FastQCFastQC Report
Fri 27 May 2016
SRR939676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4488059
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152150.034.3474581
GTACATG105450.030.3400751
TATCAAC195750.026.0170172
ATCAACG204800.024.7058643
CAACGCA206500.024.5676365
TCAACGC207050.024.5025544
TACATGG118800.024.4567782
AACGCAG212650.024.0346646
ACATGGG118100.023.2816413
GGTATCA110100.023.2656351
ACGCAGA223600.022.4782477
GAGTACT95800.021.87268612-13
CGCAGAG233350.021.3156248
AGAGTAC150900.020.68809310-11
GTACTTT104450.020.0638814-15
GCAGAGT237700.019.7742619
AGTACTT107550.018.78097212-13
GTATAGG11750.018.3858761
CATGGGG75650.018.3578034
CATGGGA58150.016.9777014
Produced by FastQC (version 0.11.5)