##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939677_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4456656 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.955649033714966 31.0 30.0 33.0 25.0 34.0 2 30.12187142108343 31.0 30.0 34.0 25.0 34.0 3 30.166310121310687 31.0 30.0 34.0 25.0 34.0 4 33.73123436047117 35.0 33.0 37.0 28.0 37.0 5 33.717114356593825 35.0 33.0 37.0 28.0 37.0 6 33.8018945146316 35.0 35.0 37.0 28.0 37.0 7 33.60036381538086 35.0 33.0 37.0 28.0 37.0 8 33.59769073493669 35.0 33.0 37.0 28.0 37.0 9 34.84154868583081 37.0 34.0 39.0 27.0 39.0 10-11 34.729024183154365 37.0 34.0 39.0 27.0 39.0 12-13 34.56835035057675 37.0 34.0 39.0 27.0 39.0 14-15 35.23180474328734 38.0 33.5 40.0 26.5 41.0 16-17 34.930637231143706 38.0 33.0 40.0 25.5 41.0 18-19 34.87434255639206 38.0 33.0 40.0 25.0 41.0 20-21 34.98997275086971 38.0 34.0 40.0 25.0 41.0 22-23 34.82993841122133 38.0 33.0 40.0 25.0 41.0 24-25 34.70324353057539 38.0 33.0 40.0 25.0 41.0 26-27 34.83861722780489 38.0 33.5 40.0 25.0 41.0 28-29 34.70869896622041 38.0 33.0 40.0 24.0 41.0 30-31 34.49016897422642 38.0 33.0 40.0 24.0 41.0 32-33 34.263269590473215 38.0 33.0 40.0 24.0 41.0 34-35 34.02968313013165 38.0 33.0 40.0 23.0 41.0 36-37 33.749142069749155 38.0 32.0 40.0 20.5 41.0 38-39 33.58873626324311 38.0 32.0 40.0 20.0 41.0 40-41 33.31468998280325 37.5 31.5 40.0 18.5 41.0 42-43 32.999877711001254 37.0 31.0 40.0 16.5 41.0 44-45 32.75676190848026 37.0 31.0 40.0 15.5 41.0 46-47 32.33230195464941 37.0 30.0 40.0 13.5 41.0 48-49 31.981771422339982 37.0 30.0 39.0 9.0 41.0 50-51 31.378981909305992 35.5 29.5 38.5 9.0 40.0 52-53 31.439253332543505 36.0 30.0 39.0 8.5 40.0 54-55 31.460006673164813 36.0 30.0 39.0 8.0 40.0 56-57 31.301283406213088 36.0 29.0 39.0 7.0 40.0 58-59 30.879950236230933 35.5 28.5 39.0 2.0 40.0 60-61 30.43700220524088 35.0 28.0 39.0 2.0 40.0 62-63 30.00950017232652 35.0 28.0 38.0 2.0 40.0 64-65 29.374767202135413 34.0 26.5 38.0 2.0 40.0 66-67 28.815200791804436 34.0 26.0 37.5 2.0 39.5 68-69 28.27388147077091 33.5 26.0 37.0 2.0 39.0 70-71 27.703704750826628 33.0 25.0 36.0 2.0 39.0 72-73 26.94634587008735 32.0 22.5 36.0 2.0 38.5 74-75 26.475718341285486 32.0 22.0 35.0 2.0 38.0 76-77 26.08182727138913 32.0 21.5 35.0 2.0 37.0 78-79 25.48802588757131 31.0 19.5 35.0 2.0 36.5 80-81 24.800394623233203 31.0 18.0 34.0 2.0 36.0 82-83 24.12302991301101 30.5 12.0 34.0 2.0 35.5 84-85 23.443034194247886 30.0 7.0 34.0 2.0 35.0 86-87 22.759664196653276 29.5 2.0 34.0 2.0 35.0 88-89 21.947062775318535 29.0 2.0 34.0 2.0 35.0 90-91 20.723031797832277 28.0 2.0 33.5 2.0 34.5 92-93 19.177879558126094 25.0 2.0 32.0 2.0 34.0 94-95 18.80735051123533 25.0 2.0 32.0 2.0 34.0 96-97 18.11583449563978 24.5 2.0 32.0 2.0 34.0 98-99 17.091940795968995 21.0 2.0 32.0 2.0 34.0 100-101 15.47455614703042 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1542804148461627 1101 2 1.1133480611358415 1101 3 1.152549483885437 1101 4 1.3323981927516968 1101 5 1.023296778833668 1101 6 1.0992599752774055 1101 7 1.0292512064291515 1101 8 1.0754245337648882 1101 9 1.3600716367203844 1101 10-11 1.1961822607053492 1101 12-13 1.2736186413151813 1101 14-15 1.409799331026882 1101 16-17 1.2929855542079594 1101 18-19 1.405478084073657 1101 20-21 1.485400352098786 1101 22-23 1.530845982427195 1101 24-25 1.2883619774574129 1101 26-27 1.3055091863052795 1101 28-29 1.4877826708332051 1101 30-31 1.6616174348145094 1101 32-33 1.5555396304505749 1101 34-35 1.5549778472757652 1101 36-37 1.6163260570448656 1101 38-39 1.679863216959518 1101 40-41 1.7580487340663495 1101 42-43 1.8869785876633074 1101 44-45 1.6325525880052965 1101 46-47 1.418145864678479 1101 48-49 1.4003298024319264 1101 50-51 1.362272188562006 1101 52-53 1.4266201610452995 1101 54-55 1.5117757008084958 1101 56-57 1.2756941899881973 1101 58-59 1.4206497961128584 1101 60-61 1.1282410460553933 1101 62-63 1.2837958357811772 1101 64-65 1.1887114335687556 1101 66-67 1.130435609451908 1101 68-69 1.0695748376645433 1101 70-71 1.0991294570732286 1101 72-73 1.305731528098761 1101 74-75 0.9869076760762177 1101 76-77 0.5833135007831238 1101 78-79 0.6707859568953438 1101 80-81 0.45121043625199775 1101 82-83 0.5147641157653418 1101 84-85 0.2172165983456864 1101 86-87 -0.08407051845114566 1101 88-89 -0.3704991107080957 1101 90-91 -3.703987283256623 1101 92-93 -9.009565383593863 1101 94-95 -7.330715080189403 1101 96-97 -3.909290696866501 1101 98-99 -2.6750600254666423 1101 100-101 -2.2736937762603286 1102 1 1.0456356962672508 1102 2 0.9405550623599339 1102 3 0.9186210689602312 1102 4 1.048216437002651 1102 