##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939677_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4456656 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.563346150117937 31.0 31.0 34.0 30.0 34.0 2 31.77388046104523 31.0 31.0 34.0 30.0 34.0 3 31.822358063983398 31.0 31.0 34.0 30.0 34.0 4 35.60578738857116 37.0 35.0 37.0 33.0 37.0 5 35.333438793570785 37.0 35.0 37.0 33.0 37.0 6 35.35292088956383 37.0 35.0 37.0 33.0 37.0 7 35.28262378788042 37.0 35.0 37.0 32.0 37.0 8 35.248335523316136 37.0 35.0 37.0 32.0 37.0 9 36.870698344229396 39.0 37.0 39.0 33.0 39.0 10-11 36.74983002951092 39.0 36.0 39.0 32.0 39.0 12-13 36.69447765320007 39.0 35.0 39.0 32.0 39.0 14-15 37.777333386287836 40.0 37.0 41.0 32.0 41.0 16-17 37.734705236392486 39.5 37.0 41.0 32.0 41.0 18-19 37.70049786207416 39.5 37.0 41.0 32.0 41.0 20-21 37.62726010264198 39.0 36.5 41.0 32.0 41.0 22-23 37.489235763316714 39.0 36.0 41.0 32.0 41.0 24-25 37.364490101995756 39.0 36.0 41.0 31.5 41.0 26-27 37.11247334324211 39.0 36.0 40.0 31.0 41.0 28-29 36.920039599197246 39.0 36.0 40.0 30.5 41.0 30-31 36.69868136557993 38.5 35.5 40.0 30.0 41.0 32-33 36.54947195834724 38.0 35.0 40.0 30.0 41.0 34-35 36.54461888016486 38.0 35.0 40.0 30.0 41.0 36-37 36.46561659683853 38.5 35.0 40.0 30.0 41.0 38-39 36.519237517995556 39.0 35.0 40.0 30.0 41.0 40-41 36.440648661238384 39.0 35.0 40.0 30.0 41.0 42-43 36.27113355843485 38.5 35.0 40.0 30.0 41.0 44-45 36.086546392631604 38.0 35.0 40.0 29.5 41.0 46-47 35.830755728061575 38.0 35.0 40.0 28.5 41.0 48-49 35.56169850219537 38.0 34.0 40.0 27.5 41.0 50-51 35.32633200767571 38.0 34.0 40.0 27.0 41.0 52-53 35.095715262744086 38.0 34.0 40.0 27.0 41.0 54-55 34.810239224207564 38.0 34.0 40.0 26.0 41.0 56-57 32.79956608721876 37.0 30.5 39.5 16.5 41.0 58-59 27.90992764530177 33.0 18.5 38.5 7.5 40.0 60-61 28.68173087624443 33.5 22.0 38.0 9.5 40.0 62-63 31.61209536926341 35.0 30.0 38.5 16.0 40.0 64-65 31.80630712803501 35.5 31.0 39.0 12.5 40.0 66-67 25.119917938472256 27.5 18.0 35.5 2.0 39.0 68-69 17.56605468315257 13.0 7.5 28.0 2.0 35.0 70-71 16.42188526554439 12.5 7.5 27.0 2.0 33.5 72-73 17.58438109201159 14.5 7.0 28.5 2.0 34.0 74-75 18.637010574744828 17.5 9.0 30.0 2.0 34.5 76-77 19.907118364082848 22.0 8.0 30.0 2.0 34.5 78-79 23.1023471409954 27.5 10.5 33.5 2.0 35.5 80-81 26.660626936429466 31.5 24.0 34.5 2.0 36.0 82-83 27.89692798815973 33.5 27.5 35.0 2.0 36.0 84-85 27.941007001662232 34.0 28.0 35.0 2.0 36.0 86-87 27.702794202648803 34.0 27.5 35.0 2.0 36.0 88-89 27.289497663719164 33.5 26.5 35.0 2.0 35.0 90-91 26.08242469241512 33.0 23.0 35.0 2.0 35.0 92-93 23.67340086378666 31.5 4.5 34.5 2.0 35.0 94-95 16.200470150713898 8.5 2.0 32.0 2.0 35.0 96-97 13.594053927428995 2.0 2.0 30.0 2.0 34.0 98-99 13.278205452698167 2.0 2.0 30.0 2.0 34.0 100-101 12.990609326813647 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38114197112128423 1101 2 0.4304476367084824 1101 3 0.40687291624953303 1101 4 0.2682070361989872 1101 5 0.47432183786784066 1101 6 0.47377708957785813 1101 7 0.48415369627340255 1101 8 0.4202463960409375 1101 9 0.4971137348173116 1101 10-11 0.5711825840110691 1101 12-13 0.4743215047316056 1101 14-15 0.6506004493754034 1101 16-17 0.641537672344711 1101 18-19 0.5561074575087233 1101 20-21 0.505700309218625 1101 22-23 0.7170094343484053 1101 24-25 0.6077648292582936 1101 26-27 0.6575313674829673 1101 28-29 0.6406447394051042 