##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939681_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1789867 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55839344487607 31.0 31.0 34.0 30.0 34.0 2 31.750512188894483 31.0 31.0 34.0 30.0 34.0 3 31.80009576130517 31.0 31.0 34.0 30.0 34.0 4 35.56356477883552 37.0 35.0 37.0 33.0 37.0 5 35.319154439966766 37.0 35.0 37.0 33.0 37.0 6 35.35781038479396 37.0 35.0 37.0 33.0 37.0 7 35.292234562679795 37.0 35.0 37.0 32.0 37.0 8 35.263404487596006 37.0 35.0 37.0 32.0 37.0 9 36.864194378688474 39.0 37.0 39.0 33.0 39.0 10-11 36.741571301107854 39.0 35.0 39.0 32.0 39.0 12-13 36.68977303900234 39.0 35.0 39.0 32.0 39.0 14-15 37.68742035022714 39.0 36.5 41.0 32.0 41.0 16-17 37.66709397960854 39.0 36.5 41.0 32.0 41.0 18-19 37.700506518082065 39.5 37.0 41.0 32.0 41.0 20-21 37.64776740394677 39.0 36.5 41.0 32.0 41.0 22-23 37.51326690754118 39.0 36.0 41.0 32.0 41.0 24-25 37.37725708111273 39.0 36.0 41.0 31.5 41.0 26-27 37.072295315797206 39.0 36.0 40.5 31.0 41.0 28-29 36.882516410437205 39.0 36.0 40.0 30.5 41.0 30-31 36.65992473183762 38.5 35.5 40.0 30.0 41.0 32-33 36.49008501749013 38.0 35.0 40.0 30.0 41.0 34-35 36.4704519944778 38.0 35.0 40.0 30.0 41.0 36-37 36.33627023683883 38.0 35.0 40.0 30.0 41.0 38-39 36.33931515581884 38.0 35.0 40.0 30.0 41.0 40-41 36.24218531321042 38.0 35.0 40.0 30.0 41.0 42-43 36.07213776219127 38.0 35.0 40.0 29.5 41.0 44-45 35.852976226725225 38.0 35.0 40.0 29.0 41.0 46-47 35.520349277348544 38.0 34.5 40.0 27.5 41.0 48-49 35.206229010311944 38.0 34.0 40.0 27.0 41.0 50-51 34.94872496112839 38.0 34.0 40.0 26.0 41.0 52-53 34.677288591833914 38.0 34.0 40.0 26.0 41.0 54-55 34.40172677634707 38.0 33.0 40.0 24.0 41.0 56-57 32.33569700988956 37.0 30.5 39.5 15.5 41.0 58-59 27.221547466934695 32.5 17.5 38.5 6.5 40.0 60-61 27.989462624876598 32.5 19.0 38.0 7.5 40.0 62-63 31.153366143964888 34.5 29.0 38.5 8.0 40.0 64-65 31.376509260185255 35.5 30.5 39.0 4.5 40.0 66-67 24.632166803455227 27.0 17.5 35.5 2.0 38.5 68-69 17.182550994012402 13.0 7.0 28.0 2.0 35.0 70-71 16.05878844629238 12.5 7.0 26.5 2.0 33.5 72-73 17.138018355553793 13.0 7.0 28.0 2.0 34.0 74-75 18.150060311743836 17.0 8.5 29.0 2.0 34.5 76-77 19.31094237728278 21.0 8.0 30.0 2.0 34.0 78-79 22.36889640403449 27.5 10.0 33.5 2.0 35.5 80-81 26.042074075895023 31.5 22.5 34.5 2.0 36.0 82-83 27.403696475771664 33.0 26.0 35.0 2.0 36.0 84-85 27.47523307597715 34.0 27.0 35.0 2.0 36.0 86-87 27.242988445510196 34.0 26.0 35.0 2.0 36.0 88-89 26.788906661779897 33.0 25.5 35.0 2.0 35.0 90-91 25.38136828043648 32.5 16.0 35.0 2.0 35.0 92-93 22.866016581120274 31.5 2.0 34.5 2.0 35.0 94-95 15.575233802288103 4.5 2.0 32.0 2.0 34.5 96-97 13.018933529698018 2.0 2.0 30.0 2.0 34.0 98-99 12.6719038341955 2.0 2.0 29.5 2.0 34.0 100-101 12.394980185678602 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3729083960594508 1101 2 0.3783862402452627 1101 3 0.36556584257114366 1101 4 0.2712745007710211 1101 5 0.40499413178392984 1101 6 0.39546877358330335 1101 7 0.38027995042235574 1101 8 0.3067869616019365 1101 9 0.48113053568063435 1101 10-11 0.5247369145562928 1101 12-13 0.4842242551089484 1101 14-15 0.681029367987442 1101 16-17 0.7203553343739415 1101 18-19 0.5773381800611261 1101 20-21 0.5369076285291996 1101 22-23 0.6159737646841776 1101 24-25 0.5965657259678139 1101 26-27 0.709241853988992 1101 28-29 0.7606911280931143 1101 30-31 0.6638430499902768 1101 32-33 0.48873936356232406 