##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939683_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2552464 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59167142024334 31.0 31.0 34.0 30.0 34.0 2 31.798929583335944 31.0 31.0 34.0 30.0 34.0 3 31.844430714791667 31.0 31.0 34.0 30.0 34.0 4 35.61736815876737 37.0 35.0 37.0 33.0 37.0 5 35.37169104050047 37.0 35.0 37.0 33.0 37.0 6 35.40299686890785 37.0 35.0 37.0 33.0 37.0 7 35.33456769615556 37.0 35.0 37.0 32.0 37.0 8 35.30338331902037 37.0 35.0 37.0 32.0 37.0 9 36.90878696036457 39.0 37.0 39.0 33.0 39.0 10-11 36.79752858414457 39.0 37.0 39.0 32.0 39.0 12-13 36.744027731635 39.0 35.0 39.0 32.0 39.0 14-15 37.7708808038037 40.0 37.0 41.0 32.0 41.0 16-17 37.753531489572424 39.0 37.0 41.0 32.0 41.0 18-19 37.78299929009773 39.5 37.0 41.0 32.0 41.0 20-21 37.73673869641256 39.5 37.0 41.0 32.0 41.0 22-23 37.60314739012969 39.0 36.5 41.0 32.0 41.0 24-25 37.47721965912154 39.0 36.0 41.0 32.0 41.0 26-27 37.20735160221653 39.0 36.0 41.0 31.0 41.0 28-29 37.01307168289151 39.0 36.0 40.0 31.0 41.0 30-31 36.774881056108924 38.5 35.5 40.0 30.5 41.0 32-33 36.586822380256876 38.0 35.0 40.0 30.0 41.0 34-35 36.55204774680466 38.0 35.0 40.0 30.0 41.0 36-37 36.41854067285571 38.0 35.0 40.0 30.0 41.0 38-39 36.41193137297921 39.0 35.0 40.0 30.0 41.0 40-41 36.296509764682284 39.0 35.0 40.0 30.0 41.0 42-43 36.11259943333187 38.5 35.0 40.0 30.0 41.0 44-45 35.88078029699929 38.0 35.0 40.0 29.0 41.0 46-47 35.53004116806349 38.0 35.0 40.0 27.5 41.0 48-49 35.202629106620115 38.0 34.0 40.0 26.5 41.0 50-51 34.95372686940932 38.0 34.0 40.0 26.0 41.0 52-53 34.7041697747745 38.0 34.0 40.0 25.5 41.0 54-55 34.43768335224317 38.0 33.5 40.0 24.0 41.0 56-57 32.52444833697948 37.0 30.5 39.5 15.5 41.0 58-59 27.6666791382758 33.0 18.0 38.5 6.5 40.0 60-61 28.428977646697465 33.5 20.5 38.0 8.5 40.0 62-63 31.41866956791555 35.0 29.5 38.5 10.0 40.0 64-65 31.629045502698567 35.5 31.0 39.0 5.0 40.0 66-67 25.014746143334442 28.0 18.0 35.5 2.0 39.0 68-69 17.554533188323127 13.0 7.5 28.5 2.0 35.5 70-71 16.44339195381404 13.0 7.5 27.0 2.0 34.0 72-73 17.607987810993613 14.5 7.0 29.0 2.0 34.0 74-75 18.631573256273157 17.5 9.0 30.0 2.0 34.5 76-77 19.7980823627679 22.0 8.0 30.0 2.0 34.5 78-79 22.922312126635283 27.5 10.5 33.5 2.0 36.0 80-81 26.521585417071506 31.5 23.5 35.0 2.0 36.0 82-83 27.7754632386588 33.5 27.5 35.0 2.0 36.0 84-85 27.8111479730958 34.0 28.0 35.0 2.0 36.0 86-87 27.566507500203727 34.0 27.0 35.0 2.0 36.0 88-89 27.14498774517486 33.5 26.5 35.0 2.0 35.5 90-91 25.87638121438735 33.0 21.5 35.0 2.0 35.0 92-93 23.552010919644705 31.5 2.0 34.5 2.0 35.0 94-95 16.232905537551165 8.5 2.0 32.5 2.0 35.0 96-97 13.635902602348162 2.0 2.0 30.0 2.0 34.0 98-99 13.332469919262328 2.0 2.0 30.5 2.0 34.0 100-101 13.057895037892797 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5268573916953407 1101 2 0.3969702383205096 1101 3 0.4885357083975812 1101 4 0.40132031286863423 1101 5 0.5231358151255776 1101 6 0.4278566179062011 1101 7 0.5423791410133845 1101 8 0.4210356948701204 1101 9 0.5239044677234475 1101 10-11 0.6139942142095194 1101 12-13 0.6478940939739957 1101 14-15 0.8713781017043303 1101 16-17 0.8774131643504361 1101 18-19 0.6176581098234735 1101 20-21 0.6004695379448677 1101 22-23 0.7526908493722644 1101 24-25 0.7109765256503806 1101 26-27 0.59613540040516 1101 28-29 0.6056679738349828 1101 30-31 0.6955464905340065 1101 32-33 0.8735637965543148 