##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939674_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2963619 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.501281035112815 31.0 31.0 34.0 30.0 34.0 2 31.709296302932326 31.0 31.0 34.0 30.0 34.0 3 31.764036470275027 31.0 31.0 34.0 30.0 34.0 4 35.56243160811157 37.0 35.0 37.0 33.0 37.0 5 35.31310401235786 37.0 35.0 37.0 33.0 37.0 6 35.3113521002531 37.0 35.0 37.0 32.0 37.0 7 35.22058638441716 37.0 35.0 37.0 32.0 37.0 8 35.18469209436166 37.0 35.0 37.0 32.0 37.0 9 36.78779795918436 39.0 37.0 39.0 32.0 39.0 10-11 36.67107782748052 39.0 35.0 39.0 32.0 39.0 12-13 36.6112921397791 39.0 35.0 39.0 32.0 39.0 14-15 37.698560948623964 39.0 36.5 41.0 32.0 41.0 16-17 37.65069852096373 39.0 36.0 41.0 32.0 41.0 18-19 37.619175744250526 39.0 36.0 41.0 32.0 41.0 20-21 37.55071080324427 39.0 36.0 41.0 32.0 41.0 22-23 37.416663882908026 39.0 36.0 41.0 32.0 41.0 24-25 37.29039343451368 39.0 36.0 41.0 31.0 41.0 26-27 37.0080202617138 39.0 36.0 40.0 31.0 41.0 28-29 36.817068422088 39.0 36.0 40.0 30.0 41.0 30-31 36.592514084975164 38.5 35.0 40.0 30.0 41.0 32-33 36.42234561865071 38.0 35.0 40.0 30.0 41.0 34-35 36.39415609766303 38.0 35.0 40.0 30.0 41.0 36-37 36.323196908914404 38.0 35.0 40.0 30.0 41.0 38-39 36.36559186589099 38.0 35.0 40.0 30.0 41.0 40-41 36.271862206309244 38.0 35.0 40.0 30.0 41.0 42-43 36.106163444086434 38.0 35.0 40.0 29.5 41.0 44-45 35.90445077454288 38.0 35.0 40.0 29.0 41.0 46-47 35.63490583641149 38.0 34.5 40.0 28.0 41.0 48-49 35.365629320098165 38.0 34.0 40.0 27.0 41.0 50-51 35.13705506679502 38.0 34.0 40.0 26.5 41.0 52-53 34.92176625942808 38.0 34.0 40.0 26.0 41.0 54-55 34.64055366091255 38.0 33.0 40.0 26.0 41.0 56-57 33.848704236273285 37.5 32.5 40.0 22.5 41.0 58-59 30.76019218394807 35.5 27.5 39.0 8.5 40.5 60-61 30.49718975347371 34.5 26.5 39.0 10.5 40.0 62-63 32.30964843996479 35.5 30.5 39.0 20.0 40.0 64-65 32.36683207254374 36.0 31.0 39.0 19.0 40.0 66-67 27.473730935049343 32.0 19.0 37.0 4.5 39.5 68-69 19.91282162113281 20.5 8.0 32.5 2.0 37.0 70-71 17.958057867762353 16.0 7.5 29.0 2.0 35.0 72-73 19.70658272875157 20.0 7.5 31.0 2.0 35.5 74-75 21.15855125102113 24.0 9.5 32.5 2.0 36.0 76-77 22.99126254083268 27.5 12.0 33.0 2.0 35.5 78-79 25.20623332486396 30.5 14.0 34.5 2.0 36.5 80-81 26.832777593880994 32.5 23.0 35.0 2.0 37.0 82-83 27.982299006721174 33.0 27.0 35.0 2.0 36.0 84-85 28.029742689596738 34.0 28.0 35.0 2.0 36.0 86-87 27.759762978979417 34.0 27.0 35.0 2.0 36.0 88-89 27.32719877285171 33.0 26.5 35.0 2.0 35.5 90-91 25.505584219833928 32.5 16.0 34.5 2.0 35.0 92-93 23.893438056646282 31.5 4.5 34.0 2.0 35.0 94-95 20.090496450454665 28.5 2.0 34.0 2.0 35.0 96-97 17.361476627056312 19.0 2.0 33.0 2.0 35.0 98-99 17.64274894984814 22.0 2.0 33.0 2.0 35.0 100-101 16.869060597870373 18.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24841981897462873 1101 2 0.23202076908001246 1101 3 0.19042225053086526 1101 4 0.12596285382430494 1101 5 0.301009180338248 1101 6 0.21970184222233513 1101 7 0.23752179144094043 1101 8 0.30237719705697685 1101 9 0.3575591309868784 1101 10-11 0.31400073950421614 1101 12-13 0.2762278782299106 1101 14-15 0.3907251473837974 1101 16-17 0.3595502138523088 1101 18-19 0.3850402264912418 1101 20-21 0.4001384770653189 1101 22-23 0.48308960052915495 1101 24-25 0.36407787530202285 1101 26-27 0.43926420369282937 1101 28-29 0.31770647444957234 1101 30-31 0.17277305487122874 1101 32-33 0.3223320364447986 1101 34-35 0.149944204243198 1101 36-37 0.07151858378598064 1101 38-39 -0.011754240059225651 1101 40-41 0.10646636739711113 1101 42-43 0.32475968634664554 1101 44-45 0.2579548664037432 1101 46-47 0.041845105870315535 1101 48-49 -0.0934725540853023 1101 50-51 -0.10467200747032024 1101 52-53 -0.28422583257051315 1101 54-55 -0.08131596079629588 1101 56-57 -3.7036297268786598 1101 58-59 -10.474291760246253 1101 60-61 -11.935807894644185 1101 62-63 -4.226669361604699 1101 64-65 -1.3306144445446861 1101 66-67 -2.1381189729091403 1101 68-69 -4.850678834292534 1101 70-71 -4.853099019852937 1101 72-73 -3.803031763061206 1101 74-75 -6.30333713154689 1101 76-77 -4.940112298926 1101 78-79 1.275472961836165 1101 80-81 1.7684855677595976 1101 82-83 0.3467673715713069 1101 84-85 -0.02252842458074511 1101 86-87 -0.10255401721633461 1101 88-89 -0.25731879758888354 1101 90-91 1.21354738288046 1101 92-93 0.74951219208587 1101 94-95 -8.805177000332652 1101 96-97 -9.844371816604285 1101 98-99 -8.917022769773563 1101 100-101 -9.658551372096486 1102 1 0.16599615008533775 1102 2 0.2086637059350558 1102 3 0.25441236430093994 1102 4 0.16078607615203566 1102 5 0.20958787663067113 1102 6 0.21303342501354905 1102 7 0.30175390465941376 1102 8 0.35106718276449556 1102 9 0.27071501827882827 1102 10-11 0.22578663260458853 1102 12-13 0.31247292856124176 1102 14-15 0.4410914721531185 1102 16-17 0.35048362977309466 1102 18-19 0.3738508934456206 1102 20-21 0.28715652823097315 1102 22-23 0.1883833438793161 1102 24-25 0.2080855616551318 1102 26-27 0.058499086797553446 1102 28-29 0.17396654737871842 1102 30-31 0.009152071504196613 1102 32-33 0.03491744849272749 1102 34-35 0.017912056663831777 1102 36-37 -0.12199604764798977 1102 38-39 -0.024760627154783776 1102 40-41 -0.21493154014176952 1102 42-43 -0.1610745715459032 1102 44-45 -0.28147799044850075 1102 46-47 -0.26746642411432475 1102 48-49 -0.1426145826299745 1102 50-51 -0.16024781991792736 1102 52-53 -0.11455253399237364 1102 54-55 -0.3792690614331846 1102 56-57 -3.78368875567876 1102 58-59 -10.265531651028024 1102 60-61 -8.197029887866034 1102 62-63 -2.6786285713735865 1102 64-65 -1.10066540958519 1102 66-67 -2.3375869640949 1102 68-69 -5.295815262835642 1102 70-71 -5.051345368791877 1102 72-73 -3.8603031965320476 1102 74-75 -4.672247254424981 1102 76-77 -4.619641550419754 1102 78-79 1.0984530734678906 1102 80-81 1.5360102167141605 1102 82-83 0.2014225461889687 1102 84-85 -0.18337183057240836 1102 86-87 -0.3506674722773404 1102 88-89 -0.344759026216618 1102 90-91 1.0213382918906184 1102 92-93 0.49935652816136056 1102 94-95 -9.132378019980102 1102 96-97 -9.945239927338207 1102 98-99 -9.046906278561913 1102 100-101 -9.852631788274875 1103 1 0.17283680241751043 1103 2 0.19338402981967917 1103 3 0.2207153797713275 1103 4 0.20591505134009935 1103 5 0.29334065327348213 1103 6 0.2661342428139335 1103 7 0.25712695478728165 1103 8 0.3218020416353369 1103 9 0.36070676353494235 1103 10-11 0.3785238164924962 1103 12-13 0.35375774418800887 1103 14-15 0.37204287286700577 1103 16-17 0.38268335309398793 1103 18-19 0.3726415630961597 1103 20-21 0.34920435804221484 1103 22-23 0.42772580582556685 1103 24-25 0.4528112366048944 1103 26-27 0.40578710269312523 1103 28-29 0.45064134780265164 1103 30-31 0.4257381438016452 1103 32-33 0.37744410530071804 1103 34-35 0.3766072263942206 1103 36-37 0.3110703719294392 1103 38-39 0.37043679188961676 1103 40-41 0.3186871487643188 1103 42-43 0.27897037325309526 1103 44-45 0.2890245741220596 1103 46-47 0.2903751159139176 1103 48-49 0.21478990189445568 1103 50-51 0.22443517095414478 1103 52-53 0.2617905211339675 1103 54-55 0.23082822217534016 1103 56-57 0.7469856029712005 1103 58-59 -8.259672908777219 1103 60-61 -7.386239091698059 1103 62-63 -2.103840073259942 1103 64-65 -0.30219824085864033 1103 66-67 -1.7510556261419516 1103 68-69 -3.6031843328495583 1103 70-71 -4.021056629050676 1103 72-73 -3.0655746179035397 