##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939684_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 969499 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.646018201153378 31.0 30.0 31.0 27.0 34.0 2 30.867210796504175 31.0 31.0 33.0 27.0 34.0 3 30.837191167809355 31.0 30.0 34.0 27.0 34.0 4 34.73362427398069 35.0 35.0 37.0 32.0 37.0 5 34.41319691923354 35.0 35.0 37.0 30.0 37.0 6 34.51354978189766 35.0 35.0 37.0 30.0 37.0 7 34.42042333205088 35.0 35.0 37.0 30.0 37.0 8 34.38710509242402 35.0 35.0 37.0 30.0 37.0 9 35.84410917391354 37.0 35.0 39.0 30.0 39.0 10-11 35.672711369480524 37.0 35.0 39.0 30.0 39.0 12-13 35.58613108419916 37.0 35.0 39.0 30.0 39.0 14-15 36.39352696598965 38.0 35.0 40.0 30.0 41.0 16-17 36.28870478463619 38.0 35.0 40.0 30.0 41.0 18-19 36.23006676644329 38.0 34.0 40.0 30.0 41.0 20-21 36.14723945047906 38.0 34.0 40.0 29.0 41.0 22-23 35.945663172422044 38.0 34.0 40.0 29.0 41.0 24-25 35.74106162048646 38.0 34.0 40.0 27.5 41.0 26-27 35.29486621440559 38.0 33.5 40.0 27.0 41.0 28-29 34.96425731228191 37.0 33.0 40.0 27.0 41.0 30-31 34.60343538260483 37.0 33.0 39.0 25.5 40.0 32-33 34.30415657984175 37.0 33.0 39.0 25.0 40.0 34-35 34.14721572688574 37.0 32.5 39.0 25.0 40.0 36-37 33.97544453372309 37.0 32.0 39.0 24.0 40.0 38-39 33.93930267076088 37.0 32.0 39.0 24.0 40.0 40-41 33.69458658544258 37.0 32.0 39.0 24.0 40.0 42-43 33.34766307133891 36.5 31.0 39.0 23.0 40.0 44-45 32.95511547716913 36.0 31.0 39.0 21.5 40.0 46-47 32.459541474514154 36.0 30.5 39.0 18.5 40.0 48-49 31.955591496226404 36.0 30.0 39.0 15.5 40.0 50-51 31.544831402611038 35.0 30.0 39.0 13.5 40.0 52-53 31.133532370843085 35.0 29.0 38.0 9.0 40.0 54-55 30.640644291536145 35.0 28.0 38.0 7.5 40.0 56-57 29.454255239046148 34.0 27.0 38.0 2.0 40.0 58-59 25.751621713895528 31.0 15.0 36.5 2.0 39.0 60-61 25.089961928790025 30.0 15.0 36.0 2.0 39.0 62-63 26.611454472877227 31.5 21.5 36.0 2.0 39.0 64-65 26.466559016564226 32.0 21.0 36.0 2.0 39.0 66-67 21.44676477232055 25.0 5.0 34.0 2.0 37.0 68-69 14.781824426843142 11.0 2.0 26.0 2.0 34.0 70-71 13.144001695721192 9.5 2.0 22.0 2.0 31.0 72-73 14.029430148973852 10.0 2.0 24.5 2.0 32.0 74-75 15.112891297463948 12.0 2.0 27.0 2.0 33.0 76-77 17.263419559999548 19.0 2.0 30.0 2.0 33.5 78-79 19.217919255202943 24.5 2.0 32.5 2.0 35.0 80-81 20.301681590182145 26.5 2.0 33.0 2.0 35.0 82-83 20.92321859022031 28.5 2.0 34.0 2.0 35.0 84-85 20.838633665429256 29.0 2.0 34.0 2.0 35.0 86-87 20.44727843968895 28.5 2.0 34.0 2.0 35.0 88-89 19.803478394510982 27.0 2.0 33.5 2.0 35.0 90-91 18.08741525262017 23.0 2.0 32.5 2.0 34.5 92-93 16.307778553665347 10.0 2.0 32.0 2.0 34.0 94-95 13.13494083026388 2.0 2.0 30.0 2.0 34.0 96-97 11.19812294803811 2.0 2.0 26.0 2.0 33.5 98-99 10.94349194790299 2.0 2.0 26.5 2.0 33.0 100-101 10.204744924956085 2.0 2.0 25.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.200070154072872 1101 2 0.14694538190817497 1101 3 0.2014033260963437 1101 4 0.17958281868701675 1101 5 0.46005957217731464 1101 6 0.1737481094138431 1101 7 0.23626250073520794 1101 8 0.36921088227853716 1101 9 0.48582753269679557 1101 10-11 0.3775346709706824 1101 12-13 0.16051642922469966 1101 14-15 0.5700263474304137 1101 16-17 0.5799320259356477 1101 18-19 0.42035055184761916 1101 20-21 0.4094966839868377 1101 22-23 0.3067307001105277 1101 24-25 0.44869751479475184 1101 26-27 0.34152215208628434 1101 28-29 0.4486410273425392 1101 30-31 0.44750859197586124 1101 32-33 0.45663494664967885 1101 34-35 0.0767083482773856 1101 36-37 0.05012401850655834 1101 38-39 -0.18402450476425258 1101 40-41 -0.20822202200181295 1101 42-43 0.05677257221200449 1101 44-45 0.028254425579973486 1101 46-47 -0.08382817259741415 1101 48-49 -0.16890879122100344 1101 50-51 -0.24007699913878966 1101 52-53 -0.2694297336207505 1101 54-55 -0.18143375095089098 1101 56-57 -4.274937035701328 1101 58-59 -10.708429273161904 1101 60-61 -11.392906311030034 1101 62-63 -5.566607243898744 1101 64-65 -2.5773262646439825 1101 66-67 -2.339205144809508 1101 68-69 -3.156384836495219 1101 70-71 -3.1705988819807356 1101 72-73 -2.7852482161108636 1101 74-75 -5.305513712863563 1101 76-77 -4.816855611204261 1101 78-79 -0.35927600353906897 1101 80-81 0.7492357338297424 1101 82-83 0.2807253704407806 1101 84-85 0.14475695039735825 1101 86-87 0.04575056831223279 1101 88-89 -0.05667273837927311 1101 90-91 0.6788454178296597 1101 92-93 -1.1875988032057574 1101 94-95 -7.115120742610781 1101 96-97 -7.010329205394581 1101 98-99 -6.712900999008516 1101 100-101 -6.71603918393839 1102 1 0.28029365581857135 1102 2 0.42229184673328746 1102 3 0.19165132656797823 1102 4 0.145091363764017 1102 5 0.27510019595592894 1102 6 0.034644147039699646 1102 7 0.2253259594225412 1102 8 0.35582992709024097 1102 9 0.14111823125984557 1102 10-11 0.2489903254382071 1102 12-13 0.1231381313402764 1102 14-15 0.30689894968857345 1102 16-17 0.4000407963485202 1102 18-19 0.2441002870998048 1102 20-21 0.23473583041803892 1102 22-23 0.28935205286816057 1102 24-25 0.410161149907843 1102 26-27 0.367432775406364 1102 28-29 0.17347879424654877 1102 30-31 0.10190023970077533 1102 32-33 -0.027905964257492144 1102 34-35 0.12739581920971688 1102 36-37 -0.2117598832976526 1102 38-39 0.0010205733281622997 1102 40-41 -0.15151194761039477 1102 42-43 0.06373543982936525 1102 44-45 -0.2337271872746598 1102 46-47 -0.2516818374714376 1102 48-49 -0.07908231294560863 1102 50-51 0.0016980776653703344 1102 52-53 -0.5785157149781384 1102 54-55 -0.5791275518124941 1102 56-57 -4.48998022507735 1102 58-59 -10.076469407839394 1102 60-61 -9.050022677794738 1102 62-63 -4.044597693325905 1102 64-65 -1.7758109791421184 1102 66-67 -2.6926784178349266 1102 68-69 -4.060695422259318 1102 70-71 -3.4652992311203334 1102 72-73 -2.778759984014913 1102 74-75 -3.608014842818168 1102 76-77 -4.095660173819031 1102 78-79 -0.06046442101308003 1102 80-81 0.6210440214736082 1102 82-83 -0.061210629367071334 1102 84-85 -0.18776085048171254 1102 86-87 -0.20209951564284978 1102 88-89 -0.19303697023940813 1102 90-91 0.17812841523349476 1102 92-93 -1.1145240384219122 1102 94-95 -7.44296888326229 1102 96-97 -7.27465915868979 1102 98-99 -6.612825777875854 1102 100-101 -6.57926745878702 1103 1 0.07196839569559188 1103 2 0.08898059397528613 1103 3 0.1401467285641722 1103 4 0.1604263233317198 1103 5 0.14630364803307572 1103 6 0.16219755857138551 1103 7 -5.279654273238066E-4 1103 8 0.14352519527815133 1103 9 0.1982942250375359 1103 10-11 0.2505740547038542 1103 12-13 0.1388603289978576 1103 14-15 0.4411885927395076 1103 16-17 0.3366723052191247 1103 18-19 0.19971777866916085 1103 20-21 0.37573824437694014 1103 22-23 0.3006448871142524 1103 24-25 0.4786070693347213 1103 26-27 0.5377515692328529 1103 28-29 0.5051903093217831 1103 30-31 0.3654125857784649 1103 32-33 0.44216767998137385 1103 34-35 0.6218720872833572 1103 36-37 0.5330063303308066 1103 38-39 0.7253975836955462 1103 40-41 0.7310445082806112 1103 42-43 0.6717941874080893 1103 44-45 0.8445945454764114 1103 46-47 0.8143302505395269 1103 48-49 0.754291593444897 1103 50-51 0.8404259715593732 1103 52-53 0.9452410520281838 1103 54-55 1.0067943149676637 1103 56-57 1.1421096406561908 1103 58-59 -7.89976132353355 1103 60-61 -7.7467686082067 1103 62-63 -2.849541478941184 1103 64-65 -0.3520237409418421 1103 66-67 -1.1080755894236312 1103 68-69 -2.6889926731724874 1103 70-71 -2.3235030204782685 1103 72-73 -1.879516961338716 1103 74-75 -2.810731912814143 1103 76-77 -0.14063444166855277 1103 78-79 2.7852528029949077 1103 80-81 2.802818769796449 1103 82-83 1.841856472949349 1103 84-85 1.4696137580710875 1103 86-87 1.2011365030748564 1103 88-89 1.0237531544440834 1103 90-91 1.4999677050780278 1103 92-93 0.02104683001263652 1103 94-95 -6.512093780380608 1103 96-97 -6.913325447778619 1103 98-99 -6.608490808923904 1103 100-101 -6.647437371022253 1104 1 0.12390869523107284 