5 1.0192826304086537 1102 6 0.9811426387796658 1102 7 0.9841363403168799 1102 8 0.9263641416420185 1102 9 1.1809498641702731 1102 10-11 1.0104180486943761 1102 12-13 0.9720233444983037 1102 14-15 1.2176007887999631 1102 16-17 1.3446572560813834 1102 18-19 1.1959923145087359 1102 20-21 1.2513666489138515 1102 22-23 1.3259087407736274 1102 24-25 1.4374025777474557 1102 26-27 1.343973942951017 1102 28-29 1.3853159540987363 1102 30-31 1.4985306941303236 1102 32-33 1.4539372107193245 1102 34-35 1.4197946408765603 1102 36-37 1.3624126646034966 1102 38-39 1.3889305689420866 1102 40-41 1.4393042163772236 1102 42-43 1.290449313504368 1102 44-45 1.2965633230096003 1102 46-47 1.4333582750265705 1102 48-49 1.3962127257489243 1102 50-51 1.3082329758457156 1102 52-53 1.3121386217835038 1102 54-55 1.337710285636458 1102 56-57 1.3465221869463697 1102 58-59 1.4097152107485336 1102 60-61 1.2630557167273402 1102 62-63 1.1639554298265473 1102 64-65 1.1923605326327262 1102 66-67 1.2112232965075194 1102 68-69 1.040877806428739 1102 70-71 1.0672087170882456 1102 72-73 0.7971133380849373 1102 74-75 0.7078894432390932 1102 76-77 0.3820519082131817 1102 78-79 0.39604045358366946 1102 80-81 0.2135865267739412 1102 82-83 -0.09899402932477486 1102 84-85 -0.10940104607864143 1102 86-87 -0.565631127533269 1102 88-89 -1.0218580908955595 1102 90-91 -5.328429692616734 1102 92-93 -10.386399896087767 1102 94-95 -8.802731359481449 1102 96-97 -5.450102528885438 1102 98-99 -4.069308883574436 1102 100-101 -3.4218713668208363 1103 1 0.924542492515048 1103 2 1.0096820365674972 1103 3 0.8450799323473461 1103 4 0.9650687665586162 1103 5 0.9091158552340914 1103 6 0.7758529688161033 1103 7 0.9230996921400916 1103 8 0.7582782284930616 1103 9 0.8457521757724891 1103 10-11 0.8543221505999341 1103 12-13 0.8175068188577441 1103 14-15 0.8443736250152014 1103 16-17 0.8601247846473044 1103 18-19 0.8382892489517033 1103 20-21 0.9345418070271734 1103 22-23 0.9834903688427374 1103 24-25 0.813011461956819 1103 26-27 0.8139033739786683 1103 28-29 0.9418471984801897 1103 30-31 1.0164461171394947 1103 32-33 1.083802009547746 1103 34-35 0.9916454426994932 1103 36-37 1.0967898976164676 1103 38-39 1.042386325383788 1103 40-41 0.8699628409103894 1103 42-43 0.8481983583190669 1103 44-45 0.8721546056343215 1103 46-47 0.9153924611584969 1103 48-49 0.8088915140530162 1103 50-51 0.7861657494456615 1103 52-53 0.8918004229256837 1103 54-55 0.8980130008777785 1103 56-57 0.775988462591922 1103 58-59 0.6442987133319775 1103 60-61 0.48664805736800787 1103 62-63 0.6940824724171293 1103 64-65 0.5968911446633243 1103 66-67 0.6349477354722168 1103 68-69 0.5768586404864564 1103 70-71 0.6748029972967622 1103 72-73 0.6240072596283532 1103 74-75 0.5456606830821613 1103 76-77 0.2724494850467529 1103 78-79 0.27865952583865905 1103 80-81 0.03527226171725317 1103 82-83 0.06247925212283789 1103 84-85 -0.1412731682931856 1103 86-87 -0.5802076599148052 1103 88-89 -0.945261776356201 1103 90-91 -2.4681930617534 1103 92-93 -8.96902982766889 1103 94-95 -5.548114737233867 1103 96-97 -3.023428848050255 1103 98-99 -2.0890600193216464 1103 100-101 -1.5876237414020231 1104 1 0.8108993689199586 1104 2 0.844291364621256 1104 3 0.851947746065413 1104 4 0.8788620992657599 1104 5 0.9254137985160114 1104 6 0.8513971750710922 1104 7 0.8913564648471493 1104 8 0.8369740629627742 1104 9 0.9173500921585998 1104 10-11 0.834076172805382 1104 12-13 0.7808796499826371 1104 14-15 0.9865625154255397 1104 16-17 0.9672164644506651 1104 18-19 0.7816705137788134 1104 20-21 0.9431009585606986 1104 22-23 0.9279129973814833 1104 24-25 0.8337236604305147 1104 26-27 0.8155547264453702 1104 28-29 0.8707125085873173 1104 30-31 0.9621371724169734 1104 32-33 0.9864531041843918 1104 34-35 0.8557032964066806 1104 36-37 0.8297657110071057 1104 38-39 0.6565127860278324 1104 40-41 0.6822398094249422 1104 42-43 0.7798987852569681 1104 44-45 0.8307061137421101 1104 46-47 0.7157747786187727 1104 48-49 0.6034365600003326 1104 50-51 0.6782058736114926 1104 52-53 0.7457396493449835 1104 54-55 0.8033729378593542 1104 56-57 0.8484100158642853 1104 58-59 0.6485066761878535 1104 60-61 0.7022405940272165 1104 62-63 0.5269294037868093 1104 64-65 0.5887167106540474 1104 66-67 0.5900227344065705 1104 68-69 0.49056707505627983 1104 70-71 0.5116375948838368 1104 72-73 0.5175663319456305 1104 74-75 0.40409484267877716 1104 76-77 0.37550950531857197 1104 78-79 0.4185568031004614 1104 80-81 0.3264332410338895 1104 82-83 0.23610952288023057 1104 84-85 0.18893904451487487 1104 86-87 0.09088488504324843 1104 88-89 -0.1571026688065551 