1101 34-35 0.45574273940928123 1101 36-37 0.28373554881037677 1101 38-39 0.6140284818758559 1101 40-41 0.47652672945771 1101 42-43 0.6037118007014257 1101 44-45 0.43030533718224007 1101 46-47 0.6017283736209151 1101 48-49 0.5809691359252156 1101 50-51 0.3830806411926986 1101 52-53 0.2073635898162749 1101 54-55 0.19416374640669432 1101 56-57 -1.7528304613182897 1101 58-59 -9.890839169513963 1101 60-61 -10.185267148498621 1101 62-63 -3.9253458311999836 1101 64-65 -5.1653079081556115 1101 66-67 -9.517108519246795 1101 68-69 -4.953976128127211 1101 70-71 -4.696301966510447 1101 72-73 -4.573122462197581 1101 74-75 -6.558330334929465 1101 76-77 -7.970309135987648 1101 78-79 -11.111450835583558 1101 80-81 -7.8551021731964035 1101 82-83 -4.126151604582024 1101 84-85 -3.4855646710381976 1101 86-87 -3.204551140654015 1101 88-89 -3.302428859276251 1101 90-91 -9.529430919703607 1101 92-93 -16.785621737008384 1101 94-95 -10.957826939132284 1101 96-97 -9.185135316301174 1101 98-99 -9.222725889730436 1101 100-101 -9.11608297151468 1102 1 0.34680409669465817 1102 2 0.30595115549666474 1102 3 0.3869877482453674 1102 4 0.306249708632933 1102 5 0.26221621944416285 1102 6 0.21216006145795774 1102 7 0.3532075320669321 1102 8 0.4366501957012545 1102 9 0.4792328478920993 1102 10-11 0.4252808723227375 1102 12-13 0.30740488603741056 1102 14-15 0.42754493971953167 1102 16-17 0.5173660215056657 1102 18-19 0.5223005063397821 1102 20-21 0.4599347133127125 1102 22-23 0.5470711141038507 1102 24-25 0.4256414450925661 1102 26-27 0.5305371622850785 1102 28-29 0.49345410041480875 1102 30-31 0.4050097824832122 1102 32-33 0.33474752747483194 1102 34-35 0.09809059889163763 1102 36-37 0.20777566719583973 1102 38-39 0.08087258853332457 1102 40-41 0.19819051940717003 1102 42-43 0.2150840348160159 1102 44-45 0.30883568018439433 1102 46-47 0.16879443483134082 1102 48-49 0.2488444966059049 1102 50-51 0.3149600867390987 1102 52-53 0.20492038043272487 1102 54-55 0.0038268977322033493 1102 56-57 -2.957541804021499 1102 58-59 -10.334645717373302 1102 60-61 -10.550543135147162 1102 62-63 -4.113518413866508 1102 64-65 -4.965079661920257 1102 66-67 -6.682602132643641 1102 68-69 -4.27437623548586 1102 70-71 -3.9507514224799447 1102 72-73 -4.17603383821398 1102 74-75 -5.234593999336219 1102 76-77 -7.136500835185586 1102 78-79 -9.699482019052653 1102 80-81 -5.4302635930363685 1102 82-83 -3.0200847498195103 1102 84-85 -2.476781773358411 1102 86-87 -2.356815673531141 1102 88-89 -2.2922170586985793 1102 90-91 -8.711870979609152 1102 92-93 -13.375033635634468 1102 94-95 -10.68749662216969 1102 96-97 -9.252310663671143 1102 98-99 -9.278502563372506 1102 100-101 -9.200276046881763 1103 1 0.16268224616567295 1103 2 0.20259514790792466 1103 3 0.21652429324057465 1103 4 0.24744909688782002 1103 5 0.17860339021977722 1103 6 0.1477568275332075 1103 7 0.09169798806871654 1103 8 0.2139216140400606 1103 9 0.31924685898481897 1103 10-11 0.3880604287107374 1103 12-13 0.32227110309077034 1103 14-15 0.44623365631311884 1103 16-17 0.3441248492200444 1103 18-19 0.2852870832773746 1103 20-21 0.2715829163114236 1103 22-23 0.28986895266896084 1103 24-25 0.42788890664071033 1103 26-27 0.34743135796608016 1103 28-29 0.21441284391742954 1103 30-31 0.48574537695040476 1103 32-33 0.4448525743720637 1103 34-35 0.333639088403892 1103 36-37 0.10950533538218821 1103 38-39 0.20884994573633264 1103 40-41 0.25783385361165756 1103 42-43 0.15967185702275088 