##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939679_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 997707 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483234055689696 31.0 31.0 34.0 30.0 34.0 2 31.6207293323591 31.0 31.0 34.0 30.0 34.0 3 31.66735624787638 31.0 31.0 34.0 30.0 34.0 4 35.35155912507379 37.0 35.0 37.0 33.0 37.0 5 35.16038877145294 37.0 35.0 37.0 32.0 37.0 6 35.26100247868362 37.0 35.0 37.0 32.0 37.0 7 35.226725882448456 37.0 35.0 37.0 32.0 37.0 8 35.24618450106093 37.0 35.0 37.0 32.0 37.0 9 36.76238715374353 39.0 35.0 39.0 32.0 39.0 10-11 36.64135412500865 39.0 35.0 39.0 32.0 39.0 12-13 36.57750622176651 38.5 35.0 39.0 32.0 39.0 14-15 37.281104071636264 39.0 36.0 41.0 32.0 41.0 16-17 37.288594246607474 39.0 36.0 40.0 32.0 41.0 18-19 37.51579020694452 39.0 36.0 41.0 32.0 41.0 20-21 37.501470872711124 39.0 36.0 41.0 32.0 41.0 22-23 37.377923578766115 39.0 36.0 41.0 32.0 41.0 24-25 37.20083802158349 39.0 36.0 40.5 31.5 41.0 26-27 36.64219455210798 38.5 35.5 40.0 30.5 41.0 28-29 36.364108901711624 38.0 35.0 40.0 30.0 41.0 30-31 36.19386803941438 38.0 35.0 40.0 30.0 41.0 32-33 36.00646432269193 38.0 35.0 40.0 30.0 41.0 34-35 35.974990152419494 38.0 35.0 40.0 30.0 41.0 36-37 35.66847280814909 38.0 34.5 40.0 29.5 41.0 38-39 35.463563952142266 38.0 34.5 40.0 28.0 41.0 40-41 35.25692362587412 38.0 34.0 40.0 27.0 41.0 42-43 35.19280259635344 38.0 34.0 40.0 27.0 41.0 44-45 35.01380615751919 38.0 34.0 40.0 26.5 41.0 46-47 34.5609637899704 38.0 33.0 40.0 24.0 41.0 48-49 34.18740572131898 38.0 33.0 40.0 23.0 41.0 50-51 33.92994987506352 38.0 33.0 40.0 23.0 41.0 52-53 33.401377859431676 37.0 32.5 40.0 21.5 41.0 54-55 33.17618449103795 37.0 32.5 40.0 20.0 41.0 56-57 31.45921147190508 36.0 29.0 39.5 12.5 40.5 58-59 26.7834063507623 32.5 16.5 38.0 2.0 40.0 60-61 27.410609527646894 32.0 18.5 38.0 2.0 40.0 62-63 30.182426804663095 34.5 28.5 38.0 2.0 40.0 64-65 30.141547568574744 35.0 28.5 38.0 2.0 40.0 66-67 23.698263117328032 26.5 16.5 34.5 2.0 38.0 68-69 16.770313328462166 13.0 7.0 28.0 2.0 35.0 70-71 15.746659089291745 12.5 7.0 26.5 2.0 33.5 72-73 16.789434172557677 13.0 7.0 28.5 2.0 34.0 74-75 17.706262960969504 17.0 7.5 29.0 2.0 34.5 76-77 18.685443722455588 21.0 7.0 30.0 2.0 33.5 78-79 21.517550242706527 26.5 9.0 33.0 2.0 35.0 80-81 24.983542763556837 30.5 19.0 34.0 2.0 36.0 82-83 26.258530310000832 32.5 25.0 35.0 2.0 36.0 84-85 26.3301410133436 33.0 25.0 35.0 2.0 35.5 86-87 25.99384889551742 33.0 24.0 35.0 2.0 35.0 88-89 25.3474486998688 32.5 19.0 35.0 2.0 35.0 90-91 23.968110878243813 31.5 6.5 34.5 2.0 35.0 92-93 21.842593567049242 30.5 2.0 34.0 2.0 35.0 94-95 15.255523916340168 4.5 2.0 32.0 2.0 34.5 96-97 12.809439544876401 2.0 2.0 29.0 2.0 34.0 98-99 12.481088636242905 2.0 2.0 29.0 2.0 34.0 100-101 12.218405303360607 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28883164566243735 1101 2 0.3969685638745766 1101 3 0.4318422426637163 1101 4 0.3822782380604579 1101 5 0.49200594822772814 1101 6 0.347129727238098 1101 7 0.4225705061634599 1101 8 0.4646411897195222 1101 9 0.47063411455079773 1101 10-11 0.3973137373814808 1101 12-13 0.4695467473870636 1101 14-15 0.5544605552300581 1101 16-17 0.5454466521810915 1101 18-19 0.5488058913230276 1101 20-21 0.6654070152588005 1101 22-23 0.6563136400752754 1101 24-25 0.5893522793171613 1101 26-27 0.6697476117961116 1101 28-29 0.6270599740633784 1101 30-31 0.41924869890618766 1101 32-33 0.6896071725605495 1101 34-35 0.5759463492092181 1101 36-37 0.6386680438700978 1101 38-39 0.7620271386889641 1101 40-41 0.8013150360353549 1101 42-43 0.9554292984740158 1101 44-45 0.6575740495203704 1101 46-47 0.8490815851029154 1101 48-49 0.7135582321130016 1101 50-51 0.5652789781233878 1101 52-53 0.203128791068373 1101 54-55 -0.13576006782869854 1101 56-57 -2.459224150536791 1101 58-59 -9.873910135009424 1101 60-61 -9.904532516962032 1101 62-63 -4.060221541161557 1101 64-65 -4.996029156947959 