1104 2 0.17240064562171042 1104 3 0.20518517639342093 1104 4 0.12740429582790824 1104 5 0.20672100945070326 1104 6 0.07753427339278574 1104 7 0.19079913184048536 1104 8 0.060493725857618585 1104 9 0.1261459278255117 1104 10-11 0.12176937840089153 1104 12-13 0.03470307315750887 1104 14-15 0.0620758344428225 1104 16-17 0.167875228367663 1104 18-19 0.15318692254273714 1104 20-21 0.30582415146962916 1104 22-23 0.32539390145276315 1104 24-25 0.016876391829846682 1104 26-27 0.1536377519491623 1104 28-29 0.18368759240307497 1104 30-31 0.283249830093375 1104 32-33 0.3785334661486601 1104 34-35 0.3885080844616837 1104 36-37 0.20564388528190136 1104 38-39 0.08456235576079507 1104 40-41 0.18008369998366192 1104 42-43 0.3939455553954545 1104 44-45 0.28295662260624965 1104 46-47 0.01112476286309061 1104 48-49 0.053874714167697846 1104 50-51 0.32078649780417123 1104 52-53 0.17537528795901025 1104 54-55 -0.04770620763812161 1104 56-57 0.22742725927277618 1104 58-59 -8.355655518964578 1104 60-61 -8.522114988795728 1104 62-63 -3.6681479094317453 1104 64-65 -0.9427453501888365 1104 66-67 -1.4810861144343797 1104 68-69 -2.804796777220689 1104 70-71 -2.7831808703156327 1104 72-73 -2.5359276210043955 1104 74-75 -2.8860896007441603 1104 76-77 -0.19428404381660158 1104 78-79 2.6584966495342037 1104 80-81 2.529316002367789 1104 82-83 1.7410670253961804 1104 84-85 1.4194011284025763 1104 86-87 1.313988644955007 1104 88-89 0.9048685174470386 1104 90-91 1.5974272268062037 1104 92-93 -0.07861590127844842 1104 94-95 -6.265300157720928 1104 96-97 -6.447332706287966 1104 98-99 -5.411175412745096 1104 100-101 -4.8392070043257585 1105 1 0.22871411226383742 1105 2 0.20493500761733685 1105 3 0.2958715175666775 1105 4 0.3437805080912071 1105 5 0.37578451129371615 1105 6 0.3032467312200353 1105 7 0.18269983534048606 1105 8 0.2101533283546999 1105 9 0.22924728787820925 1105 10-11 0.28127608879043464 1105 12-13 0.29712903064004337 1105 14-15 0.4371309709151987 1105 16-17 0.4369994542397535 1105 18-19 0.343478409707906 1105 20-21 0.23754923421867602 1105 22-23 0.4542541936139841 1105 24-25 0.5029710621876475 1105 26-27 0.22936274482428587 1105 28-29 0.31561838001797327 1105 30-31 0.49537388213037303 1105 32-33 0.4672798312804076 1105 34-35 0.6663001185570181 1105 36-37 0.6687914358034632 1105 38-39 0.6938230362719793 1105 40-41 0.38084461286626947 1105 42-43 0.5551232113178557 1105 44-45 0.4125302890722793 1105 46-47 0.7204860785227964 1105 48-49 0.5230958501504972 1105 50-51 0.5175873407270153 1105 52-53 0.4048068708399164 1105 54-55 0.3927307333844823 1105 56-57 0.6805680574242139 1105 58-59 -7.503146617306761 1105 60-61 -7.5545866755519775 1105 62-63 -2.7526542354919883 1105 64-65 -0.6475103830815137 1105 66-67 -1.4883206700137173 1105 68-69 -2.363091549514449 1105 70-71 -2.0771339513949236 1105 72-73 -1.5312890620145811 1105 74-75 -2.0676783944113595 1105 76-77 0.5945432669780821 1105 78-79 3.3542207791465977 1105 80-81 3.2365883404735065 1105 82-83 2.2086665873790388 1105 84-85 2.0403696618847533 1105 86-87 2.067826320815225 1105 88-89 1.686563563383256 1105 90-91 2.405353634249657 1105 92-93 0.5159163936168287 1105 94-95 -5.880263102463834 1105 96-97 -6.220451591756653 1105 98-99 -5.382573626382518 1105 100-101 -4.79269576831091 1106 1 0.04087003850453996 1106 2 0.23019247316216251 1106 3 0.12094420247375126 1106 4 0.18059996211252383 1106 5 0.25003129582690775 1106 6 0.3068843687416063 1106 7 0.35140159717082753 1106 8 0.29553666183193883 1106 9 0.2678557095826548 1106 10-11 0.10990191540007999 1106 12-13 0.17475318090198044 1106 14-15 0.23135281376283956 1106 16-17 0.2279663577825346 1106 18-19 0.09101662093758733 1106 20-21 -0.05020004386631882 1106 22-23 0.1413581580097869 1106 24-25 0.17083619205833855 1106 26-27 0.2739250379791329 1106 28-29 0.4395225555916511 1106 30-31 0.295275139206133 1106 32-33 0.201803219353053 1106 34-35 0.3292654160624764 1106 