##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1707164 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.381508162074645 2.0 2.0 31.0 2.0 33.0 2 22.666883205128507 16.0 16.0 31.0 16.0 34.0 3 27.190965249970127 28.0 25.0 31.0 16.0 34.0 4 32.409354344398075 35.0 32.0 35.0 26.0 37.0 5 33.01829876918679 35.0 33.0 35.0 28.0 37.0 6 33.45455621135404 35.0 33.0 37.0 28.0 37.0 7 33.6387406248023 35.0 33.0 37.0 28.0 37.0 8 33.57240897769634 35.0 33.0 37.0 28.0 37.0 9 34.91533853806664 37.0 34.0 39.0 28.0 39.0 10-11 34.76197131617115 37.0 34.0 39.0 27.0 39.0 12-13 34.77470383630395 37.0 34.0 39.0 27.0 39.0 14-15 35.42310346281904 38.0 34.0 40.0 27.0 41.0 16-17 35.08676670782655 38.0 33.0 40.0 26.0 41.0 18-19 35.401737911530475 38.0 34.0 40.0 27.0 41.0 20-21 35.406315093336076 38.0 34.0 40.0 27.0 41.0 22-23 35.24068103591688 38.0 34.0 40.0 26.0 41.0 24-25 35.049191817540674 38.0 34.0 40.0 25.0 41.0 26-27 35.11373658301136 38.0 34.0 40.0 25.5 41.0 28-29 34.873706626897004 38.0 34.0 40.0 25.0 41.0 30-31 34.743652630913026 38.0 33.5 40.0 24.5 41.0 32-33 34.43820306660637 38.0 33.0 40.0 24.0 41.0 34-35 34.16814231087347 38.0 33.0 40.0 24.0 41.0 36-37 33.86864384441097 38.0 33.0 40.0 21.5 41.0 38-39 33.64066838335391 38.0 32.0 40.0 20.5 41.0 40-41 33.27474191114621 37.0 31.5 40.0 18.0 41.0 42-43 33.02938792055127 37.0 31.0 40.0 17.0 41.0 44-45 32.66943451244286 37.0 31.0 40.0 15.0 41.0 46-47 32.29592938932639 37.0 30.5 39.5 12.5 41.0 48-49 31.897192068248863 37.0 30.0 39.0 9.0 40.0 50-51 31.21337112310241 35.5 29.5 38.5 8.5 40.0 52-53 31.243391086035086 36.0 30.0 39.0 8.0 40.0 54-55 31.38065323542436 36.0 30.0 39.0 7.0 40.0 56-57 31.106696544678776 36.0 29.0 39.0 4.0 40.0 58-59 30.718833105665304 35.5 28.0 39.0 2.0 40.0 60-61 30.11627881094025 35.0 28.0 38.0 2.0 40.0 62-63 29.69210837388792 34.0 27.5 38.0 2.0 40.0 64-65 29.09480782162698 34.0 26.0 38.0 2.0 40.0 66-67 28.485247755927375 34.0 26.0 37.0 2.0 39.0 68-69 28.156825296222273 33.5 26.0 37.0 2.0 39.0 70-71 27.620916326726665 33.0 25.0 36.0 2.0 39.0 72-73 27.01195637911765 32.0 23.5 36.0 2.0 38.5 74-75 26.441311438151224 32.0 22.0 35.0 2.0 38.0 76-77 25.785038520025026 31.5 20.5 35.0 2.0 37.0 78-79 25.11933680653997 31.0 19.0 34.5 2.0 36.5 80-81 24.368820453102337 30.5 15.0 34.0 2.0 36.0 82-83 23.6825181997746 30.0 9.0 34.0 2.0 35.5 84-85 22.991585166978687 29.5 4.5 34.0 2.0 35.0 86-87 22.274815717763495 29.0 2.0 34.0 2.0 35.0 88-89 21.413807343641267 29.0 2.0 33.5 2.0 35.0 90-91 20.39557535187012 26.5 2.0 33.0 2.0 34.0 92-93 19.329288515924656 25.5 2.0 32.0 2.0 34.0 94-95 18.327010175940917 24.5 2.0 32.0 2.0 34.0 96-97 17.41027253386318 21.5 2.0 31.5 2.0 34.0 98-99 16.349610816535495 17.0 2.0 31.0 2.0 34.0 100-101 14.78329234918262 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.802180089202512 1101 2 7.927865964505795 1101 3 3.6753029495906233 1101 4 2.2865071844816924 1101 5 1.5327677857127213 1101 6 1.0600395158950917 1101 7 0.7109033422356816 1101 8 0.7542184674907091 1101 9 0.6992719444838897 1101 10-11 0.6490064519827996 1101 12-13 0.3412011151437042 1101 14-15 0.6468045391372144 1101 16-17 0.8865325011812288 1101 18-19 0.8793536892565257 1101 20-21 0.7852851330117403 1101 22-23 0.5644691234390962 1101 24-25 0.7727563474898602 1101 26-27 0.9579693723154037 1101 28-29 0.9272051731879216 1101 30-31 0.9853785110818833 1101 32-33 0.7879993966348877 1101 34-35 0.6800490769796426 1101 36-37 0.5851826279068959 1101 38-39 0.5230551020565599 1101 40-41 0.7387418653584206 1101 42-43 0.798662852893699 1101 44-45 0.6199839327789505 1101 46-47 0.25907271716264546 1101 48-49 0.3024858746907597 1101 50-51 0.1972709232408043 1101 52-53 0.316059714527114 1101 54-55 0.5304703674770117 1101 56-57 0.364540308835025 1101 58-59 0.5589613374473608 1101 60-61 0.35842673880957676 1101 62-63 0.12896439903251888 1101 64-65 0.16359377534604036 1101 66-67 -0.05591249592003322 1101 68-69 -0.2551883842184921 1101 70-71 -0.4768090958384974 1101 72-73 -0.3367528950889209 1101 74-75 -0.4065549711112979 1101 76-77 -0.5692051498869866 1101 78-79 -0.8797015873808043 1101 80-81 -1.0078096768536824 1101 82-83 -0.7748990673362037 1101 84-85 -0.9832938934962243 1101 86-87 -1.1467442885954888 1101 88-89 -0.9437233843993909 1101 90-91 -0.8902242401464875 1101 92-93 -0.6859712740397939 1101 94-95 -0.7015984826766086 1101 96-97 -1.3119614913260271 1101 98-99 -1.5741839067850005 1101 100-101 -1.361503020831762 1102 1 14.8139347459046 1102 2 7.871116735676651 1102 3 3.6929155801047315 1102 4 2.3757290287886192 1102 5 1.6233281039185457 1102 6 1.2668173894419894 1102 7 1.0961309258085805 1102 8 0.9275938474848289 1102 9 0.9743782224081912 1102 10-11 0.9742177605388775 1102 12-13 0.6259339106789099 1102 14-15 0.8074842017316826 1102 16-17 0.8831166400934833 1102 18-19 1.037041140117502 1102 20-21 0.8740811774600843 1102 22-23 0.6830548141552839 1102 24-25 0.7131327545648389 1102 26-27 0.9308618350557651 1102 28-29 0.9101525340029823 1102 30-31 1.085372661710089 1102 32-33 0.9511400963917964 1102 34-35 0.8812055321915224 1102 36-37 0.7479577419266334 1102 38-39 0.5998870306804562 1102 40-41 0.7839876163919541 1102 42-43 0.8778986152646908 1102 44-45 0.9095407396413009 1102 46-47 0.7793489623476688 1102 48-49 0.5014999974828385 1102 50-51 0.5826800093796862 1102 52-53 0.6380990291871846 1102 54-55 0.650237545275548 1102 56-57 0.547338768729837 1102 58-59 0.47962837228037003 1102 60-61 0.6851477014097291 1102 62-63 0.24809330714404965 1102 64-65 0.1939791113150342 1102 66-67 0.2548500041014705 1102 68-69 -0.10941644778395698 1102 70-71 0.23840477611875244 1102 72-73 0.07263130369337745 1102 74-75 -0.08658487172358775 1102 76-77 -0.4453378205209013 1102 78-79 -0.5474521082329566 1102 80-81 -0.374791091335009 1102 82-83 -0.28532256185414084 1102 84-85 -0.46977238243144726 1102 86-87 -0.48971317165795014 1102 88-89 -0.4709281244550283 1102 90-91 -0.41806036827525617 1102 92-93 -0.3430669061074312 1102 94-95 -0.1127783525796957 1102 96-97 -0.9876886628622934 1102 98-99 -1.264764611771934 1102 100-101 -0.9646222843614431 1103 1 14.673090543295746 1103 2 7.726997493039686 1103 3 3.6983199119957035 1103 4 1.9743764269401183 1103 5 1.4617549727733703 1103 6 1.0844044231722094 1103 7 0.7621424698374923 1103 8 0.7310152154301832 1103 9 0.9003384742893772 1103 10-11 0.8611685544093888 1103 12-13 0.7407129156891941 1103 14-15 0.8599468868231668 1103 16-17 0.8597722361619518 1103 18-19 0.6973715156552416 1103 20-21 0.5610322865689525 1103 22-23 0.7212512437655647 1103 24-25 1.0168312456907387 1103 26-27 0.9991827293698137 1103 28-29 0.9404400629153571 1103 30-31 0.8869834151211862 1103 32-33 0.8907328313511229 1103 34-35 0.9056269050087522 1103 36-37 1.125930117302154 1103 38-39 1.0320890021811948 1103 40-41 0.819096142930583 1103 42-43 0.9431177073610044 1103 44-45 0.7441013532441758 1103 46-47 0.9125089432384925 1103 48-49 1.0035137974721096 1103 50-51 1.0553908271853771 1103 52-53 1.266227805778616 1103 54-55 1.093734937251238 1103 56-57 1.0675666115565718 1103 58-59 0.7765522476603373 1103 60-61 0.6190304665545661 1103 62-63 0.6036389088094261 1103 64-65 0.29790732523464314 1103 66-67 0.47065688678666007 1103 68-69 0.5295728636294186 1103 70-71 0.3739737512759653 1103 72-73 0.3345806288396851 1103 74-75 0.4059828106697445 1103 76-77 0.3707177279179099 1103 78-79 0.06798173283474895 1103 80-81 -0.02644711010945855 1103 82-83 -0.10956814629603784 1103 84-85 -0.06037446764725729 1103 86-87 -0.2798470992442539 1103 88-89 -0.253194132330826 1103 90-91 0.016752063464888778 1103 92-93 0.33132711261715997 1103 94-95 0.22412992709863389 1103 96-97 -0.20982510203862859 1103 98-99 -0.5905551507808191 1103 100-101 -0.37448818482693724 1104 1 14.795670494072787 1104 2 7.9253174602618515 1104 3 3.7366391260919727 1104 4 2.3201247221497354 1104 5 1.5782888401141761 1104 6 1.1012626404782537 1104 7 0.7563063061933519 1104 8 0.8099273531780327 1104 9 0.7541766904825948 1104 10-11 0.6126821126425952 1104 12-13 0.5295753358873583 1104 14-15 0.7076495156259668 1104 16-17 0.8556003964383834 1104 18-19 0.6829062996739097 1104 20-21 0.5991872461558785 1104 22-23 0.7685555588959119 1104 24-25 0.7877161573754847 1104 26-27 0.8020478113621792 1104 28-29 0.8835963404726002 1104 30-31 0.7974741216038552 1104 32-33 0.7437354431242866 1104 34-35 0.6384185403654214 1104 36-37 0.6943235030582997 1104 38-39 0.5887170439808074 1104 40-41 0.6885621429496851 1104 42-43 0.5383735200597215 