1101 66-67 -9.162511090341441 1101 68-69 -4.408846326249282 1101 70-71 -4.555649543683172 1101 72-73 -4.514228928194633 1101 74-75 -6.588527184508656 1101 76-77 -7.830888987796872 1101 78-79 -10.896994836837646 1101 80-81 -8.16588047960439 1101 82-83 -4.551342701817763 1101 84-85 -3.5825074726374346 1101 86-87 -3.4127477185852513 1101 88-89 -3.6155518102498228 1101 90-91 -9.776968944346269 1101 92-93 -15.876343903100425 1101 94-95 -10.744865251914604 1101 96-97 -8.465799840596235 1101 98-99 -8.527754079289464 1101 100-101 -8.422930183911408 1102 1 0.3086053571176208 1102 2 0.26745644566919324 1102 3 0.3949161364111653 1102 4 0.21946278398349506 1102 5 0.3404418498052877 1102 6 0.187069424648314 1102 7 0.34603082875154456 1102 8 0.3402763282847232 1102 9 0.4547908397509772 1102 10-11 0.4717758982755669 1102 12-13 0.38243565678703817 1102 14-15 0.46316058830417717 1102 16-17 0.41763285181035315 1102 18-19 0.524534098936229 1102 20-21 0.5301315385683623 1102 22-23 0.6523985867336322 1102 24-25 0.6517716130658471 1102 26-27 0.5417107533764423 1102 28-29 0.653976158078585 1102 30-31 0.384510869030251 1102 32-33 0.6350202529922129 1102 34-35 0.2912573613543472 1102 36-37 0.3167922025951313 1102 38-39 0.32774926989324626 1102 40-41 0.5210872091591696 1102 42-43 0.5537863605687576 1102 44-45 0.7064983713085269 1102 46-47 0.8031047062234293 1102 48-49 0.817846330812344 1102 50-51 0.9738813271553894 1102 52-53 0.6380635273599182 1102 54-55 0.5216135390199526 1102 56-57 -2.8225772514282497 1102 58-59 -9.90134396753129 1102 60-61 -10.267951766102488 1102 62-63 -4.408712053502818 1102 64-65 -5.338848610686487 1102 66-67 -6.594743747578136 1102 68-69 -4.286658203705805 1102 70-71 -4.164477258261169 1102 72-73 -4.445622425667924 1102 74-75 -5.579217972211236 1102 76-77 -7.5024960056645895 1102 78-79 -9.903826566460523 1102 80-81 -6.167592642421379 1102 82-83 -4.022990485466842 1102 84-85 -3.8200366047877914 1102 86-87 -3.703136224173239 1102 88-89 -3.6729861811876674 1102 90-91 -9.246083096225725 1102 92-93 -12.570802064332938 1102 94-95 -9.804078703084121 1102 96-97 -8.764182285158618 1102 98-99 -8.92637111938607 1102 100-101 -8.895475583916024 1103 1 0.2873942208050835 1103 2 0.2816092408724593 1103 3 0.2303131520807078 1103 4 0.15983209167428214 1103 5 0.22052926652699512 1103 6 0.24601047544297927 1103 7 0.2692429738977893 1103 8 0.2951618908958977 1103 9 0.29873032370807096 1103 10-11 0.4443708026169304 1103 12-13 0.36446129234644076 1103 14-15 0.44197741043142 1103 16-17 0.5435774684710211 1103 18-19 0.3746861811992801 1103 20-21 0.3502987766356469 1103 22-23 0.41726869065748673 1103 24-25 0.5164242236269203 1103 26-27 0.45615385037941536 1103 28-29 0.2713258843771058 1103 30-31 0.41900226423794606 1103 32-33 0.4113399204731678 1103 34-35 0.34981165373206125 1103 36-37 0.1594502580623427 1103 38-39 0.42887669397734385 1103 40-41 0.6396618646005265 1103 42-43 0.5466167681678584 1103 44-45 0.41297203154591244 1103 46-47 0.2961413073159136 1103 48-49 0.5929859191476083 1103 50-51 0.9331581122144001 1103 52-53 0.8298473573632918 1103 54-55 0.8279961013410642 1103 56-57 -2.014786865170759 1103 58-59 -9.868688509869102 1103 60-61 -9.959402424698485 1103 62-63 -3.9966646453084316 1103 64-65 -5.185688200303186 1103 66-67 -6.015606801315421 1103 68-69 -3.976744723427572 1103 70-71 -4.101253906440318 1103 72-73 -4.125903687572103 1103 74-75 -4.97312734259882 1103 76-77 -6.329757115358364 1103 78-79 -8.55845537739973 1103 80-81 -4.716899098374608 1103 82-83 -2.8146843824889167 1103 84-85 -2.3435741162769617 1103 86-87 -2.187571527761122 1103 88-89 -2.3903776121588614 1103 90-91 -5.325156553531443 1103 92-93 -9.625295393332713 1103 94-95 -8.093153826246873 1103 96-97 -7.729132886090333 1103 98-99 -7.571230203024909 1103 100-101 -7.816994496053132 1104 1 0.19502656666155005 1104 2 0.2418087369986317 1104 3 0.2349540432581776 1104 4 0.17265994531393858 1104 5 