##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939676_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4488059 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.498179725355662 31.0 31.0 34.0 30.0 34.0 2 31.70169509803681 31.0 31.0 34.0 30.0 34.0 3 31.760140630949817 31.0 31.0 34.0 30.0 34.0 4 35.554584955322554 37.0 35.0 37.0 33.0 37.0 5 35.3065744010941 37.0 35.0 37.0 33.0 37.0 6 35.30659467711989 37.0 35.0 37.0 32.0 37.0 7 35.214334749164394 37.0 35.0 37.0 32.0 37.0 8 35.17598342624284 37.0 35.0 37.0 32.0 37.0 9 36.788269049047706 39.0 37.0 39.0 32.0 39.0 10-11 36.663018356042116 39.0 35.0 39.0 32.0 39.0 12-13 36.599921703346595 39.0 35.0 39.0 32.0 39.0 14-15 37.69538290383437 39.0 36.5 41.0 32.0 41.0 16-17 37.64226662350028 39.0 36.0 41.0 32.0 41.0 18-19 37.595069160187066 39.0 36.0 41.0 32.0 41.0 20-21 37.52272207205832 39.0 36.0 41.0 32.0 41.0 22-23 37.38722530162816 39.0 36.0 41.0 32.0 41.0 24-25 37.25751221184927 39.0 36.0 41.0 31.0 41.0 26-27 36.976831409747504 39.0 36.0 40.0 31.0 41.0 28-29 36.78962665152129 39.0 36.0 40.0 30.0 41.0 30-31 36.5612567704658 38.0 35.0 40.0 30.0 41.0 32-33 36.417461312340144 38.0 35.0 40.0 30.0 41.0 34-35 36.40021432427693 38.0 35.0 40.0 30.0 41.0 36-37 36.34259320120346 38.0 35.0 40.0 30.0 41.0 38-39 36.41247418984465 38.5 35.0 40.0 30.0 41.0 40-41 36.321139049196994 38.0 35.0 40.0 30.0 41.0 42-43 36.156939558949645 38.0 35.0 40.0 30.0 41.0 44-45 35.97064443671529 38.0 35.0 40.0 29.0 41.0 46-47 35.721329198212416 38.0 34.5 40.0 28.0 41.0 48-49 35.45473911996255 38.0 34.0 40.0 27.0 41.0 50-51 35.229884678432256 38.0 34.0 40.0 27.0 41.0 52-53 35.01118278525304 38.0 34.0 40.0 26.5 41.0 54-55 34.73018692490451 38.0 33.0 40.0 26.0 41.0 56-57 33.98319941872422 37.5 32.5 40.0 24.0 41.0 58-59 30.919068911527233 35.5 28.0 39.0 8.5 40.5 60-61 30.66611724578487 34.5 27.0 39.0 11.0 40.0 62-63 32.38757489596282 35.5 30.5 39.0 20.5 40.0 64-65 32.421359099780105 36.0 31.0 39.0 20.0 40.0 66-67 27.607319778995773 32.0 19.0 37.0 5.0 39.5 68-69 20.009560146156723 20.5 8.0 32.5 2.0 37.0 70-71 18.019949381235854 16.0 7.5 29.0 2.0 35.0 72-73 19.774596880299477 20.0 8.5 31.0 2.0 35.5 74-75 21.267784581263307 24.5 9.5 32.5 2.0 36.0 76-77 23.177969696922432 27.5 12.5 33.0 2.0 35.5 78-79 25.43305936931756 31.0 18.0 34.5 2.0 36.5 80-81 27.029752505481767 32.5 24.0 35.0 2.0 37.0 82-83 28.08315732925971 33.0 27.5 35.0 2.0 36.0 84-85 28.10430099069553 34.0 28.0 35.0 2.0 36.0 86-87 27.823685807160736 33.5 27.0 35.0 2.0 36.0 88-89 27.394639976880875 33.0 26.5 35.0 2.0 35.0 90-91 25.63642679385454 32.5 18.5 34.5 2.0 35.0 92-93 24.07755702409438 31.5 6.0 34.0 2.0 35.0 94-95 20.271940720921897 29.0 2.0 34.0 2.0 35.0 96-97 17.517102493527823 19.5 2.0 33.0 2.0 35.0 98-99 17.802874026388693 23.5 2.0 33.0 2.0 35.0 100-101 17.005321342700707 20.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.19928555587959806 1101 2 0.23404778431073936 1101 3 0.2128531627924808 1101 4 0.22818853066885936 1101 5 0.30912055503213764 1101 6 0.3453562248651565 1101 7 0.31926735531844486 1101 8 0.38497625714492045 1101 9 0.3851874300213254 1101 10-11 0.3287304673657587 1101 12-13 0.26493036908881606 1101 14-15 0.38754897100714203 1101 16-17 0.38021300699731597 1101 18-19 0.3884104007231315 1101 20-21 0.35043656450175575 1101 22-23 0.2850816959942861 1101 24-25 0.27184268190559635 1101 26-27 0.3214618361415802 1101 28-29 0.257285982456267 1101 30-31 0.19162011995994988 1101 32-33 0.1881644586515776 1101 34-35 0.05610449738975376 1101 36-37 0.0467705151721276 1101 38-39 0.10688738933900055 1101 40-41 0.10463423291335516 1101 42-43 0.20158639726665228 1101 44-45 0.13612587208818638 1101 46-47 0.03805900757834024 1101 48-49 0.008583694309130863 1101 50-51 -0.09351403750006426 1101 52-53 -0.14170311394636315 1101 54-55 -0.12147341742488038 1101 56-57 -3.4895842747501504 1101 58-59 -10.365181357925277 1101 60-61 -12.062561815315242 1101 62-63 -4.367958694585994 1101 64-65 -1.5377998878528771 1101 66-67 -2.3658123801466324 1101 68-69 -4.870534922648453 1101 70-71 -4.715890917962973 1101 72-73 -3.6831504934165835 1101 74-75 -6.535528267161162 1101 76-77 -5.125623147698089 1101 78-79 1.0127004527888275 1101 80-81 1.6290921983128364 1101 82-83 0.23403607367827206 1101 84-85 -0.06517939378411697 1101 86-87 -0.11957692569657041 1101 88-89 -0.2978804444178387 1101 90-91 1.2224224403309556 1101 92-93 0.7453692157575134 1101 94-95 -8.831065476335013 1101 96-97 -9.876120343647695 1101 98-99 -9.230279856379394 1101 100-101 -10.069682105203842 1102 1 0.2816582161150514 1102 2 0.3138390296419402 1102 3 0.2487176801260027 1102 4 0.21354148887200353 1102 5 0.3483423467754534 1102 6 0.27350923508711844 1102 7 0.32182472751758695 1102 8 0.2985681394243045 1102 9 0.22815981789197792 1102 10-11 0.3281116852986301 1102 12-13 0.31122193944351295 1102 14-15 0.3964848587579226 1102 16-17 0.38846774750360424 1102 18-19 0.31951414720705884 1102 20-21 0.31441033527493545 1102 22-23 0.29690694288604647 1102 24-25 0.22947383650875963 1102 26-27 0.13707560170535515 1102 28-29 0.26370997212627856 1102 30-31 0.3015321227182284 1102 32-33 0.15925152956693012 1102 34-35 0.21648274983608928 1102 36-37 -0.11986970922394846 1102 38-39 0.03668085544452282 1102 40-41 0.08921860088286593 1102 42-43 0.011905568646817244 1102 44-45 -0.013842571273229964 1102 46-47 -0.06285921203064504 1102 48-49 0.06995865097579923 1102 50-51 0.012207641698914529 1102 52-53 -0.09937364965358597 1102 54-55 -0.30551514953852177 1102 56-57 -3.7865123886615706 1102 58-59 -10.415169364802903 1102 60-61 -8.288569195249455 1102 62-63 -2.5185256178085567 1102 64-65 -0.7511596992150338 1102 66-67 -1.9698511640882472 1102 68-69 -5.108268626211215 1102 70-71 -4.827761295505901 1102 72-73 -3.5413704025831514 1102 74-75 -4.189380548404539 1102 76-77 -4.348026333229484 1102 78-79 1.6974880498394889 1102 80-81 2.052437326216264 1102 82-83 0.6677742607585593 1102 84-85 0.322519177316984 1102 86-87 0.28240280051583255 1102 88-89 0.16306062846458858 1102 90-91 1.4107204605294612 1102 92-93 1.1368564890268047 1102 94-95 -8.789928925438442 1102 96-97 -9.791823678874334 1102 98-99 -8.878672279247947 1102 100-101 -9.680457316356193 1103 1 0.2375714347472737 1103 2 0.21604663223700626 1103 3 0.3091541832006435 1103 4 0.20425557038797137 1103 5 0.22886627590113306 1103 6 0.2500289810644105 1103 7 0.21235765595483258 1103 8 0.3239780675464985 1103 9 0.3563307260494142 1103 10-11 0.3456652654785515 1103 12-13 0.31412666050860594 1103 14-15 0.29206057403545316 1103 16-17 0.3387212086650848 1103 18-19 0.3298535102249929 1103 20-21 0.27179724587107756 1103 22-23 0.3039331882040699 1103 24-25 0.43119916643489375 1103 26-27 0.391923488544478 1103 28-29 0.3477992038738691 1103 30-31 0.31469999707208274 1103 32-33 0.19247787756752643 1103 34-35 0.17467551450627639 1103 36-37 0.24661759109926606 1103 38-39 0.2690417672191856 1103 40-41 0.1827352313772579 1103 42-43 0.20501518813784259 1103 44-45 0.11076653480488119 1103 46-47 0.010896126296792374 1103 48-49 0.07315125520445775 1103 50-51 -2.78356130742452E-4 1103 52-53 0.09891914302093596 1103 54-55 -0.06188103208364737 1103 56-57 0.44848833332533644 1103 58-59 -8.702882970828504 1103 60-61 -7.983661908578352 1103 62-63 -2.380765228489878 1103 64-65 -0.5453192262333886 1103 66-67 -2.0125920597604114 1103 68-69 -3.5972740053783205 1103 70-71 -4.064958972604185 1103 72-73 -3.150031802391787 1103 74-75 -3.858571799090061 1103 76-77 -0.6361598220452507 1103 78-79 3.22626269220072 1103 80-81 2.738494628639806 1103 82-83 1.155575052172896 1103 84-85 0.7946927808922055 1103 86-87 0.6468606977399034 1103 88-89 0.4295306813227775 1103 90-91 1.7905730876866741 1103 92-93 1.3395718161525103 1103 94-95 -7.44236059922288 1103 96-97 -8.875619740180502 1103 98-99 -8.078099831143492 1103 100-101 -9.493134349004588 1104 1 0.187530407698155 