##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939680_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1707164 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.486236823175748 31.0 31.0 34.0 30.0 34.0 2 31.674666874418627 31.0 31.0 34.0 30.0 34.0 3 31.73207143543327 31.0 31.0 34.0 30.0 34.0 4 35.50881754769899 37.0 35.0 37.0 33.0 37.0 5 35.28201332736632 37.0 35.0 37.0 33.0 37.0 6 35.30156446597984 37.0 35.0 37.0 32.0 37.0 7 35.21491725458128 37.0 35.0 37.0 32.0 37.0 8 35.187201698255116 37.0 35.0 37.0 32.0 37.0 9 36.76526332560902 39.0 35.0 39.0 32.0 39.0 10-11 36.65062934785411 39.0 35.0 39.0 32.0 39.0 12-13 36.59003382217526 39.0 35.0 39.0 32.0 39.0 14-15 37.5937127305871 39.0 36.0 41.0 32.0 41.0 16-17 37.56575935293856 39.0 36.0 41.0 32.0 41.0 18-19 37.590709211300144 39.0 36.0 41.0 32.0 41.0 20-21 37.538543162812715 39.0 36.0 41.0 32.0 41.0 22-23 37.404899294971074 39.0 36.0 41.0 32.0 41.0 24-25 37.26260687315337 39.0 36.0 40.5 31.0 41.0 26-27 36.938822222118084 39.0 36.0 40.0 30.5 41.0 28-29 36.7423700944959 39.0 35.5 40.0 30.0 41.0 30-31 36.52147128219667 38.0 35.0 40.0 30.0 41.0 32-33 36.356836835828304 38.0 35.0 40.0 30.0 41.0 34-35 36.32303662682671 38.0 35.0 40.0 30.0 41.0 36-37 36.20814198284406 38.0 35.0 40.0 30.0 41.0 38-39 36.223223134977076 38.0 35.0 40.0 30.0 41.0 40-41 36.11340796783438 38.0 35.0 40.0 30.0 41.0 42-43 35.94935196618486 38.0 35.0 40.0 29.0 41.0 44-45 35.72673920021744 38.0 35.0 40.0 28.5 41.0 46-47 35.40817431717164 38.0 34.0 40.0 27.0 41.0 48-49 35.08574747358777 38.0 34.0 40.0 26.0 41.0 50-51 34.84152606310818 38.0 34.0 40.0 26.0 41.0 52-53 34.58312616714036 38.0 33.0 40.0 24.5 41.0 54-55 34.31324465604945 38.0 33.0 40.0 24.0 41.0 56-57 33.50768789641768 37.5 32.0 40.0 21.0 41.0 58-59 30.30766317705856 35.5 25.5 39.0 8.0 40.5 60-61 30.01839219899201 34.5 25.5 39.0 10.0 40.0 62-63 31.937551693920447 35.5 30.5 39.0 17.0 40.0 64-65 31.99062128770288 36.0 31.0 39.0 12.0 40.0 66-67 26.97094567364354 31.5 18.5 37.0 2.0 39.5 68-69 19.449646606887214 19.0 8.0 31.5 2.0 37.0 70-71 17.52210888936271 15.0 7.5 29.0 2.0 35.0 72-73 19.20667580853392 19.0 7.5 30.5 2.0 35.0 74-75 20.651491889472833 22.5 9.0 32.0 2.0 36.0 76-77 22.479948616535964 27.0 11.0 33.0 2.0 35.5 78-79 24.678919248531482 30.5 13.0 34.5 2.0 36.0 80-81 26.321144014283337 31.5 22.5 35.0 2.0 36.5 82-83 27.517400495793023 33.0 26.0 35.0 2.0 36.0 84-85 27.588976805977634 33.5 27.0 35.0 2.0 36.0 86-87 27.314885681750553 33.0 26.0 35.0 2.0 36.0 88-89 26.84590789168469 33.0 25.5 35.0 2.0 35.0 90-91 24.969818072546047 32.0 15.0 34.5 2.0 35.0 92-93 23.324286360302818 31.0 2.0 34.0 2.0 35.0 94-95 19.51161399842077 26.5 2.0 34.0 2.0 35.0 96-97 16.820212645065148 15.5 2.0 33.0 2.0 35.0 98-99 17.08838635303931 19.0 2.0 33.0 2.0 35.0 100-101 16.319132783962175 2.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1613499850623228 1101 2 0.03434130189845064 1101 3 0.18139897750037548 1101 4 0.11585514865253543 1101 5 0.2661641638830261 1101 6 0.22166817073004808 1101 7 0.227040542902607 1101 8 0.17011881682072527 1101 9 0.22955186604020383 1101 10-11 0.10450417124962996 1101 12-13 0.1503871304891149 1101 14-15 0.29447664649482874 1101 16-17 0.3197796094144252 1101 18-19 0.23861366249956006 1101 20-21 0.39136620141540135 1101 22-23 0.34561627985115706 1101 24-25 0.2921845503150209 1101 26-27 0.1888069043109084 1101 28-29 0.2965014278793916 1101 30-31 0.12933805122430897 1101 32-33 0.1390942317264603 1101 34-35 0.015787257969066104 1101 36-37 0.09077224510704696 1101 38-39 -0.1324256426586956 1101 40-41 -0.0269221200672618 1101 42-43 -0.024920174586625876 1101 44-45 0.08514633146695161 1101 46-47 -0.1876189269346895 1101 48-49 -0.4134966395469917 1101 50-51 -0.3116588860171774 1101 52-53 -0.3007595764450741 1101 54-55 -0.34501495928127923 1101 56-57 -3.670531446100675 1101 58-59 -10.008627394515997 1101 60-61 -11.545074360826309 1101 62-63 -4.220078589996394 1101 64-65 -1.4079317675887317 1101 66-67 -2.4394377151404285 1101 68-69 -4.922975678666024 1101 70-71 -4.463754779339867 1101 72-73 -3.56863392006273 1101 74-75 -6.433180044141292 1101 76-77 -4.885379539230758 1101 78-79 1.0087101036775117 1101 80-81 1.6684085773482416 1101 82-83 0.13554945993264056 1101 84-85 -0.12049766538389406 1101 86-87 -0.11541186691977501 1101 88-89 -0.09507451826783964 1101 90-91 1.4071191442378463 1101 92-93 1.2709324042658245 1101 94-95 -8.254643107974122 1101 96-97 -9.42979466063806 1101 98-99 -8.420047385298746 1101 100-101 -9.421899236360815 1102 1 0.2828293944134046 1102 2 0.36493059169869113 1102 3 0.24841127322396872 1102 4 0.2716032747030539 1102 5 0.20107785984300364 1102 6 0.3385317375737458 1102 7 0.37131271761301576 1102 8 0.3867520216399143 1102 9 0.3836535659708673 1102 10-11 0.4234962212651965 1102 12-13 0.4266047890544016 1102 14-15 0.3920291144996284 1102 16-17 0.4199896706779995 1102 18-19 0.40419088366946454 1102 20-21 0.35935766477339115 1102 22-23 0.3658017737531907 1102 24-25 0.3308691126185721 1102 26-27 0.12625088367294524 1102 28-29 0.23944768763622903 1102 30-31 0.30722131840812494 1102 32-33 0.1685040989285227 1102 34-35 0.2276140855966844 1102 36-37 -0.045353819177549326 1102 38-39 0.212493747836767 1102 40-41 0.001797005141987995 1102 42-43 0.20632398679410358 1102 44-45 0.041025724302159006 1102 46-47 0.07525442212313749 1102 48-49 0.09393077232964231 1102 50-51 0.12940052123500578 1102 52-53 0.10006260437276637 1102 54-55 -0.04886169541783403 1102 56-57 -3.4258892126040763 1102 58-59 -9.912012116300954 1102 60-61 -7.8652147801574515 1102 62-63 -2.3614002727574324 1102 64-65 -0.6069600837094278 1102 66-67 -1.9851178680498904 1102 68-69 -4.938257592580474 1102 70-71 -4.556398506146678 1102 72-73 -3.460396026111841 1102 74-75 -4.095340544021724 1102 76-77 -3.78823480684812 1102 78-79 2.01040900210317 1102 80-81 2.4114504055177868 1102 82-83 0.889660794122733 1102 84-85 0.6078153585864889 1102 86-87 0.5975078470115385 1102 88-89 0.4229944492664295 1102 90-91 1.8918922832344052 1102 92-93 1.351647283519302 1102 94-95 -8.741728469233044 1102 96-97 -9.713901229310544 1102 98-99 -8.586287391905095 1102 100-101 -9.458222975004002 1103 1 0.24187935768813418 1103 2 0.25838546948221364 1103 3 0.23071980962096106 1103 4 0.14604498039479807 1103 5 0.23748244593073764 1103 6 0.23959675605961195 1103 7 0.2337616095931523 1103 8 0.3129303611533558 1103 9 0.3334647525194754 1103 10-11 0.2891490813512263 1103 12-13 0.3829796682249693 1103 14-15 0.3772878521189966 1103 16-17 0.4596283158064338 1103 18-19 0.3991142563875343 1103 20-21 0.3731865986687666 1103 22-23 0.3527090647293676 1103 24-25 0.527553492815521 1103 26-27 0.5280468252942399 1103 28-29 0.4635906190033907 1103 30-31 0.3970516427912685 1103 32-33 0.38975787068390844 1103 34-35 0.16422581194800046 1103 36-37 0.22638738273462877 1103 38-39 0.3498372284064786 1103 40-41 0.3404544278734747 1103 42-43 0.4009315193288501 1103 44-45 0.4414288855124795 1103 46-47 0.2597673057718026 1103 48-49 0.17182416616581264 1103 50-51 0.0763682577347069 1103 52-53 0.13133052459912165 1103 54-55 0.23773998629286552 1103 56-57 0.6344562943751626 1103 58-59 -8.559944310248202 1103 60-61 -7.872844838192833 1103 62-63 -2.2988377803339013 1103 64-65 -0.2555453942321151 1103 66-67 -1.7865857611657425 1103 68-69 -3.411917277863685 1103 70-71 -4.057425753344855 1103 72-73 -3.1157154012635484 1103 74-75 -3.76251015917013 1103 76-77 -0.6058645410477297 1103 78-79 3.1744260903151726 1103 80-81 2.6783612959429384 1103 82-83 1.0804582411505095 1103 84-85 0.6372629205473075 1103 86-87 0.5385690195893496 1103 88-89 0.47478281083795437 1103 90-91 1.8299973796930438 1103 92-93 1.3915101777571195 1103 94-95 -7.430390749501157 1103 96-97 -8.826597941632322 1103 98-99 -8.076484195216128 1103 100-101 -9.508024761717735 1104 1 0.21955226611983036 1104 2 0.18891550712164573 1104 3 0.2088280135786924 1104 4 0.1203549568961435 1104 5 0.19273777527999414 1104 6 0.118261430127518 1104 7 0.20759104681139462 1104 8 0.21899926318010898 1104 9 0.34823726072658445 1104 10-11 0.3305177527753358 1104 12-13 0.22856694062217997 1104 14-15 0.36023784062539477 1104 16-17 0.24338999417557972 1104 18-19 0.1720786947944859 1104 20-21 0.12449698152087052 1104 22-23 0.20615624559368229 1104 