1103 74-75 -3.9781322334873828 1103 76-77 -0.6683205321410988 1103 78-79 3.2842203290973977 1103 80-81 2.6871486661911845 1103 82-83 1.0671057817996825 1103 84-85 0.7492951257707823 1103 86-87 0.7069478919165668 1103 88-89 0.3840904221881196 1103 90-91 1.8253639832254187 1103 92-93 1.3322483122023279 1103 94-95 -7.3429393736751365 1103 96-97 -8.676966817684926 1103 98-99 -8.086517538198498 1103 100-101 -9.548376498912916 1104 1 0.17251265653593606 1104 2 0.23475334706252582 1104 3 0.24215539984128753 1104 4 0.16073731864895535 1104 5 0.24818651473894704 1104 6 0.1705259416529401 1104 7 0.22556404599611568 1104 8 0.2281698755324726 1104 9 0.3188798988499215 1104 10-11 0.2665880105180989 1104 12-13 0.2985043110888981 1104 14-15 0.43332630538871086 1104 16-17 0.33659211837295544 1104 18-19 0.3297154795071435 1104 20-21 0.3948544218559462 1104 22-23 0.3054558090311872 1104 24-25 0.33163931385036705 1104 26-27 0.33579135362344914 1104 28-29 0.4229240376559673 1104 30-31 0.3567913889439396 1104 32-33 0.4844524657955347 1104 34-35 0.36821746287338186 1104 36-37 0.29112469822169373 1104 38-39 0.25565420656737103 1104 40-41 0.21736398447929872 1104 42-43 0.35708266152789037 1104 44-45 0.3709831081384607 1104 46-47 0.3090301792995689 1104 48-49 0.35756441542675077 1104 50-51 0.44569012124713225 1104 52-53 0.2851774573381576 1104 54-55 0.31181556296456137 1104 56-57 0.7535721556956716 1104 58-59 -8.036511958798819 1104 60-61 -7.285268346461194 1104 62-63 -1.9416455777667245 1104 64-65 -0.14060803452425574 1104 66-67 -1.387635777582588 1104 68-69 -3.2096573449174244 1104 70-71 -3.430799771314481 1104 72-73 -2.8176707148128024 1104 74-75 -3.5588118547065903 1104 76-77 -0.12307584978614372 1104 78-79 3.7729735322281535 1104 80-81 3.1931406859903646 1104 82-83 1.577746696655641 1104 84-85 1.2151041564591125 1104 86-87 1.0832872834063458 1104 88-89 0.966539206939725 1104 90-91 2.5450889470377582 1104 92-93 2.1573559294314357 1104 94-95 -6.201644771261565 1104 96-97 -7.8074508164663925 1104 98-99 -5.630959073471264 1104 100-101 -4.932014449068518 1105 1 0.22012553035887805 1105 2 0.16200291006120793 1105 3 0.11853094542178866 1105 4 0.19298670086705982 1105 5 0.2798059727183997 1105 6 0.2216803413643973 1105 7 0.17746646350033046 1105 8 0.13534284470903657 1105 9 0.22706573182029643 1105 10-11 0.16205900893027803 1105 12-13 0.19354237204928637 1105 14-15 0.2605994338984843 1105 16-17 0.2515119045060814 1105 18-19 0.32952844304059425 1105 20-21 0.3801521339620493 1105 22-23 0.43208634388454925 1105 24-25 0.32996189673283993 1105 26-27 0.2741908416449945 1105 28-29 0.34886345872663327 1105 30-31 0.37405470210307357 1105 32-33 0.33764351874335574 1105 34-35 0.3864536415090143 1105 36-37 0.3346239519969245 1105 38-39 0.4899528720323829 1105 40-41 0.4368056887543972 1105 42-43 0.3378387912946508 1105 44-45 0.3163906264625709 1105 46-47 0.32898135967848674 1105 48-49 0.3324125178760369 1105 50-51 0.37808178319397 1105 52-53 0.44763500715354354 1105 54-55 0.38301852812060844 1105 56-57 0.9209631925456492 1105 58-59 -8.274349539424737 1105 60-61 -7.544266416197573 1105 62-63 -1.9755575501399072 1105 64-65 -0.1722907410744341 1105 66-67 -1.8391204786695496 1105 68-69 -3.398005234124451 1105 70-71 -3.953048244211237 1105 72-73 -2.9647959539776174 1105 74-75 -3.7996165026292843 1105 76-77 -0.2814821907213556 1105 78-79 3.796921293243525 1105 80-81 3.2621648758101642 1105 82-83 1.7936455450489142 1105 84-85 1.4319836039936824 1105 86-87 1.2663047564643755 1105 88-89 1.130116857416894 1105 90-91 2.515189712256795 1105 92-93 2.12694154390854 1105 94-95 -5.92703854041039 1105 96-97 -7.618113806309086 1105 98-99 -5.315173836062041 1105 100-101 -4.952594864059927 1106 1 0.026707539688270998 1106 2 0.1662200540182397 1106 3 0.06531371323621471 1106 4 0.10978548501568497 1106 5 0.1409914338166729 1106 6 0.18883729232625512 1106 7 0.15812608650508508 1106 8 0.20304107425967288 1106 9 0.11117328811906901 1106 10-11 0.1217386161500329 1106 12-13 0.10813619378817663 1106 14-15 0.1086918196953448 1106 16-17 0.14405349835416104 1106 18-19 0.12819436942874773 1106 20-21 0.09828603323990848 1106 22-23 0.07510861302846195 1106 24-25 0.08262600306936463 1106 26-27 0.05062552934149522 1106 28-29 0.11866968942413081 1106 30-31 0.0975136594580519 1106 32-33 0.1528192983366452 1106 34-35 0.1080416731122611 1106 36-37 0.13114575461116118 1106 38-39 0.24321675217936445 1106 40-41 0.2847542149622271 1106 42-43 0.253929944495745 1106 44-45 0.3757673824583776 1106 46-47 0.15320246741033827 1106 48-49 0.22692763695302176 1106 50-51 0.20481229687935354 1106 52-53 0.1750584904263306 1106 54-55 0.1233357462757354 1106 56-57 0.7415809004843439 1106 58-59 -8.506266419126778 1106 60-61 -7.921303221118659 1106 62-63 -2.0538811598068314 1106 64-65 -0.30173242466325334 1106 66-67 0.531931456895375 1106 68-69 -2.517968658837912 1106 70-71 -2.465813692192402 1106 72-73 -1.0466687344314636 1106 74-75 -0.9004535120683386 1106 76-77 2.3002649066247685 1106 78-79 4.551166252724993 1106 80-81 3.344086827902167 1106 82-83 1.7380880032662915 1106 84-85 1.3709087240010227 1106 86-87 1.2191083460139858 1106 88-89 1.116225819563848 1106 90-91 2.528329956278313 1106 92-93 2.0353318777859677 1106 94-95 -6.356188418868314 1106 96-97 -7.845413108662685 1106 98-99 -5.882635871501471 1106 100-101 -5.217080643391835 1107 1 -0.010141784786021901 1107 2 0.07097100930308287 1107 3 -0.014934205735830375 1107 4 0.025734290849747765 1107 5 0.0015921015979003528 1107 6 0.11923213674216981 1107 7 0.06449180451362224 1107 8 0.2088895492370355 1107 9 0.07676916151208957 1107 10-11 -0.022321172785105148 1107 12-13 0.03754611278257158 1107 14-15 0.03290550609684573 1107 16-17 0.0868470857701098 1107 18-19 0.12162693581965556 1107 20-21 0.12378446398594889 1107 22-23 0.12170025610242874 1107 24-25 0.02382214304793706 1107 26-27 0.09561962517815914 1107 28-29 0.007669722158503589 1107 30-31 0.08539410673734693 1107 32-33 0.16715840130301984 1107 34-35 0.1092779446351102 1107 36-37 0.1420421095986768 1107 38-39 0.16607101395536006 1107 40-41 0.051430083181621455 1107 42-43 -0.002315434767901081 1107 44-45 0.06350425757114664 1107 46-47 0.1945826584034407 1107 48-49 0.08993293393363189 1107 50-51 0.12587809416894657 1107 52-53 0.09624803961811068 1107 54-55 0.2371200315406341 1107 56-57 0.7873042343446244 1107 58-59 -8.411136036517831 1107 60-61 -7.513090306370145 1107 62-63 -2.0771795012918233 1107 64-65 -0.2995510478874124 1107 66-67 0.84884815230442 1107 68-69 -2.409912439945547 1107 70-71 -2.317679072478004 1107 72-73 -0.723176920819391 1107 74-75 -0.6569720936160479 1107 76-77 2.595996074778853 1107 78-79 4.509900881541142 1107 80-81 3.278763587933657 1107 82-83 1.5581801824654313 1107 84-85 1.2901956874966096 1107 86-87 1.0570167073312184 1107 88-89 0.9687917952956973 1107 90-91 2.356694401239057 1107 92-93 2.008781655671264 1107 94-95 -6.731066114064985 1107 96-97 -8.040140768649943 1107 98-99 -6.203886228785189 1107 100-101 -5.535168740375003 1108 1 -0.09698943429162554 1108 2 -0.06771534773588073 1108 3 -0.08127714776062689 1108 4 -0.009873133383074162 1108 5 0.027744472186618907 1108 6 -0.0788206657198458 1108 7 -0.09303722791397462 1108 8 -0.14553657245107132 1108 9 -0.1666160396397771 1108 10-11 -0.11395919216530359 1108 12-13 -0.04259428468306936 1108 14-15 -0.15383926628860678 1108 16-17 -0.18711780558917468 1108 