36-37 0.21717916923087444 1106 38-39 0.465598595640472 1106 40-41 0.5557586676664314 1106 42-43 0.49735582522377086 1106 44-45 0.4206317111924207 1106 46-47 0.39572979036861966 1106 48-49 0.054261238689406355 1106 50-51 0.11663987739018111 1106 52-53 0.2890876880686797 1106 54-55 0.25731384828275594 1106 56-57 0.438162504630359 1106 58-59 -8.466441201803196 1106 60-61 -8.436473855747636 1106 62-63 -3.8698462358965813 1106 64-65 -1.3645736661326389 1106 66-67 -0.9692082708381982 1106 68-69 -3.231367193378757 1106 70-71 -2.6615813549062484 1106 72-73 -1.6946774553728385 1106 74-75 -1.6774640587396927 1106 76-77 0.7518205156461946 1106 78-79 2.9751164101576713 1106 80-81 2.5091712358060114 1106 82-83 1.5412381220328086 1106 84-85 1.0556769737952116 1106 86-87 1.0754019569083866 1106 88-89 1.0841749837641395 1106 90-91 1.4220122625062928 1106 92-93 -0.10356902067788987 1106 94-95 -6.151735833751094 1106 96-97 -6.522748952896718 1106 98-99 -5.462063839052944 1106 100-101 -4.8921556735029 1107 1 0.14727059656067 1107 2 0.07239017433007433 1107 3 0.08352803988696422 1107 4 0.04014310391225706 1107 5 0.06676802105328505 1107 6 0.1456310743810718 1107 7 -0.040857317286700834 1107 8 0.14823544084961782 1107 9 0.2745620325435709 1107 10-11 0.21022738113370565 1107 12-13 0.20819567290940455 1107 14-15 0.3110118420027064 1107 16-17 0.21474100375395722 1107 18-19 0.247484758197416 1107 20-21 0.41117594511194255 1107 22-23 0.22085636315706836 1107 24-25 0.33750252367689626 1107 26-27 0.3287087543729683 1107 28-29 0.06477258524341067 1107 30-31 -0.08905084116138795 1107 32-33 0.2483380512895721 1107 34-35 0.22075606520478885 1107 36-37 0.2583016340055906 1107 38-39 0.2272973029578509 1107 40-41 0.1866080213251209 1107 42-43 0.2897610216859263 1107 44-45 0.2278333022688841 1107 46-47 0.16464610406210056 1107 48-49 0.0682756310160002 1107 50-51 0.269284445623736 1107 52-53 0.25869882453176274 1107 54-55 0.014287372111041918 1107 56-57 0.12539576445414014 1107 58-59 -8.573313842645696 1107 60-61 -8.318803619478413 1107 62-63 -3.0843939578039716 1107 64-65 -0.9136521833169198 1107 66-67 -0.1539812723934908 1107 68-69 -2.2901212298708575 1107 70-71 -2.085288555023114 1107 72-73 -1.0405606857458878 1107 74-75 -1.3914958065583605 1107 76-77 1.7771126273405145 1107 78-79 3.7706502858668856 1107 80-81 3.376658703331472 1107 82-83 2.395563224574282 1107 84-85 2.3165373907424787 1107 86-87 2.1241062253542893 1107 88-89 1.7409825247589517 1107 90-91 2.341274379353653 1107 92-93 0.6234880574479593 1107 94-95 -6.46977589612365 1107 96-97 -6.64911770818629 1107 98-99 -5.676289244678938 1107 100-101 -5.049565295950588 1108 1 -0.11077954905164589 1108 2 -0.008345477900160603 1108 3 -0.0652437535812922 1108 4 -0.19123238156087297 1108 5 -0.014537126600849604 1108 6 -0.09110683055505575 1108 7 -0.2173519268268933 1108 8 -0.2553812105330877 1108 9 -0.3602271080238566 1108 10-11 -0.2504347817009105 1108 12-13 -0.1917733642325956 1108 14-15 -0.14970442684652596 1108 16-17 -0.056981376310389464 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2313 92-93 2.729871323998646 2313 94-95 3.18177651855825 2313 96-97 -1.2831465550425456 2313 98-99 -0.15181595277699778 2313 100-101 0.8285417204208549 2314 1 -0.30541546820760246 2314 2 -0.372245979484223 2314 3 -0.5177820473517798 2314 4 -0.20907468420715958 2314 5 -0.5074225637889356 2314 6 -0.4126479465268602 2314 7 -0.5165485064370188 2314 8 -0.6584866866160866 2314 9 -0.5103268764261699 2314 10-11 -0.4044725544820693 2314 12-13 -0.37342357157369577 2314 14-15 -0.676854931594491 2314 16-17 -0.4711468020240588 2314 18-19 -0.3633332980013506 2314 20-21 -0.38870959404007266 2314 22-23 -0.5208664043857496 2314 24-25 -0.6097880675660932 2314 26-27 -0.891500362584182 2314 28-29 -0.40667526918046804 2314 30-31 -0.4372367873271443 2314 32-33 -0.47941879847680013 2314 34-35 -0.38239565566134814 2314 36-37 -0.5567857365199842 2314 