1104 44-45 0.4854555803880771 1104 46-47 0.4443389098930055 1104 48-49 0.346434076848098 1104 50-51 0.4104894051825241 1104 52-53 0.36409384603937767 1104 54-55 0.4499866690192711 1104 56-57 0.3739864196668421 1104 58-59 0.35024631892965274 1104 60-61 0.17447416168245766 1104 62-63 0.039086324604049594 1104 64-65 0.37059057790669314 1104 66-67 0.21858986532650704 1104 68-69 0.17685253355794472 1104 70-71 -0.1334391994544255 1104 72-73 -0.28497557767643045 1104 74-75 -0.313259303006177 1104 76-77 -0.43466133327132184 1104 78-79 -0.4736206043284703 1104 80-81 -0.48647888752590873 1104 82-83 -0.3024892125199692 1104 84-85 -0.39744776421980177 1104 86-87 -0.4156938684746798 1104 88-89 -0.06375247963539721 1104 90-91 -0.08645175656949533 1104 92-93 0.014891011134004373 1104 94-95 -0.10144232212308779 1104 96-97 -0.4297235312091061 1104 98-99 -1.025445450125881 1104 100-101 -1.0310896143048467 1105 1 14.739223126102445 1105 2 8.006538551209971 1105 3 3.735022902426259 1105 4 2.153725618237573 1105 5 1.5960244827843084 1105 6 0.9426837526639318 1105 7 0.8944851515325638 1105 8 0.8918535081414021 1105 9 0.8712230260336895 1105 10-11 0.9599498325854654 1105 12-13 0.7512234843949628 1105 14-15 0.8270388331354113 1105 16-17 0.9738119430462504 1105 18-19 0.9706486333159106 1105 20-21 0.976820389074355 1105 22-23 0.756013126602042 1105 24-25 0.9170657172671071 1105 26-27 1.0254506272793762 1105 28-29 1.0807051212884673 1105 30-31 0.968350239287318 1105 32-33 0.6544340902006311 1105 34-35 0.6962043881606519 1105 36-37 0.8204297684797908 1105 38-39 0.839292227782714 1105 40-41 0.8970116072723116 1105 42-43 0.8038195601119256 1105 44-45 0.7696780175231908 1105 46-47 0.5827766593058286 1105 48-49 0.4725426258777361 1105 50-51 0.5704612939662432 1105 52-53 0.5598099113368704 1105 54-55 0.3927017877611867 1105 56-57 0.6942992854009589 1105 58-59 0.658100389518264 1105 60-61 0.6724525975707323 1105 62-63 0.29458753662907355 1105 64-65 0.3679598613218751 1105 66-67 0.28307496307064284 1105 68-69 0.18894709320407443 1105 70-71 0.3167033547841207 1105 72-73 0.26872518059565564 1105 74-75 0.17955760756887074 1105 76-77 0.007014458312898597 1105 78-79 0.028843979763685468 1105 80-81 0.061173163668019725 1105 82-83 0.2314452045376676 1105 84-85 0.3866539271345708 1105 86-87 0.08888276770201387 1105 88-89 0.03826400344820513 1105 90-91 0.26614504843434617 1105 92-93 0.30897406824590234 1105 94-95 0.4350291835853639 1105 96-97 -0.04280038489947202 1105 98-99 -0.42438893881688244 1105 100-101 -0.1173178015652816 1106 1 14.800741050670016 1106 2 7.9485062400140265 1106 3 3.604802360972908 1106 4 2.2733555319594245 1106 5 1.6081301809475548 1106 6 1.1165345170371808 1106 7 0.8563217879335028 1106 8 0.9075173622950601 1106 9 1.1702645120870798 1106 10-11 0.9671270922583091 1106 12-13 0.8955797281143703 1106 14-15 1.0384857016132685 1106 16-17 1.0874127921516603 1106 18-19 1.0773787450434327 1106 20-21 0.8325432287016596 1106 22-23 0.893081215579727 1106 24-25 0.9212470262451475 1106 26-27 0.9339080857903568 1106 28-29 1.0353781740840304 1106 30-31 0.8090567568253917 1106 32-33 0.9549832140404035 1106 34-35 0.9759305100441438 1106 36-37 0.9995431430791726 1106 38-39 1.1759586566047275 1106 40-41 1.2405059614348843 1106 42-43 1.0046193127534906 1106 44-45 0.9504866298780357 1106 46-47 1.02341390458907 1106 48-49 1.267246122788734 1106 50-51 1.0309020795671273 1106 52-53 0.8093942778079075 1106 54-55 0.9380450867854258 1106 56-57 0.9056310965285235 1106 58-59 0.9946333280294759 1106 60-61 1.077990810287094 1106 62-63 1.176602021368364 1106 64-65 0.9750788490550342 1106 66-67 0.7483053453464592 1106 68-69 0.9724766483751139 1106 70-71 0.9858593630373313 1106 72-73 1.0060510337146908 1106 74-75 0.8121393518026245 1106 76-77 0.8203596244176552 1106 78-79 0.9641829381230345 1106 80-81 0.9493811231392861 1106 82-83 0.961045268755722 1106 84-85 0.8023561337383249 1106 86-87 0.7579585033852503 1106 88-89 0.6538984243262078 1106 90-91 0.7973761323518858 1106 92-93 1.199734100830618 1106 94-95 1.181976447479947 1106 96-97 0.5772237563326783 1106 98-99 0.2896653016559547 1106 100-101 0.5706056362413143 1107 1 14.717226498807417 1107 2 8.063503432817022 1107 3 3.618385961063442 