FastQCFastQC Report
Fri 27 May 2016
SRR939684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969499
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.19494604945440894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.12800425786927064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9820.10128942886996274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.057.0328371
GTATCAA29750.054.6049041
TATCAAC42450.034.610942
ATCAACG45100.032.5772553
TCAACGC47500.030.7307454
CAACGCA47750.030.5461735
AACGCAG49050.029.929066
GTATAGG2104.3842672E-529.0088541
ACGCAGA52000.027.5957377
GTACATG29450.027.5805741
TATCGCG350.00859154627.01302790-91
CTTAGCG700.00861657326.9955793
CGCAGAG54650.025.9121118
GCAGAGT56250.024.1693029
TACATGG35800.023.7530942
ACATGGG34650.023.7233873
GCCCTAG3602.89753E-622.5624431
GTCATAA2803.092984E-421.756641
GAGTACT29400.021.67607312-13
AGTACTT32300.021.6298412-13
Produced by FastQC (version 0.11.5)