0.23007759655645543 1104 6 0.29400880112869743 1104 7 0.36491680641897517 1104 8 0.25323690333479476 1104 9 0.332325202833637 1104 10-11 0.14856458475072998 1104 12-13 0.18637289329101492 1104 14-15 0.35754497927970164 1104 16-17 0.4020574359324627 1104 18-19 0.38505439213128767 1104 20-21 0.3183584675193245 1104 22-23 0.4091197359192549 1104 24-25 0.4413703217638485 1104 26-27 0.4150531861820923 1104 28-29 0.5138768640878908 1104 30-31 0.7107189497274788 1104 32-33 0.6494262266357467 1104 34-35 0.7445682660888124 1104 36-37 0.49158494953834264 1104 38-39 0.28916967923232306 1104 40-41 0.3505686992777868 1104 42-43 0.5044691639378485 1104 44-45 0.5054497600068899 1104 46-47 0.391502193122939 1104 48-49 0.578898874549246 1104 50-51 0.6268553456469803 1104 52-53 0.5962025252290886 1104 54-55 0.41015300512327 1104 56-57 -2.535313997749597 1104 58-59 -10.229777189293202 1104 60-61 -10.377423219365951 1104 62-63 -4.026646816230649 1104 64-65 -5.085663223798871 1104 66-67 -6.016365968580999 1104 68-69 -3.874636726207566 1104 70-71 -3.6106457631126965 1104 72-73 -3.9569721883500257 1104 74-75 -4.935425161251629 1104 76-77 -5.854480399383668 1104 78-79 -8.61662447129559 1104 80-81 -4.422898000593076 1104 82-83 -2.4190644518308027 1104 84-85 -1.9652309173889613 1104 86-87 -1.8665054752729482 1104 88-89 -1.9166977141972588 1104 90-91 -4.097183263582856 1104 92-93 -6.162736456680236 1104 94-95 -7.199510790841444 1104 96-97 -6.995659829124159 1104 98-99 -7.542681466263443 1104 100-101 -7.632263630227309 1105 1 0.22810755765043567 1105 2 0.1719667624976715 1105 3 0.21870463711940147 1105 4 0.2969794387600473 1105 5 0.2626873927354936 1105 6 0.11491193749480999 1105 7 0.17506131914122847 1105 8 0.07873516263991576 1105 9 0.08470843608674983 1105 10-11 0.13724666065593283 1105 12-13 0.16556341176106315 1105 14-15 0.26786803978733786 1105 16-17 0.5020354338607618 1105 18-19 0.4663087243976136 1105 20-21 0.41756834002413257 1105 22-23 0.381040322070028 1105 24-25 0.4635028482247421 1105 26-27 0.6157344715703843 1105 28-29 0.5445595319072325 1105 30-31 0.5566917917720815 1105 32-33 0.4856821360417314 1105 34-35 0.26958978138901557 1105 36-37 0.28982059756750544 1105 38-39 0.1838513112019129 1105 40-41 0.3677288548888953 1105 42-43 0.17491561128475297 1105 44-45 0.02309766065877028 1105 46-47 0.06161130782188451 1105 48-49 -0.09176467288565959 1105 50-51 0.07479221333080943 1105 52-53 0.08827496461630346 1105 54-55 -0.14976953979600438 1105 56-57 1.0775147677200039 1105 58-59 -8.747684052381903 1105 60-61 -10.371783484792715 1105 62-63 -4.283976134672667 1105 64-65 -5.669562672384121 1105 66-67 -6.291404520883621 1105 68-69 -3.759300600752921 1105 70-71 -3.58733281576969 1105 72-73 -2.09319081105928 1105 74-75 -4.4771215209796615 1105 76-77 -6.200893769633751 1105 78-79 -8.559152820867622 1105 80-81 -4.433991523027398 1105 82-83 -2.3366637726872064 1105 84-85 -1.9883472090468644 1105 86-87 -1.8927439193175921 1105 88-89 -2.1258858182806897 1105 90-91 -4.51687959605168 1105 92-93 -6.579278509843469 1105 94-95 -7.783315805756123 1105 96-97 -7.821932055110748 1105 98-99 -8.00979664418816 1105 100-101 -8.009138187265245 1106 1 0.16581027594979858 1106 2 0.2090032002861406 1106 3 0.3467562654994829 1106 4 0.20777745448025797 1106 5 0.17225467175029507 1106 6 0.14250913171714075 1106 7 0.28259491631172295 1106 8 0.11480621098812804 1106 9 0.21134056446305038 1106 10-11 0.3365035804487846 1106 12-13 0.30789956791469564 1106 14-15 0.5691752525947678 1106 16-17 0.5572805187341388 1106 18-19 0.4396159760363503 1106 20-21 0.3343490736553889 1106 22-23 0.6102232358634438 1106 24-25 0.6745704179350298 1106 26-27 0.5875699088055057 1106 28-29 0.48170620746194714 1106 30-31 0.6639524911454942 1106 32-33 0.6705860339499097 1106 34-35 0.5113901733694846 1106 36-37 0.7316302659157827 1106 38-39 0.6323081442715193 1106 40-41 0.5297492477969996 1106 42-43 0.04772204692671522 1106 44-45 