1101 34-35 0.7638728303802722 1101 36-37 0.6447157373797765 1101 38-39 0.7497950262140165 1101 40-41 0.8967990068070932 1101 42-43 0.8030917565239193 1101 44-45 0.799995858408522 1101 46-47 0.7837499044544245 1101 48-49 0.7438625798983978 1101 50-51 0.6764238561690021 1101 52-53 0.5391840618303547 1101 54-55 0.4490144189953966 1101 56-57 -1.6986080434287558 1101 58-59 -9.242161281502419 1101 60-61 -9.221387304927315 1101 62-63 -2.9921361161887674 1101 64-65 -4.398247698325751 1101 66-67 -9.167993051189063 1101 68-69 -4.388329910441076 1101 70-71 -4.369779652731353 1101 72-73 -4.4481198088853535 1101 74-75 -6.446661803452548 1101 76-77 -7.803869587567592 1101 78-79 -10.821068313142328 1101 80-81 -7.190541742446609 1101 82-83 -3.455681719491526 1101 84-85 -2.5969914096061153 1101 86-87 -2.4211263951673025 1101 88-89 -2.438946960609968 1101 90-91 -9.28422417237471 1101 92-93 -17.312791176509783 1101 94-95 -11.553648681793167 1101 96-97 -9.675548816586064 1101 98-99 -9.683118289001772 1101 100-101 -9.534162402901964 1102 1 0.3950061509611693 1102 2 0.4312806729364205 1102 3 0.4138790028915942 1102 4 0.3381896719772186 1102 5 0.29521333957121954 1102 6 0.30359926170648777 1102 7 0.43722698780233316 1102 8 0.3757909823387564 1102 9 0.48755867491941984 1102 10-11 0.4679889878598402 1102 12-13 0.46550640344778316 1102 14-15 0.5853241276529175 1102 16-17 0.5949489102073429 1102 18-19 0.5832017658312552 1102 20-21 0.6651805403655615 1102 22-23 0.647143460257567 1102 24-25 0.583613550465067 1102 26-27 0.5050082039063568 1102 28-29 0.5322101699728847 1102 30-31 0.554570480984701 1102 32-33 0.6370656526302341 1102 34-35 0.40214194935927594 1102 36-37 0.4621454661047508 1102 38-39 0.47428863142515354 1102 40-41 0.4269455914023226 1102 42-43 0.3958889556847822 1102 44-45 0.5174539928950566 1102 46-47 0.5424569300685107 1102 48-49 0.6693675000712247 1102 50-51 0.5307215081922152 1102 52-53 0.2791172850072883 1102 54-55 0.23685714110555978 1102 56-57 -2.2464831993305943 1102 58-59 -10.053271892451367 1102 60-61 -10.348376193307416 1102 62-63 -3.9760893832782074 1102 64-65 -4.75161731238769 1102 66-67 -6.311092895888709 1102 68-69 -3.9769314389194044 1102 70-71 -3.968635583520637 1102 72-73 -4.374313529970269 1102 74-75 -5.05086050097853 1102 76-77 -7.0212454540015266 1102 78-79 -9.706505587244735 1102 80-81 -5.04849915863991 1102 82-83 -2.6605501255324846 1102 84-85 -2.2891858788646395 1102 86-87 -2.189005920589299 1102 88-89 -2.3559982562929953 1102 90-91 -8.328711591959628 1102 92-93 -13.077011325818539 1102 94-95 -10.623171589177192 1102 96-97 -9.440214843217628 1102 98-99 -9.583514960715764 1102 100-101 -9.545062531814452 1103 1 0.15154950851277604 1103 2 0.1181171624310231 1103 3 0.17813976751899574 1103 4 0.12529122947291427 1103 5 0.24906142623324 1103 6 0.18591225428529157 1103 7 0.07108951341554359 1103 8 0.17853261358634143 1103 9 0.2947622252027102 1103 10-11 0.38413796386216603 1103 12-13 0.31144438673682373 1103 14-15 0.36214324086066796 1103 16-17 0.28647115787074284 1103 18-19 0.3538354871524163 1103 20-21 0.3452754906255038 1103 22-23 0.37444847248466573 1103 24-25 0.40961781844917056 1103 26-27 0.3651174161695607 1103 28-29 0.3748935499614916 1103 30-31 0.22441607621984616 1103 32-33 0.3342263461245736 1103 34-35 0.3232329041349189 1103 36-37 0.3464402755141336 1103 38-39 0.393963471906595 1103 40-41 0.30456433231162094 1103 42-43 0.33757859609119834 1103 