FastQCFastQC Report
Fri 27 May 2016
SRR939683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552464
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139050.5447677224830595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89150.34927035209899143No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA87640.34335449980881216No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA70990.2781234132978957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66140.25912216587579684No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49650.19451792464066095No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT34420.13485008995229708No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA31880.12489892119928038No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG30190.11827786797384801No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT28440.11142174776999791No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26490.10378207097142213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG147400.028.2361071
TACATGG150650.026.9034822
ACATGGG149750.025.2904073
CATGGGA105600.024.8067234
CCTATAC8250.023.0104873
ATGGGAG43400.021.9786265
GAGTACT105900.021.61965212-13
CGGGTCG3401.8189894E-1220.9333346
AGTACTT110800.020.8776812-13
AGTACAT103300.020.6284122
GTACTTT118650.019.53772514-15
ATGGGAT45500.019.2954335
GTATCAA303700.018.7985171
CATGGGG46300.018.6545414
TGGACGC8150.018.0508775
TACTTTT133800.017.23682814-15
ACTTTTT140800.017.1562416-17
ATAGCCC8900.017.0639573
TATCAAC339800.016.6623732
ATCAACG339500.016.6351593
Produced by FastQC (version 0.11.5)