0.31981415004974423 1106 46-47 0.37799100034264654 1106 48-49 0.5673298888976461 1106 50-51 0.4602366214607869 1106 52-53 0.4470287816798617 1106 54-55 0.4448121984874618 1106 56-57 1.4238830157916453 1106 58-59 -8.034200312452423 1106 60-61 -9.843622697293807 1106 62-63 -3.773284581452746 1106 64-65 -5.137879561737545 1106 66-67 -6.42014648361204 1106 68-69 -4.464739788229393 1106 70-71 -3.9879915457486454 1106 72-73 -4.56863755985416 1106 74-75 -4.961127876370256 1106 76-77 -6.338595517150434 1106 78-79 -8.418526320873553 1106 80-81 -4.449144361973474 1106 82-83 -2.4708852643055756 1106 84-85 -1.9724689797113193 1106 86-87 -1.9128914351386506 1106 88-89 -1.8208259911015787 1106 90-91 -3.904486692257322 1106 92-93 -5.889732652766412 1106 94-95 -6.837560984471821 1106 96-97 -6.869217261353528 1106 98-99 -6.545972860659754 1106 100-101 -7.208598971420346 1107 1 0.19278584251876651 1107 2 0.044947232269038295 1107 3 0.11456326837502573 1107 4 0.18746909487579444 1107 5 0.03675310266381615 1107 6 -0.011325974169267283 1107 7 0.12528617405328646 1107 8 0.1721078746609308 1107 9 0.14869508157527633 1107 10-11 0.26320123559035125 1107 12-13 0.14005194011289035 1107 14-15 0.107437572667898 1107 16-17 0.11454012124590207 1107 18-19 0.19860808802983598 1107 20-21 0.29114200245610533 1107 22-23 0.2001356358014803 1107 24-25 0.31226942621492526 1107 26-27 0.15902823081139417 1107 28-29 0.2697661723152365 1107 30-31 0.26959166178146177 1107 32-33 0.2717456909360081 1107 34-35 0.3474003747398626 1107 36-37 0.36218451917115857 1107 38-39 0.15152393957112764 1107 40-41 0.2198256862630643 1107 42-43 0.07180616514524729 1107 44-45 -0.06812978755201726 1107 46-47 0.020556792718501526 1107 48-49 -0.052066359041617716 1107 50-51 0.02673942057976575 1107 52-53 -0.02596231058694798 1107 54-55 -0.06626476215252097 1107 56-57 1.2306719949467535 1107 58-59 -8.500448795429453 1107 60-61 -10.076291190714311 1107 62-63 -4.074150631017208 1107 64-65 -5.436528680351042 1107 66-67 -6.397267776715754 1107 68-69 -3.976128104049211 1107 70-71 -3.95020574785355 1107 72-73 -2.47734842630382 1107 74-75 -4.786359135814781 1107 76-77 -6.6977496159079895 1107 78-79 -7.186028123861128 1107 80-81 -2.6586212544949 1107 82-83 -1.467593721984354 1107 84-85 -1.2296868288266545 1107 86-87 -0.9772727103304675 1107 88-89 -0.9963795313689836 1107 90-91 -0.6566920820360522 1107 92-93 -3.0293726297456196 1107 94-95 -5.6722929368996216 1107 96-97 -6.264149506978397 1107 98-99 -5.83300848235962 1107 100-101 -6.147795459621165 1108 1 -0.11361585952868225 1108 2 -0.0712089539718157 1108 3 -0.14416557166008914 1108 4 -0.0020329096376343614 1108 5 0.0645771802123889 1108 6 -0.21561093796236008 1108 7 -0.1451904934802286 1108 8 -0.11594341016498078 1108 9 -0.24390593385869153 1108 10-11 -0.11193075511749839 1108 12-13 -0.14351951463851975 1108 14-15 -0.23510253069064646 1108 16-17 -0.22258465426080676 1108 18-19 0.004593528473009201 1108 20-21 -0.06089445054124809 1108 22-23 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12.366230572496363 2316 100-101 11.19852038507418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 28.0 5 115.0 6 266.0 7 498.0 8 826.0 9 1396.0 10 2233.0 11 3524.0 12 5026.0 13 6757.0 14 8309.0 15 10860.0 16 14628.0 17 19378.0 18 20481.0 19 18773.0 20 19346.0 21 19761.0 22 19260.0 23 17329.0 24 16891.0 25 18855.0 26 26367.0 27 39310.0 28 55098.0 29 72660.0 30 87390.0 31 93188.0 32 91801.0 33 83214.0 34 74236.0 35 64673.0 36 47118.0 37 27548.0 38 9944.0 39 609.