1104 2 0.13266460921503054 1104 3 0.18936291562978624 1104 4 0.10153416267595361 1104 5 0.17483375863890416 1104 6 0.22606114925083176 1104 7 0.19113374085687695 1104 8 0.1455574971907012 1104 9 0.22403525466937424 1104 10-11 0.20803641074517287 1104 12-13 0.19616298567697754 1104 14-15 0.32200353526249614 1104 16-17 0.2734489561470568 1104 18-19 0.2999466006526603 1104 20-21 0.16352508062200144 1104 22-23 0.2414558036332224 1104 24-25 0.21930004508422485 1104 26-27 0.2631946033733854 1104 28-29 0.17514743039851766 1104 30-31 0.28736033780411674 1104 32-33 0.3675252901495867 1104 34-35 0.14143602190072357 1104 36-37 0.10217510665868446 1104 38-39 0.1582043471447676 1104 40-41 0.14158765532555861 1104 42-43 0.18616190214259376 1104 44-45 0.29402121965755157 1104 46-47 0.1825927304703825 1104 48-49 0.06718091494068545 1104 50-51 0.19936639538688183 1104 52-53 0.2174025736272256 1104 54-55 0.16036478113525732 1104 56-57 0.6072748532524628 1104 58-59 -8.448062691653003 1104 60-61 -7.727310830913243 1104 62-63 -2.2641282811577526 1104 64-65 -0.390810481246028 1104 66-67 -1.5418260937995605 1104 68-69 -3.35168002310456 1104 70-71 -3.8299313179252934 1104 72-73 -2.8776690008882078 1104 74-75 -3.6412413828810237 1104 76-77 -0.1297294573461798 1104 78-79 3.764218699071556 1104 80-81 3.121287772493094 1104 82-83 1.4723212985437861 1104 84-85 1.1081293257229028 1104 86-87 1.1573678261231706 1104 88-89 0.8134922446715258 1104 90-91 2.268159334951534 1104 92-93 1.9918026552966808 1104 94-95 -6.5988046928177635 1104 96-97 -7.915770108838604 1104 98-99 -5.896188635814296 1104 100-101 -5.402288037075561 1105 1 0.16149372105310178 1105 2 0.21459181117814907 1105 3 0.20356596965435614 1105 4 0.19770795134035524 1105 5 0.2516313601957165 1105 6 0.2638876767165854 1105 7 0.15657164589863726 1105 8 0.14516077976182373 1105 9 0.19945406234045038 1105 10-11 0.2001766896134214 1105 12-13 0.20540512841751024 1105 14-15 0.29147365448502427 1105 16-17 0.4134041796795742 1105 18-19 0.3318776894619049 1105 20-21 0.32171868183677077 1105 22-23 0.3571996034658298 1105 24-25 0.2996293646011168 1105 26-27 0.23254941282723252 1105 28-29 0.37002080990757946 1105 30-31 0.3642045815145565 1105 32-33 0.23178392607388787 1105 34-35 0.3284456638703688 1105 36-37 0.34930078394162223 1105 38-39 0.36071952527893103 1105 40-41 0.3031563346011552 1105 42-43 0.3716744605424509 1105 44-45 0.41826801365729693 1105 46-47 0.3362085338685219 1105 48-49 0.25361284201309076 1105 50-51 0.3284039863714696 1105 52-53 0.3764581477532687 1105 54-55 0.3490144130509307 1105 56-57 0.914246430042283 1105 58-59 -8.619207652875236 1105 60-61 -7.681317003224908 1105 62-63 -2.049580852565594 1105 64-65 -0.16908715731887014 1105 66-67 -1.6407715924430484 1105 68-69 -3.515715294937621 1105 70-71 -3.851995906943138 1105 72-73 -2.866741910348015 1105 74-75 -3.558916556558266 1105 76-77 0.027309750566402613 1105 78-79 4.046396343487324 1105 80-81 3.461011080872872 1105 82-83 1.8776607886413963 1105 84-85 1.4484527631459763 1105 86-87 1.2697219074411734 1105 88-89 1.314560997797198 1105 90-91 2.6624711947555895 1105 92-93 2.3897400356142064 1105 94-95 -6.222855460210752 1105 96-97 -7.730686658252079 1105 98-99 -5.671802692722906 1105 100-101 -5.155572422059896 1106 1 0.10509905772758543 1106 2 0.15398200011109253 1106 3 0.17591514035103373 1106 4 0.07260398601498252 1106 5 0.09403062269765883 1106 6 0.19011164081958043 1106 7 0.19642659103193694 1106 8 0.2072348911861681 1106 9 0.12665312315093757 1106 10-11 0.10821837251472743 1106 12-13 0.14720008052852762 1106 14-15 0.17660820458828397 1106 16-17 0.2293315656615107 1106 18-19 0.19427420162578812 1106 20-21 0.14992836455471092 1106 22-23 0.18062471659894896 1106 24-25 0.18557271782347584 1106 26-27 0.15388656098132714 1106 28-29 0.3242979675859914 1106 30-31 0.286308259073067 1106 32-33 0.24391482688599808 1106 34-35 0.14282292876103497 1106 36-37 0.27409113432959487 