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4.446676099713745 2315 98-99 6.567321724712553 2315 100-101 6.691876265944565 2316 1 -0.49471555109245813 2316 2 -0.40995812679419785 2316 3 -0.46198311013002424 2316 4 -0.3444088457336818 2316 5 -0.5808007724162181 2316 6 -0.5614664772587759 2316 7 -0.4534511961605787 2316 8 -0.3531465592029406 2316 9 -0.5172727891906135 2316 10-11 -0.6148329024098018 2316 12-13 -0.5832338594852118 2316 14-15 -0.679092220213434 2316 16-17 -0.7771375201007444 2316 18-19 -0.6744474809860037 2316 20-21 -0.6828733210603701 2316 22-23 -0.8217151495690729 2316 24-25 -0.7942017018381975 2316 26-27 -0.7454337363969756 2316 28-29 -0.7542600781970847 2316 30-31 -0.8264284204865433 2316 32-33 -0.6627991614575421 2316 34-35 -0.6537218638842148 2316 36-37 -0.5577091184001475 2316 38-39 -0.7502175325190308 2316 40-41 -0.8673565981715967 2316 42-43 -0.8610803767580535 2316 44-45 -0.6697305294610558 2316 46-47 -0.8822226393815029 2316 48-49 -0.8084390312314085 2316 50-51 -0.6967138643305759 2316 52-53 -0.6727576119283398 2316 54-55 -0.5280264339783187 2316 56-57 0.18730182650377714 2316 58-59 3.218840836744132 2316 60-61 3.2533157847792396 2316 62-63 0.5763043482112451 2316 64-65 0.07269813726866658 2316 66-67 -1.8865082100519324 2316 68-69 -2.958934789677169 2316 70-71 -3.0478774479880464 2316 72-73 -0.5499271822421115 2316 74-75 5.70944113506156 2316 76-77 5.8042702472077785 2316 78-79 4.900939299215153 2316 80-81 3.1953848368615922 2316 82-83 1.3201334879340614 2316 84-85 0.9791636582447829 2316 86-87 0.7755964863602252 2316 88-89 0.7852562288110079 2316 90-91 2.488556031033905 2316 92-93 4.041765683278115 2316 94-95 6.829074727425706 2316 96-97 4.109887447040617 2316 98-99 6.496362810753851 2316 100-101 6.5728127933966825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 15.0 4 41.0 5 102.0 6 201.0 7 665.0 8 1500.0 9 2605.0 10 4434.0 11 6570.0 12 9367.0 13 12430.0 14 15693.0 15 20524.0 16 27490.0 17 35876.0 18 40000.0 19 40399.0 20 42887.0 21 44569.0 22 44897.0 23 45558.0 24 47709.0 25 51768.0 26 64617.0 27 90890.0 28 128094.0 29 170649.0 30 208110.0 31 233121.0 32 244188.0 33 244409.0 34 250741.0 35 267292.0 36 270079.0 37 207754.0 38 82797.0 39 5573.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.44193555028508 20.106303519228362 14.478825531070388 23.97293539941617 2 15.430650790967142 21.57423333876652 39.29870065883276 23.69641521143358 3 18.461937143572488 25.354947056141985 31.014158065954 25.16895773433153 4 12.913771979461597 17.86454331680287 37.84319104446287 31.378493659272667 5 13.181072144494951 37.76140734960794 35.318020777868455 13.739499728028647 6 30.939570842270886 37.599198817391844 17.719382957121006 13.741847383216262 7 26.69266865950043 33.218136339387755 22.360060453114926 17.729134547996892 8 24.933366850957363 35.722420084930064 20.677820837052046 18.666392227060534 9 25.50256966229465 16.530026295552837 20.94500001518414 37.02240402696838 10-11 24.21681059542404 26.601546959983725 28.815613612950923 20.366028831641316 12-13 25.117061268671847 24.206029857414197 28.720037899608553 21.9568709743054 14-15 22.251119119760197 25.778567057683027 26.6460757291424 25.32423809341438 16-17 21.601110264360948 28.621480801256975 27.494395786772152 22.283013147609925 18-19 21.569589115976797 27.771029251487207 29.178856192279014 21.480525440256983 20-21 22.554181222350106 26.98430533749446 28.84608311662194 21.61543032353349 22-23 22.040198149627198 26.898177532267137 28.88272750309672 22.178896815008947 24-25 22.199775915600387 26.96441221879154 28.914935152687654 21.920876712920418 26-27 22.06019035201778 26.97069743720042 29.106518408517708 21.862593802264094 28-29 