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.1125864198521 17.64879854349465 13.937156125182465 18.30145891147078 2 13.189653962659802 24.78434061252679 39.340923260741874 22.685082164071538 3 24.271879692013478 29.605114131818393 30.71977115502964 15.403235021138492 4 11.086621623382415 16.270207585994687 40.7758991367205 31.867271653902396 5 10.921525685368042 43.404969859262934 32.5770882221281 13.096416233240927 6 24.317961084767372 37.32227998801251 19.92137972370646 18.43837920351366 7 23.975074846623308 39.09424309942698 19.79278485567406 17.137897198275645 8 22.11085396179779 31.91208500427487 21.167575932828903 24.809485101098435 9 26.28707626587766 21.12544063537692 22.422815515978137 30.164667582767287 10-11 22.515728565600924 25.979220352267753 27.41862089771847 24.08643018441286 12-13 32.152074707303846 23.750560034158326 25.86696294603526 18.23040231250257 14-15 27.094052774354626 24.704858822863173 30.718537276467234 17.48255112631496 16-17 18.271747116137302 29.104185898264724 32.961079755880235 19.662987229717743 18-19 17.21885004199634 31.90774637017318 33.750523701370945 17.12287988645954 20-21 19.533590523069396 29.363530575609875 29.89314498144245 21.209733919878282 22-23 18.342336491078783 28.320330999478305 28.388236809359075 24.94909570008384 24-25 20.178218655376778 28.257193745257876 31.38952618353886 20.17506141582649 26-27 23.27479237370528 24.68161963544371 33.31948489835683 18.724103092494182 28-29 18.926070631724336 26.159287227794024 36.15320312484339 18.76143901563825 30-31 18.17445034428351 31.37666290088304 33.00094528854356 17.447941466289887 32-33 17.72866276896122 30.640505331864592 31.027903708700478 20.60292819047371 34-35 19.785650028691922 26.445584325439086 31.53395294524478 22.234812700624207 36-37 22.28138122018151 26.63434187356708 33.29973762234675 17.78453928390466 38-39 24.373027777917134 23.808589732655733 34.37468331569099 17.443699173736146 40-41 19.32846346166293 26.725490461704577 36.63436977408231 17.311676302550186 42-43 17.37391546932607 30.92962065761244 33.00916996650323 18.68729390655826 44-45 18.16187202503847 31.562551270813756 29.620745243699115 20.65483146044866 46-47 18.35602596989511 29.191297947904683 27.565595758845813 24.887080323354393 48-49 20.54078813428796 28.117451043251712 29.229560529369536 22.112200293090794 50-51 23.230028114859536 29.44359268170808 29.929281395428177 17.397097808004204 52-53 19.21070015363464 29.659811876564625 32.53819172579903 18.591296244001704 54-55 16.483672999603304 28.680063411280127 34.16169876432258 20.674564824793993 56-57 19.23698272021994 31.86837495893205 28.98651358819984 19.908128732648162 58-59 18.787734706053637 28.689067040255107 30.617062348679376 21.90613590501188 60-61 19.207776258776097 26.7551941294223 31.958340175557193 22.07868943624441 62-63 24.947313924810643 25.931321915552928 29.708982418262032 19.412381741374393 64-65 22.54884940104227 27.507047299446754 32.3824036785448 17.56169962096618 66-67 19.59996240324967 27.62814566288792 33.1187526052096 19.65313932865281 68-69 20.64126902244003 30.717255610007737 29.844983234459633 18.796492133092595 70-71 20.323020076910034 30.168504202425638 28.149989511331114 21.358486209333215 72-73 19.318757230073814 28.402714596824325 28.15823003676274 24.120298136339123 74-75 20.395752757557844 29.356153961004562 29.110054834137472 21.13803844730012 76-77 20.08289707750953 27.308970775095297 31.60213468869123 21.00599745870394 78-79 18.54968222294722 26.90682670591776 33.85735565992165 20.686135411213368 80-81 18.09409821582129 32.113461792060924 30.94667319840972 18.845766793708066 82-83 17.980866087671206 31.39695601405225 28.718609208132666 21.903568690143878 84-85 19.132289472048882 28.01762242584489 28.85779979272451 23.99228830938172 86-87 21.206361011242826 28.52251621975678 30.03814762509484 20.232975143905552 88-89 23.104039113707287 25.294462850547735 31.12924492323143 20.47225311251355 90-91 19.07658858121458 27.22208463089214 33.44504186432513 20.256284923568156 92-93 16.760634875277265 31.294356524455797 31.981069854089768 19.96393874617717 94-95 17.424603801521652 31.751198041209648 28.90289436939804 21.921303787870656 96-97 17.335334707180962 29.573533799373397 27.457199066002712 25.633932427442925 98-99 18.349570474376627 29.09454748213762 28.458633030446734 24.097249013039015 100-101 