##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939674_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2963619 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.240870030864292 2.0 2.0 31.0 2.0 33.0 2 22.547413145886836 16.0 16.0 31.0 16.0 34.0 3 27.020278922493073 28.0 25.0 31.0 16.0 34.0 4 32.24954692219209 35.0 32.0 35.0 25.0 37.0 5 32.852841407751804 35.0 33.0 35.0 26.0 37.0 6 33.22980821758802 35.0 33.0 37.0 28.0 37.0 7 33.39654017604827 35.0 33.0 37.0 28.0 37.0 8 33.32542307226401 35.0 33.0 37.0 26.0 37.0 9 34.687681513716846 37.0 34.0 39.0 27.0 39.0 10-11 34.53784899475944 37.0 33.5 39.0 27.0 39.0 12-13 34.557747638950886 37.0 33.5 39.0 27.0 39.0 14-15 35.253121943137764 38.0 33.5 40.0 26.5 41.0 16-17 34.84929523666841 38.0 33.0 40.0 25.0 41.0 18-19 35.08724687620102 38.0 33.5 40.0 26.0 41.0 20-21 35.078567116758265 38.0 34.0 40.0 25.5 41.0 22-23 34.877696829450755 38.0 33.5 40.0 25.0 41.0 24-25 34.68264577869152 38.0 33.0 40.0 24.5 41.0 26-27 34.77852247539242 38.0 33.0 40.0 24.5 41.0 28-29 34.5215295893298 38.0 33.0 40.0 24.0 41.0 30-31 34.40565099629878 38.0 33.0 40.0 24.0 41.0 32-33 34.1046163491326 38.0 33.0 40.0 23.0 41.0 34-35 33.830961402258524 38.0 32.0 40.0 21.0 41.0 36-37 33.56508073406197 38.0 32.0 40.0 19.0 41.0 38-39 33.35971661674459 37.0 31.0 40.0 18.0 41.0 40-41 32.98452719462252 37.0 31.0 40.0 16.0 41.0 42-43 32.74227928758724 37.0 31.0 40.0 15.0 41.0 44-45 32.40713448658549 37.0 30.0 40.0 14.5 41.0 46-47 32.09485547906124 37.0 30.0 39.0 11.5 40.5 48-49 31.734279271390825 36.0 30.0 39.0 9.0 40.0 50-51 31.080696607762334 35.5 29.0 38.5 9.0 40.0 52-53 31.127279012585625 35.5 29.0 39.0 8.0 40.0 54-55 31.2538005053956 36.0 29.0 39.0 7.5 40.0 56-57 30.952918711885708 36.0 28.5 39.0 6.5 40.0 58-59 30.532269667592224 35.0 28.0 39.0 2.0 40.0 60-61 29.950850463571733 34.5 28.0 38.0 2.0 40.0 62-63 29.553983659842913 34.0 27.0 38.0 2.0 40.0 64-65 28.982505004860613 34.0 26.0 38.0 2.0 40.0 66-67 28.382128741919928 33.5 26.0 37.0 2.0 39.0 68-69 28.025735764280093 33.0 25.5 36.5 2.0 39.0 70-71 27.496757849102735 33.0 24.5 36.0 2.0 39.0 72-73 26.902083061284195 32.0 23.0 36.0 2.0 38.5 74-75 26.343019969840928 32.0 22.0 35.0 2.0 38.0 76-77 25.702113699500508 31.0 20.5 35.0 2.0 37.0 78-79 25.026611045481893 31.0 18.5 34.5 2.0 36.5 80-81 24.289304056965488 30.5 15.0 34.0 2.0 36.0 82-83 23.590314409510803 30.0 7.5 34.0 2.0 35.5 84-85 22.884133216854124 29.0 4.5 34.0 2.0 35.0 86-87 22.1961294619855 29.0 2.0 34.0 2.0 35.0 88-89 21.3671963231441 29.0 2.0 33.0 2.0 35.0 90-91 20.386159455719508 26.5 2.0 33.0 2.0 34.0 92-93 19.301571996940226 25.5 2.0 32.0 2.0 34.0 94-95 18.28795857362232 24.5 2.0 32.0 2.0 34.0 96-97 17.359697720928366 21.5 2.0 31.5 2.0 34.0 98-99 16.27472239177843 12.5 2.0 31.0 2.0 34.0 100-101 14.692991069364854 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.9911232609855 1101 2 8.068255336660094 1101 3 3.9402233110847753 1101 4 2.512216467387894 1101 5 1.7845035843266501 1101 6 1.6193559670769488 1101 7 1.2507866484624799 1101 8 1.285652709720182 1101 9 1.237257688631665 1101 10-11 1.261063579445299 1101 12-13 0.985492296346294 1101 14-15 1.0876710590457108 1101 16-17 1.2434115450971959 1101 18-19 1.2518351331998048 1101 20-21 1.140986897604428 1101 22-23 1.102608482177473 1101 24-25 1.3625406637405248 1101 26-27 1.3260417118257521 1101 28-29 1.4784851619504025 1101 30-31 1.351197906734157 1101 32-33 1.1610972121481282 1101 34-35 1.3273663842811487 1101 36-37 1.3650551633921353 1101 38-39 1.1215762481410465 1101 40-41 1.3312317507055624 1101 42-43 1.117641155333068 1101 44-45 1.0132715973630155 1101 46-47 0.8612329535509033 1101 48-49 0.8888744200768137 1101 50-51 0.9481010747618548 1101 52-53 0.8594250043839224 1101 54-55 0.9282361657030442 1101 56-57 0.8630495814747832 1101 58-59 0.9847836054740604 1101 60-61 0.980088460512043 1101 62-63 0.7028678116797238 1101 64-65 0.7223359047672986 1101 66-67 0.5379276600480232 1101 68-69 0.42546863168894333 1101 70-71 0.17997516028095717 1101 72-73 0.3143706705754923 1101 74-75 0.3032896673970278 1101 76-77 0.1754225388834847 1101 78-79 0.1648888786649998 1101 80-81 -0.04872895502367669 1101 82-83 0.008252953795651052 1101 84-85 -0.1851368883202653 1101 86-87 -0.22896757414080682 1101 88-89 -0.10314409951548953 1101 90-91 -0.07253927406239669 1101 92-93 -0.11698942105883603 1101 94-95 -0.1830361708471031 1101 96-97 -0.8302716532726642 1101 98-99 -1.0897663572394443 1101 