FastQCFastQC Report
Fri 27 May 2016
SRR939680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707164
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45570.2669339325337226No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37710.22089266174778757No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37560.2200140115419491No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33300.19506034569613698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23580.1381238123578051No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19610.11486887024328067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17240.10098619699103308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG76850.031.0469741
TACATGG90550.026.1302342
ACATGGG90950.024.8217283
GTATAAT7550.023.9007631
CATGGGA68300.022.1948834
GAGTACT60500.021.8773912-13
GTATAGC5452.6466296E-920.2340321
ATGGGAG26000.019.9626525
GTACTTT67150.019.7842914-15
AGTACTT65850.019.67001212-13
TACCTGG11550.019.2180772
CATGGGG28800.019.021034
GTTCTAA10600.018.9151731
GGATCGT1550.001006580318.2654866
GGTTAGT3350.001128496617.95531
TAGCCCT11300.017.5525154
GTATATG8602.910383E-1117.4855381
GTATAAG13850.017.3719141
ATGGGAT31250.017.2128895
GCCTTAG7001.2862074E-817.1857851
Produced by FastQC (version 0.11.5)