FastQCFastQC Report
Fri 27 May 2016
SRR939677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4456656
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106120.23811575315662686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70930.15915520515830703No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59620.13377743312474644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50860.11412143993164382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA197300.026.1699161
GTACATG119350.024.5734771
TACATGG118950.023.6589582
CAACGCA223000.023.2390465
ATCAACG224050.023.0882933
TCAACGC224600.023.0526264
TATCAAC224400.022.925132
AACGCAG229100.022.69836
ACATGGG122200.021.8648993
GAGTACT98200.020.8744112-13
AGAGTAC152550.020.8403310-11
ACGCAGA247150.020.6376677
GGTATCA143350.020.0951611
CGCAGAG248600.020.078778
AGTACTT107050.019.10436412-13
GTATAGG13850.018.845751
GTACTTT112200.017.95358514-15
GCAGAGT255700.017.9445639
CATGGGG75150.017.3032234
CATGGGA59850.016.0967184
Produced by FastQC (version 0.11.5)