FastQCFastQC Report
Fri 27 May 2016
SRR939675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980729
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86210.28922454875971615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71070.23843160515430958No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52100.1747894558680108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47020.15774664519988232No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31000.10400140368346132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143050.024.61531
GGTATCA104450.023.481221
ATCAACG163450.021.2198643
TCAACGC165300.021.0393184
CAACGCA165550.021.0075455
AACGCAG165650.020.9645756
TATCAAC170000.020.5143852
TACATGG72300.020.505082
GAGTACT78850.020.17407812-13
ACATGGG71000.020.1500113
GTACATG76550.019.8631761
AGAGTAC116800.019.6742910-11
AGTACTT83600.019.56183812-13
CGCAGAG175400.018.9954558
ACGCAGA180300.018.8701747
GTACTTT87050.018.21987314-15
GCAGAGT185850.016.9918149
CATGGGG45200.016.6587184
TACTTTT105600.015.17507514-15
GAGTACA68700.014.86943151
Produced by FastQC (version 0.11.5)