FastQCFastQC Report
Fri 27 May 2016
SRR939675_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939675_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980729
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82160.2756372686010704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58620.19666329948143557No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49220.16512738997741827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41620.13963027165502132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150100.023.8373621
TACATGG69550.023.8119982
GTACATG72850.023.4498731
ACATGGG71350.022.4128043
TATCAAC170350.020.9201772
GAGTACT76700.020.9092212-13
ATCAACG171150.020.8497663
CAACGCA171800.020.7705355
TCAACGC172150.020.6731764
AACGCAG175000.020.4429026
CATGGGG45600.019.5634754
AGAGTAC111250.019.21403510-11
AGTACTT83450.019.01893812-13
GGTATCA114700.018.8241561
GTACTTT85250.018.81390414-15
ACGCAGA188200.018.6561187
CGCAGAG186350.018.4094168
GCAGAGT191500.016.378119
TATACCG1750.002185589616.2691975
GTATATA17000.015.6317161
Produced by FastQC (version 0.11.5)