 0.5650326173548734 1101 20-21 0.5428084084703855 1101 22-23 0.5179409321398936 1101 24-25 0.5655112448133153 1101 26-27 0.7522920835290279 1101 28-29 0.7438677675448986 1101 30-31 0.5774203491792349 1101 32-33 0.8787487189020098 1101 34-35 0.3704597589530749 1101 36-37 -0.0026329365885828793 1101 38-39 0.38908502436723325 1101 40-41 0.5965624334227186 1101 42-43 0.6783491783830513 1101 44-45 0.2708147112631707 1101 46-47 0.5055559200461914 1101 48-49 0.35403766225481803 1101 50-51 0.25859095436931767 1101 52-53 0.31282652075550743 1101 54-55 -0.2407934464399446 1101 56-57 -3.339117250522971 1101 58-59 -9.631972709919793 1101 60-61 -10.29296916499872 1101 62-63 -6.802456047861426 1101 64-65 -8.22509856854687 1101 66-67 -9.857123552327536 1101 68-69 -5.8361725733114955 1101 70-71 -5.212197703299627 1101 72-73 -5.58552354063319 1101 74-75 -6.32471601350757 1101 76-77 -7.609453305064261 1101 78-79 -9.699427638361197 1101 80-81 -9.278413565048773 1101 82-83 -7.853245632133737 1101 84-85 -7.2667263975613565 1101 86-87 -6.932457506451149 1101 88-89 -6.9924678276030345 1101 90-91 -10.073598450122565 1101 92-93 -11.684895846075293 1101 94-95 -7.178130577028273 1101 96-97 -5.6963011364801295 1101 98-99 -5.51000126034743 1101 100-101 -5.19768027451176 1102 1 0.27784503045788256 1102 2 0.32435724272879085 1102 3 0.4213242383925433 1102 4 0.3627585777574325 1102 5 0.30694523279304065 1102 6 0.24910576008812768 1102 7 0.2949455489358641 1102 8 0.2828910199516841 1102 9 0.30093355531022326 1102 10-11 0.3842241148206611 1102 12-13 0.45958617704147287 1102 14-15 0.2749498762675202 1102 16-17 0.45009198382567206 1102 18-19 0.4342687309402251 1102 20-21 0.4806036840609309 1102 22-23 0.6043252981212959 1102 24-25 0.3494087044634355 1102 26-27 0.3425849541600172 1102 28-29 0.5713110283137794 1102 30-31 0.40313203906415396 1102 32-33 0.30125379603885705 1102 34-35 -0.06371026278063141 1102 36-37 0.3280971022470567 1102 38-39 0.3095058032178457 1102 40-41 0.23570983072203688 1102 42-43 0.42760809454794213 1102 44-45 0.3954361528137511 1102 46-47 0.2928969254974376 1102 48-49 0.3097632474616461 1102 50-51 0.18656686914467357 1102 52-53 -0.10082117939057156 1102 54-55 -0.16149035027719094 1102 56-57 -4.07900395020587 1102 58-59 -9.403978980629168 1102 60-61 -9.969364932269892 1102 62-63 -6.457367509720548 1102 64-65 -7.427897228895322 1102 66-67 -8.053915158127943 1102 68-69 -4.950816104861003 1102 70-71 -4.566469624060663 1102 72-73 -5.181499241634366 1102 74-75 -5.60689080953137 1102 76-77 -6.888628136717092 1102 78-79 -9.1021764180669 1102 80-81 -7.682924200314385 1102 82-83 -6.372927108600763 1102 84-85 -5.893335939015733 1102 86-87 -5.756129434337989 1102 88-89 -5.677995315400093 1102 90-91 -9.25333978336089 1102 92-93 -10.467535600947564 1102 94-95 -6.886296750826684 1102 96-97 -5.779021056671102 1102 98-99 -5.5009675196555134 1102 100-101 -5.1952895665629235 1103 1 0.1729461399782828 1103 2 0.1714033683551328 1103 3 0.15155531391151555 1103 4 0.27051068047754967 1103 5 0.39007985161909176 1103 6 0.1421332296657951 1103 7 0.1903341047697893 1103 8 0.22289862553508044 1103 9 0.32584407785854097 1103 10-11 0.37049324062595446 1103 12-13 0.36293654837289324 1103 14-15 0.5841431251937905 1103 16-17 0.5488448918428475 1103 18-19 0.6200999764663493 1103 20-21 0.32789363574310926 1103 22-23 0.5219959763603796 1103 24-25 0.6785022978778983 1103 26-27 0.5143190271736202 1103 28-29 0.6341460095235121 1103 30-31 0.5498963107176991 