1104 90-91 -0.6116597386804585 1104 92-93 -3.9964769875963277 1104 94-95 -1.4148381001073211 1104 96-97 -0.6487417994178841 1104 98-99 -0.4922031742260202 1104 100-101 -0.20181746513570253 1105 1 1.0909101744642449 1105 2 1.1056702486268044 1105 3 1.102235056671315 1105 4 0.9956376089106485 1105 5 0.822624311003942 1105 6 0.9389374150988203 1105 7 0.9645163092674451 1105 8 0.9918029082299711 1105 9 1.180295565726908 1105 10-11 0.9981053416237913 1105 12-13 1.0054029744033102 1105 14-15 1.2522979378432026 1105 16-17 1.386292942708117 1105 18-19 1.146845579160562 1105 20-21 1.0753767251098765 1105 22-23 1.1468287438666778 1105 24-25 1.2215985936647584 1105 26-27 1.152535140674054 1105 28-29 1.1855348466688795 1105 30-31 1.2436368391823436 1105 32-33 1.3161642641755051 1105 34-35 1.3138934637341748 1105 36-37 1.4009582781272556 1105 38-39 1.4787211339585298 1105 40-41 1.1400058027535351 1105 42-43 1.4855046017228375 1105 44-45 1.383845248312511 1105 46-47 1.481193439404187 1105 48-49 1.3854138273495806 1105 50-51 1.2809293992124928 1105 52-53 1.2562258457141695 1105 54-55 1.4637549325829546 1105 56-57 1.4451963403734034 1105 58-59 1.2892187171933749 1105 60-61 1.2364807906333226 1105 62-63 1.22474570338278 1105 64-65 1.5372278587529138 1105 66-67 1.414115295627191 1105 68-69 1.2472940948910818 1105 70-71 1.3496143345377867 1105 72-73 1.4065135248574023 1105 74-75 1.3501934065032053 1105 76-77 0.9767735042255303 1105 78-79 0.892528060576339 1105 80-81 0.954032282458904 1105 82-83 0.9785343880462527 1105 84-85 1.0444765644234444 1105 86-87 0.7963044657072338 1105 88-89 0.581882414341802 1105 90-91 -0.468033187760053 1105 92-93 -6.804912973134384 1105 94-95 -2.443313328205985 1105 96-97 -0.7383983193671817 1105 98-99 -0.05770549549813353 1105 100-101 0.19159141846815508 1106 1 0.8302678536872214 1106 2 0.7812531739517787 1106 3 0.8279437605248994 1106 4 0.869762337493313 1106 5 0.717040892631772 1106 6 0.7194308073008742 1106 7 0.8089477531143245 1106 8 0.9135543225867053 1106 9 1.0145041329304618 1106 10-11 0.7297661421444985 1106 12-13 0.928243566065099 1106 14-15 0.9903208816593789 1106 16-17 1.019898466844971 1106 18-19 0.9302662299081916 1106 20-21 0.8803952568616609 1106 22-23 0.9732824059908296 1106 24-25 0.8948992447059041 1106 26-27 0.8138457013202114 1106 28-29 0.9006516608785304 1106 30-31 0.8769461166184556 1106 32-33 0.9057393862239991 1106 34-35 0.9466091535387449 1106 36-37 1.0778432406897878 1106 38-39 0.9498962173356489 1106 40-41 0.9839986856236749 1106 42-43 0.9048657670340887 1106 44-45 0.827709990404415 1106 46-47 0.9696029039021496 1106 48-49 0.9156465031450409 1106 50-51 0.6897649401657269 1106 52-53 0.8471221862097309 1106 54-55 0.9573439029497983 1106 56-57 0.8361147328232725 1106 58-59 0.7356268361745428 1106 60-61 0.7299243893165901 1106 62-63 0.6998506175185426 1106 64-65 0.8067928855634818 1106 66-67 0.6824920851278335 1106 68-69 0.8010474392121978 1106 70-71 0.8517862203171624 1106 72-73 0.8786112051025192 1106 74-75 0.8819448092739783 1106 76-77 0.8426020378581072 1106 78-79 0.5736649759211652 1106 80-81 0.6418819131047613 1106 82-83 0.7569972754310577 1106 84-85 0.5821536414233819 1106 86-87 0.5173491426061965 1106 88-89 0.4369190395096858 1106 90-91 0.03366158152411103 1106 92-93 -3.786196167730072 1106 94-95 -1.3795063267040568 1106 96-97 -0.4657369110982259 1106 98-99 -0.3075539768162123 1106 100-101 -0.13906254234218807 1107 1 0.7343341214760883 1107 2 0.7115943574711991 1107 3 0.6858723673880647 1107 4 0.6912171792431607 1107 5 0.5114581038805 1107 6 0.6079745192391925 1107 7 0.5540192120154046 1107 8 0.5499098374451918 1107 9 0.6715728143109772 1107 10-11 0.6193142477487044 1107 12-13 0.6026684909769386 1107 14-15 0.8027811308099402 1107 16-17 0.8737440796081728 1107 18-19 0.816031982435149 1107 20-21 0.7609257304388208 1107 22-23 0.803247385433707 1107 24-25 0.7013504035953844 1107 26-27 0.6079288565394378 1107 28-29 0.7557154481203483 1107 30-31 0.665931414454306 1107 32-33 0.5942325932289592 1107 34-35 0.6085563318733875 1107 36-37 0.6096828521679214 1107 38-39 0.573426826892451 1107 40-41 0.6154722867965745 1107 42-43 0.5580503457012611 1107 44-45 0.44332867238991014 1107 46-47 0.607695027383194 1107 48-49 0.6193076782360976 1107 50-51 0.6384307441442694 1107 52-53 0.782346537261148 1107 54-55 0.7087685389127323 1107 56-57 0.6408814117546449 1107 58-59 0.6825683819176511 1107 60-61 0.7581412623653314 1107 62-63 0.5778299838458842 1107 64-65 0.6664767206683848 1107 66-67 0.4780423660301807 1107 68-69 0.4920276505693266 1107 70-71 