44-45 0.3627402769531116 1103 46-47 0.3886153904799414 1103 48-49 0.37315259326970107 1103 50-51 0.2699766989597734 1103 52-53 0.26708234840772604 1103 54-55 0.06568408248978841 1103 56-57 -2.590659312067661 1103 58-59 -10.025957325035975 1103 60-61 -10.258576356807307 1103 62-63 -4.114961481774504 1103 64-65 -5.306664752128071 1103 66-67 -6.448877649347157 1103 68-69 -4.285324964040701 1103 70-71 -4.191674911967848 1103 72-73 -4.307241062714482 1103 74-75 -4.998235301469737 1103 76-77 -6.715788025904319 1103 78-79 -9.311442611475982 1103 80-81 -4.9965083174648335 1103 82-83 -2.709029611958808 1103 84-85 -2.17171474791963 1103 86-87 -2.020721006108232 1103 88-89 -1.8822001751163597 1103 90-91 -4.950160866296162 1103 92-93 -10.156316557461075 1103 94-95 -8.657734120806634 1103 96-97 -8.305432365309578 1103 98-99 -8.781673109698843 1103 100-101 -8.751256417201736 1104 1 0.1968328365547265 1104 2 0.24660665988603725 1104 3 0.19065679382912393 1104 4 0.1919165892017176 1104 5 0.23365018323578823 1104 6 0.19585561940107254 1104 7 0.20641104184051784 1104 8 0.2267818480424708 1104 9 0.16440572243241292 1104 10-11 0.21301868239805088 1104 12-13 0.24918493137392517 1104 14-15 0.3713118448568551 1104 16-17 0.336461940620147 1104 18-19 0.2329625111070257 1104 20-21 0.2253027327217083 1104 22-23 0.3334091762729585 1104 24-25 0.38503438696918835 1104 26-27 0.43325184464881517 1104 28-29 0.4558604395490633 1104 30-31 0.4478913893503318 1104 32-33 0.4028231733420924 1104 34-35 0.3989065315326883 1104 36-37 0.39215169363934166 1104 38-39 0.37065714170370967 1104 40-41 0.34423486680973525 1104 42-43 0.3226763501544667 1104 44-45 0.27169022728237735 1104 46-47 0.4038182212000123 1104 48-49 0.29509067382847576 1104 50-51 0.25278703866666774 1104 52-53 0.2550886571036983 1104 54-55 0.2980141010267019 1104 56-57 -2.50459323462227 1104 58-59 -10.349500743406978 1104 60-61 -10.602544178361335 1104 62-63 -4.041762174092437 1104 64-65 -5.20907557522855 1104 66-67 -6.34640332963146 1104 68-69 -3.8732252743548052 1104 70-71 -3.965043202577519 1104 72-73 -4.274196707256333 1104 74-75 -4.939754406781946 1104 76-77 -6.597183517644638 1104 78-79 -9.262013568943491 1104 80-81 -4.706433760148894 1104 82-83 -2.3025990434592707 1104 84-85 -1.8353455253435094 1104 86-87 -1.7344813371099441 1104 88-89 -1.748212587680495 1104 90-91 -4.1608329062785465 1104 92-93 -6.375360421332523 1104 94-95 -7.583920841820941 1104 96-97 -7.723157650276242 1104 98-99 -8.28588795356886 1104 100-101 -8.504321153025092 1105 1 0.3526465356136299 1105 2 0.29042055022198454 1105 3 0.27985913233587567 1105 4 0.19566706829999703 1105 5 0.2937427642224151 1105 6 0.1923430773509125 1105 7 0.2551597349618646 1105 8 0.2651150714881112 1105 9 0.39204032821695023 1105 10-11 0.5156313529081373 1105 12-13 0.3460753028208998 1105 14-15 0.5145403115177771 1105 16-17 0.4940931338483594 1105 18-19 0.5754135525163306 1105 20-21 0.5385427267225609 1105 22-23 0.5686532754848841 1105 24-25 0.5712255611276547 1105 26-27 0.6438364930721932 1105 28-29 0.6575430254407948 1105 30-31 0.6582068732993633 1105 32-33 0.5977232492008184 1105 34-35 0.5733875657618555 1105 36-37 0.45883978236870604 1105 38-39 0.45355581486413854 1105 40-41 0.46949010211310593 1105 42-43 0.5068323076011225 1105 44-45 0.40412610418979966 1105 46-47 0.5774631429032269 1105 48-49 0.3621358130368648 1105 50-51 0.4302795995112447 1105 52-53 0.4810107537319297 1105 54-55 