100-101 -1.1887853543898146 1102 1 14.825635935408712 1102 2 7.930485072347736 1102 3 3.729331191059117 1102 4 2.2026704665858503 1102 5 1.5731651627220202 1102 6 1.282495984664699 1102 7 1.049278669088551 1102 8 0.9970211375232125 1102 9 1.1329884240565065 1102 10-11 1.0469421641839958 1102 12-13 0.6573631968983875 1102 14-15 0.8649089960664966 1102 16-17 1.039860111115054 1102 18-19 1.052589480361334 1102 20-21 0.9630057817941591 1102 22-23 1.0058311803824935 1102 24-25 0.9782479228357133 1102 26-27 1.1244888856426698 1102 28-29 1.0768225152477129 1102 30-31 1.1544632419055603 1102 32-33 1.0313580472563828 1102 34-35 1.124070923753166 1102 36-37 1.0484366907964642 1102 38-39 0.8536089806787928 1102 40-41 1.0148053394447203 1102 42-43 0.8769809908037729 1102 44-45 0.9340113617287784 1102 46-47 0.661520633915778 1102 48-49 0.682216315467624 1102 50-51 0.6644007017100577 1102 52-53 0.803261308764192 1102 54-55 0.9519545489734078 1102 56-57 0.8877674852959245 1102 58-59 0.8754546675613248 1102 60-61 1.0647989375282698 1102 62-63 0.7524329059565993 1102 64-65 0.715895892140864 1102 66-67 0.7862225063697075 1102 68-69 0.46908583665814163 1102 70-71 0.6663203901511849 1102 72-73 0.70098954180947 1102 74-75 0.3342687382361369 1102 76-77 0.335093319974078 1102 78-79 0.24292183911986598 1102 80-81 0.3634137626886229 1102 82-83 0.39015050678475305 1102 84-85 0.19530043523083052 1102 86-87 -0.09192092500989091 1102 88-89 -0.02274806969301224 1102 90-91 0.27779105410142435 1102 92-93 0.4032737538829352 1102 94-95 0.4276960172243811 1102 96-97 -0.27600511850328147 1102 98-99 -0.7854874733487023 1102 100-101 -0.5610654874916765 1103 1 14.810871662272099 1103 2 7.867738959647667 1103 3 3.680962851112998 1103 4 2.1319934738844566 1103 5 1.5637847248057781 1103 6 1.1375793928368978 1103 7 0.8674728090500139 1103 8 0.9504399081471107 1103 9 1.034551847659678 1103 10-11 0.9511544168787154 1103 12-13 0.600660261901389 1103 14-15 0.760791061079864 1103 16-17 0.9915043192092696 1103 18-19 0.8855986041347919 1103 20-21 0.7416668540397566 1103 22-23 0.8710175239665077 1103 24-25 1.0920147304690673 1103 26-27 1.0630490135704562 1103 28-29 1.2004778008763708 1103 30-31 1.0272362758557847 1103 32-33 0.9324985707896545 1103 34-35 1.005644541342825 1103 36-37 1.094732115220964 1103 38-39 0.9941275362230542 1103 40-41 0.9404140231034361 1103 42-43 1.0240153496196527 1103 44-45 1.0970702421117338 1103 46-47 0.8985361603008784 1103 48-49 0.8120899847012772 1103 50-51 0.79541525743684 1103 52-53 0.8673223731283883 1103 54-55 0.7809919068746289 1103 56-57 0.8249102550321297 1103 58-59 0.7752667580648414 1103 60-61 1.1994321510502886 1103 62-63 0.8995519381189929 1103 64-65 0.752513977496573 1103 66-67 0.7005063243754179 1103 68-69 0.5989526615061642 1103 70-71 0.7467334695647274 1103 72-73 0.7003460658571186 1103 74-75 0.5389428832582972 1103 76-77 0.40533807935244326 1103 78-79 0.290462889878242 1103 80-81 0.21311572080513486 1103 82-83 0.02722742523493693 1103 84-85 0.09613104290857066 1103 86-87 0.02508737328917121 1103 88-89 -0.02974090590271672 1103 90-91 0.01740112115566106 1103 92-93 0.11639656704987189 1103 94-95 -0.009327960939952362 1103 96-97 -0.6448652897788456 1103 98-99 -0.733137152803307 1103 100-101 -0.7373644170712446 1104 1 14.955064712249897 1104 2 8.184523165699318 1104 3 3.8731523888467265 1104 4 2.3949882791878636 1104 5 1.9315643653383532 1104 6 1.340956841601738 1104 7 1.094793747320928 1104 8 1.162874924663015 1104 9 1.2217542576484703 1104 10-11 0.9431642109425482 1104 12-13 0.8123752086502734 1104 14-15 1.004064436716753 1104 16-17 1.228428278328657 1104 18-19 1.1503058275306373 1104 20-21 1.256761340174954 1104 22-23 1.2839262155759457 1104 24-25 1.3427918941129633 1104 26-27 1.2710319023314796 1104 28-29 1.2664494022725066 1104 30-31 1.252317362102879 1104 32-33 1.2575129562564555 1104 34-35 1.2694019754932242 1104 36-37 1.3439754098014731 1104 38-39 1.1141941175392702 1104 40-41 1.245142909319874 1104 42-43 1.1005180086910542 1104 44-45 0.8706655933660059 1104 46-47 0.659503610320094 1104 48-49 0.7345960226151966 1104 50-51 0.8676044666443339 1104 52-53 0.8602647336988625 1104 54-55 0.7286519539036611 1104 56-57 0.8097558875247657 1104 58-59 0.7567263304232448 1104 60-61 0.5905804597375166 1104 62-63 0.5771452545016977 1104 64-65 0.43076394812831964 1104 66-67 0.4860537585855518 1104 68-69 0.5407933641142684 1104 70-71 0.4998264444792575 1104 72-73 0.45519431660426335 