1103 44-45 -0.05899391927741959 1103 46-47 0.18596951783315774 1103 48-49 0.03731238724678576 1103 50-51 0.14419051718696352 1103 52-53 0.2160124955027598 1103 54-55 0.09809172674362543 1103 56-57 -2.565358251447229 1103 58-59 -10.286956579720972 1103 60-61 -10.468073395719607 1103 62-63 -4.544937563327842 1103 64-65 -5.565845492761113 1103 66-67 -6.832753435505872 1103 68-69 -4.138528830562862 1103 70-71 -4.052600753625708 1103 72-73 -4.453109246447372 1103 74-75 -5.019263589215321 1103 76-77 -6.475529048541375 1103 78-79 -8.975334784005078 1103 80-81 -4.993932394035294 1103 82-83 -2.972484542677968 1103 84-85 -2.6231798456837225 1103 86-87 -2.376726428436239 1103 88-89 -2.4260084749078885 1103 90-91 -5.028927609590472 1103 92-93 -9.641560796343395 1103 94-95 -8.109669115902207 1103 96-97 -7.69882349870323 1103 98-99 -7.837235116053245 1103 100-101 -7.9935501174066825 1104 1 0.23856259696773563 1104 2 0.33039099217036494 1104 3 0.15919331524154146 1104 4 0.11716074687086575 1104 5 0.18180851842490853 1104 6 0.17525914718062552 1104 7 0.24517054485546907 1104 8 0.2202393075967919 1104 9 0.40426305190811007 1104 10-11 0.29505427718422794 1104 12-13 0.31619596881886736 1104 14-15 0.4216944631935533 1104 16-17 0.3607744530956367 1104 18-19 0.31628281423396487 1104 20-21 0.26885175452493826 1104 22-23 0.35021658752076945 1104 24-25 0.5246393928744979 1104 26-27 0.41036684946367785 1104 28-29 0.420061296891042 1104 30-31 0.39293940653482906 1104 32-33 0.21858323654218026 1104 34-35 0.22825572206019018 1104 36-37 0.18338666311268526 1104 38-39 0.0683713177185652 1104 40-41 -0.05170190464539104 1104 42-43 -0.05996195094612489 1104 44-45 0.1361904703876533 1104 46-47 -0.001047594027383525 1104 48-49 0.031512711105250446 1104 50-51 0.027184379043042384 1104 52-53 0.06877622157023922 1104 54-55 0.014578286171207822 1104 56-57 -3.324170681188683 1104 58-59 -10.295634068434996 1104 60-61 -10.735527069684242 1104 62-63 -4.668544102057034 1104 64-65 -5.974282429577485 1104 66-67 -7.003398632051383 1104 68-69 -4.340251726375005 1104 70-71 -4.319647374077862 1104 72-73 -4.52695544490213 1104 74-75 -5.29984501776126 1104 76-77 -7.047543506189967 1104 78-79 -9.490927721570875 1104 80-81 -5.328480196615455 1104 82-83 -2.8919771644148504 1104 84-85 -2.440752401578301 1104 86-87 -2.3912621420846847 1104 88-89 -2.3763724811174036 1104 90-91 -4.8081875082397225 1104 92-93 -6.945319740368518 1104 94-95 -7.780088481389422 1104 96-97 -7.763324194597454 1104 98-99 -7.929869900950557 1104 100-101 -8.084396654886831 1105 1 0.22668982414143812 1105 2 0.24533977639888604 1105 3 0.2712467594638568 1105 4 0.17334597506854266 1105 5 0.1597856799257258 1105 6 0.06495593936935506 1105 7 0.17908783901892633 1105 8 0.12891425915097443 1105 9 0.35572414597189805 1105 10-11 0.2998466384679901 1105 12-13 0.16205085727712998 1105 14-15 0.2939839665430384 1105 16-17 0.30304247794803274 1105 18-19 0.38572706947388724 1105 20-21 0.32625919661983716 1105 22-23 0.35976044191200174 1105 24-25 0.33767387199255694 1105 26-27 0.39191841545272155 1105 28-29 0.403840888004936 1105 30-31 0.23539854687274442 1105 32-33 0.32040941964751113 1105 34-35 0.3752029260525589 1105 36-37 0.3914289525933228 1105 38-39 0.18913422439948846 1105 40-41 0.06618234986798655 1105 42-43 0.2576809826623858 1105 44-45 0.3067728191952668 1105 46-47 0.41836135564913945 1105 48-49 0.2050443799685837 1105 50-51 0.24105719500796852 1105 52-53 