1101 30-31 0.5389748445941933 1101 32-33 0.6511352540613302 1101 34-35 0.5529022433251214 1101 36-37 0.3540486276157395 1101 38-39 0.6110963056158525 1101 40-41 0.6136926326484371 1101 42-43 0.614363229633959 1101 44-45 0.44680800478066374 1101 46-47 0.5443885341202446 1101 48-49 0.46971084517562645 1101 50-51 0.28370558329761053 1101 52-53 0.16412988947714524 1101 54-55 0.20025975403496687 1101 56-57 -2.3715766377150764 1101 58-59 -10.069040793502225 1101 60-61 -10.03242390097056 1101 62-63 -3.675966853144242 1101 64-65 -5.0465288598360765 1101 66-67 -9.99859308416426 1101 68-69 -4.940500228567817 1101 70-71 -4.626713018381791 1101 72-73 -4.607992042508432 1101 74-75 -6.576952614778451 1101 76-77 -7.952057381533841 1101 78-79 -11.27947489427058 1101 80-81 -7.945676567523403 1101 82-83 -4.187730668088303 1101 84-85 -3.293797623982492 1101 86-87 -3.1282720181940213 1101 88-89 -3.2960062855820063 1101 90-91 -10.133853532764224 1101 92-93 -17.270007024873504 1101 94-95 -11.176873007352285 1101 96-97 -9.386234757902242 1101 98-99 -9.571707809920236 1101 100-101 -9.428885611717735 1102 1 0.3791681514788401 1102 2 0.42741524900997163 1102 3 0.44926970398679344 1102 4 0.34268182585578444 1102 5 0.3669220202101542 1102 6 0.31651182956520074 1102 7 0.4275492689315783 1102 8 0.461482384737387 1102 9 0.6038555410789712 1102 10-11 0.49832691646150806 1102 12-13 0.42750668645228274 1102 14-15 0.5159186897109009 1102 16-17 0.598726167936924 1102 18-19 0.5849615361334699 1102 20-21 0.6059722243289372 1102 22-23 0.6434179289816058 1102 24-25 0.6068297858390892 1102 26-27 0.5570672731395376 1102 28-29 0.561772622857923 1102 30-31 0.5272437586404664 1102 32-33 0.5785394548858918 1102 34-35 0.4165162405813021 1102 36-37 0.4532470101134578 1102 38-39 0.25293214239254525 1102 40-41 0.30564568105732803 1102 42-43 0.46051149760185695 1102 44-45 0.46492230545797497 1102 46-47 0.5187667460692182 1102 48-49 0.4281642480675103 1102 50-51 0.38969857173241707 1102 52-53 0.20657956270807887 1102 54-55 0.17964234968717818 1102 56-57 -2.9528247396568545 1102 58-59 -10.496578280789059 1102 60-61 -10.863717298854947 1102 62-63 -4.5186280576226245 1102 64-65 -5.446879931550413 1102 66-67 -7.0911518699304885 1102 68-69 -4.553613020453103 1102 70-71 -4.372424171117135 1102 72-73 -4.615430338921861 1102 74-75 -5.349443136421437 1102 76-77 -7.520444546685798 1102 78-79 -9.840414108456507 1102 80-81 -5.806733610160162 1102 82-83 -3.354841801750542 1102 84-85 -2.93346236642531 1102 86-87 -2.8481578327277077 1102 88-89 -2.9677341190242394 1102 90-91 -8.92336814155049 1102 92-93 -13.63647040242004 1102 94-95 -10.432204170842802 1102 96-97 -9.250748452187832 1102 98-99 -9.340472425169688 1102 100-101 -9.362867941955567 1103 1 0.24380370583463318 1103 2 0.22012620806775018 1103 3 0.21772876560783772 1103 4 0.25310077302362544 1103 5 0.23671799549801875 1103 6 0.25329377583305046 1103 7 0.19636042627450223 1103 8 0.23695147433144115 1103 9 0.35610456130651613 1103 10-11 0.3743308278678512 1103 12-13 0.39847319593190633 1103 14-15 0.5012796009517899 1103 16-17 0.4083707606327991 1103 18-19 0.45579001179654455 1103 20-21 0.3204205129349873 1103 22-23 0.4579034376718809 1103 24-25 0.5897749452176342 1103 26-27 0.5237186731474281 1103 28-29 0.49707261686480564 1103 30-31 0.520912150420358 1103 32-33 0.4356979534098926 1103 34-35 0.47155788123083653 1103 36-37 