0.48920092192961206 1107 72-73 0.5353017264060931 1107 74-75 0.3921808038566077 1107 76-77 0.16343970505930727 1107 78-79 0.10411275519303942 1107 80-81 0.05364836515262539 1107 82-83 0.10485545812989372 1107 84-85 0.1580940089897105 1107 86-87 0.06036911443603188 1107 88-89 0.14056259805870397 1107 90-91 -0.17987222346026854 1107 92-93 -3.8302946982408876 1107 94-95 -1.5301264133109918 1107 96-97 -0.7097122261224307 1107 98-99 -0.47085653177277464 1107 100-101 -0.32803942059940816 1108 1 0.6086063875492655 1108 2 0.5409747578635837 1108 3 0.30720113760983025 1108 4 -0.011162711298105421 1108 5 -0.9127178935350813 1108 6 0.069776753390137 1108 7 0.3145881432892921 1108 8 0.5141452261322712 1108 9 0.5772468720395807 1108 10-11 0.4905032021626283 1108 12-13 0.43808270328703003 1108 14-15 0.48635992921273896 1108 16-17 0.5988228459628075 1108 18-19 0.6421194400497754 1108 20-21 0.5908879214556961 1108 22-23 0.48875173128156746 1108 24-25 0.5316110716776947 1108 26-27 0.5699176497496055 1108 28-29 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0.3898303309683797 2311 80-81 0.4206104005399247 2311 82-83 0.49420744471362354 2311 84-85 0.5008225702789133 2311 86-87 0.7566533434087255 2311 88-89 0.7281963053922205 2311 90-91 1.5710614928760513 2311 92-93 2.620069077827985 2311 94-95 1.7754247362213853 2311 96-97 1.2454952675598996 2311 98-99 1.147128578009088 2311 100-101 1.2826710262118208 2312 1 0.1315586534242179 2312 2 -0.04460405167962733 2312 3 -0.10339845552144666 2312 4 -0.19753556792800708 2312 5 -0.0407432021461247 2312 6 -0.06032384890275466 2312 7 0.023966558093498236 2312 8 -0.7463084629821992 2312 9 -0.06457750482263691 2312 10-11 -0.04684495258742061 2312 12-13 -0.02625980337839451 2312 14-15 -0.40464315773552784 2312 16-17 -0.034806082535844496 2312 18-19 0.03022191787196249 2312 20-21 0.26321047178974055 2312 22-23 0.0494287535465503 2312 24-25 0.22806442828744622 2312 26-27 0.24483752534968772 2312 28-29 0.1328222697444872 2312 30-31 0.26854635737110755 2312 32-33 0.24298952684532793 2312 34-35 0.08527542710464786 2312 36-37 0.2615569092007064 2312 38-39 0.3327527395958043 2312 40-41 0.46661306165717065 2312 42-43 0.46693780984365674 2312 44-45 0.2980440935143349 2312 46-47 0.5288242997947847 2312 48-49 0.29941287451964627 2312 50-51 0.3141156800461964 2312 52-53 0.2991245069467894 2312 54-55 0.43042810416830335 2312 56-57 0.2608490693475467 2312 58-59 0.3035820426800413 2312 60-61 0.4681276620803416 2312 62-63 0.40363362237087586 2312 64-65 0.3604937108751578 2312 66-67 0.5045630895210564 2312 68-69 0.2193748174296566 2312 70-71 0.3124126299849941 2312 72-73 0.39858851870414114 2312 74-75 0.47202766266805085 2312 76-77 0.5096277500112159 2312 78-79 0.6583716138947686 2312 80-81 0.536397040290769 2312 82-83 0.7403204911630539 2312 84-85 0.7735901346196385 2312 86-87 0.6817264520682649 2312 88-89 0.9415512525489724 2312 90-91 1.4940567289327795 2312 92-93 2.701772079544263 2312 94-95 2.0275331247675012 2312 96-97 1.630238866870247 2312 98-99 1.461931206229071 2312 100-101 1.4452441054158154 2313 1 -0.3064156482689526 2313 2 -1.0252991503017164 2313 3 -0.4300723677387879 2313 4 -0.2818593149612454 2313 5 -0.8361556410638116 2313 6 -0.3510806995913356 2313 7 -1.1373147046360685 2313 8 -1.1338974502476802 2313 9 -1.2636088874477025 2313 10-11 -2.137486414662284 2313 12-13 -0.6473224888552238 2313 14-15 -0.20108071487629076 2313 16-17 -1.5414057129405236 2313 18-19 -1.1159267987045212 2313 20-21 -1.5481206649514192 2313 22-23 -1.1973207595694078 2313 24-25 -0.31045205698919887 2313 26-27 -0.9600293600339924 2313 28-29 -2.5993112214394642 2313 30-31 -0.7549013768318176 2313 32-33 -0.422270860720964 2313 34-35 -0.18519250764330764 2313 36-37 -0.1149275728523449 2313 38-39 -0.49343350340558345 2313 40-41 -0.3848502836139005 2313 42-43 0.024744788624900593 2313 44-45 -0.5435496622468463 2313 46-47 -0.7441092656976664 2313 48-49 -0.20791559013025562 2313 50-51 -0.27838263105268624 2313 52-53 -0.055830333203545024 2313 54-55 0.04208747346305941 2313 56-57 -0.3721752916573813 2313 58-59 -0.16081048840406353 2313 60-61 -0.36890315553623765 2313 62-63 -0.2544695461677904 2313 64-65 -0.2337206682295161 2313 66-67 -0.31040617263565906 2313 68-69 -0.35219823653383386 2313 70-71 -0.39936084809603756 2313 72-73 -0.3355139830679903 2313 74-75 -0.2164435732495278 2313 76-77 -0.0796654963001373 2313 78-79 -0.18734134106818345 2313 80-81 -0.08019602452063168 2313 82-83 -0.18242566990425146 2313 84-85 -0.04697008903452726 2313 86-87 0.005157964654699754 2313 88-89 0.2895959667360266 2313 90-91 