1107 4 2.2468282551168883 1107 5 1.2627658773234742 1107 6 1.1114515797978797 1107 7 0.7783352095686098 1107 8 0.8102934008844684 1107 9 0.6945713107948848 1107 10-11 0.7085833661231504 1107 12-13 0.4914325722017736 1107 14-15 0.31864028851438775 1107 16-17 0.8175329872151096 1107 18-19 0.6783450968925067 1107 20-21 0.6517512170143078 1107 22-23 0.46576792357538466 1107 24-25 0.8301448119399026 1107 26-27 0.47269913920940354 1107 28-29 0.8260934067286456 1107 30-31 0.7887273594468169 1107 32-33 0.8250498225054983 1107 34-35 0.7324554059133064 1107 36-37 0.6212079431802877 1107 38-39 0.6063988133374494 1107 40-41 0.7155304325501533 1107 42-43 0.5881300433007866 1107 44-45 0.4588270914341308 1107 46-47 0.16049389059718067 1107 48-49 0.2813810210976513 1107 50-51 0.34855170610704533 1107 52-53 0.30785810905862476 1107 54-55 0.4283699573751143 1107 56-57 0.20916066909970965 1107 58-59 0.373003507660691 1107 60-61 0.4997230713005685 1107 62-63 0.3137297738176805 1107 64-65 0.008217883202899401 1107 66-67 0.2158813380800808 1107 68-69 0.4198816813413089 1107 70-71 0.14590148568979444 1107 72-73 0.12308689822848962 1107 74-75 -0.031383518769427354 1107 76-77 0.07611379038619859 1107 78-79 0.1529647322011698 1107 80-81 0.16759248302782126 1107 82-83 0.08566585623885814 1107 84-85 0.3315883139162814 1107 86-87 0.32260516399660233 1107 88-89 0.3954230409454027 1107 90-91 0.6287250272568983 1107 92-93 0.9632634676386758 1107 94-95 0.8783667742611954 1107 96-97 0.21610710921351028 1107 98-99 0.43170947018183625 1107 100-101 0.6106342079218408 1108 1 14.324107973014229 1108 2 7.570218188442066 1108 3 3.235667388590212 1108 4 -0.3013365196243285 1108 5 0.38465768471488815 1108 6 0.5622733720618243 1108 7 0.43803509289753606 1108 8 0.46234913708649117 1108 9 0.6328468258066664 1108 10-11 0.39812334998835297 1108 12-13 0.04948448749142642 1108 14-15 0.21335719931747832 1108 16-17 0.30153433187899026 1108 18-19 0.1985060559467442 1108 20-21 0.414718935501142 1108 22-23 -0.38629118009232855 1108 24-25 0.29788216819484603 1108 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58-59 0.2836136363928823 2312 60-61 0.38936145222321983 2312 62-63 0.15941639156404008 2312 64-65 0.22526075213660235 2312 66-67 -0.01820867641097834 2312 68-69 0.2423577242483539 2312 70-71 0.3103749038554362 2312 72-73 0.22573779394840088 2312 74-75 0.37784921712637143 2312 76-77 0.467997544191153 2312 78-79 0.48778305279142486 2312 80-81 0.4922084378596985 2312 82-83 0.4536363015692082 2312 84-85 0.5512979631720469 2312 86-87 0.47442937672692054 2312 88-89 0.8571376586487034 2312 90-91 0.7495697564346102 2312 92-93 1.5430533370445545 2312 94-95 1.9264739900890078 2312 96-97 1.7198357722798612 2312 98-99 1.448318480009636 2312 100-101 1.476050316130312 2313 1 -14.208002118728892 2313 2 -7.418055636499362 2313 3 -3.4376187059921435 2313 4 -0.8787352388106342 2313 5 -0.3753479048856434 2313 6 -0.8461282185186505 2313 7 -0.17819147068504293 2313 8 0.07025824643977785 2313 9 -2.4463533117115333 2313 10-11 -0.4290720445550136 2313 12-13 -0.7152835177754184 2313 14-15 -0.9364756681183621 2313 16-17 -0.5691943158177182 2313 18-19 -0.03791913724821683 2313 20-21 -0.99553757220297 2313 22-23 -1.7690868749305508 2313 24-25 -1.1225614741899932 2313 26-27 -0.12052832671579239 2313 28-29 0.317405182709976 2313 30-31 -0.8775289194855205 2313 32-33 -0.09800626550183722 2313 34-35 -0.8324515181485523 2313 36-37 -0.7590813233494984 2313 38-39 -0.5277714584276225 2313 40-41 -0.11712418260717072 2313 42-43 0.1456594586308526 2313 44-45 0.0015958070089183707 2313 46-47 -0.4222970995610922 2313 48-49 0.21533385050010168 2313 50-51 -0.7045152161364783 2313 52-53 0.22083949387099722 2313 54-55 -0.6332885899280676 2313 56-57 -0.22971870721195486 2313 58-59 0.14274000085330485 2313 60-61 -1.3460386967255538 2313 62-63 -0.15237156796080242 2313 64-65 0.2940011681736152 2313 66-67 -0.9665846249925991 2313 68-69 -0.20805132822766836 2313 70-71 0.3142355892893356 2313 72-73 -0.5919499609037118 2313 74-75 0.03295707021905159 2313 76-77 0.41144476493710513 2313 78-79 0.5482533547378985 2313 80-81 0.5715087184716872 2313 82-83 0.42447439331391834 2313 84-85 0.5057032022781911 2313 86-87 0.602475077398477 