0.4099785235837956 1105 54-55 0.23648694920287738 1105 56-57 1.306943793978796 1105 58-59 -8.554781728561618 1105 60-61 -9.941044614051759 1105 62-63 -3.96310091506583 1105 64-65 -5.093619349093288 1105 66-67 -6.297154116617403 1105 68-69 -3.903482174074526 1105 70-71 -3.9643756347623356 1105 72-73 -2.215552429057766 1105 74-75 -4.38953519725202 1105 76-77 -6.411372019916566 1105 78-79 -9.071633287930009 1105 80-81 -4.7104929202410055 1105 82-83 -2.199612929064102 1105 84-85 -1.6980293493158491 1105 86-87 -1.6796014580649334 1105 88-89 -1.7658409507517838 1105 90-91 -4.17354616621963 1105 92-93 -6.696209190816422 1105 94-95 -7.990165239771088 1105 96-97 -7.758672601476476 1105 98-99 -8.147122997566331 1105 100-101 -8.06720619442736 1106 1 0.18171321365668547 1106 2 0.1554508305777027 1106 3 0.13125578816075034 1106 4 0.12796409921192264 1106 5 -0.0934412041056163 1106 6 0.05590672271140562 1106 7 0.12441744982535852 1106 8 0.025931892268424406 1106 9 0.04394199828873013 1106 10-11 0.22799818780082148 1106 12-13 0.193976745918782 1106 14-15 0.15215632533234924 1106 16-17 0.15736209985324479 1106 18-19 0.09876267307787856 1106 20-21 -0.10588525770226198 1106 22-23 0.26754748174871423 1106 24-25 0.32521037756095694 1106 26-27 0.15974963917309282 1106 28-29 0.23445912440350014 1106 30-31 0.2883780168471972 1106 32-33 0.22486018699621724 1106 34-35 0.3242810152278395 1106 36-37 0.20813358082647682 1106 38-39 0.15565139853806187 1106 40-41 0.17576514078545813 1106 42-43 -0.078721262682798 1106 44-45 0.036466549622282685 1106 46-47 -0.04037324471937609 1106 48-49 0.061664780598903235 1106 50-51 -0.09240438686076402 1106 52-53 0.05369700084819584 1106 54-55 -0.20017758702929456 1106 56-57 1.004916229898278 1106 58-59 -9.078730558301277 1106 60-61 -10.076338369585304 1106 62-63 -3.8973602631770135 1106 64-65 -5.499359240543733 1106 66-67 -7.002754775422584 1106 68-69 -4.1838058613074285 1106 70-71 -4.324643272858848 1106 72-73 -4.638380004484501 1106 74-75 -5.13405294956517 1106 76-77 -6.598617295693163 1106 78-79 -9.337794112196926 1106 80-81 -5.035255781155019 1106 82-83 -2.6830542325761826 1106 84-85 -2.2664445112499934 1106 86-87 -2.0444626569382827 1106 88-89 -1.9452570306436776 1106 90-91 -4.588402717969576 1106 92-93 -6.809528842294238 1106 94-95 -7.497828165201655 1106 96-97 -7.293556203105746 1106 98-99 -6.828227733467894 1106 100-101 -7.420949754087673 1107 1 0.041773179845215225 1107 2 -0.013115389796283239 1107 3 0.016913777174366373 1107 4 0.04363269528660396 1107 5 -0.020723127144712805 1107 6 -0.08650829201672394 1107 7 0.07416444917441822 1107 8 0.17257275544436368 1107 9 0.06796886482707976 1107 10-11 0.045175948563588975 1107 12-13 0.1252131944031163 1107 14-15 -0.015412995206951052 1107 16-17 0.13377309654234182 1107 18-19 0.10396405503978912 1107 20-21 0.09926332310575248 1107 22-23 0.3171395899079599 1107 24-25 0.24977438754518033 1107 26-27 0.17915874625992245 1107 28-29 0.19782934829912335 1107 30-31 0.21743422256330547 1107 32-33 0.14018282429092466 1107 34-35 0.16666137554009453 1107 36-37 -0.01727641837741345 1107 38-39 0.026674921393585294 1107 40-41 0.042887407652557386 1107 42-43 -0.008908419943601587 1107 44-45 -0.0689507785222574 1107 46-47 -0.09718394965575783 1107 48-49 -0.3555085312861621 1107 50-51 -0.27130634530356446 1107 52-53 -0.4710141733277098 1107 54-55 -0.36525553604983685 1107 56-57 0.865102637874422 1107 58-59 -9.15969135198106 1107 60-61 -10.536885604959036 1107 62-63 -4.490419955408335 1107 64-65 -6.2186739845927725 1107 66-67 -7.756983258074303 1107 68-69 -4.600794193540251 1107 70-71 -4.222550310046783 1107 72-73 -2.564369118783194 1107 74-75 -4.740545910972326 1107 76-77 -6.852033565473107 1107 78-79 -7.820115969767276 1107 80-81 -3.0644857857912164 1107 82-83 -1.8768040276936553 1107 84-85 -1.7646889606896714 1107 86-87 -1.694385738184156 1107 88-89 -1.473902827390848 1107 90-91 -1.0681667635448129 1107 92-93 -3.8825472185007044 1107 94-95 -6.345435371446943 1107 96-97 -6.706207464947956 1107 98-99 -6.447463802626869 1107 100-101 -6.981892646888452 1108 1 -0.13424756948442962 1108 2 -0.13079829216142613 1108 3 -0.09008101376092625 1108 4 -0.1318464183031054 1108 5 -0.07466269129511005 1108 6 -0.19965248748704312 1108 7 -0.11965338067172127 1108 8 -0.1812039836410051 1108 9 -0.22426618502346685 1108 10-11 -0.1677876180743212 1108 12-13 -0.06338345079873164 1108 14-15 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88-89 1.800326263413151 2316 90-91 2.8740331092949063 2316 92-93 4.823936510763499 2316 94-95 11.728267015698307 2316 96-97 13.526633122301336 2316 98-99 13.373933804447868 2316 100-101 12.149422017003808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 25.0 5 71.0 6 185.0 7 392.0 8 961.0 9 1547.0 10 2660.0 11 4376.0 12 6499.0 13 9133.0 14 11297.0 15 15481.0 16 21348.0 17 28751.0 18 30655.0 19 30208.0 20 31489.0 21 31740.0 22 31640.0 23 26826.0 24 27081.0 25 31359.0 26 44008.0 27 66611.0 28 94857.0 29 128265.0 30 159476.0 31 172553.0 32 172829.0 33 156329.0 34 138717.0 35 123682.0 36 99673.0 37 63144.0 38 24225.0 39 1764.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05300572740375 19.38448711264304 14.20719130333222 22.355315856620994 2 15.105542002800448 22.194663504895196 39.23476041423378 23.465034078070573 3 20.200182695342946 26.43724526710115 30.559902562483764 22.802669475072143 4 12.374941825286461 16.99891667928399 38.634825939580985 31.991315555848566 5 12.550831317238895 39.399837966433985 34.43336369597669 13.61596702035043 6 29.778134352999412 37.314616113934726 18.105423475599025 14.80182605746684 7 26.699134628438877 34.3613799237597 21.40047277255796 17.539012675243466 8 25.050968271447875 34.3777848175171 20.54218284437874 20.029064066656275 9 26.367378134799964 17.149654136312922 21.06866040884602 35.4143073200411 10-11 24.336361305057864 26.474341389611634 28.020797075983854 21.168500229346648 12-13 26.966109772402085 24.082264212927555 27.853801427703846 21.097824586966517 14-15 23.285175814472172 25.27287817368497 27.77863911360442 23.663306898238435 16-17 21.177551181177147 28.635032658851188 28.32917194406065 21.858244215911014 18-19 20.911409519712382 28.399711710101037 30.04519893958483 20.643679830601748 20-21 22.499632654269842 26.808304751135143 29.00804920142111 21.684013393173906 22-23 21.710363299465683 26.81031667569438 28.884194541526863 22.595125483313076 24-25 22.22171318363688 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28.428922031338494 22.322516588704996 50-51 22.1516558728866 27.57923611585013 28.74441059695999 21.52469741430328 52-53 22.051857214400698 27.801522435695787 28.473876076094385 21.672744273809133 54-55 21.1483856104552 27.22439181504333 29.04097846399538 22.586244110506083 56-57 21.22174534085405 27.79104841600838 28.882181456919405 22.105024786218166 58-59 20.937670405982452 26.878478907766667 30.03897143126406 22.14487925498682 