24-25 0.21524468984168976 1104 26-27 0.19533224483313205 1104 28-29 0.1785690003559992 1104 30-31 0.30029871136113684 1104 32-33 0.25271467104541046 1104 34-35 0.3244919857481108 1104 36-37 0.2999739649537716 1104 38-39 0.17391811974783167 1104 40-41 0.2593741025006082 1104 42-43 0.15928995217502973 1104 44-45 0.21771775650369563 1104 46-47 0.28342192223682616 1104 48-49 0.23406777316673555 1104 50-51 0.1552178691723256 1104 52-53 0.44582423571688423 1104 54-55 0.36649570990915237 1104 56-57 0.8966167484089098 1104 58-59 -8.490184197706466 1104 60-61 -7.779544061630112 1104 62-63 -2.023193141100876 1104 64-65 -0.13814458906184868 1104 66-67 -1.5236539151249104 1104 68-69 -3.2163432694747627 1104 70-71 -3.8149343904061794 1104 72-73 -2.8805672968083584 1104 74-75 -3.5876254745472203 1104 76-77 -0.12164393361235071 1104 78-79 3.747781395440761 1104 80-81 2.892316774961916 1104 82-83 1.3898977187733976 1104 84-85 0.9293767088773386 1104 86-87 0.995444693679179 1104 88-89 0.8524309054575454 1104 90-91 2.3906168482222157 1104 92-93 1.8898978420046042 1104 94-95 -6.153034434962063 1104 96-97 -7.975581137370305 1104 98-99 -6.061286086573784 1104 100-101 -5.413690544679218 1105 1 0.16039596798565015 1105 2 0.25461719710585484 1105 3 0.15566675773122896 1105 4 0.12608167522564173 1105 5 0.23208662725308926 1105 6 0.1502673401054011 1105 7 0.1408868528508549 1105 8 0.22473454504014967 1105 9 0.2670110316601324 1105 10-11 0.2489561187623579 1105 12-13 0.23852233033704096 1105 14-15 0.39619667630494604 1105 16-17 0.41432206025863394 1105 18-19 0.3178688060861319 1105 20-21 0.3137584283921626 1105 22-23 0.36341692060541675 1105 24-25 0.31060302792302963 1105 26-27 0.311980947115714 1105 28-29 0.3777013434407124 1105 30-31 0.3249334196929894 1105 32-33 0.4336032686473885 1105 34-35 0.48326612205895714 1105 36-37 0.32204703584724825 1105 38-39 0.4528442986890866 1105 40-41 0.33244870683694927 1105 42-43 0.3070585244746411 1105 44-45 0.37892935905997405 1105 46-47 0.649126067099786 1105 48-49 0.4310461028672279 1105 50-51 0.2655952125824612 1105 52-53 0.3366494974469276 1105 54-55 0.2202203318863667 1105 56-57 0.7402198481786044 1105 58-59 -8.241139316813754 1105 60-61 -7.617691621537716 1105 62-63 -2.1481441862512334 1105 64-65 -0.324226682491112 1105 66-67 -1.7802912222028375 1105 68-69 -3.2222623817906033 1105 70-71 -3.929889797248098 1105 72-73 -2.8514241750931895 1105 74-75 -3.2647572512971195 1105 76-77 0.037130773600534184 1105 78-79 3.716017547926409 1105 80-81 3.086097196904195 1105 82-83 1.4478174430962198 1105 84-85 1.0573084335609728 1105 86-87 0.8503091215725398 1105 88-89 0.9732814467297679 1105 90-91 2.2828630851214413 1105 92-93 1.838220931452394 1105 94-95 -6.472878304673081 1105 96-97 -7.772401669747872 1105 98-99 -5.7457325456680834 1105 100-101 -5.090398712117935 1106 1 0.2550438687092935 1106 2 0.30786229007338406 1106 3 0.29617943187811235 1106 4 0.2851890442475664 1106 5 0.3163038507735436 1106 6 0.32165938539102257 1106 7 0.32773107055006534 1106 8 0.35828847928799945 1106 9 0.3605939829554927 1106 10-11 0.22698848941946181 1106 12-13 0.33699388114362705 1106 14-15 0.362013940258997 1106 16-17 0.48507400612519547 1106 18-19 0.3527852928827784 1106 20-21 0.4000478734411317 1106 22-23 0.323911221252942 1106 24-25 0.3333772479412218 1106 26-27 0.4169524949492427 1106 28-29 0.5468299995589732 1106 30-31 0.5254768947222743 1106 32-33 0.45761636199546984 1106 34-35 0.5041194492083321 1106 36-37 0.4828680755851451 1106 38-39 0.669021302678928 1106 40-41 0.6440546339256699 1106 42-43 0.5680612180653242 1106 44-45 0.5758769202603773 1106 46-47 0.40542835575995184 1106 48-49 0.3872782273551252 1106 50-51 0.42572888127676123 1106 52-53 0.611732297116319 1106 54-55 0.6663919249103856 1106 56-57 1.0719078911333568 1106 58-59 -7.862391033754836 1106 60-61 -7.335998227078306 1106 62-63 -1.6763284801796523 1106 64-65 0.14633439304240525 1106 66-67 1.0996412299160596 1106 68-69 -1.8895096867133923 1106 70-71 -1.8250588750649221 1106 72-73 -0.5536391034127526 1106 74-75 -0.5670949669181624 1106 76-77 2.465570155917977 1106 78-79 4.820402511387524 1106 