2313 88-89 0.8068929361639441 2313 90-91 1.189410008581099 2313 92-93 1.7336269070162018 2313 94-95 1.627112881891378 2313 96-97 1.158096593185661 2313 98-99 1.0527403894251073 2313 100-101 1.2803528966004443 2314 1 -14.208002118728892 2314 2 -7.188913943427748 2314 3 -3.1868397700534707 2314 4 -1.038113819693855 2314 5 -0.7643402248295317 2314 6 -5.937929152040642 2314 7 -1.4488161568885687 2314 8 -3.8883152137654236 2314 9 -1.1146166869484588 2314 10-11 -0.15399355039372153 2314 12-13 -2.5607328983501887 2314 14-15 -2.6536828854973393 2314 16-17 -2.0455031323876156 2314 18-19 -0.8641803888787578 2314 20-21 -1.5488811712353439 2314 22-23 -0.6376692115221587 2314 24-25 -2.363410928754668 2314 26-27 -3.9495200581036016 2314 28-29 -1.5320472799767728 2314 30-31 -1.7506935854619385 2314 32-33 -3.7947644626465014 2314 34-35 -2.42390652483887 2314 36-37 -0.7344874870930056 2314 38-39 -0.9679150060514203 2314 40-41 -1.9429815526503873 2314 42-43 -1.0770174264902082 2314 44-45 -2.0873818590900157 2314 46-47 -1.8794288976839582 2314 48-49 -0.980377857922015 2314 50-51 -1.9123806073581022 2314 52-53 -1.067120570798103 2314 54-55 -0.40280402811406546 2314 56-57 -1.0482128204501393 2314 58-59 -1.342933463435756 2314 60-61 -0.5276175524284241 2314 62-63 -2.9447458031381935 2314 64-65 -1.2085407334150062 2314 66-67 -2.1767619004824823 2314 68-69 -2.02223576968796 2314 70-71 -2.5602446196120354 2314 72-73 -2.722584784685221 2314 74-75 -2.3089931043058414 2314 76-77 -1.045553707219387 2314 78-79 -0.5693551617885184 2314 80-81 -0.3429773880017102 2314 82-83 -0.6211936538570484 2314 84-85 -0.2427812658472952 2314 86-87 0.05402219911907835 2314 88-89 0.3012749282700824 2314 90-91 0.4115301677074399 2314 92-93 0.9937086322301809 2314 94-95 1.026912412487885 2314 96-97 1.0080520310833876 2314 98-99 0.9488410253058852 2314 100-101 0.6615483500648764 2315 1 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60-61 -4.766670525481796 2315 62-63 -2.509041367666015 2315 64-65 -3.116214949893074 2315 66-67 -1.8175605993501378 2315 68-69 -1.352101244553367 2315 70-71 -2.0177633796412273 2315 72-73 -2.1111285299345504 2315 74-75 -2.5272827304162213 2315 76-77 -2.241719860635019 2315 78-79 -1.6029814032656695 2315 80-81 -1.2589856836343039 2315 82-83 -0.9289597639100045 2315 84-85 -0.7730555662775167 2315 86-87 -1.2608344645516958 2315 88-89 -1.8052004489158548 2315 90-91 -1.4952550509148104 2315 92-93 -0.48795046907838824 2315 94-95 -0.12617988824234772 2315 96-97 -0.9962618617376933 2315 98-99 -0.6560673395305123 2315 100-101 -0.18439487634500829 2316 1 -14.208002118728892 2316 2 -7.465635990417743 2316 3 -5.07796720725614 2316 4 -3.0167814648850317 2316 5 -2.1643511551565027 2316 6 -1.4541997244774798 2316 7 -4.583391904054494 2316 8 -2.9741169792734787 2316 9 -1.2763009462366384 2316 10-11 -4.582376486591848 2316 12-13 -3.8007836983460273 2316 14-15 -1.808744537746385 2316 16-17 -2.9938963743225955 2316 18-19 -2.137232968956411 2316 20-21 -3.0400256545431716 2316 22-23 -2.8389225556817195 2316 24-25 -3.594279603478551 2316 26-27 -4.25631742025136 2316 28-29 -3.6296876305800225 2316 30-31 -2.6721975724288214 2316 32-33 -3.002868128990464 2316 34-35 -3.9680826124064055 2316 36-37 -2.1426584269970235 2316 38-39 -3.2083646328588245 2316 40-41 -2.9645871656335316 2316 42-43 -6.539921978711149 2316 44-45 -3.4247991009000742 2316 46-47 -4.904307753226675 2316 48-49 -4.407725015516512 2316 50-51 -3.2870585530812235 2316 52-53 -4.482731289805422 2316 54-55 -4.555575516616404 2316 56-57 -4.666044998291767 2316 58-59 -3.495757651728855 2316 60-61 -3.1651295237389157 2316 62-63 -2.282540401474705 2316 64-65 -2.07506257324928 2316 66-67 -2.277166062334601 2316 68-69 -2.45247621085678 2316 70-71 -2.7858908101328375 2316 72-73 -3.532767945735781 2316 74-75 -4.002683052568958 2316 76-77 -3.761361302235155 2316 78-79 -3.915564810664776 2316 80-81 -3.8540736217233302 2316 82-83 -3.3755436637463916 2316 84-85 -3.0819863968550685 2316 86-87 -3.3401260421392642 2316 88-89 -3.270504138592287 2316 90-91 -2.654960702867079 2316 92-93 -2.4611862024095856 2316 94-95 -1.623112831362505 2316 96-97 -1.8231466354167232 2316 98-99 -1.9652940995600865 2316 100-101 -2.084851148181066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10722.0 3 3172.0 4 3486.0 5 4062.0 6 4833.0 7 6034.0 8 7584.0 9 9392.0 10 11397.0 11 13667.0 12 16395.0 13 18012.0 14 20160.0 15 22093.0 16 25401.0 17 26655.0 18 24315.0 19 24350.0 20 25939.0 21 28430.0 22 31626.0 23 35087.0 24 39323.0 25 44310.0 26 49709.0 27 56135.0 28 62985.0 29 71864.0 30 81863.0 31 93339.0 32 108026.0 33 124598.0 34 144093.0 35 167576.0 36 174300.0 37 102951.0 38 13220.0 39 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.99312410444197 18.446754099665657 13.435360611367617 22.124761184524758 2 14.918370230446465 21.587464068772206 40.38825366171676 23.105912039064567 3 20.357893292345008 26.573993329818595 30.11568724156273 22.952426136273672 4 12.099125535536123 16.344621163213805 39.29274018428311 32.263513116966955 5 12.714452770834775 39.174291588912205 34.30223897665548 13.80901666359754 6 29.46172247400474 37.050179089523645 18.584482975056517 14.903615461415095 7 26.922097670063756 34.36990348446201 21.560533991632035 17.1474648538422 8 25.129265588426815 33.7764206895338 20.77893736470671 20.315376357332678 9 26.926600876575534 17.384632711096096 21.038751396045086 34.650015016283284 10-11 24.38506694631099 26.29920534796305 28.139767029535673 21.175960676190293 12-13 27.275924042406174 24.078127877825914 27.77447347150417 20.87147460826374 14-15 23.70535356826827 25.14002856151504 27.858505233784715 23.296112636431975 16-17 21.404048621942284 28.50183625669936 28.243276297243064 21.85083882411529 18-19 20.981132385381887 28.416897231085827 30.116322963629532 20.485647419902755 20-21 22.79028092397939 26.686267083006815 29.033065360572063 21.49038663244173 22-23 21.926751097049163 26.731025319137576 28.878084194451638 22.46413938936163 24-25 22.393453055004223 26.634167933213227 29.522980115072283 21.449398896710267 26-27 22.52931109426422 26.05600505054507 30.236675327846648 21.17800852734406 28-29 21.45184793854146 27.12428918385466 30.212947676594247 21.210915201009634 30-31 21.61425692213127 27.321657847723973 29.622107376033334 21.441977854111418 32-33 21.496369218486755 27.26614186170744 29.41666813424206 21.820820785563754 34-35 22.45454777564293 26.46698629690748 29.336820564411404 21.741645363038188 36-37 22.525876381408793 26.262010942700183 30.10067096369824 21.111441712192782 38-39 22.459198410365023 26.319878829064358 30.279265908820165 20.94165685175046 40-41 21.888393757054946 26.81334983924924 30.183848869345738 21.11440753435008 42-43 21.295169410963886 27.575228561033715 29.173013954252585 21.95658807374981 44-45 21.78908740901161 27.33623557134326 28.656592467386506 22.218084552258624 46-47 22.131978563037855 27.24477270045491 28.068648061274633 22.554600675232606 48-49 22.393236454621935 27.096597503650123 28.321260186442498 22.188905855285444 50-51 22.346606356360983 27.589586960714634 28.684179083796508 21.379627599127872 52-53 22.117540378645884 27.92625705082822 28.434530404342915 21.52167216618298 54-55 21.18203633145709 27.287084631196457 28.950160000869076 22.58071903647738 56-57 22.156002903695754 27.996652472060145 27.76061711460352 22.086727509640582 58-59 22.39906483757597 27.30259393728849 27.938329512132288 22.360011713003253 60-61 22.055968963450006 26.999051895724374 28.909550969481373 22.035428171344247 62-63 22.72276555104518 26.873075863258972 28.504735016053363 21.89942356964249 64-65 22.22428917155888 27.453041780022865 28.602174326376645 21.720494722041607 66-67 21.694719192059097 27.403988644786892 28.39319743621496 22.508094726939053 68-69 21.458545444942917 27.95249091159824 28.336477719845433 22.252485923613413 70-71 21.936501107927807 27.685342046117146 27.718736877195937 22.659419968759106 72-73 21.830573345621687 27.049591725320077 28.019324805842054 23.100510123216182 74-75 21.56670694885337 28.10610304488704 27.796910837787276 22.53027916847232 76-77 22.20463230682867 26.827866888706726 28.079895666225873 22.88760513823873 78-79 21.789572545838503 27.052945222793053 28.500124627768127 22.65735760360032 80-81 21.823331510095777 27.9260100251642 27.86168291579432 22.38897554894571 82-83 21.76354921023367 27.559562742710327 27.89581965778945 22.78106838926655 84-85 21.708637395501178 27.139744110866705 