60-61 21.801111709287433 26.900073381911994 29.27848421558724 22.02033069321334 62-63 22.631337369791265 26.884592186540118 28.516952313159706 21.96711813050891 64-65 21.68520429240176 27.452933860015033 29.041481637695536 21.82038020988767 66-67 21.251601362585507 27.030003818496954 29.430360553246395 22.288034265671143 68-69 21.554465980330132 27.10220279519181 29.438689825731867 21.90464139874619 70-71 21.420613085469732 27.110598559338783 29.117049126823236 22.35173922836825 72-73 20.7376354610279 26.744965230067884 29.73054378326465 22.786855525639563 74-75 21.1558655224192 27.478784790036975 29.069654753312967 22.295694934230852 76-77 19.76192318276125 27.134431726496196 29.9226975842322 23.180947506510353 78-79 20.32949259955831 27.16934903242782 29.694436195566926 22.806722172446943 80-81 21.367768861842634 27.93546426947298 28.215389663695422 22.481377204988963 82-83 21.57244524996899 27.674954828919773 27.888602585003756 22.863997336107477 84-85 21.54021585973475 27.256649380456416 27.763279485726866 23.43985527408197 86-87 21.68023103470046 27.414922164346837 27.984435662881086 22.920411138071618 88-89 22.0309135132855 26.845942807402462 27.88699926726297 23.236144412049068 90-91 20.917892687451374 26.98799081205545 28.78390799061042 23.310208509882756 92-93 19.893118834264353 27.92505360045865 29.213035317401104 22.968792247875896 94-95 19.19972497298333 27.881091864097655 29.19924321960628 23.719939943312735 96-97 19.075658301538517 27.65516289785505 28.746043778369145 24.523135022237284 98-99 19.485500746113196 27.668144721869126 28.343090198212444 24.503264333805234 100-101 20.145933771382836 27.428464418208105 28.343709228991138 24.081892581417925 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 646.0 1 495.0 2 345.0 3 362.5 4 406.0 5 490.5 6 649.0 7 911.5 8 1230.5 9 1508.5 10 1656.0 11 1744.5 12 1925.5 13 2160.5 14 2377.0 15 2647.0 16 2887.0 17 3056.5 18 3324.0 19 3693.0 20 4140.0 21 4672.0 22 5418.0 23 6356.5 24 7600.5 25 9136.5 26 11241.0 27 13853.0 28 16540.0 29 19438.5 30 22536.5 31 26022.5 32 29706.0 33 33380.0 34 37142.0 35 40842.0 36 44352.5 37 47495.0 38 49647.0 39 51668.5 40 53969.0 41 56294.0 42 58999.5 43 61962.0 44 65985.0 45 71696.0 46 78344.5 47 85710.5 48 92692.5 49 89364.0 50 77760.5 51 68407.0 52 61353.5 53 55748.5 54 50252.5 55 44733.0 56 39317.5 57 34046.0 58 29150.5 59 24360.0 60 19837.5 61 15616.0 62 11906.5 63 8828.0 64 6281.0 65 4435.5 66 3045.0 67 2036.0 68 1431.0 69 972.5 70 634.5 71 442.0 72 301.0 73 217.0 74 150.5 75 94.0 76 53.0 77 24.0 78 12.5 79 7.5 80 5.0 81 3.5 82 2.5 83 1.0 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022348029211108983 2 0.008548121173249185 3 1.1174014605554492E-4 4 0.0 5 0.14431239863073625 6 0.0 7 0.0 8 0.0015643620447776288 9 0.0 10-11 0.0 12-13 0.0 14-15 6.425058398193832E-4 16-17 0.0 18-19 1.1174014605554492E-4 20-21 0.0 22-23 4.1902554770829344E-4 24-25 2.793503651388623E-5 26-27 3.072854016527485E-4 28-29 0.0034080744546941197 30-31 0.013520557672720934 32-33 0.002849373724416395 34-35 0.005000371535985635 36-37 0.11539963583886399 38-39 0.11548344094840567 40-41 0.12651778037139072 42-43 0.12897606358461272 44-45 0.12520483365523807 46-47 0.11900325554915533 48-49 0.11897532051264144 50-51 0.11782998401557211 52-53 0.09752121246997682 54-55 0.08453142049101972 56-57 0.5726123784616399 58-59 1.5894197725305845 60-61 0.5282794755141025 62-63 0.005391462047180042 64-65 0.12271861540550219 