FastQCFastQC Report
Fri 27 May 2016
SRR939682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582843
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57410.22227444718862124No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38950.15080281689595534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.1505705147389911No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30750.11905485544417528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28760.1113501672381945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG114650.035.7271231
CGTATAG1603.2047217E-431.4505121
GTATAGG7050.028.5508211
TACATGG140100.028.4925272
ACATGGG136500.027.9288643
CATGGGA96300.025.554354
GAGTACT99050.023.20775412-13
CATGGGG42450.022.253884
ATGGGAT40400.022.0790375
GTATCAA261450.021.8643911
ATGGGAG42350.021.0624085
AGTACTT107500.020.98833812-13
GTACTTT112250.020.6480514-15
GTCGGGA6150.019.9714972
TATCAAC310200.017.9092732
AGTACAT96750.017.675432
ACTTTTT139700.017.65798416-17
ATCAACG325650.017.0740973
CAACGCA327200.016.9770765
TCAACGC327750.016.9768074
Produced by FastQC (version 0.11.5)