0.3843438785874369 1105 56-57 1.5847354626578962 1105 58-59 -8.424792748823599 1105 60-61 -9.672981297120469 1105 62-63 -3.335983651171432 1105 64-65 -4.371421095469504 1105 66-67 -5.813292186801849 1105 68-69 -3.811127617281212 1105 70-71 -3.7266417280054096 1105 72-73 -2.148302432743346 1105 74-75 -4.353756610815138 1105 76-77 -6.038081447432923 1105 78-79 -8.809541639211723 1105 80-81 -4.225410109177332 1105 82-83 -1.8378682840097191 1105 84-85 -1.2778960453055177 1105 86-87 -1.138033511183643 1105 88-89 -1.3044865833492274 1105 90-91 -4.097014480358187 1105 92-93 -6.2317168689990545 1105 94-95 -7.6355791195885345 1105 96-97 -8.114722767429807 1105 98-99 -8.41906394221396 1105 100-101 -8.387401466922313 1106 1 0.2858419222572479 1106 2 0.2659830322147023 1106 3 0.24163829540108495 1106 4 0.16706059179021793 1106 5 0.08537778172767219 1106 6 0.27948958895851206 1106 7 0.24437806615615187 1106 8 0.22528602061372283 1106 9 0.22941253108589166 1106 10-11 0.339305362180248 1106 12-13 0.35141811780644616 1106 14-15 0.4090412151559022 1106 16-17 0.29861568987829656 1106 18-19 0.2488919917979544 1106 20-21 0.2771014457391274 1106 22-23 0.3013758891381144 1106 24-25 0.45657309074690744 1106 26-27 0.4292241843859159 1106 28-29 0.49929809148931525 1106 30-31 0.4936823091847131 1106 32-33 0.5432626818732373 1106 34-35 0.5262141632805637 1106 36-37 0.5065793774340577 1106 38-39 0.593349942525947 1106 40-41 0.44863090377544523 1106 42-43 0.5544391944586451 1106 44-45 0.5391863903855239 1106 46-47 0.6521359560553037 1106 48-49 0.74641381927065 1106 50-51 0.7322719687892985 1106 52-53 0.7306602119855938 1106 54-55 0.7130695305601691 1106 56-57 1.8967756767371924 1106 58-59 -8.484051329020389 1106 60-61 -9.745013478860866 1106 62-63 -3.385478574209401 1106 64-65 -4.949402009933824 1106 66-67 -6.625998997701384 1106 68-69 -3.941451442769921 1106 70-71 -4.052400216528769 1106 72-73 -4.074589134857076 1106 74-75 -4.887499418816045 1106 76-77 -6.492611424645526 1106 78-79 -8.970135159209626 1106 80-81 -4.4673982876698695 1106 82-83 -1.836833247296692 1106 84-85 -1.422799002428544 1106 86-87 -1.3150793566309673 1106 88-89 -1.4201164671422397 1106 90-91 -3.8714598560883005 1106 92-93 -6.024239372644164 1106 94-95 -7.42324336782273 1106 96-97 -7.316980116583357 1106 98-99 -6.774169715894081 1106 100-101 -7.230795741261608 1107 1 0.04302045854726444 1107 2 -0.024105012387835245 1107 3 0.05069250968499972 1107 4 -0.023082953707898923 1107 5 -0.013845205734170918 1107 6 -0.026211866678607976 1107 7 0.06840743117545145 1107 8 0.021282387827831428 1107 9 -0.041182085327235995 1107 10-11 0.05624701055684511 1107 12-13 0.09240174504224541 1107 14-15 0.11853060691654349 1107 16-17 0.06866538840751701 1107 18-19 0.07635232127145741 1107 20-21 0.09554035691246554 1107 22-23 0.05151211860908944 1107 24-25 0.15179875234280615 1107 26-27 0.1267203371647483 1107 28-29 0.20128098712240927 1107 30-31 0.16725757596742596 1107 32-33 0.23283482042365478 1107 34-35 0.14340682707128138 1107 36-37 0.20044526708818466 1107 38-39 -0.03230913060910012 1107 40-41 0.09145362886942365 1107 42-43 0.00258495999258912 1107 44-45 0.09685434366386403 1107 46-47 0.07210871016111042 1107 48-49 0.13077896470822736 1107 50-51 0.1266840377113212 1107 52-53 0.12108378472960624 1107 54-55 0.11337352410188117 1107 56-57 1.2790595385904169 1107 58-59 -9.04631576556016 1107 60-61 -10.451336796805315 1107 62-63 -3.9125176014300678 