66-67 1.8962582135991108 68-69 2.8587040266120334 70-71 2.7686414688912637 72-73 2.6168424804748063 74-75 1.9946174771645044 76-77 1.4281787417724334 78-79 0.9441204290598129 80-81 0.05573039784520302 82-83 2.793503651388623E-4 84-85 5.587007302777246E-5 86-87 3.352204381666347E-4 88-89 1.3967518256943114E-4 90-91 0.2505214074565317 92-93 0.4041082382098782 94-95 2.003361143593351 96-97 2.4164365285241867 98-99 1.4372296936029325 100-101 0.9502382020563539 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1789867.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.54165370997204 #Duplication Level Percentage of deduplicated Percentage of total 1 73.20856498924786 37.73290505279795 2 14.384179473263863 14.827687946261081 3 5.377584437631762 8.315087846416528 4 2.529032003669514 5.214019670182831 5 1.3414232715686547 3.4569586870844695 6 0.8041819766299131 2.4869321375555877 7 0.5227666845478827 1.8860981598254007 8 0.34283340557793 1.413616053640645 9 0.2563045989295382 1.1889326594069576 >10 1.1292611092904246 10.097733816922688 >50 0.06534931333584168 2.3272855042741436 >100 0.032099589261769326 3.021246828719707 >500 0.0018495847417892635 0.6910351042337322 >1k 0.0038079685860367184 4.181987659931414 >5k 6.527946147491517E-4 2.5558709714164607 >10k+ 1.0879910245819195E-4 0.6026019013304947 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10746 0.6003798047564428 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9707 0.5423307988805872 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9633 0.5381964134765321 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8408 0.4697555740175108 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6700 0.37432948928607546 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5960 0.3329856352455238 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5170 0.2888482775535836 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4776 0.26683546878064124 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4438 0.24795138409725417 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4334 0.2421408965023658 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4108 0.22951425999808922 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3894 0.2175580643701459 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3658 0.20437272713559165 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3533 0.19738896800712008 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2733 0.15269290958490211 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2666 0.14894961469204138 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2491 0.1391723519121812 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2320 0.1296185694244321 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2303 0.12866877818295996 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2204 0.12313764095321048 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1953 0.1091142526232396 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1935 0.1081085913087397 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1921 0.1073264102863509 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.7935036513886227E-5 0.0 12-13 0.0 0.0 0.0 5.5870073027772454E-5 0.0 14-15 0.0 0.0 0.0 1.3967518256943114E-4 0.0 16-17 0.0 0.0 0.0 1.955452555972036E-4 0.0 18-19 0.0 0.0 0.0 3.072854016527485E-4 0.0 20-21 0.0 0.0 0.0 5.028306572499521E-4 0.0 22-23 0.0 0.0 0.0 0.0011453364970693352 