0.34490794404624125 1103 38-39 0.30850256691536515 1103 40-41 0.4056921533386415 1103 42-43 0.3425327626723771 1103 44-45 0.23510205163659492 1103 46-47 0.2741463064099321 1103 48-49 0.10612113500604181 1103 50-51 0.269782798100195 1103 52-53 0.25162806214897415 1103 54-55 0.2692210974736966 1103 56-57 -2.8709821092207264 1103 58-59 -10.48361611273484 1103 60-61 -10.670985869711284 1103 62-63 -4.686058313344393 1103 64-65 -5.887462449879372 1103 66-67 -6.992991757362304 1103 68-69 -4.358165511208588 1103 70-71 -4.256730408724312 1103 72-73 -4.566627720492459 1103 74-75 -5.243851268571241 1103 76-77 -6.912903762744897 1103 78-79 -9.564879741250444 1103 80-81 -5.513203175361962 1103 82-83 -3.2043616991606463 1103 84-85 -2.761614170584533 1103 86-87 -2.565197056664939 1103 88-89 -2.6957250630121443 1103 90-91 -5.541349693757979 1103 92-93 -10.65387122398953 1103 94-95 -8.699353550350125 1103 96-97 -8.194766545003311 1103 98-99 -8.426900623766274 1103 100-101 -8.486644827111535 1104 1 0.14882105608101526 1104 2 0.1740147077910592 1104 3 0.1557376121776386 1104 4 0.12587045816918163 1104 5 0.17982445535744773 1104 6 0.17029088745743337 1104 7 0.15949445339136048 1104 8 0.18064191832347376 1104 9 0.12181856760835785 1104 10-11 0.18165595515139898 1104 12-13 0.2024744703705963 1104 14-15 0.3403672409146594 1104 16-17 0.29854166404894755 1104 18-19 0.2947978460957543 1104 20-21 0.17633837755893467 1104 22-23 0.28524297665590126 1104 24-25 0.3541383143378525 1104 26-27 0.3807469799452008 1104 28-29 0.27237433649885645 1104 30-31 0.3407872310983464 1104 32-33 0.20590922693948244 1104 34-35 0.29595724753849595 1104 36-37 0.23813942413409706 1104 38-39 0.233210626716847 1104 40-41 0.09275563875587522 1104 42-43 0.2678812246132978 1104 44-45 0.2504556003928755 1104 46-47 0.18106204124822511 1104 48-49 0.2524192744424951 1104 50-51 0.18757150646403176 1104 52-53 0.14378561970053028 1104 54-55 0.050818623436825305 1104 56-57 -3.1476518181688746 1104 58-59 -10.44098050864864 1104 60-61 -10.610282475558726 1104 62-63 -4.285855755489173 1104 64-65 -5.5527834303804795 1104 66-67 -6.770193959876025 1104 68-69 -4.138036264227011 1104 70-71 -3.9645747702808904 1104 72-73 -4.261658475261182 1104 74-75 -4.946024099771581 1104 76-77 -6.776414929973811 1104 78-79 -9.248657190155015 1104 80-81 -4.929407582162337 1104 82-83 -2.7100187301126972 1104 84-85 -2.1651139636792145 1104 86-87 -2.152319213980128 1104 88-89 -2.113026473514182 1104 90-91 -4.661548316262714 1104 92-93 -6.851455123436185 1104 94-95 -8.036115368001091 1104 96-97 -7.978885010463703 1104 98-99 -8.29812064088453 1104 100-101 -8.343617116699011 1105 1 0.3559018936495626 1105 2 0.36365089162026365 1105 3 0.3635298411515393 1105 4 0.26062982697403925 1105 5 0.31230891877605416 1105 6 0.3365184915129902 1105 7 0.32116926429200987 1105 8 0.3549655722414755 1105 9 0.45632313843560723 1105 10-11 0.4705831754209413 1105 12-13 0.45158040804233934 1105 14-15 0.5698313944022217 1105 16-17 0.5227472482431352 1105 18-19 0.5673564468812131 1105 20-21 0.47861005824420033 1105 22-23 0.5592483824104235 1105 24-25 0.6294714860633306 1105 26-27 0.647819954276109 1105 28-29 0.6686611958330246 1105 30-31 0.648467415692906 1105 32-33 0.5880416527338426 1105 34-35 0.6209086649949569 1105 36-37 0.5525962805706897 1105 38-39 0.5021469842605129 1105 40-41 0.5124709060467723 1105 42-43 0.5405946529858454 1105 44-45 