0.9269976577514463 2313 92-93 2.260659609565245 2313 94-95 1.2989485636635152 2313 96-97 0.7824174158317341 2313 98-99 0.7614776055484214 2313 100-101 0.6576861951871518 2314 1 -0.7086917840360059 2314 2 -0.7812043570041602 2314 3 -1.8575548825904171 2314 4 -0.6046862074451269 2314 5 -0.41809654422386444 2314 6 -3.0953343357202243 2314 7 -4.135790753997597 2314 8 -1.7564022730533928 2314 9 -2.1286622053157203 2314 10-11 -1.3107062128763118 2314 12-13 -2.40985577900134 2314 14-15 -3.131409886037737 2314 16-17 -2.4075119135397074 2314 18-19 -2.8179424081316498 2314 20-21 -2.393192238668412 2314 22-23 -3.507679150505126 2314 24-25 -2.13320298707675 2314 26-27 -2.228279748415794 2314 28-29 -1.4575205197808927 2314 30-31 -1.6063084553689606 2314 32-33 -2.4426705826624513 2314 34-35 -1.0217755119034209 2314 36-37 -0.697229263387797 2314 38-39 -0.8890131720828407 2314 40-41 -0.9938863264398208 2314 42-43 -0.9130625102634369 2314 44-45 -1.5256907260297652 2314 46-47 -1.7953928416986997 2314 48-49 -2.0389867636892376 2314 50-51 -1.3391903252797341 2314 52-53 -0.8133794096920255 2314 54-55 -0.6964075561982241 2314 56-57 -0.6245523180791608 2314 58-59 -0.9013691534495756 2314 60-61 -0.6489676495345691 2314 62-63 -0.714501024798281 2314 64-65 -0.8933158945651982 2314 66-67 -0.7303664281149373 2314 68-69 -0.682351876067333 2314 70-71 -0.7472743948438669 2314 72-73 -0.5303036428233021 2314 74-75 -0.5017204401522157 2314 76-77 -0.5842674688894682 2314 78-79 -0.5081144311104175 2314 80-81 -0.5727809753147248 2314 82-83 -0.5027561609342008 2314 84-85 -0.5828006516545123 2314 86-87 -0.4380599983358131 2314 88-89 -0.2118472021982818 2314 90-91 0.6054860670829321 2314 92-93 1.4197278990016784 2314 94-95 0.7127624721548109 2314 96-97 0.6263925581528902 2314 98-99 0.649752311224745 2314 100-101 0.7374909012878827 2315 1 -7.016532888151822 2315 2 -3.0040834372352165 2315 3 -1.30457140034542 2315 4 -0.8492401984707598 2315 5 -1.2900914078887524 2315 6 -1.536106811657561 2315 7 -3.146179232024572 2315 8 -2.7806008515376526 2315 9 -3.3238489654933936 2315 10-11 -2.101718990015925 2315 12-13 -2.729101750421222 2315 14-15 -4.579307806283243 2315 16-17 -4.178490808976573 2315 18-19 -3.076478376480189 2315 20-21 -4.305393522219113 2315 22-23 -5.601618268895585 2315 24-25 -3.528644142012027 2315 26-27 -4.429094949631477 2315 28-29 -2.801837858052373 2315 30-31 -2.595545243670017 2315 32-33 -1.7009749547085136 2315 34-35 -2.576598746812607 2315 36-37 -3.1863278780629685 2315 38-39 -2.0051502485089188 2315 40-41 -3.4300484695780398 2315 42-43 -7.430147078432615 2315 44-45 -4.0451685332479315 2315 46-47 -2.795664688747099 2315 48-49 -5.555681745583932 2315 50-51 -6.630009052877941 2315 52-53 -6.920873642768335 2315 54-55 -4.42361028889167 2315 56-57 -3.8184958439649286 2315 58-59 -4.107337763507495 2315 60-61 -3.044598431064607 2315 62-63 -3.2879702632429684 2315 64-65 -2.000155763307596 2315 66-67 -2.6732396491971073 2315 68-69 -2.1192848287156494 2315 70-71 -1.7816472571265578 2315 72-73 -2.17669417649682 2315 74-75 -2.785131968220796 2315 76-77 -2.723718476527491 2315 78-79 -1.7634996390540003 2315 80-81 -1.5535135381179614 2315 82-83 -2.008897644469304 2315 84-85 -1.935661886744402 2315 86-87 -2.4393729761226943 2315 88-89 -1.8232687801287284 2315 90-91 -0.5971704898736689 2315 92-93 0.17669814423649655 2315 94-95 -0.05584632261772171 2315 96-97 -0.1897287270016612 2315 98-99 -0.38446570929688306 2315 100-101 -0.3743200311469135 2316 1 -4.585300437737786 2316 2 -3.2088390396890283 2316 3 -4.744817854658372 2316 4 -4.586456945917682 2316 5 -4.017373077625663 2316 6 -4.701980579833119 2316 7 -3.2735914617385937 2316 8 -1.7985930894787394 2316 9 -2.024446322100978 2316 10-11 -3.162317598568503 2316 12-13 -4.109670669992699 2316 14-15 -3.2303639113035416 2316 16-17 -3.1731968701787956 2316 18-19 -3.2738340898205607 2316 20-21 -5.8056483824484 2316 22-23 -6.778848966586644 2316 24-25 -4.571085077036081 2316 26-27 -4.282460893150869 2316 28-29 -4.876887488728599 2316 30-31 -6.64872160694868 2316 32-33 -4.814884063502536 2316 34-35 -6.462694109250677 2316 36-37 -9.718905275090492 2316 38-39 -6.421551622071419 2316 40-41 -5.662928121895032 2316 42-43 -4.770770010487773 2316 44-45 -3.6639736411834747 2316 46-47 -5.908447047053755 2316 48-49 -3.4022693012507688 2316 50-51 -4.5328760068957905 2316 52-53 -5.5664301323145935 2316 54-55 -7.213207520304756 2316 56-57 -5.22430039746769 2316 58-59 -5.969556746570472 2316 60-61 -6.513210381773742 2316 62-63 -6.661407567626561 2316 64-65 -5.408844261510161 2316 66-67 -6.944214198944032 2316 68-69 -5.53534191740701 2316 70-71 -6.478242861011164 2316 72-73 -8.140007717176086 2316 74-75 -8.11511336789529 2316 76-77 -7.475980919966407 2316 78-79 -6.436187564938859 2316 80-81 -6.379788286387594 2316 82-83 -5.453150322737329 2316 84-85 -6.828638475373165 2316 86-87 -5.433157963513821 2316 88-89 -5.458344701649661 2316 90-91 -5.935995986627557 2316 92-93 -4.521592056611251 2316 94-95 -4.495283483921893 2316 96-97 -5.388479464754958 2316 98-99 -6.205397335215718 2316 100-101 -5.316095646835574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27687.0 3 11610.0 4 12227.0 5 13643.0 6 15250.0 7 17821.0 8 21457.0 9 25580.0 10 30806.0 11 35542.0 12 40386.0 13 44518.0 14 47765.0 15 52235.0 16 58091.0 17 62679.0 18 61195.0 19 61756.0 20 65630.0 21 71959.0 22 77917.0 23 86651.0 24 96682.0 25 108207.0 26 121310.0 27 136658.0 28 153728.0 29 173727.0 30 197024.0 31 225003.0 32 258930.0 33 298173.0 34 348495.0 35 413125.0 36 467586.0 37 395078.0 38 118568.0 39 1957.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.337569009404284 19.02465361108229 13.682828263772572 23.95494911574086 2 15.317073148818444 20.26574618110874 40.55267317334596 23.86450749672686 3 19.310221372518146 24.643883826426478 29.48665051460278 26.559244286452593 4 12.803687203163411 16.726237069464116 37.76739578923016 32.70267993814232 5 14.021035854927213 37.0431886598913 34.57981184119769 14.355963643983799 6 31.636088686360626 36.45820912046397 17.93965067916509 13.966051514010314 7 28.01580872138869 32.28064635453643 21.833859295012957 17.869685629061923 8 25.435420186571918 35.40792421956079 20.349283898835143 18.807371695032153 9 26.30011435203359 16.671412620404247 20.555317010246767 36.4731560173154 10-11 24.80138436902733 26.269132209038826 28.123566838926735 20.805916583007118 12-13 25.89094465708323 23.712622658766495 28.089490578358156 22.306942105792118 14-15 22.882308761394988 25.167235798433023 26.093298978797037 25.857156461374952 16-17 22.628214608606324 27.98790478754041 26.659113615918052 22.72476698793522 18-19 22.53223327896548 27.175260635533398 28.292436909108957 22.000069176392163 20-21 23.33395478385876 26.40993272610878 28.155078111838634 22.101034378193827 22-23 22.92975924022928 26.308006856482795 28.0440412543621 22.718192648925818 24-25 22.88344211673651 26.460363543056232 28.17732806930861 22.47886627089865 26-27 22.8241982522356 26.53791110898395 28.204659791284815 22.433230847495636 28-29 22.59887164336596 26.921729583589304 28.1187659621703 22.36063281087443 30-31 22.869134029803625 26.52458627765897 28.029002555447136 22.577277137090274 32-33 22.755015487145723 26.552966937162747 28.108456082679 22.583561493012528 34-35 23.0685008330771 26.63568405510318 27.939960030723476 22.355855081096244 36-37 23.06394870316498 26.435331038903815 28.027470689175953 22.473249568755254 38-39 22.786893662129025 26.809095471081267 27.990993097096855 22.413017769692857 40-41 22.91631538441577 26.6715427732315 27.981115515533688 22.43102632681904 42-43 22.807905778782704 26.707593307708965 27.70321853294067 22.78128238056766 44-45 22.859829674641226 26.682029751336838 27.73807707886326 22.720063495158673 46-47 23.085704193855744 26.81378567778137 27.51894626873351 22.581563859629377 48-49 23.071719716952778 26.79513347547495 27.521123199703357 22.612023607868913 50-51 22.740158135102575 26.946493428268337 27.549692540050703 22.763655896578385 52-53 22.947388603092982 27.10247677051296 27.180505909333352 22.7696287170607 54-55 22.93262850988233 26.81940288846054 27.251822369745966 22.996146231911162 56-57 22.836691483067767 27.05537481094468 27.22011218274602 22.88782152324153 58-59 23.079529332678327 26.94340459458175 27.245876302383635 22.731189770356288 60-61 22.967737224637435 26.966479066266754 27.42087644210787 22.644907266987946 62-63 22.7865113256539 26.88860593554243 27.533889342275756 22.790993396527913 64-65 22.695847410415844 27.02574084390612 27.369970438126728 22.908441307551307 66-67 22.812007635863576 27.010946280482067 27.099553571127682 23.077492512526675 68-69 22.652008173960866 26.917580575879622 27.397832228914037 23.032579021245468 70-71 22.837198507328885 26.72148886057446 27.27006279526909 23.171249836827563 72-73 22.776676871663685 26.738983744108342 27.294795563888357 23.189543820339615 74-75 22.66552140074279 27.101816021188398 27.042938144289348 23.18972443377947 76-77 22.729417825781894 26.84281254527172 27.08505592985484 23.342713699091547 78-79 22.707413127430485 26.93682949202274 27.110510571124617 23.24524680942216 80-81 22.817650360547013 