##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939673_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2113343 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.274573507471338 31.0 30.0 33.0 26.0 34.0 2 30.41213565426909 31.0 30.0 34.0 26.0 34.0 3 30.43787922736631 31.0 30.0 34.0 26.0 34.0 4 34.04667344581547 35.0 35.0 37.0 28.0 37.0 5 34.07021245486416 35.0 35.0 37.0 30.0 37.0 6 34.19601124852899 36.0 35.0 37.0 30.0 37.0 7 34.017793136277454 36.0 35.0 37.0 29.0 37.0 8 34.03040869371418 36.0 35.0 37.0 29.0 37.0 9 35.28589301405404 38.0 35.0 39.0 28.0 39.0 10-11 35.19245976635123 37.0 34.5 39.0 28.5 39.0 12-13 35.05787938824885 37.0 34.5 39.0 27.0 39.0 14-15 35.691220260979875 38.0 34.0 40.0 27.0 41.0 16-17 35.45885357937637 38.0 34.0 40.0 27.0 41.0 18-19 35.52753339140878 38.0 34.0 40.0 27.0 41.0 20-21 35.66764363380672 38.0 34.0 40.0 27.0 41.0 22-23 35.51566475484576 38.0 34.0 40.0 27.0 41.0 24-25 35.39095594042236 38.0 34.0 40.0 27.0 41.0 26-27 35.48628145076308 38.0 34.0 40.0 27.0 41.0 28-29 35.36051909226283 38.0 34.0 40.0 26.5 41.0 30-31 35.1018244553771 38.0 34.0 40.0 25.0 41.0 32-33 34.82856474315811 38.0 34.0 40.0 25.0 41.0 34-35 34.57211773006085 38.0 33.0 40.0 24.0 41.0 36-37 34.241600156718526 38.0 33.0 40.0 24.0 41.0 38-39 34.05434494069349 38.0 33.0 40.0 23.5 41.0 40-41 33.76757558995392 38.0 33.0 40.0 22.0 41.0 42-43 33.41350457545226 37.5 32.0 40.0 18.5 41.0 44-45 33.08869241765298 37.0 31.5 40.0 17.0 41.0 46-47 32.54492100903639 37.0 31.0 40.0 12.0 41.0 48-49 32.12191821204603 37.0 30.5 40.0 9.0 41.0 50-51 31.49693282160066 36.0 30.0 38.5 8.0 40.5 52-53 31.54845521999978 36.5 30.0 39.0 8.0 40.0 54-55 31.59959221006718 37.0 30.0 39.0 7.0 40.0 56-57 31.4919755098912 36.0 30.0 39.0 2.0 40.5 58-59 31.125318038766068 36.0 29.5 39.0 2.0 40.0 60-61 30.718175185003098 35.5 28.5 39.0 2.0 40.0 62-63 30.329433508900355 35.0 28.0 39.0 2.0 40.0 64-65 29.737899148410833 34.5 27.5 38.0 2.0 40.0 66-67 29.220811529410987 34.0 26.5 38.0 2.0 40.0 68-69 28.715782057148317 34.0 26.0 37.5 2.0 39.5 70-71 28.17537806215082 34.0 26.0 37.0 2.0 39.0 72-73 27.438882377351902 33.0 24.5 36.0 2.0 39.0 74-75 26.99362266324018 32.0 23.5 36.0 2.0 38.0 76-77 26.625636964752054 32.0 22.5 35.0 2.0 38.0 78-79 26.052311432644863 32.0 21.5 35.0 2.0 37.0 80-81 25.383296984919156 31.0 19.5 35.0 2.0 36.5 82-83 24.72645945310345 31.0 17.5 34.0 2.0 36.0 84-85 24.0662996967364 30.5 10.0 34.0 2.0 35.5 86-87 23.397411825718777 30.0 4.5 34.0 2.0 35.0 88-89 22.591182784810606 29.5 2.0 34.0 2.0 35.0 90-91 21.344480048908295 28.5 2.0 33.5 2.0 35.0 92-93 19.719671392670286 26.0 2.0 33.0 2.0 34.0 94-95 19.503639494393482 26.0 2.0 32.0 2.0 34.0 96-97 18.889722586442428 25.5 2.0 32.0 2.0 34.0 98-99 17.92354364625146 24.5 2.0 32.0 2.0 34.0 100-101 16.27812191395339 18.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9510292843900601 1101 2 1.0322309699967072 1101 3 1.0158762192582138 1101 4 1.0857105408414043 1101 5 0.8606990078060264 1101 6 0.8418692806308599 1101 7 0.8024717350685435 1101 8 0.8257215750336258 1101 9 1.084301845985017 1101 10-11 0.9511533313704916 1101 12-13 0.9769745698736259 1101 14-15 1.2190067496901094 1101 16-17 0.9941603077962213 1101 18-19 0.9623907611325393 1101 20-21 1.286122175233217 1101 22-23 1.414678355131052 1101 24-25 1.2927678662386697 1101 26-27 1.2575243204022755 1101 28-29 1.4256461166900394 1101 30-31 1.4596642215458147 1101 32-33 1.449082337536602 1101 34-35 1.2783299715236254 1101 36-37 1.1910560596270727 1101 38-39 1.1310459368158519 