27.811442080100722 23.340176413531395 86-87 21.8590666098809 27.293094688309832 28.06119948094443 22.786639220864835 88-89 22.233124894332462 26.668549114271222 27.894644769222097 23.203681222174215 90-91 21.590840388271555 26.762110594117466 28.500522113364635 23.146526904246343 92-93 21.54535574743662 27.603256401880778 28.1767278027094 22.6746600479732 94-95 21.407925290212983 27.546474153362578 27.696032305030517 23.349568251393922 96-97 20.9962045657981 27.052336112657795 27.792569124829964 24.158890196714147 98-99 21.570516510913677 27.13823557747664 27.211520160162795 24.079727751446896 100-101 21.831633949301914 26.83412318098844 27.74684146604732 23.587401403662327 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2571.0 1 1757.0 2 884.0 3 750.0 4 641.0 5 641.0 6 893.0 7 1874.5 8 2585.0 9 2295.5 10 1939.5 11 1845.5 12 1887.5 13 2032.5 14 2224.0 15 2339.0 16 2485.0 17 2692.0 18 2924.0 19 3236.5 20 3663.5 21 4234.0 22 5001.0 23 5970.5 24 7185.0 25 8964.0 26 11238.0 27 13657.5 28 16268.5 29 19186.0 30 22131.0 31 25086.5 32 28223.5 33 31450.0 34 34836.0 35 37932.5 36 41276.0 37 44223.0 38 45504.5 39 46212.5 40 47600.5 41 49484.0 42 51580.5 43 54193.5 44 56835.5 45 59655.5 46 66121.0 47 87057.0 48 103467.5 49 90640.0 50 70347.0 51 60069.0 52 55151.5 53 51746.0 54 48141.5 55 43580.0 56 38792.5 57 34440.0 58 30594.0 59 26579.5 60 21983.5 61 17771.5 62 14039.0 63 10582.0 64 7742.5 65 5593.5 66 4016.5 67 2869.0 68 1987.5 69 1417.0 70 994.5 71 719.5 72 538.0 73 407.5 74 298.0 75 199.5 76 130.0 77 88.5 78 72.5 79 58.5 80 31.5 81 19.5 82 14.5 83 6.5 84 6.0 85 7.0 86 6.0 87 3.5 88 2.5 89 2.5 90 2.0 91 2.5 92 2.5 93 4.0 94 4.0 95 3.0 96 2.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.906223420831274 2 0.08640060357411473 3 0.11498602360405913 4 0.19119428479044778 5 0.11773912758235294 6 0.14234133334582968 7 0.1504249152395435 8 0.14843330810630967 9 0.1375380455539128 10-11 0.12541267271334214 12-13 0.1357807451422359 14-15 0.14190200824291047 16-17 0.13689370206963128 18-19 0.14471368890159353 20-21 0.17271334212764564 22-23 0.19183862827472933 24-25 0.2174952142852122 26-27 0.21002668753558532 28-29 0.20949949741208224 30-31 0.22393864912802755 32-33 0.21521072375003222 34-35 0.20059584199291924 36-37 0.18726964720436934 38-39 0.18305212621634478 40-41 0.18325714459770706 42-43 0.1771358814970325 44-45 0.19368379370699007 46-47 0.21972112814000297 48-49 0.22241565543790753 50-51 0.23269586284621746 52-53 0.2599925959075988 54-55 0.24628565269651892 56-57 0.22361647738588677 58-59 0.18012329219688325 60-61 0.18964200276013318 62-63 0.19421098383049312 64-65 0.1813534024850571 66-67 0.19195578163550778 68-69 0.17977183211454786 70-71 0.1937423703873793 72-73 0.2140391901422476 74-75 0.2447333706662043 76-77 0.244147603862312 78-79 0.2397250644929251 80-81 0.24306393527511122 82-83 0.23442387491769975 84-85 0.20481336298094382 86-87 0.21588435557450839 88-89 0.21720233088326604 90-91 0.20566272484658768 92-93 0.20528197642405765 94-95 0.24294678191433278 96-97 0.26898411634734565 98-99 0.2867914271856717 100-101 0.2830132313005663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1707164.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.33081328601322 #Duplication Level Percentage of deduplicated Percentage of total 1 85.17295914426627 63.309753231696874 2 9.72895215048764 14.463218515329068 3 2.6276644652977135 5.859493102450082 4 1.0324252664967992 3.069640388629441 5 0.497928523701271 1.850571606250969 6 0.2637480993859769 1.1762766437999939 7 0.16071753622097684 0.8362385623640648 8 0.11182792577814245 0.66498085369418 9 0.0840447572800097 0.562240364494383 >10 0.29869309350764933 3.6926716576662786 >50 0.011776234021577823 0.5992896926887658 >100 0.006591026337799603 1.0144779655156697 >500 0.0012468293685686978 0.6490096807935967 >1k 0.0014249478497927977 2.2521377346265763 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4557 0.2669339325337226 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3771 0.22089266174778757 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3756 0.2200140115419491 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3330 0.19506034569613698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2358 