1104 74-75 0.2738496415406466 1104 76-77 0.2871538537883218 1104 78-79 0.1375070995536376 1104 80-81 0.020250414568906194 1104 82-83 0.008468527649508673 1104 84-85 0.01616083924222167 1104 86-87 -0.03996639550402037 1104 88-89 -0.1917368421327872 1104 90-91 -0.07308590404926818 1104 92-93 0.27352225123064144 1104 94-95 0.34166289408440775 1104 96-97 -0.14364391884883432 1104 98-99 -0.4708685895779361 1104 100-101 -0.3401045249473107 1105 1 14.835224532127427 1105 2 8.056524412543418 1105 3 3.8713610356356725 1105 4 2.3202320152395544 1105 5 1.714555760675708 1105 6 1.4154479935503232 1105 7 1.0481743292412773 1105 8 1.1363898319669161 1105 9 1.1124196454198412 1105 10-11 1.0364076281899202 1105 12-13 0.8546924924453236 1105 14-15 1.0257685482777745 1105 16-17 1.2489618480001567 1105 18-19 1.0158947332978556 1105 20-21 0.9831534417091987 1105 22-23 1.0174892469636845 1105 24-25 1.209968614855839 1105 26-27 1.2190801962603928 1105 28-29 1.4271142196245208 1105 30-31 1.1875515153064882 1105 32-33 0.7748083862910704 1105 34-35 1.1893266742275728 1105 36-37 1.1070973947189557 1105 38-39 1.1431821591260913 1105 40-41 1.3824451134537483 1105 42-43 1.2979909819955324 1105 44-45 1.2539481390165506 1105 46-47 1.0195041304952568 1105 48-49 1.0038199675782984 1105 50-51 1.106748691808452 1105 52-53 1.1943691511780514 1105 54-55 1.1996494794264798 1105 56-57 1.2926903000376626 1105 58-59 1.1631461785734736 1105 60-61 0.9567054020675272 1105 62-63 1.1121107293531516 1105 64-65 1.2138178531512303 1105 66-67 1.1878646282169463 1105 68-69 1.1452746337199677 1105 70-71 1.1783293291362291 1105 72-73 1.1066720327633988 1105 74-75 1.0839914217829936 1105 76-77 0.9561943286373129 1105 78-79 0.9982484752612812 1105 80-81 0.8486517222183281 1105 82-83 0.9700136429436732 1105 84-85 0.9493485268143793 1105 86-87 0.9188945962552388 1105 88-89 0.8578812182699913 1105 90-91 1.2222308348426019 1105 92-93 1.458035966762015 1105 94-95 1.3030618086325347 1105 96-97 0.7752628916725328 1105 98-99 0.41615163134478905 1105 100-101 0.40738196010400607 1106 1 14.55579372939092 1106 2 7.7357528804983815 1106 3 3.565440940649289 1106 4 2.158126744069989 1106 5 1.5851593430348458 1106 6 1.176584693608639 1106 7 0.8981471546506157 1106 8 0.9350152493374821 1106 9 1.1013737526586809 1106 10-11 0.8927106761004211 1106 12-13 0.7121354510917826 1106 14-15 0.8590800998773034 1106 16-17 0.9781283206341413 1106 18-19 0.8810103702693581 1106 20-21 0.866924758140108 1106 22-23 0.8716894812850668 1106 24-25 0.964686863872835 1106 26-27 0.8059268731839992 1106 28-29 1.0376851604281825 1106 30-31 0.8473271924905745 1106 32-33 0.863091856478789 1106 34-35 0.8867652224484743 1106 36-37 0.9548022659458439 1106 38-39 1.131975532191312 1106 40-41 1.1165505316374933 1106 42-43 0.7639001150114169 1106 44-45 0.7539865449701821 1106 46-47 0.8242838140161766 1106 48-49 0.6546771013364712 1106 50-51 0.6690373401402105 1106 52-53 0.6469589292256899 1106 54-55 0.6131293282181822 1106 56-57 0.5465534031926467 1106 58-59 0.6511147499851653 1106 60-61 0.6483907184048974 1106 62-63 0.6878509753174953 1106 64-65 0.8639574915904724 1106 66-67 0.8140460619477494 1106 68-69 0.6486860037449595 1106 70-71 0.8211735388653842 1106 72-73 0.5475926600252556 1106 74-75 0.46424040652886944 1106 76-77 0.49726851962616436 1106 78-79 0.4631393748128403 1106 80-81 0.5221680181098165 1106 82-83 0.5513554998950632 1106 84-85 0.4165450354077471 1106 86-87 0.6384358459018493 1106 88-89 0.6255101268268675 1106 90-91 0.801899804564588 1106 92-93 1.0099137475007716 1106 94-95 0.8840961675900978 1106 96-97 0.49534007211672204 1106 98-99 0.09666567225098532 1106 100-101 -0.06887820332809724 1107 1 14.70399212125491 1107 2 7.939667871723973 1107 3 3.744042180882868 1107 4 2.2618221555091367 1107 5 1.5692899579780075 1107 6 1.2002582328970703 1107 7 0.9941963951332937 1107 8 0.9268926335575998 1107 9 0.821015215454068 1107 10-11 0.866777716497829 1107 12-13 0.6891942935244728 1107 14-15 0.7828381996388956 1107 16-17 1.0439698714929833 1107 18-19 0.7857994491240703 1107 20-21 0.7084746189791034 1107 22-23 0.7925019364748991 1107 24-25 0.8969844436848291 1107 26-27 0.9457801453439743 1107 28-29 1.0757886705111588 1107 30-31 0.943036215042163 1107 32-33 0.9818700299965215 1107 34-35 0.8776392681616301 1107 36-37 0.8291270196795608 1107 38-39 0.7530577365879338 1107 40-41 1.0711467072246776 1107 42-43 1.0325788678477394 1107 44-45 0.785574667102928 