21.911415540367834 27.432899111665172 28.872678756442593 21.7830065915244 30-31 22.095509369694717 27.05098842998856 28.738269111170577 22.115233089146148 32-33 22.04474670848331 27.08764740391444 28.713407696900887 22.154198190701365 34-35 22.3653783793542 27.099030317386564 28.584783706821586 21.950807596437645 36-37 22.349616020244316 26.859754980406304 28.843238084334548 21.947390915014832 38-39 22.09139621997972 27.234651846236062 28.709969092209224 21.96398284157499 40-41 22.336100245080907 27.109048338383886 28.68677566130812 21.868075755227093 42-43 22.08317396792428 27.173680855465072 28.373763598829772 22.36938157778087 44-45 22.247530039241752 27.144541971854963 28.396687873221342 22.21124011568194 46-47 22.476339748496425 27.157141016568044 28.126532932864777 22.239986302070747 48-49 22.22661594338831 27.247052093567802 28.23406909777525 22.29226286526864 50-51 22.016032945861753 27.444107467774813 28.32021116579314 22.219648420570294 52-53 22.42672157947941 27.465910771406172 27.79660650701975 22.310761142094666 54-55 22.399775835468635 27.25398449078528 27.837014830644375 22.50922484310171 56-57 22.084917092988825 27.484703220013973 27.918341312769474 22.512038374227732 58-59 21.196455600875158 27.054963742430903 29.467201666033432 22.281378990660507 60-61 21.425694771848715 27.25811325418073 29.07482536009297 22.241366613877577 62-63 22.108153224463504 27.291586982119338 28.259122590672508 22.34113720274465 64-65 22.019087262661937 27.506413767016348 27.89684118389214 22.577657786429572 66-67 21.693555952399226 27.31042328665019 28.375181010177503 22.62083975077308 68-69 21.018144691084174 26.872299588665054 29.827778324842313 22.281777395408458 70-71 21.893670998015796 26.776128343162238 28.72946708377319 22.600733575048775 72-73 21.231612626388767 26.94908651036619 29.1348035788579 22.68449728438714 74-75 21.330304389075568 27.2978873489743 28.668431082518335 22.703377179431797 76-77 20.367033537044456 27.499727218124548 29.012859328511297 23.120379916319695 78-79 21.71065054654882 27.474552383738025 27.968498347603653 22.846298722109502 80-81 21.852758050287278 27.361213102973025 27.734189676790205 23.05183916994949 82-83 22.1396353268182 27.308242896127616 27.66510466533665 22.88701711171754 84-85 21.998930010505873 27.47593662463361 27.40345673080249 23.121676634058026 86-87 21.796666663292367 27.327955372848418 27.62339227224398 23.251985691615236 88-89 21.92354075322798 27.280371491872437 27.381126717410947 23.414961037488638 90-91 21.616994208166457 27.33387600564068 27.65360580019537 23.395523985997496 92-93 21.596510169164226 27.365676015464746 27.650255292543243 23.387558522827785 94-95 20.41545274661865 27.491222817302774 28.370116260414335 23.723208175664237 96-97 19.995938274116412 27.562069106286515 28.409937231926953 24.032055387670123 98-99 20.79165811387681 27.393596927345516 27.90886962084697 23.905875337930702 100-101 20.02935223187245 27.338499099921442 28.37139226288741 24.26075640531869 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 594.0 1 456.5 2 346.5 3 397.5 4 437.5 5 514.0 6 636.5 7 931.5 8 1363.0 9 1703.5 10 1815.0 11 1917.5 12 2111.5 13 2265.5 14 2469.5 15 2709.0 16 3002.5 17 3387.0 18 3741.0 19 4095.0 20 4761.5 21 5617.5 22 6773.5 23 8451.0 24 10668.0 25 13615.0 26 17745.5 27 22913.5 28 28186.5 29 33405.0 30 39066.0 31 45592.5 32 52118.5 33 58029.0 34 64653.5 35 71674.0 36 77776.5 37 82643.5 38 87128.0 39 90828.0 40 93634.0 41 96993.0 42 100324.0 43 103062.0 44 105705.0 45 108086.0 46 111227.5 47 115041.5 48 117237.5 49 115824.5 50 112015.0 51 108176.0 52 104662.0 53 100558.0 54 95060.0 55 87445.0 56 78470.0 57 70119.0 58 61877.0 59 53036.5 60 43746.0 61 34713.0 62 26545.0 63 19602.0 64 14024.0 65 9813.5 66 6871.5 67 4887.0 68 3282.5 69 2136.5 70 1396.0 71 810.5 72 432.5 73 242.5 74 139.5 75 87.0 76 45.5 77 18.5 78 9.5 79 6.0 80 3.5 81 3.0 82 2.5 83 0.5 84 0.5 85 0.5 86 1.