80-81 3.853412882164367 1106 82-83 2.2805172177384136 1106 84-85 1.971248508600958 1106 86-87 1.9281054314127672 1106 88-89 1.780198266508929 1106 90-91 3.245101678983861 1106 92-93 2.774881414158113 1106 94-95 -5.381227543867503 1106 96-97 -7.443487490044344 1106 98-99 -5.408336528376777 1106 100-101 -4.934375296311968 1107 1 0.09172202871658186 1107 2 0.04024058852986556 1107 3 0.09502245518519459 1107 4 0.10533825191253499 1107 5 0.04230261031581506 1107 6 0.06926974056758439 1107 7 -9.517220671497739E-4 1107 8 -0.007075412809818715 1107 9 0.059009900791338055 1107 10-11 -0.012023171471135186 1107 12-13 0.11179010215673912 1107 14-15 0.20046930582659428 1107 16-17 0.14566301778608448 1107 18-19 0.09897823403157702 1107 20-21 0.13966594767458673 1107 22-23 0.1782168147766967 1107 24-25 0.10308465276842327 1107 26-27 0.1947097943963314 1107 28-29 0.13717456461980504 1107 30-31 0.06599812212874667 1107 32-33 0.1504319952902904 1107 34-35 -0.05599513146110269 1107 36-37 0.11693995158639581 1107 38-39 0.25773387216072763 1107 40-41 0.039649098309546105 1107 42-43 -0.10289531594291645 1107 44-45 -0.2017050396040574 1107 46-47 -0.1205886285168063 1107 48-49 -0.244081690574788 1107 50-51 -0.22377655233965044 1107 52-53 -0.12942312165574066 1107 54-55 -0.12528627918900526 1107 56-57 0.5530377952771275 1107 58-59 -8.129304763152803 1107 60-61 -7.578354420695536 1107 62-63 -2.25703186674685 1107 64-65 -0.4321585095993008 1107 66-67 0.6578607321456502 1107 68-69 -2.6222883414340394 1107 70-71 -2.3307876145893722 1107 72-73 -0.7572794941275482 1107 74-75 -0.6217140552699121 1107 76-77 2.290800390164602 1107 78-79 4.191742793635754 1107 80-81 3.124529029573072 1107 82-83 1.3133262960077694 1107 84-85 1.1284678550384655 1107 86-87 1.0185977050071848 1107 88-89 0.9947773192342595 1107 90-91 2.1795259978344106 1107 92-93 1.7626855394149494 1107 94-95 -6.811745890590137 1107 96-97 -8.226286280157133 1107 98-99 -6.039750091694501 1107 100-101 -5.265323394031819 1108 1 0.001323863637029632 1108 2 -0.029757991909164616 1108 3 -0.09818327635753832 1108 4 -0.0858380009516253 1108 5 -0.09426002585245641 1108 6 -0.07738455163031688 1108 7 -0.042860703533605715 1108 8 0.005963936897721567 1108 9 -0.002002191023194655 1108 10-11 -0.10867263019457596 1108 12-13 -0.0885516085216338 1108 14-15 -0.24487077482420716 1108 16-17 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0.1185162681638019 2312 86-87 0.08648139597195836 2312 88-89 0.1595486464218645 2312 90-91 1.9332238953938514 2312 92-93 3.4169475730920524 2312 94-95 5.5012002638087765 2312 96-97 0.5713623623348774 2312 98-99 -0.8599349407515042 2312 100-101 -0.38780618644014275 2313 1 -0.201037216372022 2313 2 -0.2808316220331335 2313 3 -0.25123367221936377 2313 4 -0.2353302886476598 2313 5 -0.33948299302992524 2313 6 -0.1398992053294208 2313 7 -0.20305947347068098 2313 8 -0.1481818686508376 2313 9 -0.2813404314333212 2313 10-11 -0.2531019734925195 2313 12-13 -0.24994079966082694 2313 14-15 -0.38952683021504697 2313 16-17 -0.453216434289466 2313 18-19 -0.47162045222257376 2313 20-21 -0.34355992720892203 2313 22-23 -0.2623710372412731 2313 24-25 -0.3855924339585002 2313 26-27 -0.4965769795014907 2313 28-29 -0.4064294478357766 2313 30-31 -0.522571758694653 2313 32-33 -0.4780673493520595 2313 34-35 -0.39114427267741547 2313 36-37 -0.5158741465581471 2313 38-39 -0.5095630237927153 2313 40-41 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0.7110141585224419 2314 64-65 0.07608582580263246 2314 66-67 -1.851979333979596 2314 68-69 -4.926331624604902 2314 70-71 -3.557829100308158 2314 72-73 -3.0930963508168237 2314 74-75 -4.365398372828526 2314 76-77 0.43419967380778957 2314 78-79 3.423184428923541 2314 80-81 2.6015754353249214 2314 82-83 1.1818515324973582 2314 84-85 0.8541038513123596 2314 86-87 0.7191759728931437 2314 88-89 0.9111531572798945 2314 90-91 2.4188446060554583 2314 92-93 3.7940146699467086 2314 94-95 6.24590705683767 2314 96-97 3.3934132042234033 2314 98-99 5.710322068790415 2314 100-101 6.328030953309396 2315 1 -0.5107774965100162 2315 2 -0.5069203888580738 2315 3 -0.45235503722225445 2315 4 -0.22865451440628703 2315 5 -0.36333093923692417 2315 6 -0.47280343967413785 2315 7 -0.46463698507020723 2315 8 -0.5463788521876012 2315 9 -0.5075563881259413 2315 10-11 -0.46674599707776565 2315 12-13 -0.6127047802294214 2315 14-15 -0.842312288317224 2315 16-17 -0.8271426346014934 2315 18-19 -0.9120646435875912 2315 20-21 -0.8705888519710427 2315 22-23 -0.641546052346996 2315 24-25 -0.5906569928252381 2315 26-27 -0.5005732974626085 2315 28-29 -0.6234761853386814 2315 30-31 -0.5988464136651857 2315 32-33 -0.7724034890749039 2315 34-35 -0.6243045497727095 2315 36-37 -0.5227584136334613 2315 38-39 -0.7345697528450401 2315 40-41 -0.8552027372209992 2315 42-43 -0.7227411193283189 2315 44-45 -0.6102542024279387 2315 46-47 -0.584094379773461 2315 48-49 -0.543941231043064 2315 50-51 -0.3473578514358806 2315 52-53 -0.3659712850854362 2315 54-55 -0.3853064444437848 2315 56-57 0.32003368913705543 2315 58-59 3.5715482387453683 2315 60-61 3.464806533450684 2315 62-63 0.8297478423707254 2315 64-65 0.1278422977448379 2315 66-67 -1.7223154829455076 2315 68-69 -4.888936832663806 2315 70-71 -3.6248936048076654 2315 72-73 -3.1914762065855093 2315 74-75 -0.4846594362981165 2315 76-77 2.093908879174176 2315 78-79 3.7827766559120164 2315 80-81 2.6802080992679755 2315 82-83 0.7381746361051569 2315 84-85 0.5935020555429951 2315 86-87 0.5258003462457701 2315 88-89 0.7080358978009222 2315 90-91 2.1351110608668833 2315 92-93 3.869737843388581 2315 94-95 6.436050809146039 2315 96-97 3.99203287827223 2315 98-99 6.344151068587468 2315 100-101 6.5750934157294605 2316 1 -0.4832690690264094 2316 2 -0.40348420419275755 2316 3 -0.4883680167480726 2316 4 -0.3941421340792246 2316 5 -0.4745046615022943 2316 6 -0.48347992038900856 2316 7 -0.330948603120369 2316 8 -0.5299532662673059 2316 9 -0.6535867297359559 2316 10-11 -0.5346341150598732 2316 12-13 -0.4670092359194129 2316 14-15 -0.49577005881743474 2316 16-17 -0.6936010496740792 2316 18-19 -0.5824230885533836 2316 20-21 -0.5716657415823434 2316 22-23 -0.7701355731748265 2316 24-25 -0.6751148247132832 2316 26-27 -0.7466221221522744 2316 28-29 -0.5487416544085448 2316 30-31 -0.4103019862136108 2316 32-33 -0.5083354966976756 2316 34-35 -0.3462047638294621 2316 36-37 -0.20578927837468086 2316 38-39 -0.4812134391051117 2316 40-41 -0.48525922738426175 2316 42-43 -0.5272958973044979 2316 44-45 -0.6144332467526326 2316 46-47 -0.5918182385600659 2316 48-49 -0.5708434535499833 2316 50-51 -0.39989934161248897 2316 52-53 -0.24435078608232175 2316 54-55 -0.05632030475174332 2316 56-57 0.6083640598395803 2316 58-59 3.677409243317431 2316 60-61 3.660319982726975 2316 62-63 0.9603980264008136 2316 64-65 0.2145047540060041 2316 66-67 -1.8685368274994723 2316 68-69 -3.0040540567588003 2316 70-71 -3.018531321766991 2316 72-73 -0.42867950978541813 2316 74-75 5.782905995138329 2316 76-77 5.938018782101306 2316 78-79 4.996701287651668 2316 80-81 3.4323525034989295 2316 82-83 1.6787181720984528 2316 84-85 1.375851948914974 2316 86-87 1.244250663460793 2316 88-89 1.1073147907912322 2316 90-91 2.7790593869804745 2316 92-93 4.222088564161961 2316 94-95 6.535308283116258 2316 96-97 3.7235149343380165 2316 98-99 6.197581081105252 2316 100-101 6.331334124145203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 16.0 4 29.0 5 68.0 6 162.0 7 404.0 8 904.0 9 1583.0 10 2633.0 11 4239.0 12 6055.0 13 8419.0 14 10376.0 15 13791.0 16 18331.0 17 24245.0 18 25823.0 19 25004.0 20 25832.0 21 26804.0 22 27202.0 23 27184.0 24 28548.0 25 30241.0 26 37783.0 27 53353.0 28 75664.0 29 101049.0 30 121671.0 31 134394.0 32 139891.0 33 138988.0 34 141803.0 35 149926.0 36 148258.0 37 110504.0 38 43042.0 39 2944.