0.5440656503505821 1105 46-47 0.6587732295719633 1105 48-49 0.5771271850347759 1105 50-51 0.6279955518476967 1105 52-53 0.7175093207961041 1105 54-55 0.6483862156392703 1105 56-57 1.8288938644271653 1105 58-59 -8.522594079122381 1105 60-61 -9.746914617420963 1105 62-63 -3.378371751077861 1105 64-65 -4.6533051422642515 1105 66-67 -6.484007525745714 1105 68-69 -3.789972384950868 1105 70-71 -3.841747983416756 1105 72-73 -2.088094047366475 1105 74-75 -4.2479019661789295 1105 76-77 -6.2825847568940425 1105 78-79 -8.949973408714062 1105 80-81 -4.38041356305067 1105 82-83 -1.726698938659517 1105 84-85 -1.2220302855778158 1105 86-87 -1.02338490402839 1105 88-89 -1.0076641685724788 1105 90-91 -3.9467459275235193 1105 92-93 -6.341424928678226 1105 94-95 -7.905305069610935 1105 96-97 -8.210388647525658 1105 98-99 -8.673064220267207 1105 100-101 -8.649739146281075 1106 1 0.17396097666673782 1106 2 0.16978602079541005 1106 3 0.12526272311282227 1106 4 0.16332284304416333 1106 5 0.03449915801208192 1106 6 0.1676317393049942 1106 7 0.11938120223911852 1106 8 0.06316678802120634 1106 9 0.1057099383391602 1106 10-11 0.17471530357356357 1106 12-13 0.17714953319509874 1106 14-15 0.2623877913420287 1106 16-17 0.21417314460101267 1106 18-19 0.24145929729906612 1106 20-21 0.2709167583179237 1106 22-23 0.3267580267611905 1106 24-25 0.36448899825015957 1106 26-27 0.40631697262789856 1106 28-29 0.4683076050397972 1106 30-31 0.4080274141944571 1106 32-33 0.2833086184814846 1106 34-35 0.43804804763550464 1106 36-37 0.30309380123844676 1106 38-39 0.4067419974807436 1106 40-41 0.38299700156738936 1106 42-43 0.3734018332978408 1106 44-45 0.2777539948306824 1106 46-47 0.23894022581644236 1106 48-49 0.2777064446890094 1106 50-51 0.37977090795843793 1106 52-53 0.43723290798094183 1106 54-55 0.21143752254261727 1106 56-57 1.5048028381307503 1106 58-59 -9.186884260441452 1106 60-61 -10.538742398738183 1106 62-63 -4.243339865857184 1106 64-65 -5.902446203839929 1106 66-67 -7.450050734445384 1106 68-69 -4.383107584542939 1106 70-71 -4.316621982823811 1106 72-73 -4.831279311202726 1106 74-75 -5.432277287609015 1106 76-77 -7.05761488362262 1106 78-79 -9.677409258188685 1106 80-81 -5.438091653178926 1106 82-83 -2.920268528280758 1106 84-85 -2.4430976194759424 1106 86-87 -2.2719056015189594 1106 88-89 -2.347657608341354 1106 90-91 -4.814528273318505 1106 92-93 -7.170953956606711 1106 94-95 -8.038603308196603 1106 96-97 -7.9772471284330795 1106 98-99 -7.449893615251953 1106 100-101 -7.664956475177928 1107 1 0.026752073253849318 1107 2 -0.05224278168660135 1107 3 -0.045387403146374794 1107 4 0.06931809084088769 1107 5 -0.025774241943544496 1107 6 0.010673176985349642 1107 7 0.0828210471082258 1107 8 0.14244950261372225 1107 9 0.21677276939771417 1107 10-11 0.1208412535738006 1107 12-13 0.0633200900351838 1107 14-15 0.13346310318774357 1107 16-17 0.08735231668246257 1107 18-19 0.1142405298764757 1107 20-21 0.12665323415815521 1107 22-23 0.15447887190132548 1107 24-25 0.16542746467187186 1107 26-27 0.18056437169592954 1107 28-29 0.25117711028516965 1107 30-31 0.2282869014703266 1107 32-33 0.23031050558302724 1107 34-35 0.19842840695904584 1107 36-37 0.1725099294773429 1107 38-39 0.10768662028831955 1107 40-41 0.05978925119165979 1107 42-43 0.06478565781400647 1107 44-45 0.1312463363681644 1107 46-47 0.1085123325486137 1107 48-49 -0.0641194829363414 1107 50-51 