26.834132188658284 27.062313412789525 23.285904038005178 82-83 22.78501755518174 26.892286071405568 27.126793492865815 23.195902880546875 84-85 22.71884520987777 26.87497092506499 26.99003462923081 23.416149235826435 86-87 22.611549245097695 26.762402279477854 27.245679991900424 23.380368483524023 88-89 22.67281193003499 26.722634598845787 27.04069109037215 23.563862380747082 90-91 22.414559794616608 26.654439536168482 27.351124993123925 23.579875676090982 92-93 22.224210036519505 26.699397921631114 27.62677345215098 23.449618589698396 94-95 22.644653809549283 26.64381627919836 27.087329734755084 23.624200176497272 96-97 22.52842389518083 26.50054156810607 27.17242098703429 23.798613549678812 98-99 22.517664237384324 26.577493297213383 26.904830323487587 24.0000121419147 100-101 22.618027690904526 26.430788929034975 27.004388485779025 23.946794894281474 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3953.0 1 2317.5 2 701.5 3 716.0 4 658.0 5 650.0 6 860.0 7 1497.5 8 2436.5 9 2897.5 10 2729.0 11 2492.5 12 2542.5 13 2731.0 14 2979.5 15 3315.5 16 3647.5 17 3998.5 18 4427.0 19 4974.5 20 5654.0 21 6730.0 22 8278.0 23 10248.5 24 12940.0 25 16530.0 26 21718.0 27 28413.5 28 34850.5 29 41444.0 30 48508.5 31 56520.5 32 65265.5 33 73800.5 34 83027.0 35 92129.0 36 101500.5 37 109939.0 38 116088.5 39 121156.5 40 125577.0 41 130445.0 42 134496.5 43 139127.5 44 146299.5 45 152767.0 46 158517.0 47 166152.0 48 176162.5 49 178527.0 50 171571.5 51 167588.0 52 167499.5 53 165934.0 54 161345.5 55 153175.0 56 141653.0 57 127783.5 58 114956.5 59 102273.5 60 87137.0 61 72018.0 62 57604.0 63 44631.5 64 33303.5 65 23773.0 66 16423.0 67 11526.5 68 8116.0 69 5648.0 70 4040.0 71 2629.0 72 1594.0 73 974.0 74 660.5 75 430.0 76 268.0 77 191.5 78 138.0 79 97.5 80 65.0 81 46.5 82 41.5 83 26.5 84 21.0 85 22.5 86 17.0 87 12.5 88 10.0 89 7.0 90 6.5 91 5.0 92 3.5 93 4.5 94 5.0 95 5.0 96 2.5 97 2.0 98 2.5 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08979827027259901 2 0.10126426630190886 3 0.09843703440427082 4 0.10099500612118144 5 0.09767413055887643 6 0.08111462944413929 7 0.09372498124154074 8 0.08982070862099296 9 0.08391942299338338 10-11 0.09168309153769104 12-13 0.0899441195371597 14-15 0.09558736415823882 16-17 0.09313036500910099 18-19 0.09581174764217835 20-21 0.10559486754194175 22-23 0.11449167268014404 24-25 0.1361783364028994 26-27 0.1410250196559932 28-29 0.14113721139796295 30-31 0.14267423826294873 32-33 0.13891581490696164 34-35 0.13434961100879225 36-37 0.13837729454550676 38-39 0.13120824223363886 40-41 0.1279098050197278 42-43 0.12753957227122759 44-45 0.11876617804919204 46-47 0.13708708951285448 48-49 0.15411779594386463 50-51 0.16371018988227945 52-53 0.17225920062037545 54-55 0.16837736634822162 56-57 0.13999285562987135 58-59 0.13073703691736585 60-61 0.12803321593589453 62-63 0.1253967099996051 64-65 0.11165322160830901 66-67 0.12464502532840767 68-69 0.12224412205025471 70-71 0.13110726966586606 72-73 0.1579323151708366 74-75 0.17090168054254132 76-77 0.1755688570084835 78-79 0.17270796758825452 80-81 0.17948434880322825 82-83 0.17882241752560665 84-85 0.15587920629278992 86-87 0.15797719186762452 88-89 0.17766684258331808 90-91 0.2669153733202652 92-93 0.5406183470297011 94-95 0.1764102950732567 96-97 0.18987330410962838 98-99 0.20759959934085107 100-101 0.21460036403976435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4456656.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.35784252233018 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7080539986237 39.32902737885972 2 13.413513519432637 14.31432284082067 3 5.145597381785263 8.236739243418377 4 2.5318561152639125 5.40377519549802 5 1.422292801742057 3.7945237667998235 6 0.9010150261323682 2.8845730724774463 7 0.6090757014940424 2.274927575514783 8 0.4303836156732877 1.8371472951429089 9 0.31444357739198464 1.510022779617569 >10 1.4091477413866011 12.90505900794938 >50 0.07896999330592096 2.9019053310514034 >100 0.033692862649028626 3.0480963462650568 >500 0.0012767383143612507 0.4597007218684189 >1k 5.106951037262473E-4 0.44725658616565833 >5k 1.2767377593151863E-4 0.4119456605213955 >10k+ 4.2557925310506215E-5 0.24097719802949388 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10612 0.23811575315662686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7093 0.15915520515830703 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5962 0.13377743312474644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5086 0.11412143993164382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2438348393952777E-5 