##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939675_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2980729 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.566643931736163 31.0 31.0 34.0 30.0 34.0 2 31.781553103284466 31.0 31.0 34.0 30.0 34.0 3 31.82706613046674 31.0 31.0 34.0 30.0 34.0 4 35.61451745529366 37.0 35.0 37.0 33.0 37.0 5 35.34116184329404 37.0 35.0 37.0 33.0 37.0 6 35.35990155428421 37.0 35.0 37.0 33.0 37.0 7 35.28749745448177 37.0 35.0 37.0 32.0 37.0 8 35.251729694313035 37.0 35.0 37.0 32.0 37.0 9 36.87389393668462 39.0 37.0 39.0 33.0 39.0 10-11 36.753055879954196 39.0 36.0 39.0 32.0 39.0 12-13 36.70163305687971 39.0 35.0 39.0 32.0 39.0 14-15 37.78273720958866 40.0 37.0 41.0 32.0 41.0 16-17 37.743401194808385 39.5 37.0 41.0 32.0 41.0 18-19 37.71992707153183 39.5 37.0 41.0 32.0 41.0 20-21 37.6525507686207 39.0 37.0 41.0 32.0 41.0 22-23 37.518707168615464 39.0 36.0 41.0 32.0 41.0 24-25 37.39531503870362 39.0 36.0 41.0 31.5 41.0 26-27 37.14138521147008 39.0 36.0 40.5 31.0 41.0 28-29 36.94469205352114 39.0 36.0 40.0 31.0 41.0 30-31 36.72469000033213 38.5 35.5 40.0 30.0 41.0 32-33 36.554311713678096 38.0 35.0 40.0 30.0 41.0 34-35 36.537754522467495 38.0 35.0 40.0 30.0 41.0 36-37 36.44883281908553 38.5 35.0 40.0 30.0 41.0 38-39 36.483643430851984 39.0 35.0 40.0 30.0 41.0 40-41 36.40333941797459 39.0 35.0 40.0 30.0 41.0 42-43 36.2272616866545 38.5 35.0 40.0 30.0 41.0 44-45 36.029830454227806 38.0 35.0 40.0 29.0 41.0 46-47 35.755213238103835 38.0 35.0 40.0 28.5 41.0 48-49 35.480366044682356 38.0 34.0 40.0 27.5 41.0 50-51 35.24478156182599 38.0 34.0 40.0 27.0 41.0 52-53 35.01436209061609 38.0 34.0 40.0 26.5 41.0 54-55 34.730855941616966 38.0 34.0 40.0 26.0 41.0 56-57 32.715815828946546 37.0 30.5 39.5 16.5 41.0 58-59 27.66006117966444 32.5 18.0 38.5 7.0 40.0 60-61 28.408298104255707 33.0 20.5 38.0 9.5 40.0 62-63 31.518283111279153 35.0 29.5 38.5 15.0 40.0 64-65 31.775166913865704 35.5 31.0 39.0 10.0 40.0 66-67 25.04924785178391 28.0 18.0 35.5 2.0 39.0 68-69 17.48599335934263 13.0 7.5 28.0 2.0 35.0 70-71 16.346848036168332 12.5 7.5 26.5 2.0 33.5 72-73 17.496569295632042 13.5 7.0 28.5 2.0 34.0 74-75 18.50640329932711 17.5 9.0 30.0 2.0 34.5 76-77 19.71026148301305 21.5 8.0 30.0 2.0 34.5 78-79 22.83206021077394 27.5 10.5 33.5 2.0 35.5 80-81 26.527525313438424 31.5 23.5 34.5 2.0 36.0 82-83 27.854298394788657 33.5 27.5 35.0 2.0 36.0 84-85 27.90507657690451 34.0 28.0 35.0 2.0 36.0 86-87 27.674153034375145 34.0 27.5 35.0 2.0 36.0 88-89 27.26108931741195 33.5 26.5 35.0 2.0 35.5 90-91 25.926310644141083 33.0 22.0 35.0 2.0 35.0 92-93 23.486360048162716 31.5 2.0 34.5 2.0 35.0 94-95 16.035542144220422 7.0 2.0 32.0 2.0 35.0 96-97 13.416108777416532 2.0 2.0 30.0 2.0 34.0 98-99 13.092204625110167 2.0 2.0 30.0 2.0 34.0 100-101 12.808153810695302 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38366532803758346 1101 2 0.44055558820727825 1101 3 0.39730263052215165 1101 4 0.337456982531279 1101 5 0.4429056235586515 1101 6 0.49582208958580765 1101 7 0.427832357145455 1101 8 0.5279092181063234 1101 9 0.5099626720272923 1101 10-11 0.5565527899799747 1101 12-13 0.5766505044211527 1101 14-15 0.7836140614068299 1101 16-17 0.6107456769643207 1101 18-19 0.7006959130747603 1101 20-21 0.6143891232521455 1101 22-23 0.6902176875142985 1101 24-25 0.6790097908686974 1101 26-27 0.5847646357979457 1101 28-29 0.7249318600259613 1101 30-31 