1107 46-47 0.9359969515181135 1107 48-49 0.9279753430215401 1107 50-51 1.0136359388694807 1107 52-53 1.0736537913660698 1107 54-55 1.1012496446078792 1107 56-57 0.9509638910465945 1107 58-59 1.1433536095195684 1107 60-61 1.2459995821548517 1107 62-63 1.0201859601399228 1107 64-65 0.9307875107680275 1107 66-67 0.9038722783491089 1107 68-69 1.0247188331286097 1107 70-71 1.1264251204945346 1107 72-73 1.1443985336098947 1107 74-75 1.0805840108708828 1107 76-77 0.8563397037162979 1107 78-79 0.8089108222150863 1107 80-81 0.8550465133026535 1107 82-83 0.9173910218805865 1107 84-85 0.9067504216494093 1107 86-87 1.08153641787516 1107 88-89 1.235931204650047 1107 90-91 1.592484739886256 1107 92-93 1.59374029204427 1107 94-95 1.5062029179769247 1107 96-97 0.9286027670658861 1107 98-99 0.5214454737050289 1107 100-101 0.5200812507583485 1108 1 14.29880687867783 1108 2 7.5112780372857095 1108 3 3.362345845468486 1108 4 -0.019529330465800854 1108 5 0.42683862101018377 1108 6 0.5508243130672241 1108 7 0.4853956627726319 1108 8 0.5710799072066806 1108 9 0.708323759095066 1108 10-11 0.690558020277571 1108 12-13 0.38095549688053154 1108 14-15 0.31297422079358483 1108 16-17 0.6522037218428522 1108 18-19 0.5112633187644917 1108 20-21 0.5410786959619927 1108 22-23 -0.184911371992996 1108 24-25 0.27508858133709424 1108 26-27 0.28616125536964887 1108 28-29 0.24643127526053377 1108 30-31 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0.20719507585098995 2312 12-13 -0.5374150218493909 2312 14-15 0.29309554841284324 2312 16-17 -0.22978201765199202 2312 18-19 0.0031605025113066176 2312 20-21 0.1457710636082652 2312 22-23 0.2892005611773598 2312 24-25 0.3338558779236891 2312 26-27 0.2909878096051344 2312 28-29 -0.4879458857286778 2312 30-31 0.09021506039675842 2312 32-33 0.3695236174828267 2312 34-35 -0.7034615838427527 2312 36-37 0.11363829787504898 2312 38-39 0.39822695674889275 2312 40-41 0.3068069161641134 2312 42-43 0.2537129215750227 2312 44-45 0.42877322568531895 2312 46-47 0.216802766306337 2312 48-49 0.3760859619054351 2312 50-51 0.2811685738488876 2312 52-53 0.19054491994127432 2312 54-55 0.08590043615962628 2312 56-57 0.17700617955681608 2312 58-59 0.23751473469431517 2312 60-61 0.43822627010588633 2312 62-63 0.2570281126317049 2312 64-65 0.2818526107037975 2312 66-67 0.1905930060477523 2312 68-69 0.6501235824390719 2312 70-71 0.5173730462133541 2312 72-73 0.1890348259739696 2312 74-75 0.4004076119590323 2312 76-77 0.3131336830112694 2312 78-79 0.4001250229178481 2312 80-81 0.5072812301198191 2312 82-83 0.39767831360235206 2312 84-85 0.4861600166167257 2312 86-87 0.45069055321350504 2312 88-89 0.7896951843228486 2312 90-91 1.0977753848403502 2312 92-93 1.468522662543382 2312 94-95 1.7140473563341239 2312 96-97 1.415279099857667 2312 98-99 1.1061133620187817 2312 100-101 0.84802038250732 2313 1 -14.150593305880768 2313 2 -7.29677117023831 2313 3 -3.7392722323987435 2313 4 -1.0963589588324005 2313 5 -0.584740069441338 2313 6 -1.0624596892488256 2313 7 -0.33990884372185803 2313 8 -0.09714300783403473 2313 9 -2.4626048829427347 2313 10-11 -0.6747451383480794 2313 12-13 -0.7987583682429147 2313 14-15 -0.9819961016941647 2313 16-17 -0.8531079769681256 2313 18-19 -0.22338388899724748 2313 20-21 -1.1569515465687843 2313 22-23 -2.347029058066795 2313 24-25 -1.5087190118598812 2313 26-27 -0.39634460403897975 2313 28-29 0.003744729615426934 2313 30-31 -1.2904808727914983 2313 32-33 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1.180485591093241 2313 98-99 0.7257198010436099 2313 100-101 0.8324371412554328 2314 1 -14.150593305880768 2314 2 -7.287385446225292 2314 3 -3.605746983974651 2314 4 -1.4273056967430584 2314 5 -1.3542469106551849 2314 6 -6.387779007686511 2314 7 -2.0400598559104424 2314 8 -4.35944521125559 2314 9 -1.7572025349292701 2314 10-11 -0.5318545118645233 2314 12-13 -3.061721634301975 2314 14-15 -2.8813905156900077 2314 16-17 -2.643132084662419 2314 18-19 -1.4783487085828924 2314 20-21 -2.023385967943419 2314 22-23 -1.2389310972738983 2314 24-25 -3.0439643524104554 2314 26-27 -4.680855772158949 2314 28-29 -2.031867245018148 2314 30-31 -1.9939791108319227 2314 32-33 -4.216266991971324 2314 34-35 -2.867197358324276 2314 36-37 -1.2349213186890182 2314 38-39 -1.8442497031788747 2314 40-41 -2.7873256963338946 2314 42-43 -1.6769578641506264 2314 44-45 -2.695262392059952 2314 46-47 -2.3347941262271092 2314 48-49 -1.3830251845407417 2314 50-51 -2.457904637881345 