21.236190517619434 29.412041418457225 28.72159891309681 20.630169150826532 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6315.0 1 4523.5 2 2636.5 3 2523.0 4 2464.5 5 2764.5 6 3771.5 7 5470.5 8 6647.5 9 6803.0 10 6634.5 11 6242.5 12 6003.0 13 5908.0 14 5655.0 15 5299.5 16 4936.5 17 4535.0 18 4282.0 19 4176.0 20 4024.0 21 4019.0 22 4168.0 23 4340.0 24 4667.5 25 5276.5 26 6030.0 27 6996.5 28 8137.0 29 9280.0 30 10502.5 31 11869.5 32 13420.0 33 15127.5 34 16932.0 35 18420.5 36 19839.0 37 21015.0 38 21527.5 39 21902.0 40 22351.0 41 22979.5 42 24490.5 43 27970.0 44 33997.0 45 42887.0 46 55083.0 47 69885.5 48 85518.5 49 77684.5 50 52639.5 51 36163.0 52 24670.0 53 17754.5 54 13724.0 55 11239.5 56 9758.5 57 8702.5 58 7775.0 59 6875.0 60 6004.5 61 4984.0 62 3776.5 63 2647.0 64 1771.0 65 1193.5 66 820.5 67 581.5 68 395.0 69 313.5 70 262.0 71 219.0 72 187.0 73 166.5 74 120.5 75 80.5 76 57.5 77 26.0 78 10.0 79 5.5 80 2.5 81 1.0 82 2.0 83 1.0 84 0.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0251576865753172 2 0.006915858062537398 3 1.0022982699329563E-4 4 0.0 5 0.13841739107774126 6 0.0 7 0.0 8 0.00160367723189273 9 0.0 10-11 0.0 12-13 0.0 14-15 6.514938754564216E-4 16-17 0.0 18-19 1.0022982699329563E-4 20-21 0.0 22-23 1.5034474048994343E-4 24-25 0.0 26-27 1.0022982699329563E-4 28-29 0.0025057456748323907 30-31 0.01282941785514184 32-33 0.0026059755018256864 34-35 0.003959078166235177 36-37 0.10544177799694701 38-39 0.10544177799694701 40-41 0.11741924232264583 42-43 0.11957418360300169 44-45 0.11631671422571957 46-47 0.10940085616318218 48-49 0.108999936855209 50-51 0.10834844297975257 52-53 0.08634799595472419 54-55 0.0725162798296494 56-57 0.5461523272864679 58-59 1.5556671447629413 60-61 0.5107711983578346 62-63 0.0052620659171480205 64-65 0.12288176789378043 66-67 1.8942435003462939 68-69 2.852240186748214 70-71 2.7675960978523753 72-73 2.6130918195422104 74-75 1.9894618359899248 76-77 1.398907695345427 78-79 0.9220642934248232 80-81 0.052670774084976855 82-83 2.0045965398659125E-4 84-85 1.0022982699329563E-4 86-87 4.009193079731825E-4 88-89 5.0114913496647813E-5 90-91 0.2414035383133525 92-93 0.38513311022173846 94-95 1.980190576993045 96-97 2.40977561548631 98-99 1.403768841954602 100-101 0.9231668215217493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 997707.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.079498421347964 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14491683343155 30.929436524498037 2 12.120402537142704 9.473185036327402 3 3.882625736174684 4.551931989825706 4 1.7433216588344402 2.7251254405728886 5 0.9078471535631683 1.7739105702248534 6 0.5154623401096163 1.2086405823946846 7 0.316704679985992 0.866366202908226 8 0.22707540990059422 0.709919449818977 9 0.16581637325928675 0.5832018627317948 >10 0.8230756614238854 5.861435848036321 >50 0.07174704239828425 1.9427879076749723 >100 0.05708893770879918 4.820181180040441 >500 0.007200406557801501 1.950121253404174 >1k 0.011572081967895269 9.140797673067945 >5k 0.0028287311477077325 7.664112195226104 >10k+ 0.0023144163935790536 15.798846283247453 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33635 3.3712302309194984 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 32021 3.2094592901523193 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 19678 1.9723225355740714 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13724 1.3755541456559892 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 13381 1.341175314997289 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 12846 1.2875523575558756 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 11159 1.1184646394181859 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10739 1.0763681120810018 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 10026 1.0049042454347819 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9872 0.9894688520778145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9291 