38-39 -0.9487182546810828 2314 40-41 -0.5488212821580234 2314 42-43 -0.8382454581535228 2314 44-45 -0.7599586387837789 2314 46-47 -0.8903826429304758 2314 48-49 -0.7234672801299986 2314 50-51 -0.4669402477981528 2314 52-53 -0.5728521764358838 2314 54-55 -0.3351293105575479 2314 56-57 0.46661663623916283 2314 58-59 3.9230876061876714 2314 60-61 4.021855302255222 2314 62-63 1.6134369338234436 2314 64-65 0.5347580722244523 2314 66-67 -1.3686791371715543 2314 68-69 -2.8866246203969634 2314 70-71 -2.454640345168185 2314 72-73 -2.971426710076349 2314 74-75 -4.217238107748528 2314 76-77 -0.18081783392917572 2314 78-79 2.4675958943731366 2314 80-81 2.282146140876886 2314 82-83 1.4694337708612686 2314 84-85 1.4365029018817026 2314 86-87 1.5203417247838686 2314 88-89 1.5215946832119265 2314 90-91 2.449364106869062 2314 92-93 3.418218151991111 2314 94-95 4.6034691630458315 2314 96-97 1.488889035214326 2314 98-99 3.4111292866829572 2314 100-101 3.6885179743163246 2315 1 -0.5547035847995296 2315 2 -0.4527504637891546 2315 3 -0.510343706544603 2315 4 -0.2780432940726314 2315 5 -0.4595806355378187 2315 6 -0.6464596057196843 2315 7 -0.672624739620872 2315 8 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2315 72-73 -3.2276907235292622 2315 74-75 -1.230539677255253 2315 76-77 1.2100397893892136 2315 78-79 2.6752640558081104 2315 80-81 2.0908232709217316 2315 82-83 1.214041394179656 2315 84-85 1.1941486417920189 2315 86-87 1.0948288324071882 2315 88-89 0.8123962527186528 2315 90-91 1.95594482435785 2315 92-93 3.073467591452994 2315 94-95 4.374424880534621 2315 96-97 1.6986452011336102 2315 98-99 3.389971775472196 2315 100-101 3.4029630415808985 2316 1 -0.41882564746271456 2316 2 -0.39660816613902483 2316 3 -0.5957451425759359 2316 4 -0.647547210521715 2316 5 -1.0749037425874235 2316 6 -0.7889139138136798 2316 7 -0.7544654706913718 2316 8 -0.5954810314743568 2316 9 -1.0558665736477764 2316 10-11 -0.8180969968710414 2316 12-13 -0.7898258242636942 2316 14-15 -0.8537859341820351 2316 16-17 -0.8701855450136691 2316 18-19 -1.3130897639475663 2316 20-21 -0.7576051436893962 2316 22-23 -1.2382571360416676 2316 24-25 -1.209874124070673 2316 26-27 -1.0636162988438258 2316 28-29 -0.7834066440941214 2316 30-31 -0.7872672290838665 2316 32-33 -0.38560110947447157 2316 34-35 -0.9911942014820454 2316 36-37 -0.7484839182104395 2316 38-39 -1.2439798781633868 2316 40-41 -1.6867590287655254 2316 42-43 -1.5161204479087012 2316 44-45 -1.4706521839605173 2316 46-47 -1.4810698070466728 2316 48-49 -1.3413720091398247 2316 50-51 -1.199024981257157 2316 52-53 -1.0478123679847826 2316 54-55 -0.6496702254494231 2316 56-57 0.05746625396094984 2316 58-59 3.0854738886637563 2316 60-61 3.309835784405813 2316 62-63 1.1560144139484905 2316 64-65 0.6253325432989314 2316 66-67 -1.312034052929846 2316 68-69 -2.6145967896062974 2316 70-71 -2.441699671468692 2316 72-73 -1.7664717287511937 2316 74-75 3.0522051008126336 2316 76-77 4.185532609232553 2316 78-79 3.4941021759125483 2316 80-81 2.8240869784935363 2316 82-83 1.6122202453545889 2316 84-85 1.101968485134055 2316 86-87 1.211968192720505 2316 88-89 1.075911265773481 2316 90-91 2.0194108817991037 2316 92-93 3.1107505548994734 2316 94-95 4.390226120743497 2316 96-97 1.6311500966949684 2316 98-99 3.6576431201197135 2316 100-101 4.031236540275414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 26.0 4 40.0 5 90.0 6 275.0 7 598.0 8 1407.0 9 2236.0 10 3502.0 11 5231.0 12 7244.0 13 9790.0 14 12931.0 15 17332.0 16 22622.0 17 28925.0 18 30521.0 19 32069.0 20 33732.0 21 33825.0 22 32490.0 23 32101.0 24 29282.0 25 27257.0 26 33239.0 27 46209.0 28 59917.0 29 70927.0 30 72958.0 31 68967.0 32 62475.0 33 56577.0 34 51395.0 35 42085.0 36 27121.0 37 11714.0 38 2320.0 39 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.54916485262409 23.01251431460141 16.15759989270497 20.280720940069536 2 19.25789840690876 23.988380873002445 35.74305059849232 