FastQCFastQC Report
Fri 27 May 2016
SRR939674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963619
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87310.29460602054447615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70130.23663635575288186No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50180.16932001043318995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45830.15464201032588873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143750.023.9268421
GGTATCA107750.021.7623021
GTACATG73200.020.8188691
ATCAACG163700.020.5726643
TCAACGC164000.020.5350324
TACATGG70250.020.3454192
CAACGCA165350.020.3388485
ACATGGG68100.020.2937153
GAGTACT78100.020.27919812-13
AACGCAG167700.020.0409816
TATCAAC170100.019.8831522
AGAGTAC115050.019.76847610-11
AGTACTT82350.019.63328712-13
CGCAGAG177700.018.1969748
ACGCAGA180700.018.1818127
GTACTTT87150.018.01106514-15
CATGGGG43700.017.0536234
GCAGAGT187650.016.7548229
TCTATAC10300.016.485663
TACTTTT103350.015.50711414-15
Produced by FastQC (version 0.11.5)