1103 32-33 0.7923105247432289 1103 34-35 0.7423026219724065 1103 36-37 0.6582560365706058 1103 38-39 0.6998852994597939 1103 40-41 0.722239559605363 1103 42-43 0.4011015179552473 1103 44-45 0.5772286098645765 1103 46-47 0.540482712004227 1103 48-49 0.5698165983922152 1103 50-51 0.301785305973592 1103 52-53 0.5711091847826566 1103 54-55 0.39668341493397463 1103 56-57 -3.915702567779242 1103 58-59 -10.383267187501037 1103 60-61 -10.554872381267746 1103 62-63 -7.095515102777096 1103 64-65 -7.852274242137984 1103 66-67 -7.829895495457935 1103 68-69 -4.526511232813759 1103 70-71 -4.3299270327936625 1103 72-73 -5.051139542278603 1103 74-75 -5.466872019266517 1103 76-77 -6.90190882748302 1103 78-79 -8.867365215489949 1103 80-81 -7.236924401638653 1103 82-83 -5.678833738879931 1103 84-85 -5.256269345422318 1103 86-87 -5.091979693822601 1103 88-89 -5.24676936246523 1103 90-91 -7.039661477169048 1103 92-93 -9.088869262364886 1103 94-95 -6.443684232931194 1103 96-97 -5.540522264616699 1103 98-99 -5.389782278961169 1103 100-101 -5.092512969390411 1104 1 0.11505951130083858 1104 2 0.2830622770484794 1104 3 0.25665859710790784 1104 4 0.26676904583667493 1104 5 0.3669943593052878 1104 6 0.1290178374049944 1104 7 0.07349861245598532 1104 8 0.17134399394218036 1104 9 0.26183665670826173 1104 10-11 0.3367636570717565 1104 12-13 0.3236245370423987 1104 14-15 0.5845375808656712 1104 16-17 0.44029193332178096 1104 18-19 0.4588141959203682 1104 20-21 0.39965972042373465 1104 22-23 0.20824738439112167 1104 24-25 0.3698702411591128 1104 26-27 0.6173703126228602 1104 28-29 0.5866177246706101 1104 30-31 0.5634486066360651 1104 32-33 0.6431835038049769 1104 34-35 0.7839803320174639 1104 36-37 0.7417802132867521 1104 38-39 0.4464884252880523 1104 40-41 0.39392302601140017 1104 42-43 0.6358264550079866 1104 44-45 0.5787256778476149 1104 46-47 0.7043785260116593 1104 48-49 0.8287814059061276 1104 50-51 0.8623295926817853 1104 52-53 0.7090351140829512 1104 54-55 0.4790200833675833 1104 56-57 -3.6981007340262586 1104 58-59 -9.366466067702467 1104 60-61 -9.768949996891916 1104 62-63 -6.244023004314339 1104 64-65 -7.169099795411242 1104 66-67 -7.65710838142401 1104 68-69 -4.604078197099474 1104 70-71 -4.163713144640999 1104 72-73 -4.750367610129121 1104 74-75 -5.2115463500377865 1104 76-77 -6.560018256319489 1104 78-79 -8.360449299773013 1104 80-81 -6.656512386995132 1104 82-83 -5.161927016775506 1104 84-85 -4.615993247158338 1104 86-87 -4.484387509221488 1104 88-89 -4.239779939260885 1104 90-91 -5.830849482403609 1104 92-93 -6.7408827463299 1104 94-95 -5.931854704041715 1104 96-97 -5.208013733606893 1104 98-99 -5.217825488939965 1104 100-101 -4.947743596543869 1105 1 0.36953331376140497 1105 2 0.3689653537127242 1105 3 0.4804828862934798 1105 4 0.3092411531184922 1105 5 0.4008691844101122 1105 6 0.33577054788081284 1105 7 0.30478030388659505 1105 8 0.13393293226195624 1105 9 0.534656083967846 1105 10-11 0.5085136416319997 1105 12-13 0.3359601036388469 1105 14-15 0.605298196496669 1105 16-17 0.653048046932831 1105 18-19 0.3453743140717762 1105 20-21 0.538936969463812 1105 22-23 0.6820264191188627 1105 24-25 0.648476648764408 1105 26-27 0.5840489229170274 1105 28-29 0.8958507521193013 1105 30-31 0.750938357832382 1105 32-33 0.9181426683118588 1105 34-35 0.9892811730050113 1105 36-37 0.8757041261681948 1105 38-39 0.7129674368029129 1105 40-41 0.6923604694433294 1105 42-43 0.6352477303531501 1105 44-45 0.7359942359021545 1105 