1106 38-39 0.3916701236047899 1106 40-41 0.33860399031995314 1106 42-43 0.3324773045957343 1106 44-45 0.33654439207325026 1106 46-47 0.22685611115173998 1106 48-49 0.2658117372939657 1106 50-51 0.27656749906250155 1106 52-53 0.40745101979379683 1106 54-55 0.2943502980867976 1106 56-57 0.9379232755063924 1106 58-59 -8.0724245297645 1106 60-61 -7.59245014250661 1106 62-63 -1.923151886412576 1106 64-65 -0.1519512778855372 1106 66-67 0.7502780944513354 1106 68-69 -2.2336693999445103 1106 70-71 -2.158037355556381 1106 72-73 -0.747052291091574 1106 74-75 -0.6528306696124382 1106 76-77 2.6322970302528645 1106 78-79 4.832205999720529 1106 80-81 3.647223610700035 1106 82-83 2.0572965725768775 1106 84-85 1.5918887503008783 1106 86-87 1.4438712762640549 1106 88-89 1.3226581967816067 1106 90-91 2.732194047263615 1106 92-93 2.196207659296654 1106 94-95 -6.31075333608392 1106 96-97 -7.737748278557724 1106 98-99 -5.584381287452953 1106 100-101 -4.922278815831909 1107 1 0.13128645927319837 1107 2 0.11628098859864622 1107 3 0.10327322702737973 1107 4 0.10122556353422851 1107 5 0.11406729782621738 1107 6 0.10273409868818817 1107 7 0.14683483725914215 1107 8 0.1506516241574687 1107 9 0.09436390810390805 1107 10-11 0.07276949532171528 1107 12-13 0.14665455814647999 1107 14-15 0.16206116270594606 1107 16-17 0.1655629414559101 1107 18-19 0.2576130820245197 1107 20-21 0.25526234917369806 1107 22-23 0.24249706145253924 1107 24-25 0.15649284664770846 1107 26-27 0.19203404310483307 1107 28-29 0.1914922329809059 1107 30-31 0.212292877727279 1107 32-33 0.19080344182931697 1107 34-35 0.18786744366999386 1107 36-37 0.1933004414642312 1107 38-39 0.17567802297832458 1107 40-41 0.12650422742083833 1107 42-43 0.13560705796908934 1107 44-45 0.08310083138587032 1107 46-47 0.04761481488716868 1107 48-49 0.05446779737980734 1107 50-51 0.08695433747375603 1107 52-53 0.18159858237219595 1107 54-55 0.16784672461805883 1107 56-57 0.7228063503659286 1107 58-59 -8.650971067307719 1107 60-61 -7.657374370706929 1107 62-63 -2.1700019486695368 1107 64-65 -0.38439457485043604 1107 66-67 0.5749298839451065 1107 68-69 -2.518585221938732 1107 70-71 -2.5275826408126 1107 72-73 -1.0226471481956274 1107 74-75 -0.9272127399079046 1107 76-77 2.1929466976658176 1107 78-79 4.371371801372465 1107 80-81 3.2192168980361124 1107 82-83 1.5237311543157617 1107 84-85 1.1326633380589222 1107 86-87 1.0617119235535597 1107 88-89 0.9528148835096353 1107 90-91 2.2314375593622273 1107 92-93 1.9062012578136276 1107 94-95 -6.830132055616099 1107 96-97 -8.302344786067497 1107 98-99 -6.108812852465949 1107 100-101 -5.409552252827378 1108 1 -0.046205699702010605 1108 2 -0.07161913631760797 1108 3 -0.05679064978873072 1108 4 -0.048386216663928394 1108 5 -0.03992480794813957 1108 6 -0.07452626238128346 1108 7 -0.06550770367216785 1108 8 -0.01579253170443451 1108 9 -0.060498365137625854 1108 10-11 -0.13736256644073563 1108 12-13 -0.133438882562551 1108 14-15 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2314 70-71 -3.5386864922910863 2314 72-73 -3.0145049018865073 2314 74-75 -4.053329373324853 2314 76-77 0.6696770042843561 2314 78-79 3.4803817427757586 2314 80-81 2.64556577836273 2314 82-83 1.0764652144371105 2314 84-85 0.716014641038349 2314 86-87 0.6777712514167611 2314 88-89 0.8208108028731367 2314 90-91 2.387081766476843 2314 92-93 4.0238421494306635 2314 94-95 6.6783752603005375 2314 96-97 3.8652262940616993 2314 98-99 6.411883423166341 2314 100-101 6.789540561921543 2315 1 -0.43540879621127004 2315 2 -0.3673670883067359 2315 3 -0.4325624552373135 2315 4 -0.27067032925300794 2315 5 -0.4509477376645492 2315 6 -0.47745303307380027 2315 7 -0.4767123174465695 2315 8 -0.6457199300043612 2315 9 -0.625421998418517 2315 10-11 -0.5089704922435843 2315 12-13 -0.5487998514170229 2315 14-15 -0.7309204148241477 2315 16-17 -0.800627908392606 2315 18-19 -0.593631408289454 2315 20-21 -0.6880320723261022 2315 22-23 -0.6414617801280897 2315 24-25 -0.5793972843020327 2315 26-27 -0.5600542853236945 2315 28-29 -0.6031264542327435 2315 30-31 -0.6084637453389661 2315 32-33 -0.4911226593743123 2315 34-35 -0.5780751072210819 2315 36-37 -0.536848151275997 2315 38-39 -0.7860735430779329 2315 40-41 -0.6775959042915076 2315 42-43 -0.7320730603702188 2315 44-45 -0.797784129762519 2315 46-47 -0.6866281712655962 2315 48-49 -0.6039798507446434 2315 50-51 -0.46430613556513833 2315 52-53 -0.4214304382200851 2315 54-55 -0.3534659448330828 2315 56-57 0.2768397096736521 2315 58-59 3.43487275244269 2315 60-61 3.5429335228437786 2315 62-63 0.7654002767244847 2315 64-65 0.09895140209391684 2315 66-67 -1.8176713386958312 2315 68-69 -4.686098021250011 2315 70-71 -3.606177048817722 2315 72-73 -3.197488522514085 2315 74-75 -0.775997422904755 2315 76-77 1.8754726545865488 2315 78-79 3.6167454304450537 2315 80-81 2.553955226233608 2315 82-83 0.8234356614955267 2315 84-85 0.5566832725885931 2315 86-87 0.4244993613292394 2315 88-89 0.5621904518353098 2315 90-91 2.1759125975408793 2315 92-93 3.9213961809071805 2315 94-95 6.521289658513179 2315 96-97 3.9956721357165534 2315 98-99 6.428590633616345 2315 100-101 6.702887875636295 2316 1 -0.49272373877017017 2316 2 -0.40947123264070484 2316 3 -0.6021554804945204 2316 4 -0.3690766800659162 2316 5 -0.5838657285515083 2316 6 -0.6162667993921502 2316 7 -0.46477945106525453 2316 8 -0.510301921302144 2316 9 -0.6435659231463617 2316 10-11 -0.6759441657431324 2316 12-13 -0.6302484719713277 2316 14-15 -0.7017009994443342 2316 16-17 -0.8266686106553252 2316 18-19 -0.7778750974751034 2316 20-21 -0.7607001843473853 2316 22-23 -0.9880945891348532 2316 24-25 -0.8384005401014889 2316 26-27 -0.9068539356243335 2316 28-29 -0.8934748531652446 2316 30-31 -0.8514596252827573 2316 32-33 -0.6202565954552739 2316 34-35 -0.599087703041242 2316 36-37 -0.5652272709504444 2316 38-39 -0.7826875320097031 2316 40-41 -0.732025246551089 2316 42-43 -0.6476817741384551 2316 44-45 -0.6486906397816199 2316 46-47 -0.6426110453932452 2316 48-49 -0.7280452237258075 2316 50-51 -0.6806207369383017 2316 52-53 -0.5455781384250216 2316 54-55 -0.35839588826804203 2316 56-57 0.3737814390038352 2316 58-59 3.517818269315317 2316 60-61 3.6170810364126886 2316 62-63 0.974896630554106 2316 64-65 0.35046171169820184 2316 66-67 -1.6898204215909196 2316 68-69 -2.804792216843282 2316 70-71 -3.001932817445006 2316 72-73 -0.4749926302975567 2316 74-75 5.837109927006608 2316 76-77 6.081613179597831 2316 78-79 5.0291312982748515 2316 80-81 3.314668388495985 2316 82-83 1.6439369575601752 2316 84-85 1.2490197071339644 2316 86-87 1.0532911502189393 2316 88-89 0.9991090594129339 2316 90-91 2.5406622030696155 2316 92-93 4.219298757732318 2316 94-95 6.904928281250928 2316 96-97 3.962587167800418 2316 98-99 6.317759771890678 2316 100-101 6.4889549195942795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 31.0 4 48.0 5 157.0 6 349.0 7 889.0 8 2070.0 9 3575.0 10 5819.0 11 9168.0 12 13049.0 13 17744.0 14 22173.0 15 29318.0 16 39256.0 17 52034.0 18 58142.0 19 60554.0 20 64651.0 21 66699.0 22 66947.0 23 68274.0 24 72680.0 25 78831.0 26 97524.0 27 138263.0 28 194266.0 29 256921.0 30 314056.0 31 354244.0 32 374016.0 33 376473.0 34 384577.0 35 411849.0 36 413952.0 37 311672.0 38 120211.0 39 7573.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.44932555612313 19.813588544215722 14.494993933526427 24.242091966134723 2 15.578951919076491 20.9787104035947 39.51345259952263 23.928885077806182 3 18.990970251249205 24.854959410024925 30.09850148861845 26.05556885010742 4 13.156823473131704 17.48406159544694 37.34659014063764 32.01252479078372 5 13.767236774401246 37.38820564543098 34.69713751162644 14.147420068541342 6 31.65105449816948 37.01435297530625 17.51861996466624 13.815972561858034 7 27.728779857840554 32.4306119861615 21.79844338053488 18.042164775463068 8 25.288415583142367 35.98033000932475 