0.02024975942509144 1107 52-53 0.013118576497682 1107 54-55 0.05012541741411525 1107 56-57 1.2341798388386067 1107 58-59 -8.810886598363467 1107 60-61 -10.396250089361864 1107 62-63 -3.962208379811589 1107 64-65 -5.378813863454731 1107 66-67 -7.192006102904767 1107 68-69 -4.054510112685993 1107 70-71 -4.117334788238072 1107 72-73 -2.601331559609509 1107 74-75 -4.805883331700397 1107 76-77 -6.890656739460752 1107 78-79 -7.715266768198923 1107 80-81 -2.6529366366993123 1107 82-83 -1.5085220123907952 1107 84-85 -1.178033203430143 1107 86-87 -1.1148067964784758 1107 88-89 -0.965551896174464 1107 90-91 -0.5489276185097545 1107 92-93 -3.066778221757108 1107 94-95 -5.78840396229066 1107 96-97 -6.527798767564549 1107 98-99 -6.326596848050418 1107 100-101 -7.156871485456021 1108 1 -0.01336215500842286 1108 2 -0.0560241593059736 1108 3 -0.05183154477579066 1108 4 0.0038174949107272482 1108 5 6.964035778125321E-4 1108 6 -0.030378617848754175 1108 7 -0.0983495206827314 1108 8 -0.07437770186707127 1108 9 -0.07778269955834105 1108 10-11 -0.03077814572738191 1108 12-13 0.04320672278119986 1108 14-15 -0.004771952686510872 1108 16-17 -0.05313358306488425 1108 18-19 0.0691348500409319 1108 20-21 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96-97 14.060808910503653 2316 98-99 13.722328648976903 2316 100-101 12.459015187173774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 17.0 4 52.0 5 150.0 6 299.0 7 843.0 8 1991.0 9 3447.0 10 5616.0 11 8875.0 12 13360.0 13 18920.0 14 23324.0 15 32151.0 16 44221.0 17 59508.0 18 68885.0 19 71505.0 20 75241.0 21 75093.0 22 75499.0 23 63629.0 24 65260.0 25 77428.0 26 110062.0 27 163639.0 28 230968.0 29 310844.0 30 387966.0 31 430096.0 32 437741.0 33 404803.0 34 362667.0 35 326606.0 36 265844.0 37 171461.0 38 64410.0 39 4232.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.59752848681538 19.709038349750983 14.489773474549834 24.203659688883803 2 15.588377259957909 20.937955040612987 39.53856231120969 23.935105388219416 3 19.01263327859855 24.80870125982747 30.130129366460217 26.048536095113768 4 13.138011998233653 17.43544935934028 37.375197906232835 32.05134073619323 5 13.777918283872342 37.35566481477237 34.700301848434066 14.166115052921215 6 31.659701803325184 36.98219023411275 17.534380037409214 13.823727925152852 7 27.726092265632467 32.43998748837132 21.821819688043988 18.01210055795222 8 25.286480181825077 35.94274188499685 20.15435116213267 18.616426771045404 9 25.773629375926703 16.63868604621941 20.458859736986657 37.12882484086723 10-11 24.701289487005504 26.448586114791002 28.144375513838177 20.70574888436532 12-13 25.615169759568605 23.770019494437083 28.216144571176237 22.398666174818068 14-15 22.559905543210682 25.29658547771741 26.15319474913954 25.990314229932366 16-17 22.338946510567563 28.147730046923076 26.763261512667796 22.750061929841568 18-19 22.280316444036764 27.293881568864858 28.380662241967002 22.045139745131376 20-21 23.03769911790365 26.51122276433272 28.22697107427632 22.224107043487315 22-23 22.635141578601463 26.449482917740063 28.131691558732236 22.783683944926235 24-25 22.679366327365067 26.608692686408602 28.172331958871837 22.539609027354487 26-27 22.633611749109868 26.675610616418993 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22.7949440205497 52-53 22.880524073403695 27.061368562602343 27.230559240990942 22.827548123003016 54-55 22.82631430689516 