0.9312353225947096 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 8122 0.8140666548395471 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7313 0.732980724801971 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6954 0.6969982169113778 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6897 0.6912851167727599 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6654 0.6669292688133891 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5580 0.5592824346225895 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5397 0.5409403762828165 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5096 0.5107711983578346 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5087 0.5098691299148949 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4564 0.45744893039740125 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4352 0.4362002070748226 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3763 0.3771648389757714 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3361 0.33687244852446663 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3117 0.31241637073810247 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2961 0.29678051772714836 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2950 0.29567798963022207 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2819 0.2825478822941004 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2800 0.28064351558122774 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2779 0.27853868921436853 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2661 0.26671156962915965 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2628 0.26340398533838094 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2328 0.2333350372403922 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2271 0.22762193710177436 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2186 0.21910240180734422 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2078 0.20827758049206832 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2023 0.20276494000743706 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1995 0.19995850485162478 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1989 0.19935712588966503 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1865 0.18692862734249635 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1828 0.1832201237437444 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1807 0.1811152973768852 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1759 0.176304265681207 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1688 0.169187947964683 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1680 0.16838610934873666 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1656 0.16598059350089756 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1596 0.15996680388129983 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1554 0.1557571511475814 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1527 0.1530509458187624 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1510 0.1513470387598764 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1488 0.1491419825660239 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1472 0.14753830533413118 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1443 0.14463164035132559 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1414 0.14172497536852002 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1332 0.13350612955506977 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1304 0.1306996943992575 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1273 0.12759256976246533 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1258 0.1260891223575659 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1256 0.12588866270357932 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1215 0.12177923979685419 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1150 0.11526430104228998 