FastQCFastQC Report
Fri 27 May 2016
SRR939676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4488059
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112030.24961793060207094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87690.19538513196907617No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63210.14084039447788008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60330.13442336653773937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA177850.026.6388231
GGTATCA125850.025.3096261
GTACATG122350.022.9096091
ATCAACG205350.022.6272323
TCAACGC205500.022.587764
CAACGCA206800.022.4001485
TATCAAC211150.022.0967832
TACATGG122600.021.9719922
AACGCAG211450.021.8943676
ACATGGG121450.020.9742363
AGAGTAC160800.019.86332110-11
ACGCAGA233050.019.5009847
GAGTACT103350.019.36446412-13
CGCAGAG233150.019.2499718
AGTACTT110500.018.53812412-13
GCAGAGT244100.017.6139059
CATGGGG75700.017.2613124
GTACTTT115450.017.11032714-15
GAGTACA101550.015.3344851
CATGGGA55300.015.2692544
Produced by FastQC (version 0.11.5)