46-47 0.8389210939519032 1105 48-49 0.8503374351841657 1105 50-51 0.8153820857232468 1105 52-53 0.9048410374123108 1105 54-55 0.6025549037446538 1105 56-57 1.3323584443231553 1105 58-59 -7.285162441169938 1105 60-61 -9.045150464556809 1105 62-63 -4.952697165829093 1105 64-65 -6.348098479305126 1105 66-67 -7.084866826970652 1105 68-69 -3.8319035127626275 1105 70-71 -3.565434038570938 1105 72-73 -2.9149045665166007 1105 74-75 -4.408192409066636 1105 76-77 -6.072991112163276 1105 78-79 -7.929585067405748 1105 80-81 -5.637628766553323 1105 82-83 -3.7446939595121123 1105 84-85 -3.1953389528829703 1105 86-87 -2.972852946528622 1105 88-89 -3.0317606363712013 1105 90-91 -5.416647675206766 1105 92-93 -7.102006132342973 1105 94-95 -6.173597096501151 1105 96-97 -5.306737131971769 1105 98-99 -5.092940222300699 1105 100-101 -4.942853453071266 1106 1 0.21353124227023912 1106 2 0.2628280049495011 1106 3 0.18754857456372065 1106 4 0.15436628923004747 1106 5 0.0949077146401649 1106 6 0.22333467944275043 1106 7 0.0987892943560098 1106 8 0.04190483611806428 1106 9 0.28046644364842166 1106 10-11 0.25847501746797974 1106 12-13 0.3349342458425184 1106 14-15 0.30959221475693965 1106 16-17 0.3716209551408056 1106 18-19 0.08012055022423681 1106 20-21 0.2573600159153884 1106 22-23 0.32814719348081667 1106 24-25 0.3944931069841928 1106 26-27 0.26423250202363846 1106 28-29 0.32987851702235815 1106 30-31 0.40410263474477404 1106 32-33 0.41634432723515147 1106 34-35 0.5844038371700719 1106 36-37 0.5068532475766148 1106 38-39 0.5458555107734355 1106 40-41 0.488695561566594 1106 42-43 0.629613237306117 1106 44-45 0.46031750964922225 1106 46-47 0.5547359278556385 1106 48-49 0.5817252230268863 1106 50-51 0.5038930345922701 1106 52-53 0.665487092989089 1106 54-55 0.5048638871690159 1106 56-57 1.2204657861278498 1106 58-59 -7.779357692807912 1106 60-61 -9.612295638266668 1106 62-63 -6.18994408485678 1106 64-65 -7.222074320972009 1106 66-67 -7.753625378360564 1106 68-69 -4.2793863914432 1106 70-71 -4.226212219687986 1106 72-73 -5.059647848693002 1106 74-75 -5.535462428434338 1106 76-77 -6.761899117438411 1106 78-79 -8.749311262251986 1106 80-81 -6.895142247931531 1106 82-83 -5.110463247801636 1106 84-85 -4.826684594037916 1106 86-87 -4.505184152751996 1106 88-89 -4.341894751828406 1106 90-91 -6.325482288390399 1106 92-93 -7.566687578777746 1106 94-95 -6.078671879062558 1106 96-97 -5.1855780733384345 1106 98-99 -4.8467294072875795 1106 100-101 -4.6953535179598616 1107 1 0.0977078701835623 1107 2 -0.04237504073577725 1107 3 -0.09230923887441023 1107 4 -0.003916555592830662 1107 5 0.11496529883316953 1107 6 0.013659989196582956 1107 7 -0.09385302866129308 1107 8 0.10804342025648594 1107 9 0.14131328606968196 1107 10-11 0.13275726188695813 1107 12-13 -0.12472611658311195 1107 14-15 -0.14809431982087773 1107 16-17 -0.0947264058111088 1107 18-19 0.07964909670096176 1107 20-21 -0.039449656045746906 1107 22-23 0.026839268149231543 1107 24-25 0.2336892480245254 1107 26-27 0.20838061075132686 1107 28-29 0.17448920967014203 1107 30-31 -0.24128312476616998 1107 32-33 0.027006272543815157 1107 34-35 -0.08174429884870449 1107 36-37 -0.02350945096604562 1107 38-39 -0.4013908185753934 1107 40-41 -0.48415935901633844 1107 42-43 -0.26406068878961975 1107 44-45 -0.5436704003993569 1107 46-47 -0.6465842784649745 1107 48-49 -0.5837597395843304 1107 50-51 -0.5775333340557331 1107 52-53 -0.7767990813463754 1107 54-55 -0.8203803680572257 1107 56-57 -0.2753272928525554 1107 58-59 -8.650816674304554 1107 60-61 -10.707426908393845 1107 62-63 -7.064746224477979 1107 64-65 -8.046651991404847 1107 66-67 -7.734090418478463 1107 68-69 -3.9176150634589195 1107 70-71 -3.3347290855795872 1107 72-73 -2.991535436706757 1107 74-75 -4.833056036861825 1107 76-77 -6.402302889066597 1107 78-79 -7.124128411970888 1107 80-81 -4.653307741081468 1107 82-83 -3.503902470551484 1107 84-85 -3.2562899649237877 1107 86-87 -2.8478585427962066 1107 88-89 -2.6202228998552606 1107 90-91 -2.295478467638258 1107 92-93 -4.407921681719726 1107 94-95 -5.412576278385739 1107 96-97 -4.9880158496606875 1107 98-99 -4.646786036292546 1107 100-101 -4.767451110886012 1108 1 -0.1312752932438208 1108 2 -0.19132311593720885 1108 3 -0.07520047981356015 1108 4 -0.012311738542557293 1108 5 -0.05570570933964092 1108 6 -0.240920251655929 1108 7 -0.23617549378965919 1108 8 -0.23003308073984385 1108 9 -0.1538906010704082 1108 10-11 -0.15277353777805303 1108 12-13 -0.22746927943291695 1108 14-15 -0.13362800740570435 1108 16-17 -0.26349932867476866 1108 18-19 -0.08409704070626844 1108 20-21 -0.26820322984654155 1108 22-23 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4.2396191929397915 2312 92-93 5.984042293081631 2312 94-95 9.78103265193811 2312 96-97 11.163830125203983 2312 98-99 10.713047687472056 2312 100-101 9.908879257280123 2313 1 -0.3200701995884607 2313 2 -0.2849921486787501 2313 3 -9.699707579926553E-4 2313 4 -0.030826745462093186 2313 5 -0.45510072836609083 2313 6 -0.5575606927559775 2313 7 -0.19106083366753523 2313 8 -0.2950986203453141 2313 9 -0.17637773291723846 2313 10-11 -0.30137814314556977 2313 12-13 -0.6637881033119797 2313 14-15 -0.41060828079584155 2313 16-17 -0.29015049095662704 2313 18-19 -0.19179249714964186 2313 20-21 -0.13937081759886638 2313 22-23 0.15422987980419123 2313 24-25 -0.31029028682863924 2313 26-27 -0.2382383696047583 2313 28-29 -0.5577795308856679 2313 30-31 -0.4792692951886153 2313 32-33 -0.3222110916241121 2313 34-35 -0.5145016735284287 2313 36-37 -0.3901558605317774 2313 38-39 -0.5114854247116583 2313 40-41 -0.6499549703427974 2313 42-43 -0.35449495723453595 2313 44-45 -0.4690489891911582 2313 46-47 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2314 70-71 5.867357630027106 2314 72-73 4.922316296193644 2314 74-75 5.195283590441061 2314 76-77 6.493042808681691 2314 78-79 7.312320730402231 2314 80-81 5.10664143658494 2314 82-83 4.336053059906622 2314 84-85 4.000740457889798 2314 86-87 3.877645268878812 2314 88-89 4.147900203130554 2314 90-91 4.697911099666044 2314 92-93 6.71751066104868 2314 94-95 11.089858076100038 2314 96-97 11.958919810676715 2314 98-99 11.691296302946533 2314 100-101 10.379619546274995 2315 1 -0.3009251400869424 2315 2 -0.3388997044129063 2315 3 -0.32240832754792237 2315 4 -0.28357806289790943 2315 5 -0.07141181674617059 2315 6 -0.5177156798327758 2315 7 -0.4501093573174515 2315 8 -0.28551581596512676 2315 9 -0.5325945686966946 2315 10-11 -0.4646751119015633 2315 12-13 -0.4482560601676653 2315 14-15 -0.7092906166529289 2315 16-17 -0.6291036660454452 2315 18-19 -0.48835140959153023 2315 20-21 -0.30124988749374637 2315 22-23 -0.5024479443489582 2315 24-25 -0.7905024921751007 2315 26-27 -0.5824247559490843 2315 28-29 -0.24364871887028983 2315 30-31 -0.223260920224007 2315 32-33 -0.44531941818380005 2315 34-35 -0.22319880485189003 2315 36-37 -0.20026700918968743 2315 38-39 0.32324031118012897 2315 40-41 -0.12150566308114463 2315 