2314 52-53 -1.1668916473076614 2314 54-55 -0.6954561274548503 2314 56-57 -1.2586086127108622 2314 58-59 -1.7628680197749382 2314 60-61 -0.8185504148088185 2314 62-63 -2.984107884460723 2314 64-65 -1.3565638988480266 2314 66-67 -2.394632898593006 2314 68-69 -2.305175920093692 2314 70-71 -2.822852517696422 2314 72-73 -2.959776704362447 2314 74-75 -2.3707281579794106 2314 76-77 -1.289531741880264 2314 78-79 -0.7785188677756523 2314 80-81 -0.5092455854084257 2314 82-83 -0.6076548077205892 2314 84-85 -0.32556589938172564 2314 86-87 -0.14622037970792334 2314 88-89 -0.08918199351601075 2314 90-91 0.44120132128266576 2314 92-93 0.8958638251174946 2314 94-95 0.9226519493877525 2314 96-97 0.8226559256078438 2314 98-99 0.8119087417066702 2314 100-101 1.0213295617999627 2315 1 -14.150593305880768 2315 2 -7.380470945081807 2315 3 -4.718542995912408 2315 4 -3.032531218285719 2315 5 -3.4924372686777474 2315 6 -2.93246263144896 2315 7 -3.3745038802411464 2315 8 -3.833638072829743 2315 9 -2.6149075636907497 2315 10-11 -5.07365519685386 2315 12-13 -4.76390370711054 2315 14-15 -3.5219735642229324 2315 16-17 -4.581305464787452 2315 18-19 -1.742450091409708 2315 20-21 -3.481781033523994 2315 22-23 -4.42068848403591 2315 24-25 -5.064773197803294 2315 26-27 -4.861674694163405 2315 28-29 -2.70918991317275 2315 30-31 -3.4596336500793115 2315 32-33 -4.595612232470124 2315 34-35 -3.9466948492663363 2315 36-37 -5.211273952373901 2315 38-39 -3.5074884252662955 2315 40-41 -4.693033206621585 2315 42-43 -3.2015558091826186 2315 44-45 -1.8571706893879067 2315 46-47 -1.2688681241640651 2315 48-49 -1.0094691317826125 2315 50-51 -0.8881300676515842 2315 52-53 -0.5653946556075589 2315 54-55 -2.0572765068053194 2315 56-57 -4.07622799945085 2315 58-59 -3.5875117592440553 2315 60-61 -4.796417660498268 2315 62-63 -2.8016451348856286 2315 64-65 -3.6465340911663127 2315 66-67 -2.2028502009978084 2315 68-69 -1.5114168116811015 2315 70-71 -2.1551732953391927 2315 72-73 -2.2845816879718903 2315 74-75 -2.754516651920593 2315 76-77 -2.570344331587801 2315 78-79 -1.8819785044302932 2315 80-81 -1.5570712214830635 2315 82-83 -1.0076180987529568 2315 84-85 -0.9939092787358703 2315 86-87 -1.7033208117724712 2315 88-89 -2.558993639803081 2315 90-91 -2.122993852956448 2315 92-93 -1.0080574268051379 2315 94-95 -0.7345678785940244 2315 96-97 -1.6128442157216831 2315 98-99 -1.1047001389267805 2315 100-101 -0.7212270526053715 2316 1 -14.150593305880768 2316 2 -7.386159431815326 2316 3 -4.986970369700899 2316 4 -2.803488903130578 2316 5 -2.1275495024821858 2316 6 -1.2999796847104363 2316 7 -4.66852491601631 2316 8 -2.9371078334870866 2316 9 -1.4156887256081063 2316 10-11 -4.44719407621994 2316 12-13 -3.817635805021194 2316 14-15 -1.9862038561679682 2316 16-17 -3.310284429751608 2316 18-19 -2.5277639183674765 2316 20-21 -3.3307642051995963 2316 22-23 -2.9873920764861595 2316 24-25 -3.59560824481089 2316 26-27 -4.0525368981179355 2316 28-29 -3.840890385706949 2316 30-31 -2.7749090214842482 2316 32-33 -2.9372554689616095 2316 34-35 -4.271470623783646 2316 36-37 -2.6517065671777544 2316 38-39 -3.4994956735262193 2316 40-41 -3.218139843799378 2316 42-43 -6.923272605827304 2316 44-45 -3.771768301543162 2316 46-47 -5.000646927291818 2316 48-49 -4.528933812965267 2316 50-51 -3.472287030479851 2316 52-53 -4.779516360427273 2316 54-55 -4.863679293511371 2316 56-57 -5.009519093162552 2316 58-59 -3.6823948658967574 2316 60-61 -3.392194000223114 2316 62-63 -2.3225902975556103 2316 64-65 -2.5103495710511687 2316 66-67 -2.923162480935602 2316 68-69 -3.0666307310904166 2316 70-71 -3.335858298043245 2316 72-73 -4.007446550878566 2316 74-75 -4.616780444888288 2316 76-77 -4.406981054700058 2316 78-79 -4.528316891115793 2316 80-81 -4.4820959701061795 2316 82-83 -4.042425124182859 2316 84-85 -3.6636196348240126 2316 86-87 -3.8856639167764975 2316 88-89 -3.6118598593557145 2316 90-91 -2.8003157968711534 2316 92-93 -2.7411118283080214 2316 94-95 -1.987204327789481 2316 96-97 -2.096416323672255 2316 98-99 -2.520274573137028 2316 100-101 -2.4141978799117325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20444.0 3 6116.0 4 6982.0 5 8338.0 6 9968.0 7 12021.0 8 14813.0 9 18002.0 10 21391.0 11 25321.0 12 28311.0 13 31393.0 14 33935.0 15 37477.0 16 41497.0 17 43578.0 18 42614.0 19 43572.0 20 47054.0 21 52179.0 22 57721.0 23 63257.0 24 70422.0 25 78858.0 26 88663.0 27 99117.0 28 111442.0 29 125892.0 30 142997.0 31 161770.0 32 185272.0 33 