1107 64-65 -5.291080659748399 1107 66-67 -7.179529611490832 1107 68-69 -4.171648975502833 1107 70-71 -3.834839228612827 1107 72-73 -2.31935060714447 1107 74-75 -4.611104958814533 1107 76-77 -6.553536271387371 1107 78-79 -7.622913988140731 1107 80-81 -2.4342213659513625 1107 82-83 -1.1364939350032408 1107 84-85 -0.8885915299897853 1107 86-87 -0.8958079320238888 1107 88-89 -0.6803198022415096 1107 90-91 -0.062540609891375 1107 92-93 -2.4919920909266615 1107 94-95 -5.816232595603452 1107 96-97 -6.327889826717316 1107 98-99 -6.007045166838857 1107 100-101 -6.852212780036552 1108 1 -0.0910232709930483 1108 2 -0.07956762510257676 1108 3 -0.04595995701269118 1108 4 -0.016405388465607018 1108 5 -0.038739302604206216 1108 6 -0.11400824628315576 1108 7 -0.1791393949127169 1108 8 -0.07944192991735832 1108 9 -0.1520504313291653 1108 10-11 -0.1585865872857184 1108 12-13 -0.190207867183247 1108 14-15 -0.23160840958644968 1108 16-17 -0.28490946372586023 1108 18-19 -0.17590953092953754 1108 20-21 -0.19075707078503257 1108 22-23 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11.96436777842246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 18.0 4 43.0 5 95.0 6 246.0 7 583.0 8 1296.0 9 2245.0 10 3846.0 11 6295.0 12 9498.0 13 13567.0 14 16363.0 15 21940.0 16 29718.0 17 39738.0 18 42651.0 19 40010.0 20 40736.0 21 41087.0 22 41373.0 23 35293.0 24 35913.0 25 42420.0 26 60197.0 27 89842.0 28 126875.0 29 173041.0 30 216327.0 31 243585.0 32 248523.0 33 231392.0 34 208017.0 35 188087.0 36 155708.0 37 102072.0 38 40691.0 39 3127.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58365248100495 19.78106855068619 14.174388845107998 22.460890123200862 2 15.116362898340851 22.022909765957728 39.84779847304796 23.01292886265346 3 19.149408805622812 27.024752591617496 30.68655336420551 23.13928523855418 4 12.224619034783645 17.367061788138834 38.63208256805973 31.776236609017793 5 12.464981324026667 39.409697226006934 34.450099392035376 13.675222057931022 6 29.739146174049857 37.82204176043227 18.17220536704925 14.266606698468618 7 26.808380767909266 34.36731502082498 21.701744550263804 17.122559661001944 8 25.461737910110475 34.93336916857792 20.63020926790319 18.97468365340841 9 26.871720815651074 16.53539481849695 20.914810159908228 35.67807420594375 10-11 24.16512044831974 27.065494361526742 28.177811714484513 20.591573475669 12-13 26.133551736674836 24.746578208350833 28.289350995743725 20.83051905923061 14-15 22.874762384600796 25.59899453229924 27.648970947060935 23.877272136039025 16-17 21.083294416689128 29.061467664186445 28.11357966263187 21.74165825649255 18-19 20.8665912753596 28.005741910599596 30.64454866196754 20.483118152073267 20-21 22.17484751988667 26.840966219308086 30.058817675783082 20.92536858502216 22-23 21.60264466850751 26.956096402837108 29.75385275985169 21.687406168803694 24-25 21.62869322127427 26.957623488053795 30.26699500306273 21.146688287609205 26-27 21.709768274254962 26.816671152540472 30.409444703671795 21.064115869532767 28-29 21.100289846895958 27.210621294147973 30.626254220567294 21.062834638388768 30-31 21.276429009575676 27.369405364549447 30.27425769750016 21.079907928374716 32-33 21.141844984446966 27.40701823052109 29.97384221998511 21.477294565046837 34-35 21.735453537298454 26.917726680495672 30.013326858291595 21.333492923914278 36-37 21.864045907086155 26.816697949601664 30.16164505621124 21.157611087100946 38-39 