42-43 0.08676384469800524 2315 44-45 0.1376998440675905 2315 46-47 -0.23709671879478833 2315 48-49 -0.23665283954093042 2315 50-51 0.17187900501558318 2315 52-53 0.13036857379610822 2315 54-55 0.5749472755128586 2315 56-57 2.3745208193997236 2315 58-59 6.8484962144557535 2315 60-61 6.663840992662138 2315 62-63 4.031774822824207 2315 64-65 3.3910215945925515 2315 66-67 8.985032972234688 2315 68-69 13.244736665168306 2315 70-71 7.887357924401611 2315 72-73 11.215243458129061 2315 74-75 7.67987431175672 2315 76-77 7.29023472123284 2315 78-79 7.908610630413026 2315 80-81 5.9407614040074925 2315 82-83 4.936423726468622 2315 84-85 4.030437939025298 2315 86-87 3.978090755627054 2315 88-89 4.126865918313406 2315 90-91 4.942988912659732 2315 92-93 7.0259709842718845 2315 94-95 11.080192779910226 2315 96-97 11.713653107376775 2315 98-99 11.04323819341959 2315 100-101 10.12752212793939 2316 1 -0.4443652265096816 2316 2 -0.2686924811372826 2316 3 -0.413820349086091 2316 4 -0.32964205848674766 2316 5 -0.3919284964475196 2316 6 -0.21746276953575716 2316 7 -0.2058132018915515 2316 8 -0.3355788325463607 2316 9 -0.4660459643451773 2316 10-11 -0.5388975323009291 2316 12-13 -0.46129824189173263 2316 14-15 -3.1884705289923616 2316 16-17 -0.879953264596061 2316 18-19 -0.7114237773833096 2316 20-21 -0.400604389522087 2316 22-23 -0.42767412886375666 2316 24-25 -0.7417989739368593 2316 26-27 -0.40699812806762736 2316 28-29 -0.49472548656979143 2316 30-31 -0.10583416293349046 2316 32-33 -0.35431531647954273 2316 34-35 -0.6791357263793643 2316 36-37 -0.3186312787881178 2316 38-39 -0.06475545208007816 2316 40-41 -0.14560247230406986 2316 42-43 -0.04445693276905871 2316 44-45 -0.28623576648993776 2316 46-47 -0.4555771752148843 2316 48-49 -0.48285918508560144 2316 50-51 -0.5861872580448839 2316 52-53 -0.5527684422562373 2316 54-55 0.23603481792245518 2316 56-57 2.203280996296268 2316 58-59 6.759289773260914 2316 60-61 6.026071695240756 2316 62-63 3.2226925580532857 2316 64-65 2.6634853178597346 2316 66-67 8.771685272621227 2316 68-69 14.520182423083357 2316 70-71 14.44434010533797 2316 72-73 13.49999682135776 2316 74-75 12.173915165631396 2316 76-77 10.427264136472724 2316 78-79 8.605904574632099 2316 80-81 5.981803765778938 2316 82-83 5.182042808114478 2316 84-85 4.4841924894413765 2316 86-87 4.1608318081164875 2316 88-89 3.9574575146021793 2316 90-91 4.790776439877831 2316 92-93 7.153386854322692 2316 94-95 11.044904901037098 2316 96-97 11.2212664387596 2316 98-99 10.409133844712837 2316 100-101 9.188767774450127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 12.0 4 42.0 5 116.0 6 267.0 7 659.0 8 1405.0 9 2333.0 10 3629.0 11 5363.0 12 8026.0 13 10927.0 14 15041.0 15 21131.0 16 28055.0 17 36713.0 18 40608.0 19 43725.0 20 47164.0 21 46307.0 22 41045.0 23 36282.0 24 29079.0 25 27707.0 26 38305.0 27 57420.0 28 77652.0 29 88675.0 30 85331.0 31 74003.0 32 59994.0 33 47585.0 34 37671.0 35 27826.0 36 16943.0 37 7078.0 38 1412.0 39 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.477024090561365 22.99224202261218 16.14091077622013 20.389823110606322 2 18.95983279541182 24.03368868375189 35.92317748782051 21.083301033015772 3 21.352844515225083 26.571050238509276 31.146787139737437 20.9293181065282 4 16.45848544170394 19.470090411025755 35.51190058353307 28.559523563737237 5 13.248947050049406 39.27493922759875 34.37379065739394 13.102323064957902 6 31.20090692563097 38.7166401708744 16.38857033459775 13.693882568896882 7 