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.933448603776505 19.41652687537609 14.220392321840079 22.42963219900733 2 15.105252921023402 22.23451845880572 39.22953174577137 23.43069687439951 3 20.19939548724197 26.403969165163193 30.652838632582768 22.743796715012067 4 12.396055680649312 17.02970540615899 38.663596467591866 31.910642445599834 5 12.510484505790728 39.51780944604883 34.37100878090182 13.600697267258624 6 29.74963155268035 37.39705148421593 18.042086173326055 14.811230789777666 7 26.66855674088723 34.41637710260994 21.387576120396165 17.527490036106666 8 25.083545039343054 34.31265790085657 20.524519334123337 20.079277725677045 9 26.20175917486545 17.22177834115527 21.114140176339237 35.46232230764004 10-11 24.304489785398474 26.50632862454925 28.008644746491846 21.180536843560432 12-13 26.91583819715036 24.147562858635727 27.86501472617745 21.071584218036463 14-15 23.27795746306054 25.290725515573747 27.776357282877356 23.654959738488355 16-17 21.187683198567917 28.688368545728473 28.25249946695221 21.8714487887514 18-19 20.821312861090107 28.4520111507026 30.082529023399722 20.64414696480757 20-21 22.45826997289071 26.83605675846023 29.028171868666398 21.677501399982663 22-23 21.720233088326605 26.822203373548177 28.89725298799647 22.56031055012875 24-25 22.17217815298138 26.80097143680565 29.448874799228193 21.577975610984783 26-27 22.368403710835963 26.193142129117764 30.106331412504666 21.332122747541607 28-29 21.28845887995651 27.185780026102012 30.21407634253152 21.311684751409953 30-31 21.524265833114146 27.344820455622525 29.617192255038017 21.513721456225312 32-33 21.395368999701827 27.371634639886917 29.303112191069914 21.929884169341342 34-35 22.28727278798055 26.569333915595937 29.217076461799852 21.926316834623655 36-37 22.37861457818183 26.36006286312145 29.962702504469807 21.29862005422692 38-39 22.44471542808902 26.384928358698968 30.122206458269822 21.048149754942195 40-41 21.835528662894646 26.898647624614618 30.02764083738047 21.238182875110258 42-43 21.179131089418675 27.633048598085907 29.16615119599944 22.02166911649598 44-45 21.669258789642257 27.412689920037526 28.633777732173353 22.284273558146865 46-47 22.020438082880972 27.332996988433038 28.060750807418312 22.585814121267678 48-49 22.204380597614303 27.086986420628122 28.438797267358794 22.269835714398777 50-51 22.16248648398317 27.593397178468287 28.728200339245063 21.51591599830348 52-53 22.071968439183266 27.8612247840686 28.432430784867524 21.63437599188061 54-55 21.161177265248053 27.25096227529205 28.983043148907615 22.60481731055228 56-57 21.953529241769754 27.899630290286897 28.01085974654339 22.13598072139996 58-59 21.204578570381255 26.94752829006935 29.637507718423645 22.21038542112575 60-61 21.230350282147157 26.977682440516336 29.78249056546105 22.009476711875458 62-63 22.61423336595184 26.90906875573521 28.52885449284565 21.947843385467298 64-65 22.03509121343079 27.559006456771556 28.642437786392012 21.763464543405643 66-67 21.509128432990586 27.09884069241583 29.055570181686157 22.336460692907426 68-69 20.992176359178366 27.197455591944426 30.006311226243664 21.804056822633544 70-71 21.987538270151667 27.07486362768206 28.682487344726855 22.25511075743942 72-73 21.25423973486073 26.750266344230084 29.207493190774052 22.788000730135135 74-75 21.21196928321064 27.56839261954524 28.868590347380955 22.351047749863163 76-77 20.513198786857007 27.095464345262453 29.366729090204718 23.024607777675822 78-79 21.442409926692566 27.048528991632526 28.85697539827231 22.652085683402596 80-81 21.409490266975105 27.9909108622814 28.115937782478944 22.483661088264554 82-83 21.556526173139552 27.695609170901037 27.891079954110936 22.85678470184848 84-85 21.545092987748177 27.24139617669034 27.81621941107711 23.397291424484376 86-87 21.701449653146433 27.510576947014954 27.949939445239313 22.838033954599307 88-89 22.134435278167558 26.8563614439976 27.787877376712895 23.221325901121947 90-91 21.351297982326848 26.98417455940535 28.43998227513041 23.224545183137398 92-93 21.09062892698389 27.828688551493897 28.258898428268658 22.82178409325356 94-95 20.147911802930142 27.810228151442523 28.516047188655563 23.52581285697177 96-97 19.669513087022086 27.518867609888343 28.450753632372244 24.360865670717327 98-99 20.560128095307437 27.373828067657996 27.9237806980982 24.14226313893636 100-101 20.10910901180345 27.31763860290977 28.64942249401285 23.92382989127393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 746.0 1 544.5 2 367.0 3 416.5 4 446.0 5 490.0 6 678.5 7 1019.0 8 1416.0 9 1707.5 10 1803.5 