0.5700892350514977 1101 32-33 0.6819343735286267 1101 34-35 0.7963668454844068 1101 36-37 0.359817076399203 1101 38-39 0.5728825054367519 1101 40-41 0.7421072183590667 1101 42-43 0.796659305448749 1101 44-45 0.5896279421718802 1101 46-47 0.4978023537654934 1101 48-49 0.6985687696385412 1101 50-51 0.6881255662734134 1101 52-53 0.5975723271426361 1101 54-55 0.6399905779449497 1101 56-57 -1.67737501926306 1101 58-59 -9.715666393273008 1101 60-61 -10.063536855487882 1101 62-63 -3.398357983923482 1101 64-65 -4.762953227236121 1101 66-67 -9.558795991557947 1101 68-69 -4.414276723811277 1101 70-71 -4.257019468115006 1101 72-73 -4.416224982355029 1101 74-75 -6.064518685866158 1101 76-77 -7.570939685363832 1101 78-79 -10.835653794841186 1101 80-81 -7.533035464860532 1101 82-83 -3.6479089510715816 1101 84-85 -2.746527176294723 1101 86-87 -2.5785782663411076 1101 88-89 -2.595478858927965 1101 90-91 -9.543878161949888 1101 92-93 -17.37325832804612 1101 94-95 -11.356394314122898 1101 96-97 -9.325034306911334 1101 98-99 -9.410295857308338 1101 100-101 -9.396740498343762 1102 1 0.3231292354112796 1102 2 0.3144104418374347 1102 3 0.30094034862750263 1102 4 0.27095696334781394 1102 5 0.19338201297449586 1102 6 0.27413795175473155 1102 7 0.2607971192491547 1102 8 0.32198962667153097 1102 9 0.454437727126475 1102 10-11 0.47901774818133447 1102 12-13 0.3699872872259462 1102 14-15 0.42004447657967603 1102 16-17 0.47232790795883517 1102 18-19 0.46885945852039157 1102 20-21 0.5582393835078392 1102 22-23 0.5602427752253689 1102 24-25 0.40956415041801364 1102 26-27 0.5130994682836487 1102 28-29 0.5428240127093034 1102 30-31 0.4590153659168408 1102 32-33 0.4073837995166869 1102 34-35 0.31839532653753366 1102 36-37 0.11024124415649084 1102 38-39 0.23467879194395636 1102 40-41 0.27053471764501325 1102 42-43 0.22125096405125078 1102 44-45 0.18896525998223268 1102 46-47 0.2539096234466669 1102 48-49 0.30166498111707796 1102 50-51 0.261060039815149 1102 52-53 0.061931311612546835 1102 54-55 -5.288463805328547E-4 1102 56-57 -2.881381326360515 1102 58-59 -10.498506356270234 1102 60-61 -10.65325979294552 1102 62-63 -4.589092795946829 1102 64-65 -5.617866006408843 1102 66-67 -6.72597590476342 1102 68-69 -4.288974584215325 1102 70-71 -4.200018462048767 1102 72-73 -4.848352013138097 1102 74-75 -5.653863815610336 1102 76-77 -7.7755753167343435 1102 78-79 -10.197640743689538 1102 80-81 -6.070175988014977 1102 82-83 -3.6580676622836776 1102 84-85 -3.2475726752140552 1102 86-87 -3.1660260979699544 1102 88-89 -3.347221357126852 1102 90-91 -9.228876318205614 1102 92-93 -13.666089946235456 1102 94-95 -10.62667869835494 1102 96-97 -9.413138639390649 1102 98-99 -9.443772871252586 1102 100-101 -9.264948219049543 1103 1 0.2420209742613899 1103 2 0.20642961600276877 1103 3 0.2705016615051896 1103 4 0.27039080887323763 1103 5 0.16592827645507668 1103 6 0.25218657596432337 1103 7 0.23658963539782718 1103 8 0.2960676608790749 1103 9 0.4362982579000487 1103 10-11 0.4243382887262044 1103 12-13 0.39873176584323033 1103 14-15 0.6048028072098859 1103 16-17 0.4552301429424759 1103 18-19 0.5277285111452201 1103 20-21 0.3551823996344865 1103 22-23 0.471540735667638 1103 24-25 0.4922827731458881 1103 26-27 0.5436557913931637 1103 28-29 0.529789695038076 1103 30-31 0.48250051474889233 1103 32-33 0.5882308587171181 1103 34-35 0.5850000215649871 1103 36-37 0.4588597471459437 1103 38-39 0.4203792116291112 