26.78696444558728 34.20566115291419 21.679783178706767 17.32759122279176 8 25.81843673984204 36.39946019399982 19.66603726838644 18.116065797771704 9 26.06472731231313 16.234100178494675 20.28241949439647 37.418753014795726 10-11 24.933510263913107 27.38297900294488 27.676481684118855 20.00702904902316 12-13 25.828001328856264 24.759191725054382 28.41842643147662 20.994380514612725 14-15 22.834336788296575 25.87989415978419 26.718272921919144 24.567496130000087 16-17 21.604706553626297 29.054935443487008 27.422065770877403 21.918292232009296 18-19 21.609315136627792 27.336585091182954 30.25171796514097 20.802381807048288 20-21 22.47278003111926 26.517673976001753 30.292761299501304 20.71678469337768 22-23 22.203385217732414 26.51542736590795 30.193914265926157 21.087273150433475 24-25 22.138595203729807 26.732627738185283 30.227491558571966 20.90128549951294 26-27 21.980631105567934 26.69803941441917 30.215343929495308 21.105985550517584 28-29 21.90572676227381 26.881180545844785 30.27485568363864 20.938237008242762 30-31 22.03916463544546 26.7608740336252 30.192847911353972 21.007113419575372 32-33 22.03152347837512 26.81414849538503 30.01708030087607 21.13724772536378 34-35 22.183222512634497 26.986537279394856 29.873256157704812 20.956984050265827 36-37 22.410426664913548 26.67383566404012 29.979244343848904 20.93649332719742 38-39 22.031952320398506 27.01798691553325 30.035951291072095 20.91410947299615 40-41 22.110631822910186 26.83487412618612 30.013736812073365 21.04075723883033 42-43 22.019381417734646 27.04362462756944 29.604566101962376 21.332427852733538 44-45 22.423940435388232 26.938390602061318 29.42021811678669 21.21745084576376 46-47 22.460316454002026 27.272778503331207 29.020023865776846 21.246881176889925 48-49 22.490468110964855 27.510118900307173 28.734060882930702 21.265352105797277 50-51 22.38486286763791 27.60703640724153 28.65369705354321 21.354403671577348 52-53 22.65320687388487 27.75640905249319 28.25936707672082 21.331016996901116 54-55 22.520608194075244 27.651130668955265 28.29766316835197 21.530597968617514 56-57 23.105716471332645 26.97460500344707 29.112402844541823 20.80727568067846 58-59 25.354373133558404 24.57966254544899 30.947583919417486 19.11838040157512 60-61 23.672933565958218 26.514138759233248 29.549921828975396 20.263005845833145 62-63 22.437736822964936 27.497530589021974 28.74394378424035 21.320788803772743 64-65 22.361816719917254 27.58726666350249 28.58525114556513 21.46566547101512 66-67 26.090780154073638 23.74863665952485 31.852136055751224 18.308447130650286 68-69 28.122327013123684 21.37644029669761 33.78873244209164 16.712500248087068 70-71 27.890545377892867 21.59247598585162 33.671684481068134 16.845294155187386 72-73 27.421707566659588 22.133541212132915 33.084007491359834 17.360743729847663 74-75 26.689395603394285 23.52382605793145 31.40962250270244 18.377155835971827 76-77 23.692989136836562 25.426641098801422 30.837716384591946 20.04265337977007 78-79 23.175722013698984 26.11765453564135 30.30944596054595 20.397177490113723 80-81 22.332328059991482 27.83959199331007 27.95688170876748 21.87119823793097 82-83 22.57842615120041 27.80519392157737 27.80308238059822 21.813297546623996 84-85 22.296094735750525 27.911000122469147 27.71847486965951 22.07443027212081 86-87 22.36652128315999 27.636176796980028 27.878065546924308 22.119236372935674 88-89 22.40801106255628 27.596000850243975 27.669857584134032 22.326130503065713 90-91 22.426662675135493 