FastQCFastQC Report
Fri 27 May 2016
SRR939677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4456656
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113980.2557522949942737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89390.20057639629354387No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64550.14483953888296516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60330.13537055586071708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA181700.027.6043741
GGTATCA123200.025.5118161
ATCAACG207200.023.7916833
TCAACGC207400.023.791424
CAACGCA207900.023.688955
AACGCAG210400.023.4424156
TATCAAC215250.023.0129872
GTACATG124300.021.689981
TACATGG119500.021.651232
CGCAGAG230650.020.79398
ACGCAGA232700.020.7719757
ACATGGG116350.020.4972323
GAGTACT105400.020.16678812-13
AGAGTAC158550.020.14658510-11
AGTACTT113300.018.6569812-13
GCAGAGT245150.018.5673839
GTACTTT114100.018.52606814-15
CATGGGG72850.017.6273384
GAGTACA96050.015.2838541
CAGAGTA232700.015.07936410-11
Produced by FastQC (version 0.11.5)