21.83600840592368 26.88662180030068 30.375723297103363 20.90164649667228 40-41 21.530782662993342 26.939987888068178 30.458880619026402 21.070348829912078 42-43 21.132913668853114 27.45849475406325 29.81658735036754 21.592004226716092 44-45 21.37649057286084 27.529850678947774 29.429790552034095 21.663868196157292 46-47 21.55769338998928 27.440208975178805 28.86160806643249 22.140489568399428 48-49 21.850063544361532 27.450536680715516 28.851865847175468 21.84753392774748 50-51 21.767077109093837 27.780722442205974 28.886667807928397 21.565532640771792 52-53 21.764644644519045 27.914955202794623 28.785177113597022 21.53522303908931 54-55 21.185517012632936 27.600177054018726 29.07192878319665 22.142377150151688 56-57 20.80114765387276 28.064553363620178 29.331889114228037 21.802409868279025 58-59 20.39998455565874 27.325657071139897 30.42533238172839 21.849025991472974 60-61 21.507017716936623 27.534178842346968 29.210211719883638 21.748591720832763 62-63 22.068047765589178 27.349672721830302 28.899486138109758 21.682793374470755 64-65 21.33584347332875 27.53955549419019 29.310624136804332 21.813976895676735 66-67 21.056123420037824 27.62773156285568 29.287913849784335 22.02823116732216 68-69 21.271865598125615 27.59103455008564 29.3772545336751 21.759845318113644 70-71 21.122475814878143 27.61393280662281 29.14471171818401 22.118879660315038 72-73 20.351952710818527 27.505226074237417 29.727479593663926 22.41534162128013 74-75 20.91877778844001 27.7518352365416 29.145515465566323 22.183871509452068 76-77 19.36995336115411 27.906354331642667 30.084727998348477 22.638964308854746 78-79 20.00533920250112 27.805755858044446 29.60660953719991 22.582295402254527 80-81 20.92588563868881 28.308432554265273 28.41297466803861 22.3527071390073 82-83 21.31650431652802 28.178697658510536 27.92607827009001 22.578719754871436 84-85 21.24872818820738 27.768945343337275 27.921621525265223 23.06070494319012 86-87 21.25703219623919 27.8350874931659 28.191430490588182 22.716449820006726 88-89 21.67520744693355 27.410321807197764 28.106434576839597 22.808036169029094 90-91 20.67161013620725 27.73855906241654 28.74202708434795 22.847803717028263 92-93 19.7260550367785 28.177912604748524 29.279419064093155 22.816613294379824 94-95 18.904454132130297 28.333542563892617 29.202195881383062 23.559807422594023 96-97 18.921511700281336 28.302899029105937 28.67355986189516 24.102029408717566 98-99 19.2633436767759 28.07127914259644 28.541244742171678 24.124132438455987 100-101 19.835748249624057 28.072066998007365 28.47037591058705 23.62180884178152 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1312.0 1 953.0 2 638.5 3 723.0 4 827.0 5 955.0 6 1286.5 7 1837.5 8 2351.5 9 2757.5 10 2961.5 11 3188.0 12 3514.5 13 3844.5 14 4251.5 15 4574.5 16 4865.5 17 5340.5 18 5765.5 19 6185.0 20 6768.0 21 7519.0 22 8607.5 23 10071.0 24 11819.5 25 14016.5 26 17142.0 27 21089.0 28 25216.0 29 29668.5 30 34673.0 31 39799.0 32 45463.5 33 51951.0 34 58453.5 35 64466.0 36 70680.0 37 76353.0 38 79902.5 39 82407.0 40 84460.0 41 86282.5 42 88745.0 43 91574.0 44 94316.0 45 96968.5 46 101088.0 47 106340.0 48 111252.5 49 107965.5 50 97711.0 51 89455.5 52 82131.5 53 75713.5 54 69534.0 55 62730.5 56 55474.5 57 48223.0 58 41590.5 59 35119.5 60 28862.0 61 22942.0 62 17637.0 63 13082.0 64 9268.0 65 6548.5 66 4556.5 67 3045.0 68 2009.0 69 1312.5 70 917.0 71 668.5 72 486.5 73 346.0 74 233.5 75 155.0 76 86.0 77 46.0 78 22.0 79 11.0 80 6.5 81 3.0 82 0.