212765.0 34 245254.0 35 284744.0 36 295785.0 37 172663.0 38 21866.0 39 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.160660790165196 19.146857911714477 13.81682220420555 23.875659093914773 2 15.335148512845176 20.92412045298883 40.120217929614626 23.620513104551367 3 18.810949633550518 25.114238861415405 30.532468295886588 25.54234320914749 4 12.638426130187758 17.080943469904202 38.36250192287862 31.918128477029416 5 13.476199710668052 37.38723520417092 35.17927161594123 13.957293469219797 6 30.96136832593376 37.01000750884198 18.224452871709644 13.804171293514617 7 26.823532115409115 32.93312636935365 22.603481144952852 17.63986037028438 8 25.07134147660297 35.07885074998107 21.06280212828083 18.787005645135128 9 25.981808898481056 16.554192985710756 21.024519161075084 36.439478954733104 10-11 24.275710960121362 26.34236906744872 28.917553290333913 20.464366682096003 12-13 25.333048587611096 24.096854545340886 28.662394016555236 21.907702850492782 14-15 22.6066131054676 25.613453369264587 26.66482943947618 25.11510408579164 16-17 21.955842784430505 28.279101987370375 27.483596274472532 22.28145895372658 18-19 21.843391524905076 27.547497039223508 29.17178856921458 21.43732286665683 20-21 22.825594415640936 26.815419524129453 28.8398848986525 21.519101161577108 22-23 22.337769123969245 26.68143366439335 28.864397814331067 22.11639939730634 24-25 22.429507349579154 26.745339043810688 28.969591166977356 21.855562439632806 26-27 22.16405701002403 26.78929043175496 29.135664946734867 21.910987611486142 28-29 22.07090738964132 27.261423402847267 28.9176278670735 21.750041340437914 30-31 22.200343424224293 26.933452340165175 28.83877358927694 22.027430646333592 32-33 22.106214340765572 26.932197445842604 28.849607860444245 22.111980352947583 34-35 22.497769163636708 26.97261219357066 28.7009313213483 21.82868732144433 36-37 22.479878721244813 26.65968854025304 28.952936188685293 21.90749654981686 38-39 22.14768265855561 27.125256555497973 28.828994667567294 21.898066118379127 40-41 22.483734772452472 26.986729359359053 28.716846852374832 21.812689015813643 42-43 22.188354905366175 27.115094507054753 28.374563171617034 22.321987415962035 44-45 22.388689996498886 27.042670489998834 28.370642773207393 22.197996740294887 46-47 22.578151765244215 27.058758276986637 28.114850873042634 22.248239084726514 48-49 22.41352020454822 27.155112352710397 28.19780773549426 22.23355970724712 50-51 22.14335412542158 27.38507779528774 28.30108648554853 22.170481593742146 52-53 22.489432760961662 27.44000619840932 27.770768477569845 22.299792563059174 54-55 22.394145664550596 27.295226725425625 27.790739036441252 22.519888573582524 56-57 22.279962465272003 27.488506300121905 27.712047284693202 22.519483949912885 58-59 22.422680673760365 27.248721697754412 27.820184075523365 22.50841355296186 60-61 22.311091771798818 27.225745222817956 28.19279536235177 22.27036764303146 62-63 22.14181828202341 27.193927845747506 28.28291434867145 22.381339523557628 64-65 22.117865892970094 27.469597734354632 27.893628666891374 22.518907705783896 66-67 22.05713026024595 27.481260847948423 27.643738116599202 22.81787077520642 68-69 22.183693898269674 27.326954692651817 27.9551838625935 22.534167546485005 70-71 22.291400506935492 27.051160655070344 27.81846143910781 22.83897739888635 72-73 22.2479919011513 27.043671658442857 27.897994006351446 22.810342434054395 74-75 22.04398296187531 27.5652710611515 27.577297136931335 22.813448840041858 76-77 22.162799882613722 27.27789848116703 27.597919861030885 22.961381775188364 78-79 22.21014216375835 27.388811358893257 27.586006849663768 22.81503962768463 80-81 22.295762538562855 27.1495530945767 27.551575996418194 23.003108370442252 82-83 22.212059490541584 27.13218780480091 27.773488496146665 22.88226420851084 84-85 22.18749931312213 27.238794929632427 27.52462066165825 23.049085095587195 86-87 22.006618824401954 27.096647315683022 27.74877281530348 23.147961044611538 88-89 22.13989461984224 27.002046287250973 27.52696859817402 23.331090494732766 90-91 21.951146519355625 26.997525860280515 27.728236557041747 23.323091063322117 92-93 22.070329671816054 27.022203781629113 27.71906883571325 23.188397710841578 94-95 22.085779162843405 26.921717951211676 27.60893226380708 23.38357062213784 96-97 21.971182632767636 26.737537883639256 27.758917850555946 23.532361633037155 98-99 21.86783290235207 26.94284665590299 27.407429845044735 23.781890596700197 