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1148 0.11506384138830338 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1051 0.10534154816995371 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1043 0.10453970955400735 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1015 0.10173327439819506 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0022982699329563E-4 0.0 5 0.0 0.0 0.0 1.0022982699329563E-4 0.0 6 0.0 0.0 0.0 1.0022982699329563E-4 0.0 7 0.0 0.0 0.0 1.0022982699329563E-4 0.0 8 0.0 0.0 0.0 1.0022982699329563E-4 0.0 9 0.0 0.0 0.0 1.0022982699329563E-4 0.0 10-11 0.0 0.0 0.0 2.5057456748323905E-4 0.0 12-13 0.0 0.0 0.0 5.51264048463126E-4 0.0 14-15 0.0 0.0 0.0 7.517237024497172E-4 1.0022982699329563E-4 16-17 0.0 0.0 0.0 0.0018041368858793213 1.0022982699329563E-4 18-19 0.0 0.0 0.0 0.002706205328818982 1.0022982699329563E-4 20-21 0.0 0.0 0.0 0.004059307993228473 1.0022982699329563E-4 22-23 0.0 0.0 0.0 0.009020684429396606 1.0022982699329563E-4 24-25 0.0 0.0 0.0 0.013982060865564739 1.0022982699329563E-4 26-27 0.0 0.0 0.0 0.016437691626900484 1.0022982699329563E-4 28-29 0.0 0.0 0.0 0.021900217198035095 1.0022982699329563E-4 30-31 0.0 0.0 0.0 0.0333264174752708 1.0022982699329563E-4 32-33 0.0 0.0 0.0 0.05662985225121203 1.0022982699329563E-4 34-35 0.0 0.0 0.0 0.10514108851596712 1.0022982699329563E-4 36-37 0.0 0.0 0.0 0.1740992094873545 1.0022982699329563E-4 38-39 0.0 0.0 0.0 0.2494219244728162 1.0022982699329563E-4 40-41 0.0 0.0 0.0 0.3284531430570298 1.0022982699329563E-4 42-43 0.0 0.0 0.0 0.4039262027829814 1.0022982699329563E-4 44-45 0.0 0.0 0.0 0.4823560424052352 1.0022982699329563E-4 46-47 0.0 0.0 0.0 0.5867454072187526 1.0022982699329563E-4 48-49 0.0 0.0 0.0 0.6970984467383711 1.0022982699329563E-4 50-51 0.0 0.0 0.0 0.7936197701329147 1.0022982699329563E-4 52-53 0.0 0.0 0.0 0.8811705240115585 1.0022982699329563E-4 54-55 0.0 0.0 0.0 0.9647120848104704 1.0022982699329563E-4 56-57 0.0 0.0 0.0 1.032066528549965 1.0022982699329563E-4 58-59 0.0 0.0 0.0 1.1019768328777888 1.0022982699329563E-4 60-61 0.0 0.0 0.0 1.1777505820847203 1.0022982699329563E-4 62-63 5.0114913496647813E-5 0.0 0.0 1.2331776764120126 1.0022982699329563E-4 64-65 1.0022982699329563E-4 0.0 0.0 1.2710645510154785 1.0022982699329563E-4 66-67 1.0022982699329563E-4 0.0 0.0 1.3031882105668298 1.0022982699329563E-4 68-69 1.0022982699329563E-4 0.0 0.0 1.347990943232833 1.0022982699329563E-4 70-71 1.0022982699329563E-4 0.0 0.0 1.4219104406403886 1.0022982699329563E-4 72-73 1.0022982699329563E-4 0.0 0.0 1.534969685488826 1.0022982699329563E-4 74-75 1.0022982699329563E-4 0.0 0.0 1.667724091341446 1.0022982699329563E-4 76-77 1.0022982699329563E-4 0.0 0.0 1.8472357114864384 1.0022982699329563E-4 78-79 1.0022982699329563E-4 0.0 0.0 2.129583134126552 1.0022982699329563E-4 80-81 1.0022982699329563E-4 0.0 0.0 2.474874888118456 1.0022982699329563E-4 82-83 1.0022982699329563E-4 0.0 0.0 2.8215698596882652 1.0022982699329563E-4 84-85 1.0022982699329563E-4 5.0114913496647813E-5 0.0 3.212616529702608 1.0022982699329563E-4 86-87 1.0022982699329563E-4 1.0022982699329563E-4 0.0 3.539816799922222 1.0022982699329563E-4 88-89 1.0022982699329563E-4 1.0022982699329563E-4 0.0 3.755060353390324 1.0022982699329563E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 545 0.0 37.11785 2 GTCGGGA 435 0.0 34.60761 2 CGGGAGT 450 0.0 34.494267 4 TGCGACG 30 0.0034876307 32.48483 66-67 ACGCTAG 30 0.0034876307 32.48483 66-67 TCGGGAG 490 0.0 30.718454 3 ACCGACG 40 3.8726558E-4 30.452156 64-65 GGTCGGG 505 0.0 29.819496 1 ACCTGGG 715 0.0 28.28837 3 CGGCTTG 570 0.0 26.409487 74-75 CGTGTAC 700 0.0 26.039085 76-77 ACTGCGG 270 0.0 25.230566 62-63 GCAACTC 515 0.0 25.067379 68-69 CAAGCGG 165 0.0 24.753838 54-55 GCGTTTG 305 0.0 24.728317 62-63 GTAGCCC 305 0.0 24.675482 3 GCTTATA 3805 0.0 24.36426 1 GCGACGC 80 1.493303E-6 24.357927 68-69 GAACGCG 50 0.0014276997 24.357924 68-69 ATGGGAG 1855 0.0 24.342926 5 >>END_MODULE