FastQCFastQC Report
Fri 27 May 2016
SRR939674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963619
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35160.11863873190177279No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG66350.027.5964281
GTATCAA142400.027.4122161
TACATGG71600.022.6314432
GAGTACT73150.022.00244112-13
TATACCG2154.737194E-821.9523685
TATCAAC175050.021.8871822
ACATGGG71150.021.7789753
ATCAACG181600.020.9420173
CAACGCA182350.020.7840235
TCAACGC184450.020.64234
AACGCAG187050.020.4380666
GTGTAGC14950.020.1884271
GGTATCA110200.020.1759091
AGAGTAC111700.019.56272310-11
ACGCAGA191200.019.3279767
AGTACTT81900.019.2195312-13
GTACTTT83700.019.0354714-15
CGCAGAG195250.018.3952678
CATGGGG45150.018.100484
ACCGTGC6250.017.368428
Produced by FastQC (version 0.11.5)