26.791024139894244 27.317780921138386 23.06488063207221 56-57 21.95641499624516 26.9035606875686 28.315923581826585 22.824100734359657 58-59 21.41217204677201 26.459236733338265 29.70608955784084 22.422501662048884 60-61 22.649490951010907 26.793987845353595 28.00610270036709 22.55041850326841 62-63 22.769501629997702 26.864545485552622 27.606234830968518 22.75971805348116 64-65 22.319812457806936 26.960729141041927 27.8289767066216 22.890481694529534 66-67 22.096853320787925 26.518285648124206 28.666934537960913 22.71792649312695 68-69 21.997842079813463 26.009918666564346 29.572301420954382 22.419937832667806 70-71 21.958126140697285 25.975204891083653 29.484294849183467 22.5823741190356 72-73 21.17692426523653 26.217423583907255 29.88727516710436 22.718376983751853 74-75 21.78330415234389 26.46516646564292 28.957681475994946 22.79384790601824 76-77 20.230390518120284 26.914240177858094 29.39715763518033 23.45821166884129 78-79 21.225710980060864 26.94618565169708 28.59506719742243 23.233036170819624 80-81 22.363012995130152 26.91392964307872 27.391563846222667 23.33149351556846 82-83 22.729338589152494 26.913750750984907 27.170614234130024 23.18629642573257 84-85 22.673405811564454 26.901728956187593 27.04153132112618 23.383333911121778 86-87 22.54945066780714 26.84277623145786 27.255093721422547 23.35267937931246 88-89 22.60027765243453 26.83400607317019 27.03266421355408 23.5330520608412 90-91 22.02274200454786 26.764714384227332 27.63234422923631 23.580199381988496 92-93 21.27269409519862 26.998736181917714 28.064071747020115 23.66449797586355 94-95 19.995162344348085 26.886805958470834 29.348982569092925 23.769049128088156 96-97 19.845911456260286 26.924841399740757 29.1204202406463 24.10882690335266 98-99 20.43150215723759 26.962350848267576 28.399757946809768 24.20638904768507 100-101 20.9638589702344 26.73268280083918 28.09182295607886 24.211635272847555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 508.0 1 377.5 2 281.5 3 320.5 4 376.0 5 471.0 6 607.5 7 866.0 8 1213.0 9 1540.0 10 1742.5 11 1992.5 12 2350.0 13 2692.0 14 3094.5 15 3516.5 16 3924.0 17 4388.5 18 4909.0 19 5574.5 20 6415.0 21 7498.0 22 9145.0 23 11308.5 24 14211.5 25 17991.5 26 23038.5 27 29278.0 28 35961.0 29 43341.0 30 51169.5 31 59527.0 32 68950.0 33 79103.0 34 89093.0 35 98562.5 36 107762.0 37 115902.5 38 123099.5 39 129873.5 40 135580.0 41 141082.0 42 146627.0 43 153210.0 44 160126.0 45 165927.0 46 172146.0 47 177872.0 48 181668.0 49 181735.0 50 178241.5 51 174458.0 52 169842.5 53 163112.0 54 154661.5 55 143741.5 56 130741.0 57 115811.0 58 100282.0 59 84662.0 60 68992.5 61 54747.0 62 41811.0 63 30878.5 64 22194.5 65 15430.5 66 10411.0 67 7017.0 68 4677.5 69 2924.0 70 1828.0 71 1076.0 72 608.5 73 357.5 74 201.0 75 114.0 76 63.5 77 33.0 78 17.0 79 8.0 80 5.0 81 4.0 82 3.5 83 2.0 84 1.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024368046355832715 2 0.007247586531246746 3 3.1413687751533884E-4 4 0.0 5 0.13638028153844498 6 0.0 7 4.4876696787905554E-5 8 0.0014584926456069304 9 0.0 10-11 0.0 12-13 0.0 14-15 3.3657522590929164E-4 16-17 0.0 18-19 1.5706843875766942E-4 20-21 0.0 22-23 3.3657522590929164E-4 24-25 3.365752259092916E-5 26-27 2.692601807274333E-4 28-29 0.002782355200850144 30-31 0.010882598971067097 32-33 0.0026477251104864274 34-35 0.0039042726205477827 36-37 0.10162327987621213 38-39 0.10115207455993912 40-41 