21.010670121596476 3 21.645509691087874 26.544328565578716 31.042115566906208 20.76804617642721 4 16.69965621418898 19.502547191900145 35.315044161984694 28.48275243192618 5 13.094789717914402 39.45958272753257 34.34165408630455 13.103973468248473 6 31.16372476918491 38.762185417416624 16.325029731851192 13.749060081547274 7 26.65624203841365 34.34877189146147 21.66345710516463 17.331528964960253 8 25.887700760908473 36.495344502676126 19.610128530302763 18.006826206112642 9 26.15144523099044 16.102131100702525 20.27975273826997 37.46667093003706 10-11 24.99528106785051 27.429115450351162 27.597759255037914 19.977844226760418 12-13 25.732465943750327 24.854538271829057 28.375996261986863 21.036999522433753 14-15 22.801716357485084 25.964290687419737 26.729792314555517 24.50420064053966 16-17 21.58238430364549 29.05969990685911 27.525505441470283 21.832410348025114 18-19 21.546486339014816 27.359670426186018 30.315798594009557 20.77804464078961 20-21 22.46954354774992 26.4546430682239 30.45676168825342 20.619051695772765 22-23 22.181456608000627 26.443967451229966 30.34118653036259 21.03338941040682 24-25 22.093742280158246 26.761516392237844 30.33944869435074 20.805292633253174 26-27 21.947790532940484 26.73841166334966 30.29112474129666 21.022673062413197 28-29 21.78320560294588 26.949375444821506 30.370916667526227 20.896502284706393 30-31 21.977984571767788 26.845454128163563 30.238695599028944 20.93786570103971 32-33 22.020735276066343 26.809901192314477 30.16919745961407 21.000166072005108 34-35 22.19490534159338 26.872693365350298 30.042311080304408 20.89009021275191 36-37 22.377046388416673 26.649986943161984 30.08058081769867 20.89238585072267 38-39 22.024693269342414 27.01335294345319 30.086103907832435 20.87584987937196 40-41 22.102112888667317 26.868175791759107 30.09012328667442 20.939588032899152 42-43 22.053213297027924 27.04455325974538 29.688651532073102 21.213581911153593 44-45 22.34162026022017 26.956631691947212 29.529260576282905 21.172487471549708 46-47 22.50166826396813 27.31194549253913 29.012929626355323 21.17345661713742 48-49 22.522469057311064 27.434646729033695 28.88362741617194 21.1592567974833 50-51 22.366356237844176 27.578557138117006 28.715379927414663 21.33970669662416 52-53 22.660909614871606 27.73869740855271 28.33812084339295 21.262272133182734 54-55 22.440655019783808 27.713637543268216 28.35113592449906 21.494571512448914 56-57 22.603513893204155 27.647109827484584 28.54091163862289 21.20846464068837 58-59 24.340883579592802 25.852770718096973 29.809861960844692 19.996483741465532 60-61 23.1058943148356 27.02922514940796 29.23120648739092 20.63367404836552 62-63 22.4138375639649 27.526852106857614 28.8214160831046 21.237894246072884 64-65 22.351146392484186 27.714249759663982 28.509266861686104 21.42533698616572 66-67 25.225166437580622 24.908970271713624 30.76264510595469 19.103218184751064 68-69 27.192727095654824 22.120780346729024 33.5328590165531 17.15363354106305 70-71 28.020643722850775 21.73053796581379 33.4714834648201 16.777334846515327 72-73 26.548387266425795 23.17224404790079 32.12424333264964 18.155125353023777 74-75 25.500423873075512 24.727777257705938 30.66215720801264 19.109641661205913 76-77 23.194084547309327 26.42367220698886 29.70861977102312 20.673623474678692 78-79 23.341544625494013 26.319862768196206 29.853104496084466 20.485488110225315 80-81 22.54527480136566 27.667277743408654 28.09831645175787 21.689131003467814 82-83 22.611352788429055 27.909973981366637 27.762526463451426 21.716146766752882 84-85 22.31994306255383 27.910385410810896 27.78485484561391 21.984816681021364 86-87 22.424386206042914 27.66228374416248 27.898028071677416 22.01530197811719 88-89 22.352123288483686 27.687562177765102 27.707056792764504 22.253257740986708 90-91 23.018682007382964 26.674971161116336 29.115447135958583 21.19089969554212 92-93 22.49200499748939 27.607722886013995 27.77157076706095 