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026386657664159936 2 0.011742400085840994 3 2.6994023185841363E-4 4 0.0 5 0.06444823035619626 6 0.0 7 0.0 8 1.3497011592920682E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 5.904942571902798E-4 16-17 1.6871264491150852E-5 18-19 3.0368276084071535E-4 20-21 0.0 22-23 0.0 24-25 1.1809885143805597E-4 26-27 1.5184138042035768E-4 28-29 0.001366572423783219 30-31 0.006141140274778911 32-33 0.006934089705863001 34-35 0.002429462086725723 36-37 0.08741002132865257 38-39 0.08069525806117453 40-41 0.08624590407876316 42-43 0.08963702824148448 44-45 0.09252201446947127 46-47 0.08730879374170566 48-49 0.07836702356139572 50-51 0.07269827869236901 52-53 0.06185005562455903 54-55 0.050917476234293275 56-57 0.06910469935575389 58-59 0.7106514028962562 60-61 0.3388256047757826 62-63 0.0037285494525443386 64-65 0.002429462086725723 66-67 1.0849572768969291 68-69 2.3411916309080216 70-71 2.3199675801781536 72-73 1.7835625969465037 74-75 1.2696301380170663 76-77 0.7324828191478054 78-79 0.5579495879868499 80-81 0.03713365314502303 82-83 2.1932643838496108E-4 84-85 0.0012990873658186156 86-87 0.0014171862172566716 88-89 3.3742528982301704E-5 90-91 0.32792676791449915 92-93 0.0515248417559747 94-95 0.9408091930845361 96-97 1.6399543935978276 98-99 0.6300911149510109 100-101 0.5619480776712527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2963619.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.59699967124572 #Duplication Level Percentage of deduplicated Percentage of total 1 66.70442443465177 29.081127701481485 2 15.155610439431186 13.214782866908193 3 6.421981933899636 8.399374327829051 4 3.4756303817392893 6.061082264402377 5 2.1159908198997512 4.612542553976421 6 1.3975090650873048 3.6556321350704484 7 0.9671411838011732 2.951511771055957 8 0.6925490937587914 2.415445009033884 9 0.5028131867877041 1.9729031705173392 >10 2.4014212832856163 17.78605439986561 >50 0.10607046070397397 3.1620960591741936 >100 0.05451494338164424 4.257643304198115 >500 0.0030244315949428096 0.90946929572626 >1k 0.0010856933929249111 0.818387129008539 >5k 2.326485841981952E-4 0.7019480117522634 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8731 0.29460602054447615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7013 0.23663635575288186 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5018 0.16932001043318995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4583 0.15464201032588873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.748505796460341E-5 2 0.0 0.0 0.0 0.0 6.748505796460341E-5 3 0.0 0.0 0.0 0.0 6.748505796460341E-5 4 0.0 0.0 0.0 0.0 1.6871264491150853E-4 5 3.3742528982301704E-5 0.0 0.0 0.0 1.6871264491150853E-4 6 3.3742528982301704E-5 0.0 0.0 0.0 2.0245517389381023E-4 7 3.3742528982301704E-5 0.0 0.0 0.0 2.0245517389381023E-4 8 3.3742528982301704E-5 0.0 0.0 0.0 2.0245517389381023E-4 9 3.3742528982301704E-5 0.0 0.0 0.0 2.6994023185841363E-4 10-11 8.435632245575427E-5 0.0 0.0 5.0613793473452556E-5 2.868114963495645E-4 12-13 3.0368276084071535E-4 0.0 0.0 1.0122758694690511E-4 3.0368276084071535E-4 14-15 3.0368276084071535E-4 0.0 0.0 1.1809885143805596E-4 4.2178161227877134E-4 16-17 3.880390832964696E-4 0.0 0.0 1.8558390940265937E-4 5.061379347345256E-4 18-19 4.3865287676992217E-4 0.0 0.0 2.1932643838496108E-4 5.398804637168273E-4 20-21 4.723954057522239E-4 0.0 0.0 4.2178161227877134E-4 5.398804637168273E-4 22-23 5.061379347345256E-4 0.0 0.0 8.435632245575427E-4 