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02409436528781601 2 0.007443787649894377 3 1.5671131894514476E-4 4 0.0 5 0.13582953569570422 6 0.0 7 3.917782973628619E-5 8 0.00164546884892402 9 0.0 10-11 0.0 12-13 0.0 14-15 7.835565947257239E-4 16-17 0.0 18-19 1.3712240407700167E-4 20-21 0.0 22-23 3.134226378902895E-4 24-25 1.9588914868143095E-5 26-27 2.7424480815400334E-4 28-29 0.003212582038375468 30-31 0.012556494430479724 32-33 0.0026836813369356042 34-35 0.0038981940587604763 36-37 0.10256755824959725 38-39 0.1016860570805308 40-41 0.11408584019206539 42-43 0.11624062082756113 44-45 0.11193105955656965 46-47 0.10529041741626913 48-49 0.10462439431075227 50-51 0.1039975490349717 52-53 0.08374261106131174 54-55 0.07169542841740373 56-57 0.5511537087300742 58-59 1.5756147785042216 60-61 0.5204187013019577 62-63 0.0048776398021676305 64-65 0.12031511512013489 66-67 1.915149439913746 68-69 2.882312933698575 70-71 2.7950051401312614 72-73 2.637804098314413 74-75 2.0146807163587814 76-77 1.4103822815914349 78-79 0.9401307912667917 80-81 0.05245911401688721 82-83 2.9383372302214646E-4 84-85 1.1753348920885858E-4 86-87 2.9383372302214646E-4 88-89 1.5671131894514476E-4 90-91 0.24854415184699963 92-93 0.38966269455710245 94-95 2.0073152843683593 96-97 2.436155808661748 98-99 1.4188642817293409 100-101 0.9461445881313115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2552464.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.714578036333386 #Duplication Level Percentage of deduplicated Percentage of total 1 69.17539631271923 30.931486566193723 2 15.141361658258953 13.540791948891323 3 6.126952025226681 8.21892223370608 4 3.1112874115638065 5.564796150313261 5 1.77394024823402 3.9660494830726347 6 1.1108564974362494 2.9802887725068685 7 0.7290850153581173 2.28205101700463 8 0.5080364721531394 1.817330918351605 9 0.36977825676320203 1.4881030846359766 >10 1.7525103994716364 13.82120252282982 >50 0.12373583330054848 3.80671050796325 >100 0.06923884531669212 5.667074036592197 >500 0.0044817104954115485 1.3060042095396387 >1k 0.0028120536441780886 2.437308695408294 >5k 3.515067055222611E-4 1.1916595860844674 >10k+ 1.7575335276113054E-4 0.9802202669061603 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14433 0.5654536165838187 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10513 0.41187652401757674 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8941 0.35028897567213485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7934 0.31083690112769463 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7858 0.3078593860677369 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5594 0.21916077954478494 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4985 0.19530148123538668 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3688 0.14448783606742346 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3614 0.1415886766669383 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3277 0.12838574804580985 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2859 0.11200941521604221 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2693 0.10550589547981871 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.917782973628619E-5 0.0 5 0.0 0.0 0.0 3.917782973628619E-5 0.0 6 0.0 0.0 0.0 3.917782973628619E-5 0.0 7 0.0 0.0 0.0 3.917782973628619E-5 0.0 8 0.0 0.0 0.0 3.917782973628619E-5 0.0 9 0.0 0.0 0.0 7.835565947257238E-5 