27.346789688669983 28.42276151825043 21.8037861179441 92-93 22.029868482479266 27.726100262657795 27.9333028392506 22.310728415612342 94-95 24.37566433995787 23.618316787990654 32.670512323626134 19.335506548425347 96-97 25.026254229580474 22.673682567576943 33.62309331668478 18.676969886157806 98-99 23.436108096147354 25.14516335169017 30.62689203547251 20.791836516689973 100-101 22.991674247461816 25.716064993421544 29.87026168683522 21.42199907228142 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 284.0 1 238.5 2 205.0 3 246.0 4 270.5 5 308.5 6 401.0 7 542.5 8 723.5 9 912.0 10 988.5 11 1072.5 12 1231.0 13 1391.0 14 1581.0 15 1744.0 16 1959.0 17 2159.5 18 2288.5 19 2522.5 20 2857.5 21 3165.0 22 3534.5 23 4135.5 24 4881.0 25 5789.5 26 6868.5 27 8189.0 28 9867.5 29 11665.5 30 13592.5 31 15716.5 32 18122.0 33 20516.5 34 23076.0 35 25537.0 36 27892.5 37 30228.5 38 32120.0 39 33723.0 40 34872.5 41 35873.0 42 36918.5 43 37823.0 44 38901.5 45 39642.5 46 40080.0 47 40655.5 48 40715.5 49 40140.0 50 39091.5 51 37986.0 52 36648.0 53 34751.5 54 32620.5 55 30197.5 56 27308.5 57 24038.0 58 20760.0 59 17577.0 60 14502.0 61 11527.0 62 8816.5 63 6577.5 64 4722.0 65 3365.0 66 2333.5 67 1632.0 68 1147.0 69 741.5 70 479.5 71 304.5 72 179.5 73 104.0 74 63.5 75 36.5 76 21.5 77 10.5 78 3.0 79 2.0 80 1.0 81 1.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02092760924118178 2 0.005349209536983684 3 9.384578135059094E-5 4 0.0 5 0.08915349228306141 6 0.0 7 0.0 8 9.384578135059095E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 8.91534922830614E-4 16-17 0.0 18-19 4.692289067529547E-5 20-21 0.0 22-23 3.7538312540236375E-4 24-25 0.0 26-27 3.284602347270683E-4 28-29 0.0034722939099718648 30-31 0.01191841423152505 32-33 0.002158452971063592 34-35 0.0040353685980754105 36-37 0.07582739133127749 38-39 0.07545200820587512 40-41 0.08638504173321897 42-43 0.08915349228306141 44-45 0.08624427306119307 46-47 0.08197429000974119 48-49 0.08094198641488469 50-51 0.08056660328948233 52-53 0.06362743975570066 54-55 0.05527516521549807 56-57 0.629517501299764 58-59 1.7643006893911097 60-61 0.5970468609524596 62-63 0.0038007541446989333 64-65 0.14860479476866076 66-67 2.0389403685136145 68-69 3.066363982739884 70-71 2.9463821512831534 72-73 2.7889089301768615 74-75 2.1585468168449427 76-77 1.6072497743008958 78-79 1.0641173147343508 80-81 0.06508204936663482 82-83 5.161517974282502E-4 84-85 3.284602347270683E-4 86-87 5.161517974282502E-4 88-89 1.4076867202588643E-4 90-91 0.31241260611611726 92-93 0.4939103472481602 94-95 2.176283669520204 96-97 2.5948358543438395 98-99 1.6051382442205073 100-101 1.0678711459883745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1065578.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.63013453428453 #Duplication Level Percentage of deduplicated Percentage of total 1 80.29581580179662 48.683461146030595 2 11.002023996360496 13.341083900975272 3 3.6099348078718867 6.566124991838072 4 1.6360085195063334 3.9676566654761865 5 0.9086883371965834 2.754694806698208 6 0.5590833942851374 2.033838084683539 7 0.365484704700737 1.5511570771360257 8 0.2715033651853316 1.316902844615811 9 0.2026190893503954 1.1056340381875824 >10 1.0556868701220385 11.433590096162135 >50 0.06443025520130811 2.654687362122295 >100 0.02733114047481233 2.8171470535822083 >500 6.176524215072472E-4 0.2729984672091863 >1k 6.176524215072472E-4 0.9880746992200796 >5k 1.544131053768118E-4 