6.411080506637324E-4 24-25 6.748505796460341E-4 0.0 0.0 0.0011978597788717105 7.085931086283358E-4 26-27 7.085931086283358E-4 0.0 0.0 0.0019908092099558006 7.085931086283358E-4 28-29 7.085931086283358E-4 0.0 0.0 0.0048420529089602945 7.085931086283358E-4 30-31 7.254643731194867E-4 0.0 0.0 0.013142715038606517 7.423356376106375E-4 32-33 7.423356376106376E-4 0.0 0.0 0.031093740457191023 8.435632245575427E-4 34-35 7.760781665929392E-4 0.0 0.0 0.05916752457046605 8.773057535398443E-4 36-37 8.435632245575427E-4 0.0 0.0 0.09893309497610861 9.616620759955985E-4 38-39 8.941770180309952E-4 1.6871264491150852E-5 0.0 0.1561604241300923 0.0010966321919248056 40-41 9.785333404867494E-4 6.748505796460341E-5 0.0 0.21838164757345663 0.001147245985398258 42-43 9.785333404867494E-4 1.0122758694690511E-4 0.0 0.28583296300907773 0.0011809885143805599 44-45 0.0010966321919248054 1.0122758694690511E-4 0.0 0.3687214854541019 0.0012147310433628614 46-47 0.0011135034564159563 1.0122758694690511E-4 0.0 0.4655456723688166 0.001265344836836314 48-49 0.0011809885143805596 1.1809885143805596E-4 0.0 0.559991010990279 0.0013497011592920683 50-51 0.0011809885143805596 1.3497011592920682E-4 0.0 0.6552967841007903 0.0013497011592920683 52-53 0.0011978597788717105 1.3497011592920682E-4 0.0 0.7509399825011245 0.0014678000107301243 54-55 0.0012147310433628614 1.3497011592920682E-4 0.0 0.8623071994072112 0.0016533839201327836 56-57 0.0012147310433628614 1.3497011592920682E-4 0.0 0.9725102990634086 0.0017039977136062362 58-59 0.0012147310433628614 1.3497011592920682E-4 0.0 1.0520414398746938 0.0017208689780973871 60-61 0.0012147310433628614 1.6871264491150853E-4 0.0 1.1143976334339873 0.0017208689780973871 62-63 0.001265344836836314 1.6871264491150853E-4 0.0 1.1706464292474843 0.0017208689780973871 64-65 0.0012822161013274647 1.6871264491150853E-4 0.0 1.239076278023592 0.0017208689780973871 66-67 0.0012822161013274647 2.1932643838496108E-4 0.0 1.300116512952576 0.001737740242588538 68-69 0.0012822161013274647 2.3619770287611194E-4 0.0 1.3709083387574448 0.0017546115070796887 70-71 0.0012822161013274647 2.3619770287611194E-4 0.0 1.480419716569505 0.0017883540360619904 72-73 0.0012822161013274647 2.3619770287611194E-4 0.0 1.6289543291495971 0.0018558390940265938 74-75 0.0013497011592920683 2.3619770287611194E-4 0.0 1.82865948693135 0.0018727103585177447 76-77 0.0014171862172566716 2.3619770287611194E-4 0.0 2.0889156129718427 0.0019064528875000464 78-79 0.0014171862172566716 2.3619770287611194E-4 0.0 2.415155254437227 0.0019233241519911973 80-81 0.0014846712752212751 2.3619770287611194E-4 0.0 2.798166700915334 0.002058294267920404 82-83 0.001501542539712426 2.3619770287611194E-4 0.0 3.1839956485634624 0.002328234499778818 84-85 0.0015184138042035767 2.868114963495645E-4 0.0 3.5340575154903515 0.002328234499778818 86-87 0.0015858988621681802 3.0368276084071535E-4 0.0 3.8597404052275275 0.002328234499778818 88-89 0.0015858988621681802 3.0368276084071535E-4 0.0 4.179029085722558 0.0023619770287611193 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14375 0.0 23.926842 1 GGTATCA 10775 0.0 21.762302 1 GTACATG 7320 0.0 20.818869 1 ATCAACG 16370 0.0 20.572664 3 TCAACGC 16400 0.0 20.535032 4 TACATGG 7025 0.0 20.345419 2 CAACGCA 16535 0.0 20.338848 5 ACATGGG 6810 0.0 20.293715 3 GAGTACT 7810 0.0 20.279198 12-13 AACGCAG 16770 0.0 20.040981 6 TATCAAC 17010 0.0 19.883152 2 AGAGTAC 11505 0.0 19.768476 10-11 AGTACTT 8235 0.0 19.633287 12-13 CGCAGAG 17770 0.0 18.196974 8 ACGCAGA 18070 0.0 18.181812 7 GTACTTT 8715 0.0 18.011065 14-15 CATGGGG 4370 0.0 17.053623 4 GCAGAGT 18765 0.0 16.754822 9 TCTATAC 1030 0.0 16.48566 3 TACTTTT 10335 0.0 15.507114 14-15 >>END_MODULE