100-101 22.01600789480497 26.665847675410077 27.551931914075634 23.766212515709316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3608.0 1 2401.0 2 1075.0 3 859.5 4 768.5 5 775.0 6 910.0 7 1730.5 8 2383.5 9 2230.0 10 2020.5 11 2007.5 12 2136.0 13 2210.0 14 2380.5 15 2691.0 16 2913.0 17 3184.5 18 3523.0 19 4009.0 20 4676.5 21 5613.0 22 6984.5 23 8641.5 24 10714.0 25 13819.0 26 18739.0 27 24389.5 28 29737.0 29 34878.5 30 40090.0 31 46648.5 32 52502.0 33 57365.0 34 63841.0 35 70623.0 36 77071.0 37 81701.0 38 85060.0 39 87815.0 40 89945.0 41 93122.5 42 95558.5 43 98358.0 44 101942.5 45 104116.0 46 106063.0 47 111960.0 48 115982.5 49 112533.0 50 108002.0 51 105275.0 52 103382.0 53 100670.5 54 95156.5 55 87530.5 56 79734.0 57 72504.0 58 65241.0 59 56934.0 60 47701.0 61 38743.0 62 30377.0 63 22693.0 64 16768.0 65 12103.5 66 8627.0 67 6284.5 68 4446.0 69 3119.5 70 2120.5 71 1320.0 72 787.5 73 515.5 74 346.0 75 216.0 76 137.0 77 96.5 78 61.0 79 38.0 80 31.0 81 24.0 82 21.0 83 18.0 84 14.0 85 12.5 86 11.5 87 6.5 88 5.0 89 7.5 90 6.5 91 3.5 92 3.5 93 6.0 94 4.5 95 3.0 96 3.5 97 2.0 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.05388445680771 2 0.08985635467986944 3 0.12123690663341004 4 0.19617906350310213 5 0.12474612964756941 6 0.14985057121040188 7 0.15690275976770293 8 0.15558680113739318 9 0.14374317346460527 10-11 0.13122469521217134 12-13 0.1435238470262203 14-15 0.15013738270675145 16-17 0.1453122010622823 18-19 0.15229690456161876 20-21 0.1807418564936991 22-23 0.1989965646731243 24-25 0.22658108211615594 26-27 0.22017000160951863 28-29 0.21817919239956285 30-31 0.23370075573142163 32-33 0.22391542232655412 34-35 0.20947361992212898 36-37 0.1958753807422614 38-39 0.1917250496774383 40-41 0.19228180140564627 42-43 0.18580323584104433 44-45 0.20210087733949608 46-47 0.22863937638407636 48-49 0.2314400062896074 50-51 0.2431486638464661 52-53 0.2706825674960243 54-55 0.2560214386532142 56-57 0.23250289595254992 58-59 0.18875570712699574 60-61 0.19951957387234998 62-63 0.20422665666538106 64-65 0.19226493014115512 66-67 0.20159473940476155 68-69 0.1897004979385002 70-71 0.20392297390454034 72-73 0.224573401641709 74-75 0.2545030248490106 76-77 0.25531284554458583 78-79 0.25021772366825834 80-81 0.2553803306025505 82-83 0.2457637098425945 84-85 0.2158678291642752 86-87 0.22774519936604537 88-89 0.22946606834414277 90-91 0.2169475900917088 92-93 0.2151086222621734 94-95 0.25244473058109024 96-97 0.28183447332467504 98-99 0.30123642748949847 100-101 0.2957363952653833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2963619.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.10703222798706 #Duplication Level Percentage of deduplicated Percentage of total 1 78.88607070081022 52.14924018157727 2 12.048065886442535 15.929237596799364 3 4.13861401970866 8.207744711402384 4 1.8595213680643292 4.917097560290369 5 1.0314720900600571 3.4093779349934685 6 0.5856574002160996 2.3229643578386887 7 0.36868026672960447 1.706065079216178 8 0.24211760826287881 1.280454122591782 9 0.1724710673340557 1.0261395365982973 >10 0.6404552276409747 6.729924602343258 >50 0.018844428677457267 0.8782834877303657 >100 0.007466863025118203 0.9092450866881516 >500 2.8153651411300806E-4 0.12224061711923416 >1k 2.815365140084714E-4 0.41198512481115757 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3516 0.11863873190177279 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.3742528982301704E-5 2 3.3742528982301704E-5 0.0 0.0 0.0 6.748505796460341E-5 3 3.3742528982301704E-5 0.0 0.0 0.0 6.748505796460341E-5 4 3.3742528982301704E-5 0.0 0.0 0.0 6.748505796460341E-5 5 3.3742528982301704E-5 0.0 0.0 0.0 6.748505796460341E-5 6 3.3742528982301704E-5 0.0 0.0 0.0 1.3497011592920682E-4 7 3.3742528982301704E-5 0.0 0.0 0.0 1.3497011592920682E-4 8 3.3742528982301704E-5 0.0 0.0 0.0 2.0245517389381023E-4 9 3.3742528982301704E-5 0.0 0.0 0.0 2.3619770287611194E-4 10-11 1.5184138042035768E-4 1.6871264491150852E-5 0.0 5.0613793473452556E-5 2.3619770287611194E-4 12-13 2.3619770287611194E-4 3.3742528982301704E-5 0.0 1.5184138042035768E-4 2.530689673672628E-4 14-15 2.3619770287611194E-4 3.3742528982301704E-5 0.0 1.8558390940265937E-4 4.5552414126107305E-4 16-17 2.3619770287611194E-4 3.3742528982301704E-5 0.0 2.530689673672628E-4 5.73622992699129E-4 18-19 2.3619770287611194E-4 3.3742528982301704E-5 0.0 2.868114963495645E-4 5.73622992699129E-4 20-21 3.3742528982301707E-4 3.3742528982301704E-5 0.0 