2 0.0 0.0 0.0 0.0 4.4876696787905554E-5 3 0.0 0.0 0.0 0.0 4.4876696787905554E-5 4 0.0 0.0 0.0 0.0 4.4876696787905554E-5 5 0.0 0.0 0.0 0.0 4.4876696787905554E-5 6 0.0 0.0 0.0 0.0 4.4876696787905554E-5 7 0.0 0.0 0.0 0.0 4.4876696787905554E-5 8 0.0 0.0 0.0 0.0 4.4876696787905554E-5 9 0.0 0.0 0.0 2.2438348393952777E-5 6.731504518185832E-5 10-11 2.2438348393952777E-5 0.0 0.0 2.2438348393952777E-5 6.731504518185832E-5 12-13 2.2438348393952777E-5 0.0 0.0 3.365752259092917E-5 6.731504518185832E-5 14-15 2.2438348393952777E-5 0.0 0.0 7.853421937883471E-5 8.975339357581111E-5 16-17 2.2438348393952777E-5 0.0 0.0 1.6828761295464582E-4 1.1219174196976388E-4 18-19 2.2438348393952777E-5 0.0 0.0 2.3560265813650416E-4 1.3463009036371665E-4 20-21 2.2438348393952777E-5 0.0 0.0 3.5901357430324443E-4 1.3463009036371665E-4 22-23 2.2438348393952777E-5 0.0 0.0 5.273011872578902E-4 1.3463009036371665E-4 24-25 2.2438348393952777E-5 0.0 0.0 8.97533935758111E-4 1.3463009036371665E-4 26-27 2.2438348393952777E-5 0.0 0.0 0.0013238625552432137 1.3463009036371665E-4 28-29 2.2438348393952777E-5 0.0 0.0 0.002580410065304569 1.3463009036371665E-4 30-31 2.2438348393952777E-5 0.0 0.0 0.006787600389170714 1.4584926456069305E-4 32-33 2.2438348393952777E-5 0.0 0.0 0.017602884315055953 1.5706843875766942E-4 34-35 4.4876696787905554E-5 0.0 0.0 0.03194098893879178 1.7950678715162222E-4 36-37 4.4876696787905554E-5 0.0 0.0 0.055591008146018 1.907259613485986E-4 38-39 4.4876696787905554E-5 0.0 0.0 0.08832855845279511 2.01945135545575E-4 40-41 4.4876696787905554E-5 3.365752259092917E-5 0.0 0.1251498881672716 2.01945135545575E-4 42-43 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.1660213397668566 2.1316430974255139E-4 44-45 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.21677688383397775 2.2438348393952776E-4 46-47 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.2791442731949695 2.2438348393952776E-4 48-49 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.34384525078893236 2.4682183233348055E-4 50-51 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.4109471316610481 2.4682183233348055E-4 52-53 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.4820318193730905 2.580410065304569E-4 54-55 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.563011818726866 2.916985291213861E-4 56-57 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.6579484707816803 3.1413687751533884E-4 58-59 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.7619389964134544 3.1413687751533884E-4 60-61 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.8737156289379302 3.1413687751533884E-4 62-63 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.9947032034781236 3.814519226971972E-4 64-65 4.4876696787905554E-5 4.4876696787905554E-5 0.0 1.1212330500716232 3.814519226971972E-4 66-67 4.4876696787905554E-5 4.4876696787905554E-5 0.0 1.2664652600514825 3.814519226971972E-4 68-69 4.4876696787905554E-5 4.4876696787905554E-5 0.0 1.4314207782696262 3.814519226971972E-4 70-71 4.4876696787905554E-5 4.4876696787905554E-5 0.0 1.61181388018281 4.0389027109115E-4 72-73 4.4876696787905554E-5 4.4876696787905554E-5 0.0 1.8032017728090297 4.0389027109115E-4 74-75 4.4876696787905554E-5 4.4876696787905554E-5 0.0 2.0057303054128477 4.263286194851027E-4 76-77 4.4876696787905554E-5 4.4876696787905554E-5 0.0 2.224616393995857 4.487669678790555E-4 78-79 4.4876696787905554E-5 4.4876696787905554E-5 0.0 2.4763073479308253 4.487669678790555E-4 80-81 4.4876696787905554E-5 4.4876696787905554E-5 0.0 2.751087362363171 4.599861420760319E-4 82-83 4.4876696787905554E-5 6.731504518185832E-5 0.0 3.0258225000987284 4.936436646669611E-4 84-85 4.4876696787905554E-5 6.731504518185832E-5 0.0 3.305853088055259 4.936436646669611E-4 86-87 6.731504518185832E-5 6.731504518185832E-5 0.0 3.6095336952190165 4.936436646669611E-4 88-89 8.975339357581111E-5 6.731504518185832E-5 0.0 3.9344409799634525 4.936436646669611E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19730 0.0 26.169916 1 GTACATG 11935 0.0 24.573477 1 TACATGG 11895 0.0 23.658958 2 CAACGCA 22300 0.0 23.239046 5 ATCAACG 22405 0.0 23.088293 3 TCAACGC 22460 0.0 23.052626 4 TATCAAC 22440 0.0 22.92513 2 AACGCAG 22910 0.0 22.6983 6 ACATGGG 12220 0.0 21.864899 3 GAGTACT 9820 0.0 20.87441 12-13 AGAGTAC 15255 0.0 20.84033 10-11 ACGCAGA 24715 0.0 20.637667 7 GGTATCA 14335 0.0 20.095161 1 CGCAGAG 24860 0.0 20.07877 8 AGTACTT 10705 0.0 19.104364 12-13 GTATAGG 1385 0.0 18.84575 1 GTACTTT 11220 0.0 17.953585 14-15 GCAGAGT 25570 0.0 17.944563 9 CATGGGG 7515 0.0 17.303223 4 CATGGGA 5985 0.0 16.096718 4 >>END_MODULE