20.151401887900214 18.57985251963267 9 25.800151022970063 16.658693657993357 20.48965933825736 37.05149598077922 10-11 24.67655839640254 26.469861470181204 28.12561064816661 20.727969485249638 12-13 25.53679664193363 23.78908120414638 28.241128291762653 22.43299386215734 14-15 22.509714708425058 25.307528453400597 26.16147583354872 26.021281004625628 16-17 22.312741432320742 28.209288246879105 26.76139061451732 22.71657970628283 18-19 22.283291912152336 27.351381738406193 28.329102089508073 22.036224259933395 20-21 23.03586026832535 26.561694041900964 28.203038329041576 22.199407360732113 22-23 22.652220035431796 26.483631342636095 28.114113473107196 22.750035148824914 24-25 22.684164368715543 26.6191977105455 28.197887905142004 22.49875001559695 26-27 22.662366063858872 26.682060127186556 28.18590823236713 22.469665576587442 28-29 22.49719057092434 27.003507009730253 28.130587455045514 22.36871496429989 30-31 22.76221868971206 26.654415778822678 27.99699989170732 22.58636563975794 32-33 22.610638207082758 26.619928252382092 28.126574750360113 22.642858790175037 34-35 22.88798427533625 26.728508127763007 27.980685749261667 22.40282184763908 36-37 22.899469047072177 26.54065547376566 28.08494577857858 22.47492970058358 38-39 22.65945918224002 26.85399659731219 28.017700220082105 22.46884400036569 40-41 22.80625741103827 26.731862433290203 27.995040632141006 22.466839523530517 42-43 22.65114241987094 26.769034254421598 27.760527458373076 22.81929586733439 44-45 22.762785987572077 26.70813693439587 27.790063571315716 22.739013506716336 46-47 22.918237403277747 26.859044131371224 27.576905605949207 22.64581285940182 48-49 22.91799987424183 26.84777552968941 27.59717383034866 22.637050765720108 50-51 22.640655234101445 26.985285682768335 27.575914088873187 22.798144994257036 52-53 22.86058122914187 27.06457223440404 27.247851220535274 22.82699531591882 54-55 22.85393253668068 26.803449905076448 27.325855440457413 23.01676211778545 56-57 22.709935535482686 26.96414106877813 27.45883427872225 22.867089117016935 58-59 21.89376520122961 26.64840910814815 28.948706635960875 22.509119054661365 60-61 22.161005898698964 26.852589311873597 28.424353536955767 22.562051252471672 62-63 22.751189413020885 26.858764222598936 27.617009452914576 22.773036911465603 64-65 22.654331330796477 26.979389809299253 27.468764302110483 22.897514557793784 66-67 22.41492578606037 26.599324930485686 28.210815711249403 22.774933572204546 68-69 21.535350492193153 26.090687700991232 29.954980246085167 22.41898156073045 70-71 22.41412574858976 26.023501003520167 29.016684870480237 22.545688377409835 72-73 21.832808770403236 26.265324871794206 29.143611256943853 22.758255100858708 74-75 21.928648447087763 26.548787223587446 28.632308185763954 22.89025614356084 76-77 21.001573784285032 26.898668428760303 28.76807183307762 23.33168595387704 78-79 22.290033006169864 26.822123657618747 27.816734524758434 23.071108811452955 80-81 22.511927015923973 26.89666217414898 27.32359066621775 23.2678201437093 82-83 22.707202761466867 26.948365876980635 27.180370893859347 23.164060467693147 84-85 22.662094322043256 26.934980898492235 27.053886962701508 23.349037816763 86-87 22.530188937797252 26.860528722800993 27.277542148648 23.33174019075376 88-89 22.616388047613025 26.845444827745634 27.035816720262606 23.50235040437873 90-91 22.33896784085216 26.694147270730227 27.529017054205333 23.437867834212277 92-93 22.33777105502667 26.848006313540168 27.294858711687798 23.519363919745363 94-95 21.18589564058975 26.824136867544034 28.34024955071019 23.649717941156027 96-97 20.662545605734213 26.819428764990207 28.59962286231529 23.91840276696029 98-99 21.629261737621434 26.665785310643237 27.813084346892758 23.89186860484257 100-101 20.898760712537072 26.705989385218853 28.300940361601697 24.094309540642378 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 567.0 1 438.0 2 345.0 3 409.0 4 447.0 5 497.0 