5.061379347345256E-4 5.904942571902799E-4 22-23 3.3742528982301707E-4 3.3742528982301704E-5 0.0 9.447908115044479E-4 7.929494310840901E-4 24-25 3.3742528982301707E-4 5.0613793473452556E-5 0.0 0.001265344836836314 9.447908115044478E-4 26-27 3.711678188053188E-4 6.748505796460341E-5 0.0 0.0021426505903761584 0.0010122758694690512 28-29 4.723954057522239E-4 6.748505796460341E-5 1.6871264491150852E-5 0.004808310379977993 0.001029147133960202 30-31 5.061379347345256E-4 6.748505796460341E-5 3.3742528982301704E-5 0.012518478252433934 0.0010797609274336545 32-33 5.061379347345256E-4 6.748505796460341E-5 3.3742528982301704E-5 0.02922103009867328 0.0012147310433628614 34-35 5.061379347345256E-4 6.748505796460341E-5 3.3742528982301704E-5 0.05538836132444826 0.0012822161013274647 36-37 5.061379347345256E-4 8.435632245575427E-5 3.3742528982301704E-5 0.09291005355276774 0.0012822161013274647 38-39 5.398804637168273E-4 1.0122758694690511E-4 3.3742528982301704E-5 0.14672938727953896 0.0013328298948009174 40-41 5.73622992699129E-4 1.0122758694690511E-4 3.3742528982301704E-5 0.2054245164442528 0.00138344368827437 42-43 5.73622992699129E-4 1.3497011592920682E-4 3.3742528982301704E-5 0.2696028065685906 0.0014003149527655207 44-45 6.073655216814307E-4 1.3497011592920682E-4 3.3742528982301704E-5 0.34847596806472086 0.0014678000107301243 46-47 6.411080506637324E-4 1.3497011592920682E-4 3.3742528982301704E-5 0.44206087219713464 0.0016365126556416327 48-49 6.411080506637324E-4 1.3497011592920682E-4 3.3742528982301704E-5 0.5396442660139511 0.0017546115070796887 50-51 6.411080506637324E-4 1.3497011592920682E-4 3.3742528982301704E-5 0.6427951771128475 0.0017546115070796887 52-53 6.748505796460341E-4 1.3497011592920682E-4 3.3742528982301704E-5 0.748426164091943 0.0018895816230088955 54-55 6.748505796460341E-4 1.3497011592920682E-4 3.3742528982301704E-5 0.8694268730224769 0.0020245517389381024 56-57 6.748505796460341E-4 1.3497011592920682E-4 3.3742528982301704E-5 1.014941529258653 0.002075165532411555 58-59 7.085931086283358E-4 1.3497011592920682E-4 3.3742528982301704E-5 1.168267580954232 0.002159521854867309 60-61 7.085931086283358E-4 1.3497011592920682E-4 3.3742528982301704E-5 1.332559954569059 0.002193264383849611 62-63 7.085931086283358E-4 1.3497011592920682E-4 3.3742528982301704E-5 1.505372316751917 0.0022607494418142144 64-65 7.085931086283358E-4 1.3497011592920682E-4 3.3742528982301704E-5 1.6895052974083375 0.002277620706305365 66-67 7.085931086283358E-4 1.6871264491150853E-4 3.3742528982301704E-5 1.905406869101595 0.002328234499778818 68-69 7.423356376106376E-4 1.6871264491150853E-4 3.3742528982301704E-5 2.1471383467308045 0.002328234499778818 70-71 7.423356376106376E-4 1.6871264491150853E-4 3.3742528982301704E-5 2.4111567647528243 0.0024632046157080246 72-73 7.423356376106376E-4 1.6871264491150853E-4 3.3742528982301704E-5 2.6859896633136717 0.0029018574924779468 74-75 7.592069021017884E-4 1.6871264491150853E-4 3.3742528982301704E-5 2.9709621918337006 0.0029018574924779468 76-77 7.760781665929392E-4 1.6871264491150853E-4 3.3742528982301704E-5 3.2809379343296152 0.0029018574924779468 78-79 7.760781665929392E-4 1.6871264491150853E-4 6.748505796460341E-5 3.6343909254192255 0.0029018574924779468 80-81 8.098206955752409E-4 1.6871264491150853E-4 6.748505796460341E-5 4.014686098314257 0.0030199563439160027 82-83 8.098206955752409E-4 1.8558390940265937E-4 6.748505796460341E-5 4.410941487417917 0.0032055402533186623 84-85 8.098206955752409E-4 2.0245517389381023E-4 6.748505796460341E-5 4.820019037534852 0.003222411517809813 86-87 8.098206955752409E-4 2.0245517389381023E-4 6.748505796460341E-5 5.250978617696809 0.0032392827823009636 88-89 8.098206955752409E-4 2.0245517389381023E-4 6.748505796460341E-5 5.7147528072940545 0.0033742528982301707 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6635 0.0 27.596428 1 GTATCAA 14240 0.0 27.412216 1 TACATGG 7160 0.0 22.631443 2 GAGTACT 7315 0.0 22.002441 12-13 TATACCG 215 4.737194E-8 21.952368 5 TATCAAC 17505 0.0 21.887182 2 ACATGGG 7115 0.0 21.778975 3 ATCAACG 18160 0.0 20.942017 3 CAACGCA 18235 0.0 20.784023 5 TCAACGC 18445 0.0 20.6423 4 AACGCAG 18705 0.0 20.438066 6 GTGTAGC 1495 0.0 20.188427 1 GGTATCA 11020 0.0 20.175909 1 AGAGTAC 11170 0.0 19.562723 10-11 ACGCAGA 19120 0.0 19.327976 7 AGTACTT 8190 0.0 19.21953 12-13 GTACTTT 8370 0.0 19.03547 14-15 CGCAGAG 19525 0.0 18.395267 8 CATGGGG 4515 0.0 18.10048 4 ACCGTGC 625 0.0 17.36842 8 >>END_MODULE