11 1902.0 12 1979.5 13 2060.5 14 2231.0 15 2443.5 16 2666.5 17 2854.5 18 3088.0 19 3385.0 20 3793.0 21 4319.5 22 4896.5 23 5743.5 24 6906.5 25 8392.0 26 10508.0 27 12952.0 28 15433.0 29 18140.0 30 21236.5 31 24480.0 32 27655.0 33 30950.5 34 34661.5 35 38241.0 36 41579.5 37 44628.0 38 46933.0 39 48739.5 40 50217.0 41 52165.0 42 54776.5 43 57479.5 44 60798.0 45 65121.0 46 71544.5 47 81670.0 48 93328.5 49 89918.0 50 74848.5 51 64818.0 52 57872.0 53 52855.5 54 48491.0 55 43695.0 56 38801.5 57 33886.5 58 29256.5 59 24667.5 60 20098.5 61 15982.0 62 12381.5 63 9275.5 64 6568.5 65 4545.5 66 3174.0 67 2222.0 68 1482.5 69 982.0 70 682.5 71 485.5 72 344.5 73 244.5 74 172.5 75 114.5 76 74.0 77 35.0 78 11.5 79 6.5 80 3.5 81 2.0 82 2.0 83 1.0 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026535236216321336 2 0.013355483128744513 3 2.3430672155692133E-4 4 0.0 5 0.0629699314184226 6 0.0 7 0.0 8 5.857668038923033E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 7.32208504865379E-4 16-17 0.0 18-19 4.1003676272461227E-4 20-21 0.0 22-23 0.0 24-25 1.1715336077846066E-4 26-27 8.786502058384549E-5 28-29 9.372268862276853E-4 30-31 0.0054476312761984206 32-33 0.006033398080090724 34-35 0.001786588751871525 36-37 0.09099887298466931 38-39 0.0845847264820486 40-41 0.08994449273766317 42-43 0.09389841866393621 44-45 0.09820380467254464 46-47 0.09217040659245392 48-49 0.08288600275076091 50-51 0.07717477641281095 52-53 0.0658694770976895 54-55 0.05397841097867574 56-57 0.07210789355914253 58-59 0.7237441745491352 60-61 0.3418242184113536 62-63 0.0032510057616022834 64-65 0.0024309322361530583 66-67 1.1003922294518864 68-69 2.3602301829232575 70-71 2.3183771447851527 72-73 1.8019358421335032 74-75 1.2750093136921818 76-77 0.7355766639877599 78-79 0.5575914206250835 80-81 0.04284884170472198 82-83 2.0501838136230614E-4 84-85 0.001786588751871525 86-87 0.0018451654322607554 88-89 2.9288340194615166E-5 90-91 0.32802941017968984 92-93 0.05974821399701493 94-95 0.9543019885611459 96-97 1.6457704122158152 98-99 0.6400673866131198 100-101 0.5728213575262833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1707164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.425730632344944 #Duplication Level Percentage of deduplicated Percentage of total 1 73.80163415627666 38.69104592503828 2 14.385379322771414 15.083280428394378 3 5.121547138026859 8.055025520371599 4 2.3476984082924583 4.923192174365016 5 1.3040091103195417 3.4181815179868034 6 0.7583163899596971 2.3853174476471595 7 0.49798245698251176 1.8274965904578926 8 0.3551176273892974 1.4893840861046999 9 0.23992233127665374 1.132029316097467 >10 1.0889708561198834 9.87955388953995 >50 0.06360552364965982 2.319671059197782 >100 0.029437250631046727 2.8688019223953996 >500 0.002014556306626798 0.7958645823515862 >1k 0.003693353228815796 4.303618366392052 >5k 5.595989740629994E-4 2.232868378162018 >10k+ 1.1191979481259989E-4 0.594668795497886 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10135 0.5936746557448493 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9232 0.5407799133533744 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9041 0.5295917673990314 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8056 0.47189373721563954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6203 0.3633511484543957 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5523 0.3235190057897191 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4948 0.28983741456591167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4428 0.2593775407635119 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4243 0.2485408548915043 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4132 0.2420388433682997 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3800 0.22259138547907525 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3648 0.2136877300599122 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3495 0.20472549796035996 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3212 0.1881482974102078 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2603 0.15247509905316653 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2547 