FastQCFastQC Report
Fri 27 May 2016
SRR939683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552464
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144330.5654536165838187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105130.41187652401757674No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA89410.35028897567213485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79340.31083690112769463No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA78580.3078593860677369No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55940.21916077954478494No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT49850.19530148123538668No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA36880.14448783606742346No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG36140.1415886766669383No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT32770.12838574804580985No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28590.11200941521604221No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC26930.10550589547981871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG142800.026.8974481
TACATGG143350.026.720712
ACATGGG139500.025.7352873
CATGGGA99150.023.205714
GAGTACT108400.021.4806912-13
AGTACTT112600.020.97159812-13
CATGGGG41450.020.6597734
ATGGGAT41450.019.6381365
GTATCAA277450.019.5780811
GTACTTT121050.019.29414214-15
AGTACAT103900.018.7049182
ATGGGAG41600.018.323165
ATCAACG309000.017.4351483
TCAACGC310150.017.4311854
ACTTTTT137450.017.231716-17
AACGCAG313100.017.2302936
CAACGCA313550.017.2121565
TACTTTT134750.017.17557514-15
TATCAAC319350.016.9012092
ATGGGAC19150.016.2163775
Produced by FastQC (version 0.11.5)