FastQCFastQC Report
Fri 27 May 2016
SRR939681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789867
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA109290.6106040281205252No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91870.5132783609061455No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA91080.5088646251369515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82260.45958722072645625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56470.31549830238783105No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45250.25281208045067033No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT43340.2421408965023658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40730.2275588074421172No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37120.20738971107909138No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA36830.20576947896128595No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG36760.20537838845009151No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT33560.18749996508120437No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32820.1833655796771492No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG29210.16319648331412334No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC27870.1557098935284018No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26210.1464354614057916No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22740.12704854606515456No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG20590.11503648036418349No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20190.11280167744307258No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18980.10604139860671212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97000.027.8394661
TACATGG98750.027.0104472
ACATGGG101350.025.4746193
CCGATCG2002.0794687E-823.724299
CATGGGA71200.022.9963824
GAGTACT69100.021.90462912-13
CATGGGG32450.021.206734
AGTACTT70600.021.10319512-13
CCGTGCG1353.824955E-421.0882599
GTACTTT76400.019.90675214-15
ATGGGAG27250.019.5056215
TAGGACG2201.3567278E-619.4151274
TAGCCCT11600.019.2291584
AGTACAT71150.017.8102052
ATGGGAT31000.017.7584295
GGGATCG3501.3315002E-917.6237587
ACTTTTT90950.017.61130116-17
TACTTTT87100.017.3250614-15
GTATAGG5100.016.7503051
GTATAGC6600.016.5388131
Produced by FastQC (version 0.11.5)