0.14919480495136964 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2468 0.14456724720062045 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2299 0.13466778821484052 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2271 0.13302764116394208 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2179 0.12763858656813287 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1916 0.1122329196257653 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1871 0.10959696900824994 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1812 0.10614094486528534 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1770 0.10368072428893768 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1750 0.10250919068115306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.9288340194615162E-5 0.0 16-17 0.0 0.0 0.0 2.0501838136230614E-4 0.0 18-19 0.0 0.0 0.0 2.343067215569213E-4 0.0 20-21 0.0 0.0 0.0 4.1003676272461227E-4 0.0 22-23 0.0 0.0 0.0 6.736318244761488E-4 0.0 24-25 0.0 0.0 0.0 0.0014058403293415278 0.0 26-27 0.0 0.0 0.0 0.0021087604940122915 0.0 28-29 5.8576680389230325E-5 0.0 0.0 0.005037594513473808 0.0 30-31 5.8576680389230325E-5 0.0 0.0 0.012066796160181446 0.0 32-33 5.8576680389230325E-5 0.0 0.0 0.029346916875004396 0.0 34-35 5.8576680389230325E-5 0.0 0.0 0.05860596872942494 0.0 36-37 5.8576680389230325E-5 0.0 0.0 0.10206986557823385 0.0 38-39 5.8576680389230325E-5 0.0 0.0 0.16102729438999416 0.0 40-41 5.8576680389230325E-5 0.0 0.0 0.22677961812690522 0.0 42-43 8.786502058384549E-5 0.0 0.0 0.2949335857597747 0.0 44-45 1.1715336077846065E-4 0.0 0.0 0.37462715942932256 0.0 46-47 1.7573004116769097E-4 0.0 0.0 0.471249393731358 0.0 48-49 1.7573004116769097E-4 0.0 0.0 0.5694824867440973 0.0 50-51 1.7573004116769097E-4 0.0 0.0 0.6694143034881241 0.0 52-53 1.7573004116769097E-4 0.0 0.0 0.762199765224665 0.0 54-55 2.0501838136230614E-4 0.0 0.0 0.8660269312145757 0.0 56-57 2.343067215569213E-4 0.0 0.0 0.9652851161341265 0.0 58-59 2.343067215569213E-4 0.0 0.0 1.0414933773205153 0.0 60-61 2.343067215569213E-4 0.0 0.0 1.1100573817161092 2.9288340194615162E-5 62-63 2.343067215569213E-4 5.8576680389230325E-5 0.0 1.1675211051779444 5.8576680389230325E-5 64-65 2.343067215569213E-4 5.8576680389230325E-5 0.0 1.2269764357730129 5.8576680389230325E-5 66-67 2.928834019461516E-4 5.8576680389230325E-5 0.0 1.2763858656813287 5.8576680389230325E-5 68-69 2.928834019461516E-4 5.8576680389230325E-5 0.0 1.340468754027147 5.8576680389230325E-5 70-71 2.928834019461516E-4 5.8576680389230325E-5 0.0 1.440839895874093 5.8576680389230325E-5 72-73 2.928834019461516E-4 5.8576680389230325E-5 0.0 1.5818925422513597 5.8576680389230325E-5 74-75 2.928834019461516E-4 5.8576680389230325E-5 0.0 1.7677270607861928 5.8576680389230325E-5 76-77 2.928834019461516E-4 5.8576680389230325E-5 0.0 2.001594457240195 5.8576680389230325E-5 78-79 2.928834019461516E-4 5.8576680389230325E-5 0.0 2.3038208397084285 5.8576680389230325E-5 80-81 3.807484225299971E-4 1.1715336077846065E-4 0.0 2.6682556567500253 5.8576680389230325E-5 82-83 4.1003676272461227E-4 1.1715336077846065E-4 0.0 3.019569297384434 5.8576680389230325E-5 84-85 4.1003676272461227E-4 1.464417009730758E-4 0.0 3.356063037880368 5.8576680389230325E-5 86-87 4.1003676272461227E-4 1.7573004116769097E-4 0.0 3.6645278368100547 5.8576680389230325E-5 88-89 4.1003676272461227E-4 2.343067215569213E-4 0.0 3.9417126884118927 5.8576680389230325E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8955 0.0 26.190529 1 TACATGG 9135 0.0 25.408716 2 ACATGGG 9210 0.0 24.175215 3 CATGGGA 6540 0.0 21.422329 4 GAGTACT 6245 0.0 20.911901 12-13 AGTACTT 6515 0.0 19.791977 12-13 CATGGGG 2765 0.0 19.107807 4 GTACTTT 6885 0.0 18.865309 14-15 TTACGCG 90 1.05662926E-4 18.850271 62-63 ATGGGAT 2725 0.0 18.34963 5 ATGGGAG 2290 0.0 18.127384 5 CGCATAG 530 0.0 17.424286 66-67 GTATAGC 670 0.0 16.904016 1 ACTTTTT 7935 0.0 16.5472 16-17 GGTATCG 180 2.7621354E-8 16.157375 62-63 TACTTTT 7885 0.0 16.084103 14-15 GCGTTTA 710 0.0 16.043594 62-63 GCTTATA 1895 0.0 15.937649 1 AGTACAT 6820 0.0 15.633284 2 TCCGACG 95 0.003223352 15.348585 64-65 >>END_MODULE