6 647.5 7 981.5 8 1468.5 9 1921.5 10 2146.5 11 2317.0 12 2570.0 13 2801.5 14 3047.5 15 3446.0 16 3810.0 17 4245.0 18 4813.0 19 5381.0 20 6058.0 21 7030.5 22 8606.5 23 10853.0 24 13664.5 25 17375.0 26 22740.5 27 28839.5 28 35260.5 29 42545.5 30 50400.0 31 59093.0 32 68515.5 33 78197.5 34 88054.0 35 97473.0 36 107066.0 37 115752.5 38 122250.0 39 128031.0 40 133575.0 41 139165.0 42 144628.5 43 150376.5 44 156818.0 45 163370.5 46 170272.0 47 177027.5 48 181795.5 49 181924.0 50 178070.0 51 174005.0 52 170971.0 53 166348.5 54 158794.5 55 148160.5 56 134902.5 57 120520.0 58 105882.5 59 90627.5 60 74674.0 61 59699.5 62 46137.0 63 34470.0 64 24886.0 65 17367.5 66 11766.0 67 7957.0 68 5382.5 69 3440.5 70 2181.0 71 1307.5 72 737.5 73 448.5 74 260.5 75 132.0 76 69.5 77 32.0 78 18.0 79 12.5 80 7.0 81 3.5 82 1.0 83 2.0 84 3.0 85 2.5 86 2.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024576325756858367 2 0.012522117022080148 3 1.1140673507188743E-4 4 0.0 5 0.05773097011425206 6 0.0 7 0.0 8 8.912538805750994E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.233455932731722E-4 16-17 0.0 18-19 3.7878289924441724E-4 20-21 0.0 22-23 0.0 24-25 6.684404104313245E-5 26-27 1.336880820862649E-4 28-29 0.0010583639831829306 30-31 0.004924177690177424 32-33 0.005447789345015296 34-35 0.001726804393614255 36-37 0.08175026219575099 38-39 0.07506585809143775 40-41 0.08058049147749617 42-43 0.0838224274680881 44-45 0.08650732978332058 46-47 0.08239642125916793 48-49 0.07265947261388497 50-51 0.06696658845171154 52-53 0.05676173151912664 54-55 0.04635634246341235 56-57 0.06586366177449984 58-59 0.7131145111951515 60-61 0.3369719515719379 62-63 0.0029188564588834504 64-65 0.0018939144962220862 66-67 1.1008099492453196 68-69 2.3762833777363443 70-71 2.3559293672387107 72-73 1.8131334726214605 74-75 1.2871934170205874 76-77 0.7466367977782823 78-79 0.5695112296874886 80-81 0.03811224406809269 82-83 2.116727966365861E-4 84-85 0.0013925841883985928 86-87 0.001648819679063934 88-89 3.3422020521566223E-5 90-91 0.33613640105889875 92-93 0.054845535675890185 94-95 0.9516363309840623 96-97 1.6611078419423628 98-99 0.646626971704249 100-101 0.5662581530233894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4488059.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.13112515358187 #Duplication Level Percentage of deduplicated Percentage of total 1 68.52364781506272 32.29596621151688 2 15.54182063193953 14.65006986636926 3 6.11750875011599 8.649752115895469 4 3.099468313472895 5.843257159674093 5 1.7859856542225785 4.208775669583307 6 1.1574557760048776 3.273131582317326 7 0.7845609617255229 2.5884068614390126 8 0.5413540588812165 2.0411700721224113 9 0.42043435315340094 1.783398970560436 >10 1.8674722169056703 15.156989860192926 >50 0.10605603915840542 3.4239731146972123 >100 0.05139695736719839 4.164134105748342 >500 0.00193962278221198 0.6258023395504464 >1k 7.096177956516125E-4 0.5744081888844155 >5k 1.419235591302371E-4 0.4709736889139484 >10k+ 4.730785304341237E-5 0.24979019253434476 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11203 0.24961793060207094 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8769 0.19538513196907617 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6321 0.14084039447788008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6033 0.13442336653773937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.2281347014377485E-5 7 0.0 0.0 0.0 0.0 2.2281347014377485E-5 8 0.0 0.0 0.0 0.0 2.2281347014377485E-5 9 0.0 0.0 0.0 0.0 2.2281347014377485E-5 10-11 0.0 0.0 0.0 2.2281347014377485E-5 2.2281347014377485E-5 12-13 0.0 0.0 0.0 4.456269402875497E-5 2.2281347014377485E-5 14-15 0.0 0.0 0.0 7.798471455032119E-5 4.456269402875497E-5 16-17 1.1140673507188742E-5 0.0 0.0 1.2254740857907615E-4 4.456269402875497E-5 18-19 2.2281347014377485E-5 0.0 0.0 1.5596942910064239E-4 4.456269402875497E-5 20-21 2.2281347014377485E-5 0.0 0.0 2.116727966365861E-4 