FastQCFastQC Report
Fri 27 May 2016
SRR939684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969499
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.5297581534380129No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.4528111942353731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35030.361320640867087No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11940.1231563931473885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.037.9271771
GTATCAA54950.030.6998181
TATCAAC70250.023.942812
TCAACGC71050.023.6032564
ATCAACG71500.023.4547043
CAACGCA72550.022.920465
AACGCAG73650.022.5746366
GTACGCT1354.0142873E-420.9364723
GAGTACT47500.020.3272712-13
ACGCAGA83950.019.6926987
AGTACTT49250.019.4615312-13
CGCAGAG83550.019.3923668
AGAGTAC66900.019.36089310-11
GTACATG34850.019.0649991
GTACTTT51100.019.0334714-15
TAGCGGG650.006510654518.75066864-65
GCAGAGT85150.018.0323269
TACATGG36150.017.8552322
GAGTACA31000.017.6326161
CAACCGT2956.978371E-817.5626266
Produced by FastQC (version 0.11.5)