1103 40-41 0.501978016098029 1103 42-43 0.43498328861844726 1103 44-45 0.37378791497140185 1103 46-47 0.38129187733215986 1103 48-49 0.2949392061211853 1103 50-51 0.3144112137722459 1103 52-53 0.4264058942300508 1103 54-55 0.42714154058679554 1103 56-57 -2.428384411927425 1103 58-59 -10.048176274695933 1103 60-61 -10.528925312285878 1103 62-63 -3.970428620159616 1103 64-65 -4.699053358988426 1103 66-67 -6.149460703190403 1103 68-69 -4.12484134619217 1103 70-71 -4.118668921980449 1103 72-73 -4.424576378504462 1103 74-75 -5.138214975551229 1103 76-77 -6.745941810401021 1103 78-79 -9.208959077683636 1103 80-81 -4.946110818679223 1103 82-83 -2.7376386463412494 1103 84-85 -2.3090192074052673 1103 86-87 -2.123820308076837 1103 88-89 -2.153683627511416 1103 90-91 -5.087313922075687 1103 92-93 -10.070026146482942 1103 94-95 -8.590036840367107 1103 96-97 -8.28903617694406 1103 98-99 -8.589696809324998 1103 100-101 -8.588705732735363 1104 1 0.21784774873236756 1104 2 0.22369255450089653 1104 3 0.17348161496378722 1104 4 0.1871127450570711 1104 5 0.21988857696790376 1104 6 0.23571114821659478 1104 7 0.20785683444092484 1104 8 0.22450162307088561 1104 9 0.21925805875088145 1104 10-11 0.2660094919659599 1104 12-13 0.24456846965868095 1104 14-15 0.3815229032063243 1104 16-17 0.3114276448880915 1104 18-19 0.296573180160685 1104 20-21 0.32512297052706174 1104 22-23 0.3552437677597382 1104 24-25 0.4247194623931918 1104 26-27 0.4097316812323939 1104 28-29 0.4010960619586683 1104 30-31 0.4230776371302909 1104 32-33 0.38478791922222655 1104 34-35 0.35306388884658446 1104 36-37 0.3932105827060255 1104 38-39 0.3117388289723664 1104 40-41 0.2340547186866857 1104 42-43 0.3805950486937988 1104 44-45 0.26004963817910465 1104 46-47 0.3178036551704224 1104 48-49 0.3213002099945186 1104 50-51 0.38398099968432575 1104 52-53 0.38359358312082037 1104 54-55 0.18040611608495993 1104 56-57 -2.960759569908795 1104 58-59 -10.345145300763978 1104 60-61 -10.76856163886291 1104 62-63 -4.388984354196314 1104 64-65 -5.664680830941538 1104 66-67 -6.778306726788248 1104 68-69 -4.25829790179586 1104 70-71 -4.24742069986983 1104 72-73 -4.579529421778258 1104 74-75 -5.291384059380709 1104 76-77 -6.804516476643425 1104 78-79 -9.41154486044696 1104 80-81 -5.1506262423887605 1104 82-83 -2.9297832825144745 1104 84-85 -2.398109675617949 1104 86-87 -2.3042605856895513 1104 88-89 -2.3443982847317884 1104 90-91 -4.759904011388674 1104 92-93 -7.009314658474189 1104 94-95 -8.123361324188153 1104 96-97 -8.117094625896112 1104 98-99 -8.380800557556693 1104 100-101 -8.359600804994667 1105 1 0.29690149858126347 1105 2 0.3186786369111765 1105 3 0.2936481754995093 1105 4 0.22078845776045597 1105 5 0.1673279022733567 1105 6 0.2162430212896993 1105 7 0.1858847153090224 1105 8 0.22817384884138647 1105 9 0.27805682147667454 1105 10-11 0.35467975217237324 1105 12-13 0.36362184511440887 1105 14-15 0.5291312130845611 1105 16-17 0.4116120811106967 1105 18-19 0.4701515252604338 1105 20-21 0.3994928398733748 1105 22-23 0.5246725358127051 1105 24-25 0.5250666644547621 1105 26-27 0.5378866178124113 1105 28-29 0.5412931323639327 1105 30-31 0.5167779328691608 1105 32-33 0.43801060351329824 1105 34-35 0.4825406236432883 1105 36-37 0.5281507607077671 1105 38-39 0.459088077822301 1105 40-41 0.4654904498178283 1105 42-43 0.3226946709057188 1105 44-45 0.33015126688452057 1105 46-47 0.3705902348890362 1105 48-49 