4.456269402875497E-5 22-23 2.2281347014377485E-5 0.0 0.0 6.238777164025695E-4 7.798471455032119E-5 24-25 2.2281347014377485E-5 0.0 0.0 0.0010917860037044968 1.1140673507188743E-4 26-27 2.2281347014377485E-5 0.0 0.0 0.001470568902948914 1.1140673507188743E-4 28-29 2.2281347014377485E-5 0.0 0.0 0.0027740277032899966 1.2254740857907618E-4 30-31 2.2281347014377485E-5 0.0 0.0 0.007252578453179871 1.3368808208626492E-4 32-33 2.2281347014377485E-5 0.0 0.0 0.017758233570458856 1.3368808208626492E-4 34-35 2.2281347014377485E-5 0.0 0.0 0.032787002131656466 1.5596942910064239E-4 36-37 2.2281347014377485E-5 0.0 0.0 0.05540256935124962 1.6711010260783113E-4 38-39 2.2281347014377485E-5 0.0 0.0 0.08954873365078311 1.7825077611501988E-4 40-41 2.2281347014377485E-5 0.0 0.0 0.12572250052862496 1.7825077611501988E-4 42-43 2.2281347014377485E-5 0.0 0.0 0.16558383033734628 1.7825077611501988E-4 44-45 2.2281347014377485E-5 0.0 0.0 0.2153492188939584 1.8939144962220862E-4 46-47 2.2281347014377485E-5 0.0 0.0 0.2743613664615372 2.116727966365861E-4 48-49 2.2281347014377485E-5 0.0 0.0 0.33462126946192106 2.2281347014377486E-4 50-51 2.2281347014377485E-5 0.0 0.0 0.3990255030069792 2.2281347014377486E-4 52-53 2.2281347014377485E-5 1.1140673507188742E-5 0.0 0.46194802697558124 2.673761641725298E-4 54-55 2.2281347014377485E-5 2.2281347014377485E-5 0.0 0.535064267203261 2.896575111869073E-4 56-57 2.2281347014377485E-5 3.3422020521566223E-5 0.0 0.6073338162443943 2.896575111869073E-4 58-59 2.2281347014377485E-5 4.456269402875497E-5 0.0 0.6612212539986663 2.896575111869073E-4 60-61 2.2281347014377485E-5 4.456269402875497E-5 1.1140673507188742E-5 0.7070094221132119 3.00798184694096E-4 62-63 5.5703367535943715E-5 4.456269402875497E-5 2.2281347014377485E-5 0.7460463420824013 3.1193885820128477E-4 64-65 1.0026606156469868E-4 4.456269402875497E-5 2.2281347014377485E-5 0.7934721892025038 3.1193885820128477E-4 66-67 1.1140673507188743E-4 4.456269402875497E-5 2.2281347014377485E-5 0.8341022254832211 3.1193885820128477E-4 68-69 1.1140673507188743E-4 4.456269402875497E-5 2.2281347014377485E-5 0.8838676140398332 3.3422020521566226E-4 70-71 1.1140673507188743E-4 4.456269402875497E-5 2.2281347014377485E-5 0.9612061695267375 3.5650155223003975E-4 72-73 1.2254740857907618E-4 4.456269402875497E-5 2.2281347014377485E-5 1.0612494176212923 3.7878289924441724E-4 74-75 1.3368808208626492E-4 4.456269402875497E-5 2.2281347014377485E-5 1.2016887478529137 3.7878289924441724E-4 76-77 1.3368808208626492E-4 4.456269402875497E-5 2.2281347014377485E-5 1.3845517627999095 3.89923572751606E-4 78-79 1.3368808208626492E-4 4.456269402875497E-5 2.2281347014377485E-5 1.6144395606207493 4.0106424625879474E-4 80-81 1.3368808208626492E-4 4.456269402875497E-5 2.2281347014377485E-5 1.884990816742828 4.122049197659835E-4 82-83 1.3368808208626492E-4 4.456269402875497E-5 2.2281347014377485E-5 2.1622598989897415 4.3448626678036097E-4 84-85 1.3368808208626492E-4 4.456269402875497E-5 2.2281347014377485E-5 2.4144401844984658 4.456269402875497E-4 86-87 1.3368808208626492E-4 4.456269402875497E-5 2.2281347014377485E-5 2.647681325044969 4.456269402875497E-4 88-89 1.3368808208626492E-4 4.456269402875497E-5 2.2281347014377485E-5 2.8810338723265447 4.456269402875497E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17785 0.0 26.638823 1 GGTATCA 12585 0.0 25.309626 1 GTACATG 12235 0.0 22.909609 1 ATCAACG 20535 0.0 22.627232 3 TCAACGC 20550 0.0 22.58776 4 CAACGCA 20680 0.0 22.400148 5 TATCAAC 21115 0.0 22.096783 2 TACATGG 12260 0.0 21.971992 2 AACGCAG 21145 0.0 21.894367 6 ACATGGG 12145 0.0 20.974236 3 AGAGTAC 16080 0.0 19.863321 10-11 ACGCAGA 23305 0.0 19.500984 7 GAGTACT 10335 0.0 19.364464 12-13 CGCAGAG 23315 0.0 19.249971 8 AGTACTT 11050 0.0 18.538124 12-13 GCAGAGT 24410 0.0 17.613905 9 CATGGGG 7570 0.0 17.261312 4 GTACTTT 11545 0.0 17.110327 14-15 GAGTACA 10155 0.0 15.334485 1 CATGGGA 5530 0.0 15.269254 4 >>END_MODULE