0.11308927590552198 42-43 0.11580431606119028 44-45 0.11163078325991506 46-47 0.10535926488380526 48-49 0.10419247076731972 50-51 0.1036763887542588 52-53 0.08284238227047365 54-55 0.07129785202178494 56-57 0.5618562437845775 58-59 1.5988557339853022 60-61 0.5281201869742694 62-63 0.0046671764659421775 64-65 0.12096513619179942 66-67 1.938628424540732 68-69 2.92299876858344 70-71 2.832740512168765 72-73 2.6824147971034784 74-75 2.0438081826373855 76-77 1.4347192154835373 78-79 0.9552678061757515 80-81 0.05408763880362316 82-83 1.907259613485986E-4 84-85 1.6828761295464582E-4 86-87 3.9267109689417355E-4 88-89 1.1219174196976388E-4 90-91 0.2528689672256508 92-93 0.3977421636312069 94-95 2.039758060752277 96-97 2.472571362923232 98-99 1.4412824323887687 100-101 0.961169091803361 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4456656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.037992219591594 #Duplication Level Percentage of deduplicated Percentage of total 1 68.49847283980826 32.220306324928075 2 15.397940237292437 14.485763861589962 3 6.223687973307543 8.78249359396818 4 3.2005662355577695 6.021928387458156 5 1.800541989115085 4.234694003752168 6 1.114326261150142 3.1449402001240188 7 0.7897790748038915 2.6004735383075186 8 0.5374257450641 2.0223542411946682 9 0.40212103407030125 1.7023469464739662 >10 1.873191453763192 15.166129587983553 >50 0.10751440586505163 3.4927634804113032 >100 0.051562774068664434 4.167683923295375 >500 0.002008983532438092 0.6518824472038599 >1k 6.696609119259644E-4 0.5676885748029331 >5k 1.434987668412576E-4 0.48209992042730926 >10k+ 4.783292228041921E-5 0.25645096807908113 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11398 0.2557522949942737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8939 0.20057639629354387 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6455 0.14483953888296516 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6033 0.13537055586071708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2438348393952777E-5 2 0.0 0.0 0.0 0.0 2.2438348393952777E-5 3 0.0 0.0 0.0 0.0 2.2438348393952777E-5 4 0.0 0.0 0.0 0.0 2.2438348393952777E-5 5 0.0 0.0 0.0 0.0 2.2438348393952777E-5 6 0.0 0.0 0.0 0.0 6.731504518185832E-5 7 2.2438348393952777E-5 0.0 0.0 0.0 6.731504518185832E-5 8 2.2438348393952777E-5 0.0 0.0 0.0 6.731504518185832E-5 9 2.2438348393952777E-5 0.0 0.0 2.2438348393952777E-5 8.975339357581111E-5 10-11 2.2438348393952777E-5 0.0 0.0 2.2438348393952777E-5 8.975339357581111E-5 12-13 2.2438348393952777E-5 0.0 0.0 3.365752259092917E-5 8.975339357581111E-5 14-15 2.2438348393952777E-5 0.0 0.0 7.853421937883471E-5 1.009725677727875E-4 16-17 2.2438348393952777E-5 0.0 0.0 1.4584926456069305E-4 1.1219174196976388E-4 18-19 3.365752259092917E-5 0.0 0.0 1.7950678715162222E-4 1.1219174196976388E-4 20-21 4.4876696787905554E-5 0.0 0.0 2.916985291213861E-4 1.1219174196976388E-4 22-23 4.4876696787905554E-5 0.0 0.0 4.936436646669611E-4 1.2341091616674025E-4 24-25 4.4876696787905554E-5 0.0 0.0 8.750955873641582E-4 1.3463009036371665E-4 26-27 4.4876696787905554E-5 0.0 0.0 0.0012789858584553082 1.3463009036371665E-4 28-29 4.4876696787905554E-5 0.0 0.0 0.00254675254271364 1.3463009036371665E-4 30-31 4.4876696787905554E-5 0.0 0.0 0.006854915434352573 1.3463009036371665E-4 32-33 4.4876696787905554E-5 0.0 0.0 0.0181975005474957 1.5706843875766942E-4 34-35 4.4876696787905554E-5 0.0 