0.33793357787990175 1105 50-51 0.30266518541252196 1105 52-53 0.49737451933849997 1105 54-55 0.333464067386565 1105 56-57 1.5506205543199343 1105 58-59 -8.820479295948267 1105 60-61 -10.045123960887349 1105 62-63 -3.808671310751155 1105 64-65 -4.954537758817235 1105 66-67 -6.508328965107264 1105 68-69 -3.8665294112951347 1105 70-71 -4.0524895707603505 1105 72-73 -2.2977473293837374 1105 74-75 -4.378418887780143 1105 76-77 -6.3528698289058205 1105 78-79 -9.033670004850082 1105 80-81 -4.68238414200507 1105 82-83 -2.146925761638091 1105 84-85 -1.5825828616117086 1105 86-87 -1.4264856740288892 1105 88-89 -1.5875311051464962 1105 90-91 -4.276280539222107 1105 92-93 -6.58586616117093 1105 94-95 -8.213265812686192 1105 96-97 -8.333684240547196 1105 98-99 -8.673313138594567 1105 100-101 -8.530713707693376 1106 1 0.27746894981610026 1106 2 0.2160358168139318 1106 3 0.24753181370112642 1106 4 0.18096450082794746 1106 5 0.16249527602028735 1106 6 0.28236787584279455 1106 7 0.2853454542892706 1106 8 0.1651335557596596 1106 9 0.2599848979732968 1106 10-11 0.3178710499012354 1106 12-13 0.21051185439008435 1106 14-15 0.26239663454096984 1106 16-17 0.43006503498201454 1106 18-19 0.22487397065100367 1106 20-21 0.31684041610175484 1106 22-23 0.3605414427670226 1106 24-25 0.42917035649073654 1106 26-27 0.3464940083689143 1106 28-29 0.2918246835508711 1106 30-31 0.3709741240483808 1106 32-33 0.2351620119370068 1106 34-35 0.32839241313507017 1106 36-37 0.36124896613264923 1106 38-39 0.38857176030989393 1106 40-41 0.291696806163408 1106 42-43 0.16746796349192294 1106 44-45 0.13757698575575006 1106 46-47 0.20802222090552647 1106 48-49 0.32973727787076257 1106 50-51 0.40604530932530736 1106 52-53 0.35836497711657955 1106 54-55 0.2373713377651825 1106 56-57 1.6308188384014457 1106 58-59 -8.851922218491403 1106 60-61 -9.945718328047057 1106 62-63 -3.900730233226934 1106 64-65 -5.436465395470098 1106 66-67 -7.124981625891834 1106 68-69 -4.079318786077332 1106 70-71 -4.361069628168757 1106 72-73 -4.669032456092044 1106 74-75 -5.091348635557393 1106 76-77 -6.712645312542314 1106 78-79 -9.192363120955891 1106 80-81 -4.804296265996776 1106 82-83 -2.345086498393986 1106 84-85 -1.7722390935506276 1106 86-87 -1.6959734231862384 1106 88-89 -1.7934095290631369 1106 90-91 -4.477120175830731 1106 92-93 -6.611331481312469 1106 94-95 -7.702080648641434 1106 96-97 -7.389857692809256 1106 98-99 -6.970487356059104 1106 100-101 -7.495652693194994 1107 1 0.027759421410788576 1107 2 0.04903338997061013 1107 3 0.038942756047195104 1107 4 0.07816098143662487 1107 5 -0.0461523444551446 1107 6 -0.018052370964362296 1107 7 -0.002795340103688204 1107 8 0.07719562298041183 1107 9 0.11130092169826611 1107 10-11 -0.03186612015582568 1107 12-13 0.00916818901336569 1107 14-15 0.12238112710583238 1107 16-17 0.08567465052880863 1107 18-19 0.115019487549894 1107 20-21 0.04225925740119152 1107 22-23 -0.05214021910529709 1107 24-25 0.023399107556578258 1107 26-27 0.08352819968662573 1107 28-29 0.028548491914584417 1107 30-31 0.039799025897096385 1107 32-33 0.07739025702007751 1107 34-35 0.1164968985786956 1107 36-37 -0.007461386837185557 1107 38-39 0.0034930987280432646 1107 40-41 -0.012316855371594215 1107 42-43 -0.23393449895759488 1107 44-45 -0.11256942129803349 1107 46-47 -0.13165502767256498 1107 48-49 -0.2261164275160752 1107 50-51 -0.1194342030572102 1107 52-53 -0.1258652263072335 1107 54-55 -0.26183337911871263 1107 56-57 0.8631363800747494 1107 58-59 -9.341140803051431 1107 60-61 -10.475385082167538 1107 62-63 -4.295014970350142 1107 64-65 -5.607800299848723 1107 66-67 -7.027178200201028 1107 68-69 -4.158710060685431 1107 70-71 -3.9360733761893343 1107 72-73 -2.412460536262204 1107 74-75 -4.8096766904284145 1107 76-77 -6.945836611512544 1107 78-79 -7.708214905482187 1107 80-81 -2.681654005190765 1107 82-83 -1.514248722094596 1107 84-85 -1.2906535871461955 1107 86-87 -1.2909353807773876 1107 88-89 -1.1314212509974908 1107 90-91 -0.5048543568413812 1107 92-93 -3.2165255350778246 1107 94-95 -5.939620822736991 1107 96-97 -6.68476834936876 1107 98-99 -6.4459717877186335 1107 100-101 -7.126475008909507 1108 1 -0.1326833767394504 1108 2 -0.1331974589707272 1108 3 -0.14059310240586953 1108 4 -0.10110808455777942 1108 5 -0.06623524344519893 1108 6 -0.16866638027503456 1108 7 -0.13495040452053786 1108 8 -0.051398342096845795 1108 9 -0.1422988432150447 1108 10-11 -0.15133500983521486 1108 12-13 -0.13601717374704947 1108 14-15 -0.2010931293551792 1108 16-17 -0.15935102341214247 1108 18-19 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84-85 1.5202870126904209 2316 86-87 1.3949682641442287 2316 88-89 1.6045543587586053 2316 90-91 2.588391646192811 2316 92-93 4.8304315194209515 2316 94-95 11.727508752864985 2316 96-97 13.663862373780574 2316 98-99 13.460824999148757 2316 100-101 12.211353209085589 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 35.0 5 106.0 6 235.0 7 620.0 8 1450.0 9 2487.0 10 4035.0 11 6485.0 12 9806.0 13 13346.0 14 16652.0 15 22572.0 16 31037.0 17 41561.0 18 46783.0 19 47679.0 20 50105.0 21 50443.0 22 50244.0 23 42735.0 24 43639.0 25 51962.0 26 73636.0 27 109227.0 28 154719.0 29 210111.0 30 261222.0 31 289359.0 32 291596.0 33 268488.0 34 237684.0 35 214999.0 36 174854.0 37 113928.0 38 43663.0 39 3204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.517892348255565 20.020798929918882 14.459987899170493 24.00132082265506 2 15.467633743965411 21.53424545390798 39.31750995713174 23.680610844994863 3 18.520894280576506 25.2685928232105 30.98697630102794 25.223536595185053 4 12.898891512780933 17.804771919889394 37.921126006423265 31.375210560906407 5 13.240404421856358 37.707704986935546 35.27718244575572 13.774708145452372 6 30.998020953934425 37.52877232381743 17.742169784639934 13.731036937608215 7 26.70202490732972 33.19939518151432 22.347284842063804 17.75129506909216 8 24.998616091782804 35.69000321737692 20.675991356360896 18.635389334479378 9 25.498057689914113 16.540450339497486 20.924478541994258 37.03701342859414 10-11 24.221591429479165 26.57537803671518 28.829205875475427 20.37382465833023 12-13 25.106827893444862 24.170865583553553 28.72119538542417 22.001111137577418 14-15 22.23308092715447 25.74470893945959 26.669890729109476 25.352319404276464 16-17 21.673791881113647 28.549777587965895 27.497014991970087 22.27941553895037 18-19 21.59788078261549 27.72275384193701 29.189881112736742 21.48948426271076 20-21 22.60423540684175 26.915311656980556 28.818285728088668 21.662167208089027 22-23 22.069995356814076 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22.245665758649462 48-49 22.2556314460863 27.228496924149624 28.224756061353105 22.29111556841097 50-51 22.066128998130264 27.414344552319236 28.296248131353856 22.223278318196645 52-53 22.419677165728455 27.382368876174557 27.824816665303935 