FastQCFastQC Report
Fri 27 May 2016
SRR939681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789867
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA107460.6003798047564428No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA97070.5423307988805872No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96330.5381964134765321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84080.4697555740175108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67000.37432948928607546No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT59600.3329856352455238No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51700.2888482775535836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47760.26683546878064124No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG44380.24795138409725417No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA43340.2421408965023658No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41080.22951425999808922No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT38940.2175580643701459No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36580.20437272713559165No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC35330.19738896800712008No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG27330.15269290958490211No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26660.14894961469204138No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24910.1391723519121812No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG23200.1296185694244321No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23030.12866877818295996No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22040.12313764095321048No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19530.1091142526232396No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT19350.1081085913087397No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG19210.1073264102863509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96350.027.4961431
TACATGG97400.027.241942
ACATGGG94900.025.9256153
CATGGGG31150.023.5591774
CATGGGA66250.022.2965344
GAGTACT66050.020.7669312-13
CGCGAGA1001.0478139E-519.45643268-69
ATGGGAG25050.019.155175
AGTACTT68500.018.75551412-13
GTACTTT72600.018.7315714-15
TACGCGG1353.7898826E-718.02199264-65
ATGGGAT29800.017.3648095
TTACGCG1153.507389E-516.90763362-63
GCTTACG4300.016.76343260-61
AGTACAT71400.016.7360652
ATGGGGA17600.016.571915
TACACTG14000.016.4649945
ACTTTTT84350.016.37285816-17
TACTTTT81050.015.88034114-15
ATGGGGG16400.015.4897175
Produced by FastQC (version 0.11.5)