22.128701349435666 94-95 23.49685301161107 25.49873797268683 30.37690880979513 20.627500205906976 96-97 24.725213267902294 23.65479088061461 32.4777936191609 19.142202232322198 98-99 22.95787454956838 26.232348195651035 29.249402670159164 21.560374584621425 100-101 22.849112426035504 26.119433198380566 29.512353368628673 21.519101006955257 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 396.0 1 317.0 2 247.5 3 273.0 4 279.0 5 319.5 6 430.5 7 633.5 8 858.0 9 1045.0 10 1149.0 11 1202.5 12 1279.5 13 1341.5 14 1479.0 15 1612.0 16 1777.5 17 1954.0 18 2108.0 19 2341.0 20 2574.0 21 2802.5 22 3194.5 23 3665.5 24 4263.5 25 5091.5 26 6190.5 27 7585.5 28 8998.5 29 10589.0 30 12431.0 31 14486.0 32 16574.0 33 18658.0 34 20891.0 35 23101.5 36 25333.0 37 27393.5 38 29032.5 39 30279.0 40 31139.5 41 31941.0 42 32774.0 43 33793.0 44 34773.5 45 35384.0 46 36078.5 47 36525.5 48 36553.5 49 36222.0 50 35230.5 51 34044.0 52 32976.5 53 31713.0 54 29934.0 55 27611.0 56 25094.0 57 22427.0 58 19730.5 59 16864.5 60 13735.0 61 10872.0 62 8473.5 63 6378.0 64 4616.0 65 3314.5 66 2395.5 67 1712.5 68 1155.0 69 765.0 70 514.5 71 321.0 72 188.5 73 118.0 74 71.5 75 38.0 76 18.0 77 7.5 78 4.0 79 3.5 80 2.0 81 2.0 82 2.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021557526103688607 2 0.0064982016484802976 3 0.0 4 0.0 5 0.04084583893330473 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.125842316495427E-4 16-17 0.0 18-19 4.6415726060573554E-4 20-21 0.0 22-23 0.0 24-25 5.157302895619284E-5 26-27 5.157302895619284E-5 28-29 9.283145212114711E-4 30-31 0.004383707461276391 32-33 0.004125842316495427 34-35 0.0013408987528610139 36-37 0.06787010610634978 38-39 0.06307381441342384 40-41 0.06802482519321836 42-43 0.07024246543833465 44-45 0.07261482477031951 46-47 0.0698814542356413 48-49 0.06302224138446764 50-51 0.05884482603901603 52-53 0.04997426505855086 54-55 0.041103704078085695 56-57 0.07385257746526815 58-59 0.8509549777771818 60-61 0.380299515522966 62-63 0.0022692132740724847 64-65 0.0023723593319848707 66-67 1.2075824730092553 68-69 2.562354370659485 70-71 2.4785997716346277 72-73 1.9374955518262527 74-75 1.3857157150239452 76-77 0.7850962198001237 78-79 0.5758128682958931 80-81 0.04584842274205543 82-83 1.547190868685785E-4 84-85 0.0014440448107733994 86-87 0.0017019099555543636 88-89 5.157302895619284E-5 90-91 0.34610659732501015 92-93 0.06317696047133622 94-95 1.0652924861191193 96-97 1.78973882386676 98-99 0.6882420714203934 100-101 0.6394024129988788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 969499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.84152698215038 #Duplication Level Percentage of deduplicated Percentage of total 1 80.99923235483666 50.09116213205099 2 10.7706971845649 13.321527211116827 3 3.4579028906561553 6.415259847425052 4 1.5643963463447461 3.869786354530243 5 0.8278210828849959 2.5596859916812704 6 0.5050676955382851 1.8740494512866024 7 0.3516784277767859 1.5223831686278828 8 0.25388757111345384 1.256063606355622 9 0.19134054940736825 1.0649512574059716 >10 0.9925721247302741 11.084856426753527 >50 0.05960490549731376 2.5189467585016585 >100 0.024300868124545027 2.5736011233512337 >500 8.322214025083611E-4 0.3837293203255716 >1k 4.993328415050167E-4 0.9353402182935809 >5k 1.6644428050167223E-4 0.5286571322940279 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5136 0.5297581534380129 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4390 0.4528111942353731 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3503 0.361320640867087 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1194 0.1231563931473885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.0629211582477136E-4 0.0 0.0 0.0 0.0 3 2.0629211582477136E-4 0.0 0.0 0.0 0.0 4 2.0629211582477136E-4 0.0 0.0 0.0 0.0 5 2.0629211582477136E-4 0.0 0.0 0.0 0.0 6 2.0629211582477136E-4 0.0 0.0 0.0 0.0 7 2.0629211582477136E-4 0.0 0.0 0.0 0.0 8 