22.373137292793054 54-55 22.362700830980753 27.22835578083166 27.84046566213475 22.568477726052834 56-57 21.2736518733682 27.375813696635642 28.82894310056601 22.521591329430144 58-59 20.81070224857448 26.99301669384584 29.891130500757434 22.30515055682225 60-61 21.948721857278404 27.227705325792805 28.542346176279302 22.28122664064949 62-63 22.14357404707517 27.22147264611995 28.29836271442392 22.33659059238096 64-65 21.678567493779187 27.488424992878986 28.28041949491855 22.552588018423272 66-67 21.358886155301324 27.270530959061784 28.69041587937578 22.680167006261108 68-69 21.46196080923141 26.877510416344723 29.287993564493796 22.37253520993007 70-71 21.398334468389884 26.77289622961124 29.13070883418997 22.6980604678089 72-73 20.625754833518087 26.960879213547013 29.66298513447318 22.75038081846172 74-75 21.16779913001893 27.321800909416737 28.793501192826405 22.716898767737923 76-77 19.59738883413373 27.618256247659577 29.51136681286378 23.272988105342908 78-79 20.534377772810707 27.632976381579393 28.807039467891276 23.025606377718624 80-81 21.809571106261505 27.336238062718593 27.7686076079384 23.085583223081503 82-83 22.122086172060634 27.268137171440817 27.654704522141866 22.955072134356687 84-85 22.01331321070203 27.45766254400987 27.37607161482774 23.152952630460355 86-87 21.815899267155945 27.36387834332422 27.585033260464936 23.2351891290549 88-89 21.95486734095475 27.220500791082973 27.375832180369603 23.44879968759268 90-91 21.186903410796422 27.339292239604468 27.969364766555504 23.504439583043602 92-93 20.281130686563333 27.503541247640783 28.68982342011348 23.525504645682407 94-95 19.305319511264234 27.702448984481837 29.003770433163385 23.98846107109055 96-97 19.214219465358884 27.776146791513888 28.681372815270983 24.328260927856242 98-99 19.55076398298827 27.744669541882438 28.377317964064115 24.32724851106518 100-101 20.048616520472788 27.476428353710165 28.04075930504284 24.434195820774207 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 492.0 1 384.5 2 304.5 3 364.0 4 415.0 5 468.0 6 576.5 7 799.5 8 1076.5 9 1350.5 10 1556.5 11 1741.5 12 1973.5 13 2208.5 14 2489.0 15 2800.5 16 3126.5 17 3492.0 18 3871.0 19 4355.5 20 5088.5 21 5974.0 22 7122.0 23 8888.0 24 11144.0 25 14001.0 26 18124.5 27 23317.0 28 28793.0 29 34146.0 30 39887.5 31 46265.5 32 53242.0 33 60077.5 34 66448.0 35 73102.0 36 79271.5 37 84429.0 38 88409.5 39 92030.0 40 95867.5 41 99610.0 42 102681.0 43 105530.5 44 108656.5 45 111332.0 46 114108.5 47 116777.5 48 118029.5 49 116874.0 50 113304.0 51 109145.5 52 104843.5 53 99943.0 54 93681.5 55 85762.5 56 77450.5 57 68378.5 58 59227.0 59 50098.5 60 41101.5 61 32523.5 62 24436.0 63 17855.5 64 12931.0 65 9112.5 66 6224.5 67 4391.0 68 2953.0 69 1855.0 70 1141.0 71 655.5 72 395.5 73 235.5 74 114.0 75 64.5 76 39.0 77 16.0 78 8.0 79 4.0 80 1.5 81 3.0 82 2.5 83 1.0 84 1.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025765509041580096 2 0.006776865659373932 3 3.0193955908101676E-4 4 0.0 5 0.14533357443766273 6 0.0 7 0.0 8 0.00134195359591563 9 0.0 10-11 0.0 12-13 0.0 14-15 5.871046982130881E-4 16-17 0.0 18-19 2.012930393873445E-4 20-21 0.0 22-23 5.703302782641428E-4 24-25 1.6774419948945375E-5 26-27 4.1936049872363435E-4 28-29 0.004009086367797944 30-31 0.013755024358135207 32-33 0.0032877863099932936 34-35 0.005183295764224121 36-37 0.1095705111065112 38-39 0.10926857154743017 40-41 0.12183261208919026 