0.0 24-25 0.0 0.0 0.0 0.002095127738541467 0.0 26-27 0.0 0.0 0.0 0.0025141532862497607 0.0 28-29 0.0 0.0 0.0 0.005503202193235587 0.0 30-31 0.0 0.0 0.0 0.013995453293457 0.0 32-33 0.0 0.0 0.0 0.03125930585903869 0.0 34-35 0.0 0.0 0.0 0.05941782266503601 0.0 36-37 5.5870073027772454E-5 0.0 0.0 0.10372279057605957 0.0 38-39 5.5870073027772454E-5 0.0 0.0 0.1649005205414704 0.0 40-41 5.5870073027772454E-5 0.0 0.0 0.2320004782478251 0.0 42-43 8.380510954165868E-5 0.0 0.0 0.30133523887529073 0.0 44-45 1.1174014605554491E-4 0.0 0.0 0.38245858491161633 0.0 46-47 2.2348029211108981E-4 0.0 0.0 0.4762644375252463 0.0 48-49 2.2348029211108981E-4 8.380510954165868E-5 0.0 0.5622205448784742 0.0 50-51 2.2348029211108981E-4 1.3967518256943114E-4 0.0 0.6455786938359107 0.0 52-53 2.2348029211108981E-4 1.6761021908331735E-4 0.0 0.7270093252738891 0.0 54-55 2.2348029211108981E-4 1.6761021908331735E-4 0.0 0.8142504443067557 0.0 56-57 2.2348029211108981E-4 1.6761021908331735E-4 0.0 0.8881386158859848 2.7935036513886227E-5 58-59 2.2348029211108981E-4 1.6761021908331735E-4 0.0 0.9415224706640214 5.5870073027772454E-5 60-61 2.2348029211108981E-4 2.2348029211108981E-4 0.0 0.9883415918612947 5.5870073027772454E-5 62-63 2.2348029211108981E-4 2.2348029211108981E-4 0.0 1.0282607590396382 5.5870073027772454E-5 64-65 2.2348029211108981E-4 2.5141532862497603E-4 0.0 1.0622297634405238 5.5870073027772454E-5 66-67 2.2348029211108981E-4 3.072854016527485E-4 5.5870073027772454E-5 1.0916732919261598 5.5870073027772454E-5 68-69 2.2348029211108981E-4 3.6315547468052095E-4 5.5870073027772454E-5 1.1330730160397393 5.5870073027772454E-5 70-71 2.2348029211108981E-4 3.910905111944072E-4 5.5870073027772454E-5 1.204614644551802 5.5870073027772454E-5 72-73 2.2348029211108981E-4 3.910905111944072E-4 5.5870073027772454E-5 1.3058791519146395 5.5870073027772454E-5 74-75 2.2348029211108981E-4 3.910905111944072E-4 5.5870073027772454E-5 1.4341009695133773 5.5870073027772454E-5 76-77 2.2348029211108981E-4 3.910905111944072E-4 5.5870073027772454E-5 1.6317134178126085 5.5870073027772454E-5 78-79 2.2348029211108981E-4 3.910905111944072E-4 5.5870073027772454E-5 1.9459546435573145 5.5870073027772454E-5 80-81 2.2348029211108981E-4 3.910905111944072E-4 5.5870073027772454E-5 2.3340002357717085 5.5870073027772454E-5 82-83 2.2348029211108981E-4 3.910905111944072E-4 5.5870073027772454E-5 2.7019046666595896 5.5870073027772454E-5 84-85 2.2348029211108981E-4 4.190255477082934E-4 5.5870073027772454E-5 3.0084078872899496 5.5870073027772454E-5 86-87 2.2348029211108981E-4 4.4696058422217963E-4 5.5870073027772454E-5 3.2401848852456636 5.5870073027772454E-5 88-89 2.2348029211108981E-4 5.028306572499521E-4 5.5870073027772454E-5 3.4412612780726164 5.5870073027772454E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9635 0.0 27.496143 1 TACATGG 9740 0.0 27.24194 2 ACATGGG 9490 0.0 25.925615 3 CATGGGG 3115 0.0 23.559177 4 CATGGGA 6625 0.0 22.296534 4 GAGTACT 6605 0.0 20.76693 12-13 CGCGAGA 100 1.0478139E-5 19.456432 68-69 ATGGGAG 2505 0.0 19.15517 5 AGTACTT 6850 0.0 18.755514 12-13 GTACTTT 7260 0.0 18.73157 14-15 TACGCGG 135 3.7898826E-7 18.021992 64-65 ATGGGAT 2980 0.0 17.364809 5 TTACGCG 115 3.507389E-5 16.907633 62-63 GCTTACG 430 0.0 16.763432 60-61 AGTACAT 7140 0.0 16.736065 2 ATGGGGA 1760 0.0 16.57191 5 TACACTG 1400 0.0 16.464994 5 ACTTTTT 8435 0.0 16.372858 16-17 TACTTTT 8105 0.0 15.880341 14-15 ATGGGGG 1640 0.0 15.489717 5 >>END_MODULE