3.917782973628619E-5 10-11 0.0 0.0 0.0 1.1753348920885858E-4 3.917782973628619E-5 12-13 0.0 0.0 0.0 1.1753348920885858E-4 3.917782973628619E-5 14-15 0.0 0.0 0.0 2.1547806354957406E-4 1.5671131894514476E-4 16-17 0.0 0.0 0.0 3.3301155275843264E-4 1.5671131894514476E-4 18-19 0.0 0.0 0.0 4.897228717035774E-4 1.9588914868143097E-4 20-21 0.0 0.0 0.0 6.856120203850084E-4 1.9588914868143097E-4 22-23 0.0 0.0 0.0 0.0014104018705063028 2.9383372302214646E-4 24-25 0.0 0.0 0.0 0.0026053256774630318 3.9177829736286194E-4 26-27 0.0 0.0 0.0 0.00334970444245247 3.9177829736286194E-4 28-29 0.0 0.0 0.0 0.005582840737420783 3.9177829736286194E-4 30-31 0.0 0.0 0.0 0.013594706918491309 4.1136721223100506E-4 32-33 0.0 0.0 0.0 0.03241965410677682 4.309561270991481E-4 34-35 0.0 0.0 0.0 0.06025550213440816 4.309561270991481E-4 36-37 0.0 0.0 0.0 0.10274385848341054 4.505450419672912E-4 38-39 0.0 0.0 0.0 0.164272640084248 5.289007014398636E-4 40-41 0.0 0.0 0.0 0.23634025788414645 5.484896163080067E-4 42-43 0.0 0.0 0.0 0.3078202082380006 5.484896163080067E-4 44-45 0.0 0.0 0.0 0.3916999417033894 5.680785311761498E-4 46-47 0.0 0.0 0.0 0.4893506823210827 6.26845275780579E-4 48-49 0.0 0.0 0.0 0.5814381711162234 6.26845275780579E-4 50-51 0.0 0.0 0.0 0.6756804405468598 6.26845275780579E-4 52-53 1.9588914868143095E-5 0.0 0.0 0.7600890747136884 6.660231055168652E-4 54-55 7.835565947257238E-5 0.0 0.0 0.8563490023757436 7.247898501212945E-4 56-57 7.835565947257238E-5 0.0 0.0 0.9372512207811746 7.639676798575808E-4 58-59 7.835565947257238E-5 0.0 0.0 0.9962726212788897 7.835565947257239E-4 60-61 7.835565947257238E-5 0.0 0.0 1.0473996890847432 7.835565947257239E-4 62-63 1.9588914868143097E-4 0.0 0.0 1.092708849174758 8.2273442446201E-4 64-65 2.546558932858602E-4 0.0 0.0 1.135001316375079 8.2273442446201E-4 66-67 2.7424480815400334E-4 0.0 0.0 1.1707902638391765 8.423233393301531E-4 68-69 2.7424480815400334E-4 0.0 0.0 1.2205852854339962 8.619122541982963E-4 70-71 2.7424480815400334E-4 0.0 0.0 1.30103695879746 9.010900839345824E-4 72-73 2.7424480815400334E-4 0.0 0.0 1.4155145772868882 9.402679136708686E-4 74-75 2.7424480815400334E-4 0.0 0.0 1.5562609306144966 9.402679136708686E-4 76-77 2.7424480815400334E-4 0.0 0.0 1.7728751512264227 9.794457434071549E-4 78-79 2.7424480815400334E-4 0.0 0.0 2.1094714754057255 0.001018623573143441 80-81 2.7424480815400334E-4 0.0 0.0 2.5152362579844416 0.0010578014028797271 82-83 2.7424480815400334E-4 0.0 0.0 2.9185524262046396 0.001194923806956729 84-85 2.7424480815400334E-4 3.917782973628619E-5 0.0 3.2417107547843966 0.0012341016366930152 86-87 2.7424480815400334E-4 7.835565947257238E-5 0.0 3.491293119119408 0.0012732794664293012 88-89 2.7424480815400334E-4 7.835565947257238E-5 0.0 3.7180739865479007 0.0012928683812974443 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14280 0.0 26.897448 1 TACATGG 14335 0.0 26.72071 2 ACATGGG 13950 0.0 25.735287 3 CATGGGA 9915 0.0 23.20571 4 GAGTACT 10840 0.0 21.48069 12-13 AGTACTT 11260 0.0 20.971598 12-13 CATGGGG 4145 0.0 20.659773 4 ATGGGAT 4145 0.0 19.638136 5 GTATCAA 27745 0.0 19.578081 1 GTACTTT 12105 0.0 19.294142 14-15 AGTACAT 10390 0.0 18.704918 2 ATGGGAG 4160 0.0 18.32316 5 ATCAACG 30900 0.0 17.435148 3 TCAACGC 31015 0.0 17.431185 4 ACTTTTT 13745 0.0 17.2317 16-17 AACGCAG 31310 0.0 17.230293 6 CAACGCA 31355 0.0 17.212156 5 TACTTTT 13475 0.0 17.175575 14-15 TATCAAC 31935 0.0 16.901209 2 ATGGGAC 1915 0.0 16.216377 5 >>END_MODULE