42-43 0.12411393320224684 44-45 0.11966871191577631 46-47 0.11280797415665765 48-49 0.11208667409885299 50-51 0.11091246470242683 52-53 0.08848506523068685 54-55 0.07583715258918204 56-57 0.5502848464251531 58-59 1.5702031281609297 60-61 0.5202921835564387 62-63 0.005216844604122012 64-65 0.12161454462985397 66-67 1.8924061865402724 68-69 2.8489507097089337 70-71 2.7575468954071303 72-73 2.6091268276988617 74-75 1.996692755362866 76-77 1.4053105800627967 78-79 0.9376565263061486 80-81 0.05248716002025008 82-83 2.1806745933628988E-4 84-85 1.8451861943839912E-4 86-87 4.3613491867257976E-4 88-89 1.6774419948945376E-4 90-91 0.24894916646229834 92-93 0.39552404797618296 94-95 1.9899158897034919 96-97 2.4083705697498834 98-99 1.410762266546204 100-101 0.9403404334979798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2980729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.015698966052724 #Duplication Level Percentage of deduplicated Percentage of total 1 65.92978989851666 28.36015995169696 2 15.456915700193635 13.297800654063668 3 6.639244760096561 8.567752618867699 4 3.607127603116146 6.206524604311338 5 2.1676299767120812 4.662105927401938 6 1.3482568502703014 3.4797726480087423 7 0.9796525853209859 2.949830849103797 8 0.705019784992012 2.4261535069062083 9 0.505020435461919 1.9551426321181287 >10 2.4950466721803535 18.252817610583346 >50 0.10724903776471455 3.183547884000898 >100 0.05459479217602248 4.207908126419144 >500 0.003046039030828986 0.8867537902105525 >1k 0.0011715534732421003 0.8602795161707517 >5k 2.3431069464842008E-4 0.7034496801368116 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8621 0.28922454875971615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7107 0.23843160515430958 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5210 0.1747894558680108 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4702 0.15774664519988232 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3100 0.10400140368346132 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.354883989789075E-5 2 0.0 0.0 0.0 0.0 3.354883989789075E-5 3 0.0 0.0 0.0 0.0 3.354883989789075E-5 4 0.0 0.0 0.0 0.0 6.70976797957815E-5 5 0.0 0.0 0.0 0.0 6.70976797957815E-5 6 0.0 0.0 0.0 0.0 1.0064651969367225E-4 7 0.0 0.0 0.0 0.0 1.0064651969367225E-4 8 0.0 6.70976797957815E-5 0.0 0.0 1.34195359591563E-4 9 0.0 6.70976797957815E-5 0.0 3.354883989789075E-5 2.3484187928523526E-4 10-11 8.387209974472688E-5 6.70976797957815E-5 0.0 3.354883989789075E-5 2.3484187928523526E-4 12-13 1.5096977954050838E-4 6.70976797957815E-5 0.0 5.0323259846836125E-5 2.3484187928523526E-4 14-15 2.012930393873445E-4 6.70976797957815E-5 0.0 1.0064651969367225E-4 3.354883989789075E-4 16-17 2.012930393873445E-4 6.70976797957815E-5 0.0 1.8451861943839912E-4 4.3613491867257976E-4 18-19 2.012930393873445E-4 6.70976797957815E-5 0.0 2.3484187928523526E-4 5.200070184173066E-4 20-21 2.3484187928523526E-4 6.70976797957815E-5 0.0 3.0193955908101676E-4 5.36781438366252E-4 22-23 2.3484187928523526E-4 6.70976797957815E-5 0.0 5.871046982130881E-4 6.542023780088696E-4 24-25 4.02586078774689E-4 6.70976797957815E-5 0.0 0.0013251791759666847 8.387209974472688E-4 26-27 4.3613491867257976E-4 6.70976797957815E-5 0.0 0.002046479233771336 9.058186772430502E-4 28-29 4.696837585704705E-4 6.70976797957815E-5 0.0 0.004814258525347323 9.058186772430502E-4 30-31 4.864581785194159E-4 6.70976797957815E-5 0.0 