FastQCFastQC Report
Fri 27 May 2016
SRR939673_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939673_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113343
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139270.659003294779882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93930.44446168937082153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68060.32204900009132453No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52880.25021967565132586No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41130.19462056088386975No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31810.15051981623427907No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28780.1361823423836074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21830.10329605747860145No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21650.10244432635875957No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.10003108818587422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112000.028.5575641
TACATGG111100.028.4899062
ACATGGG111900.027.2260253
CATGGGA84000.024.9702154
GAGTACT98700.023.33967412-13
AGTACTT104450.021.80497212-13
GTATCAA243350.021.2751521
ATGGGAT32650.021.2201755
GTACTTT113500.020.29746414-15
ATGGGAG32950.020.162855
CATGGGG28550.019.9459344
CATTCCG4550.019.8134359
TCAACGC276250.018.8444564
CAACGCA276150.018.8340955
TATCAAC277300.018.8244422
ATCAACG276400.018.7655543
AACGCAG280400.018.6292786
AGTACAT77850.018.2869762
GTATAGC9500.017.9828361
ACTTTTT135350.017.9515216-17
Produced by FastQC (version 0.11.5)