FastQCFastQC Report
Fri 27 May 2016
SRR939682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582843
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144100.5579123469757937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106290.411523271062159No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA89830.34779504600163463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79820.3090393028147665No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA77640.3005989911117323No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57620.22308750473799607No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT47520.1839833083156816No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG37250.14422092244863507No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA37020.1433304308469388No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT31130.12052610243828216No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC29080.11258911207533714No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27270.1055813303402491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG143850.028.2102151
TACATGG142200.027.5383192
ACATGGG140650.026.2946833
CATGGGA100900.024.8720764
ATGGGAT41350.021.1051225
AGTACTT113850.020.70705412-13
ATGGGAG41050.020.5698665
CATGGGG41200.020.4949764
GAGTACT110600.020.35633712-13
GTACTTT120200.018.82860614-15
GTATCAA294400.017.4224621
TACTTTT135150.017.32146314-15
AGTACAT101300.016.999742
ACTTTTT140700.016.36932816-17
ATGGGAC21000.015.9488965
ATCAACG327700.015.61873
TCAACGC327950.015.592414
AACGCAG329550.015.4796836
CAACGCA330550.015.4412225
TATCAAC337800.015.1680262
Produced by FastQC (version 0.11.5)