0.012983401040483719 9.393675171409409E-4 32-33 5.032325984683613E-4 6.70976797957815E-5 0.0 0.03281076542013715 0.0010567884567835586 34-35 5.032325984683613E-4 1.1742093964261762E-4 0.0 0.061209858393701674 0.001073562876732504 36-37 5.200070184173066E-4 1.6774419948945376E-4 0.0 0.10326332920570773 0.0010903372966814494 38-39 5.535558583151974E-4 2.012930393873445E-4 0.0 0.15964215465411313 0.0011071117166303948 40-41 6.374279580599242E-4 2.012930393873445E-4 0.0 0.22375398769898236 0.0011406605565282856 42-43 6.374279580599242E-4 3.0193955908101676E-4 0.0 0.2948943027024597 0.0011574349764772308 44-45 6.542023780088696E-4 3.0193955908101676E-4 0.0 0.3789844699065229 0.0011742093964261762 46-47 6.70976797957815E-4 3.0193955908101676E-4 0.0 0.47604126373112077 0.0013251791759666847 48-49 6.70976797957815E-4 3.354883989789075E-4 0.0 0.5586385075597278 0.0014593745355582478 50-51 6.70976797957815E-4 3.354883989789075E-4 0.0 0.639658955913134 0.001476148955507193 52-53 6.877512179067605E-4 3.354883989789075E-4 0.0 0.7228265300200052 0.0016271187350477014 54-55 7.213000578046511E-4 3.6903723887679824E-4 0.0 0.8181723330098107 0.0018284117744350459 56-57 7.380744777535965E-4 3.6903723887679824E-4 0.0 0.8979011510271481 0.001878735034281882 58-59 7.380744777535965E-4 3.6903723887679824E-4 0.0 0.9524347902811695 0.001878735034281882 60-61 7.380744777535965E-4 3.6903723887679824E-4 0.0 0.9936830889356262 0.001878735034281882 62-63 8.387209974472687E-4 3.6903723887679824E-4 0.0 1.0301674523245823 0.001878735034281882 64-65 8.722698373451595E-4 3.6903723887679824E-4 0.0 1.0708286462808259 0.001878735034281882 66-67 8.722698373451595E-4 3.6903723887679824E-4 0.0 1.1056020188349898 0.0018955094542308275 68-69 8.722698373451595E-4 3.6903723887679824E-4 0.0 1.1533420180096883 0.0019122838741797727 70-71 9.058186772430502E-4 4.02586078774689E-4 0.0 1.2270152704254564 0.0019458327140776635 72-73 9.39367517140941E-4 4.02586078774689E-4 0.0 1.3300269833319298 0.002113576913567117 74-75 9.729163570388318E-4 4.02586078774689E-4 0.0 1.4689527293490956 0.002113576913567117 76-77 0.0010064651969367226 4.02586078774689E-4 0.0 1.693243498486444 0.002130351333516063 78-79 0.0010064651969367226 4.02586078774689E-4 0.0 2.0373539493191095 0.002163900173413953 80-81 0.001023239616885668 4.02586078774689E-4 0.0 2.4529737523941293 0.0023316443729034073 82-83 0.0010400140368346132 4.02586078774689E-4 0.0 2.8593340756573307 0.0026000350920865334 84-85 0.0010400140368346132 4.3613491867257976E-4 0.0 3.1732337961619455 0.0026168095120354786 86-87 0.0010400140368346132 4.864581785194159E-4 0.0 3.4156073900042574 0.0026335839319844238 88-89 0.0010400140368346132 5.032325984683613E-4 0.0 3.6539215742189244 0.0026503583519333694 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14305 0.0 24.6153 1 GGTATCA 10445 0.0 23.48122 1 ATCAACG 16345 0.0 21.219864 3 TCAACGC 16530 0.0 21.039318 4 CAACGCA 16555 0.0 21.007545 5 AACGCAG 16565 0.0 20.964575 6 TATCAAC 17000 0.0 20.514385 2 TACATGG 7230 0.0 20.50508 2 GAGTACT 7885 0.0 20.174078 12-13 ACATGGG 7100 0.0 20.150011 3 GTACATG 7655 0.0 19.863176 1 AGAGTAC 11680 0.0 19.67429 10-11 AGTACTT 8360 0.0 19.561838 12-13 CGCAGAG 17540 0.0 18.995455 8 ACGCAGA 18030 0.0 18.870174 7 GTACTTT 8705 0.0 18.219873 14-15 GCAGAGT 18585 0.0 16.991814 9 CATGGGG 4520 0.0 16.658718 4 TACTTTT 10560 0.0 15.175075 14-15 GAGTACA 6870 0.0 14.8694315 1 >>END_MODULE