0.1381238123578051 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1961 0.11486887024328067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1724 0.10098619699103308 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.8576680389230325E-5 0.0 0.0 0.0 0.0 3 5.8576680389230325E-5 0.0 0.0 0.0 0.0 4 5.8576680389230325E-5 0.0 0.0 0.0 0.0 5 5.8576680389230325E-5 0.0 0.0 0.0 0.0 6 5.8576680389230325E-5 0.0 0.0 0.0 0.0 7 5.8576680389230325E-5 0.0 0.0 0.0 0.0 8 5.8576680389230325E-5 0.0 0.0 0.0 0.0 9 5.8576680389230325E-5 0.0 0.0 0.0 0.0 10-11 5.8576680389230325E-5 0.0 0.0 0.0 0.0 12-13 5.8576680389230325E-5 0.0 0.0 0.0 0.0 14-15 5.8576680389230325E-5 0.0 0.0 2.9288340194615162E-5 0.0 16-17 5.8576680389230325E-5 0.0 0.0 2.0501838136230614E-4 0.0 18-19 5.8576680389230325E-5 0.0 0.0 2.343067215569213E-4 0.0 20-21 5.8576680389230325E-5 0.0 0.0 4.1003676272461227E-4 0.0 22-23 5.8576680389230325E-5 0.0 0.0 6.736318244761488E-4 0.0 24-25 1.1715336077846065E-4 0.0 0.0 0.0014058403293415278 0.0 26-27 1.1715336077846065E-4 0.0 0.0 0.0020501838136230614 0.0 28-29 2.343067215569213E-4 0.0 0.0 0.004773999451722272 0.0 30-31 2.928834019461516E-4 0.0 5.8576680389230325E-5 0.011568894376872989 0.0 32-33 2.928834019461516E-4 0.0 5.8576680389230325E-5 0.028116806586830558 0.0 34-35 2.928834019461516E-4 0.0 5.8576680389230325E-5 0.055940729771714964 0.0 36-37 2.928834019461516E-4 0.0 5.8576680389230325E-5 0.09682725268339773 0.0 38-39 2.928834019461516E-4 0.0 5.8576680389230325E-5 0.15194790892966348 0.0 40-41 2.928834019461516E-4 0.0 5.8576680389230325E-5 0.21474211030691837 0.0 42-43 2.928834019461516E-4 0.0 5.8576680389230325E-5 0.2806115874046079 0.0 44-45 2.928834019461516E-4 0.0 5.8576680389230325E-5 0.3572005970135265 0.0 46-47 3.5146008233538195E-4 0.0 5.8576680389230325E-5 0.45147976409999274 0.0 48-49 3.5146008233538195E-4 0.0 5.8576680389230325E-5 0.554047531461535 0.0 50-51 3.5146008233538195E-4 0.0 5.8576680389230325E-5 0.664786745737375 0.0 52-53 3.5146008233538195E-4 0.0 5.8576680389230325E-5 0.7693461202321511 0.0 54-55 4.1003676272461227E-4 0.0 5.8576680389230325E-5 0.8851522173616595 0.0 56-57 4.1003676272461227E-4 0.0 5.8576680389230325E-5 1.022397379513626 0.0 58-59 4.1003676272461227E-4 0.0 5.8576680389230325E-5 1.1792364412557903 0.0 60-61 4.1003676272461227E-4 0.0 5.8576680389230325E-5 1.3690248857168967 0.0 62-63 4.1003676272461227E-4 0.0 5.8576680389230325E-5 1.5557087661173736 0.0 64-65 4.1003676272461227E-4 0.0 5.8576680389230325E-5 1.7307358871203937 0.0 66-67 4.1003676272461227E-4 0.0 5.8576680389230325E-5 1.9303066372065016 0.0 68-69 4.1003676272461227E-4 0.0 5.8576680389230325E-5 2.1600443776930627 0.0 70-71 4.1003676272461227E-4 2.9288340194615162E-5 5.8576680389230325E-5 2.413388520376484 0.0 72-73 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 2.687820268000028 0.0 74-75 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 2.963101377489216 0.0 76-77 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 3.2459681670888094 0.0 78-79 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 3.577131429669323 0.0 80-81 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 3.944612234091159 0.0 82-83 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 4.312971688718834 0.0 84-85 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 4.71211318889105 0.0 86-87 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 5.134597496198373 0.0 88-89 4.1003676272461227E-4 5.8576680389230325E-5 5.8576680389230325E-5 5.560010637525158 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7685 0.0 31.046974 1 TACATGG 9055 0.0 26.130234 2 ACATGGG 9095 0.0 24.821728 3 GTATAAT 755 0.0 23.900763 1 CATGGGA 6830 0.0 22.194883 4 GAGTACT 6050 0.0 21.87739 12-13 GTATAGC 545 2.6466296E-9 20.234032 1 ATGGGAG 2600 0.0 19.962652 5 GTACTTT 6715 0.0 19.78429 14-15 AGTACTT 6585 0.0 19.670012 12-13 TACCTGG 1155 0.0 19.218077 2 CATGGGG 2880 0.0 19.02103 4 GTTCTAA 1060 0.0 18.915173 1 GGATCGT 155 0.0010065803 18.265486 6 GGTTAGT 335 0.0011284966 17.9553 1 TAGCCCT 1130 0.0 17.552515 4 GTATATG 860 2.910383E-11 17.485538 1 GTATAAG 1385 0.0 17.371914 1 ATGGGAT 3125 0.0 17.212889 5 GCCTTAG 700 1.2862074E-8 17.185785 1 >>END_MODULE