0.0 0.03324241314564104 1.5706843875766942E-4 36-37 4.4876696787905554E-5 0.0 0.0 0.05771143206924654 1.5706843875766942E-4 38-39 4.4876696787905554E-5 0.0 0.0 0.09170552988608499 1.5706843875766942E-4 40-41 4.4876696787905554E-5 0.0 0.0 0.13006388646554726 1.5706843875766942E-4 42-43 4.4876696787905554E-5 0.0 0.0 0.17234895401395128 1.5706843875766942E-4 44-45 4.4876696787905554E-5 0.0 0.0 0.22456299072667937 1.5706843875766942E-4 46-47 4.4876696787905554E-5 0.0 0.0 0.2863694213778223 1.5706843875766942E-4 48-49 4.4876696787905554E-5 2.2438348393952777E-5 0.0 0.34142190916238546 1.7950678715162222E-4 50-51 4.4876696787905554E-5 4.4876696787905554E-5 0.0 0.39458957568185654 1.7950678715162222E-4 52-53 5.609587098488194E-5 4.4876696787905554E-5 0.0 0.44945133750507105 1.907259613485986E-4 54-55 6.731504518185832E-5 4.4876696787905554E-5 0.0 0.5119196994338355 2.01945135545575E-4 56-57 6.731504518185832E-5 4.4876696787905554E-5 0.0 0.5637074075270787 2.01945135545575E-4 58-59 6.731504518185832E-5 4.4876696787905554E-5 0.0 0.6005623947641461 2.01945135545575E-4 60-61 7.853421937883471E-5 6.731504518185832E-5 0.0 0.629530302540739 2.01945135545575E-4 62-63 1.6828761295464582E-4 6.731504518185832E-5 1.1219174196976389E-5 0.6566246082264371 2.01945135545575E-4 64-65 1.7950678715162222E-4 1.1219174196976388E-4 2.2438348393952777E-5 0.6850427764673782 2.01945135545575E-4 66-67 1.7950678715162222E-4 1.1219174196976388E-4 2.2438348393952777E-5 0.7099942198814537 2.1316430974255139E-4 68-69 1.7950678715162222E-4 1.1219174196976388E-4 2.2438348393952777E-5 0.744089290266065 2.2438348393952776E-4 70-71 1.7950678715162222E-4 1.1219174196976388E-4 3.365752259092917E-5 0.7964267378949599 2.2438348393952776E-4 72-73 1.907259613485986E-4 1.1219174196976388E-4 4.4876696787905554E-5 0.8703162191562464 2.3560265813650416E-4 74-75 2.01945135545575E-4 1.1219174196976388E-4 4.4876696787905554E-5 0.9690000753928506 2.4682183233348055E-4 76-77 2.01945135545575E-4 1.1219174196976388E-4 4.4876696787905554E-5 1.1283347873383094 2.4682183233348055E-4 78-79 2.01945135545575E-4 1.1219174196976388E-4 4.4876696787905554E-5 1.367022718378982 2.692601807274333E-4 80-81 2.01945135545575E-4 1.1219174196976388E-4 4.4876696787905554E-5 1.6561071799124725 2.692601807274333E-4 82-83 2.01945135545575E-4 1.1219174196976388E-4 4.4876696787905554E-5 1.9446194635619174 2.692601807274333E-4 84-85 2.01945135545575E-4 1.1219174196976388E-4 4.4876696787905554E-5 2.1733784254382655 2.692601807274333E-4 86-87 2.01945135545575E-4 1.1219174196976388E-4 4.4876696787905554E-5 2.3509667338022053 2.8047935492440967E-4 88-89 2.01945135545575E-4 1.1219174196976388E-4 4.4876696787905554E-5 2.5217225650801858 2.916985291213861E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18170 0.0 27.604374 1 GGTATCA 12320 0.0 25.511816 1 ATCAACG 20720 0.0 23.791683 3 TCAACGC 20740 0.0 23.79142 4 CAACGCA 20790 0.0 23.68895 5 AACGCAG 21040 0.0 23.442415 6 TATCAAC 21525 0.0 23.012987 2 GTACATG 12430 0.0 21.68998 1 TACATGG 11950 0.0 21.65123 2 CGCAGAG 23065 0.0 20.7939 8 ACGCAGA 23270 0.0 20.771975 7 ACATGGG 11635 0.0 20.497232 3 GAGTACT 10540 0.0 20.166788 12-13 AGAGTAC 15855 0.0 20.146585 10-11 AGTACTT 11330 0.0 18.65698 12-13 GCAGAGT 24515 0.0 18.567383 9 GTACTTT 11410 0.0 18.526068 14-15 CATGGGG 7285 0.0 17.627338 4 GAGTACA 9605 0.0 15.283854 1 CAGAGTA 23270 0.0 15.079364 10-11 >>END_MODULE