0.5129487660628024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5479 0.5141810360198877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4627 0.4342244303091843 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3621 0.33981557427048986 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1287 0.12077952059821055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.384578135059095E-5 0.0 0.0 0.0 0.0 8 9.384578135059095E-5 0.0 0.0 0.0 0.0 9 9.384578135059095E-5 0.0 0.0 0.0 0.0 10-11 9.384578135059095E-5 0.0 0.0 0.0 0.0 12-13 9.384578135059095E-5 0.0 0.0 0.0 0.0 14-15 9.384578135059095E-5 0.0 0.0 0.0 0.0 16-17 9.384578135059095E-5 0.0 0.0 1.4076867202588643E-4 0.0 18-19 1.876915627011819E-4 0.0 0.0 1.876915627011819E-4 0.0 20-21 2.8153734405177286E-4 0.0 0.0 1.876915627011819E-4 0.0 22-23 5.630746881035457E-4 0.0 0.0 3.2846023472706833E-4 0.0 24-25 9.384578135059095E-4 0.0 0.0 5.630746881035457E-4 0.0 26-27 9.384578135059095E-4 0.0 0.0 5.630746881035457E-4 0.0 28-29 0.001079226485531796 0.0 0.0 0.0010323035948565005 0.0 30-31 0.0012199951575576824 0.0 0.0 0.002674604768491842 0.0 32-33 0.0012669180482329778 0.0 0.0 0.007226125163995503 0.0 34-35 0.0015953782829600462 0.0 0.0 0.012809949154355665 0.0 36-37 0.002064607189713001 0.0 0.0 0.023649136900348918 0.0 38-39 0.002158452971063592 0.0 0.0 0.038195233009690514 0.0 40-41 0.002158452971063592 0.0 0.0 0.058043615765340506 0.0 42-43 0.002252298752414183 0.0 0.0 0.08117660086826117 0.0 44-45 0.0024399903151153647 0.0 0.0 0.1088611063666855 0.0 46-47 0.0026276818778165466 0.0 0.0 0.13649868897443454 0.0 48-49 0.0028153734405177286 0.0 0.0 0.1603824403281599 0.0 50-51 0.0028153734405177286 0.0 0.0 0.18332773386837942 0.0 52-53 0.0028153734405177286 0.0 0.0 0.20772763701953306 0.0 54-55 0.0029092192218683195 0.0 0.0 0.2333475353282444 0.0 56-57 0.0031907565659200924 0.0 0.0 0.2525859205051155 0.0 58-59 0.0031907565659200924 0.0 0.0 0.26558356122217236 0.0 60-61 0.0031907565659200924 0.0 0.0 0.2758596742800621 0.0 62-63 0.0031907565659200924 0.0 0.0 0.28505656085242004 0.0 64-65 0.0031907565659200924 0.0 0.0 0.294581907659505 0.0 66-67 0.0031907565659200924 0.0 0.0 0.30349725688781115 0.0 68-69 0.0031907565659200924 0.0 0.0 0.31560336268203737 0.0 70-71 0.0032376794565953876 0.0 0.0 0.3361555887978168 0.0 72-73 0.0032846023472706833 0.0 0.0 0.3697993014120037 0.0 74-75 0.0032846023472706833 0.0 0.0 0.423056782328464 0.0 76-77 0.0032846023472706833 0.0 0.0 0.5172310239137821 0.0 78-79 0.003331525237945979 0.0 0.0 0.6657419729010922 0.0 80-81 0.003472293909971865 0.0 0.0 0.8557796801360389 0.0 82-83 0.003472293909971865 0.0 0.0 1.0407497151780536 0.0 84-85 0.003519216800647161 0.0 0.0 1.1659399874997418 0.0 86-87 0.003566139691322456 0.0 0.0 1.2551404026734785 0.0 88-89 0.003566139691322456 0.0 0.0 1.3458892732395 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3830 0.0 38.793804 1 GTATCAA 5740 0.0 30.799948 1 TATCAAC 7325 0.0 24.004751 2 ATCAACG 7430 0.0 23.664406 3 ATCACGC 120 1.8346273E-4 23.506224 3 TCAACGC 7505 0.0 23.427921 4 CAACGCA 7555 0.0 23.272871 5 GGACCGT 165 2.6759008E-6 22.77573 6 AACGCAG 7770 0.0 22.731762 6 AGTACTT 5220 0.0 21.643686 12-13 GTACATG 3870 0.0 21.018862 1 TACATGG 3805 0.0 20.881672 2 AGAGTAC 7170 0.0 20.605736 10-11 GAGTACT 5055 0.0 20.584446 12-13 ACGCAGA 8780 0.0 20.009827 7 CGCAGAG 8840 0.0 19.821804 8 TAGACAG 625 0.0 19.557177 5 ACATGGG 3930 0.0 18.541805 3 GCAGAGT 9105 0.0 18.522564 9 GTACTTT 5620 0.0 18.514582 14-15 >>END_MODULE