2.0629211582477136E-4 0.0 0.0 0.0 0.0 9 2.0629211582477136E-4 0.0 0.0 0.0 0.0 10-11 2.0629211582477136E-4 0.0 0.0 0.0 0.0 12-13 5.157302895619284E-4 0.0 0.0 0.0 0.0 14-15 5.157302895619284E-4 0.0 0.0 0.0 0.0 16-17 5.673033185181213E-4 0.0 0.0 0.0 0.0 18-19 6.704493764305068E-4 0.0 0.0 0.0 0.0 20-21 7.220224053866997E-4 0.0 0.0 0.0 0.0 22-23 7.220224053866997E-4 0.0 0.0 5.157302895619284E-5 0.0 24-25 0.0010314605791238568 0.0 0.0 1.0314605791238568E-4 0.0 26-27 0.0010314605791238568 0.0 0.0 2.578651447809642E-4 0.0 28-29 0.0010314605791238568 0.0 0.0 0.0011861796659924353 0.0 30-31 0.0010314605791238568 0.0 0.0 0.0028365165925906062 0.0 32-33 0.0010314605791238568 5.157302895619284E-5 0.0 0.007220224053866997 0.0 34-35 0.0011861796659924351 1.0314605791238568E-4 0.0 0.012429099978442474 0.0 36-37 0.0013408987528610139 2.0629211582477136E-4 0.0 0.021351233987863837 0.0 38-39 0.0014440448107733994 3.09438173737157E-4 0.0 0.03620426632724737 0.0 40-41 0.0014440448107733994 3.09438173737157E-4 0.0 0.05595673641746923 0.0 42-43 0.0015471908686857851 3.09438173737157E-4 0.0 0.07426516169691769 0.0 44-45 0.0016503369265981709 3.09438173737157E-4 0.0 0.09922650771171501 0.0 46-47 0.0018566290424229422 3.09438173737157E-4 0.0 0.13063448234603645 0.0 48-49 0.0020629211582477137 3.09438173737157E-4 0.0 0.16116571548810263 0.0 50-51 0.0020629211582477137 3.09438173737157E-4 0.0 0.1902013307904392 0.0 52-53 0.0020629211582477137 3.09438173737157E-4 0.0 0.22212503571432254 0.0 54-55 0.0020629211582477137 3.09438173737157E-4 0.0 0.2569884032887089 0.0 56-57 0.0020629211582477137 3.610112026933499E-4 0.0 0.28798379369138083 0.0 58-59 0.0020629211582477137 4.125842316495427E-4 5.157302895619284E-5 0.30649851108665405 0.0 60-61 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.3226408691499424 0.0 62-63 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.3354825533600344 0.0 64-65 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.34827266454117023 0.0 66-67 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.3643118765465462 0.0 68-69 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.38581782962127864 5.157302895619284E-5 70-71 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.4208359162825336 1.0314605791238568E-4 72-73 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.47421400125219315 1.0314605791238568E-4 74-75 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.5552352297423722 1.0314605791238568E-4 76-77 0.0020629211582477137 4.125842316495427E-4 1.0314605791238568E-4 0.6718934212412804 1.0314605791238568E-4 78-79 0.0021144941872039066 4.125842316495427E-4 1.0314605791238568E-4 0.8246011599805673 1.0314605791238568E-4 80-81 0.0023723593319848702 4.125842316495427E-4 1.0314605791238568E-4 1.0062413679642785 1.0314605791238568E-4 82-83 0.002475505389897256 4.125842316495427E-4 1.0314605791238568E-4 1.1825695539655017 1.0314605791238568E-4 84-85 0.002475505389897256 4.125842316495427E-4 1.0314605791238568E-4 1.336154034197044 1.0314605791238568E-4 86-87 0.002475505389897256 4.125842316495427E-4 1.0314605791238568E-4 1.4678715501511606 1.0314605791238568E-4 88-89 0.002475505389897256 4.125842316495427E-4 1.0314605791238568E-4 1.6082533349699175 1.0314605791238568E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3690 0.0 37.927177 1 GTATCAA 5495 0.0 30.699818 1 TATCAAC 7025 0.0 23.94281 2 TCAACGC 7105 0.0 23.603256 4 ATCAACG 7150 0.0 23.454704 3 CAACGCA 7255 0.0 22.92046 5 AACGCAG 7365 0.0 22.574636 6 GTACGCT 135 4.0142873E-4 20.936472 3 GAGTACT 4750 0.0 20.32727 12-13 ACGCAGA 8395 0.0 19.692698 7 AGTACTT 4925 0.0 19.46153 12-13 CGCAGAG 8355 0.0 19.392366 8 AGAGTAC 6690 0.0 19.360893 10-11 GTACATG 3485 0.0 19.064999 1 GTACTTT 5110 0.0 19.03347 14-15 TAGCGGG 65 0.0065106545 18.750668 64-65 GCAGAGT 8515 0.0 18.032326 9 TACATGG 3615 0.0 17.855232 2 GAGTACA 3100 0.0 17.632616 1 CAACCGT 295 6.978371E-8 17.562626 6 >>END_MODULE