1101 40-41 1.3712161930353588 1101 42-43 1.3985631918306112 1101 44-45 1.3922514700400015 1101 46-47 1.1127779909229432 1101 48-49 1.0399409931640164 1101 50-51 1.1490226671517227 1101 52-53 1.064724804133789 1101 54-55 1.2321966843412504 1101 56-57 1.139848351933022 1101 58-59 1.0265288266217247 1101 60-61 0.8772198279135957 1101 62-63 0.6694330331189242 1101 64-65 0.7799792482612915 1101 66-67 0.6041396683832154 1101 68-69 0.7608142912491545 1101 70-71 0.5477129787282031 1101 72-73 0.7443975070673332 1101 74-75 0.5090863298922237 1101 76-77 -0.08322458724696702 1101 78-79 -0.007863377570803465 1101 80-81 0.07669542582195987 1101 82-83 -0.16903001684999097 1101 84-85 -0.5971897700895035 1101 86-87 -0.9316695637998436 1101 88-89 -1.090205241026375 1101 90-91 -4.131133732388388 1101 92-93 -9.33429595119642 1101 94-95 -7.176781150735522 1101 96-97 -3.7115881202148007 1101 98-99 -2.700675845894146 1101 100-101 -2.1266775556642727 1102 1 0.895530464311669 1102 2 0.7556702658067955 1102 3 0.6780502673301001 1102 4 0.9533235628235346 1102 5 0.8360532676517423 1102 6 0.7884373069087687 1102 7 0.8384502765902226 1102 8 0.6846002301270175 1102 9 0.8756586084886706 1102 10-11 0.6850305696511825 1102 12-13 0.5656602437437428 1102 14-15 0.8135356424679898 1102 16-17 0.931023450490791 1102 18-19 0.8154327146565521 1102 20-21 0.7932481763530745 1102 22-23 1.046965589535347 1102 24-25 0.9230593782838383 1102 26-27 0.7949233583297826 1102 28-29 0.9135975415502386 1102 30-31 1.1103224160503942 1102 32-33 1.031175015221919 1102 34-35 0.9246530025506843 1102 36-37 0.8906084601611539 1102 38-39 0.7504979816732771 1102 40-41 1.009286856851773 1102 42-43 0.8109973667128969 1102 44-45 0.5746782888307891 1102 46-47 0.3503975982504741 1102 48-49 0.48152722265140113 1102 50-51 0.5762764194384609 1102 52-53 0.4476056830563664 1102 54-55 0.4851816253225003 1102 56-57 0.5242494708750165 1102 58-59 0.3723810224547073 1102 60-61 0.29889813889151284 1102 62-63 0.16148698081246593 1102 64-65 0.03809730162866032 1102 66-67 0.04248366370433487 1102 68-69 0.07096705766094402 1102 70-71 0.06716893398865764 1102 72-73 -0.009468974771397853 1102 74-75 -0.20439900666313093 1102 76-77 -0.25466884076803 1102 78-79 -0.29160448848797316 1102 80-81 -0.6468161274354607 1102 82-83 -0.6155447709706472 1102 84-85 -0.7646479627805647 1102 86-87 -1.0350932066633014 1102 88-89 -1.4118866123197087 1102 90-91 -5.162575356305094 1102 92-93 -10.406947555821805 1102 94-95 -8.953379258780526 1102 96-97 -5.213990184454222 1102 98-99 -3.676014237437702 1102 100-101 -3.0876369985508525 1103 1 0.8728566152354738 1103 2 0.874799373020057 1103 3 0.8301904035404988 1103 4 0.9116691293680077 1103 5 0.8787404171411168 1103 6 0.7194783461864631 1103 7 0.7807666083232192 1103 8 0.7115216831249214 1103 9 0.7822770320308692 1103 10-11 0.708441207558387 1103 12-13 0.5718714528137454 1103 14-15 0.8573837819480374 1103 16-17 0.876704847341756 1103 18-19 0.7377797855364747 1103 20-21 0.9323267382680314 1103 22-23 0.9617821286503911 1103 24-25 0.7068760516146924 1103 26-27 0.5936925108781566 1103 28-29 0.8847474354358624 1103 30-31 0.9997642460828544 1103 32-33 0.8586332615246945 1103 34-35 0.8713629211916754 1103 36-37 1.0318093094489242 1103 38-39 1.0080287464517 1103 40-41 0.7788089647109402 1103 42-43 0.8651774433091148 1103 44-45 0.8539246344793483 1103 46-47 0.8613985172558927 1103 48-49 0.6309321293574399 1103 50-51 0.5739655076005299 1103 52-53 0.8448813119832295 1103 54-55 0.9763285086731237 1103 56-57 0.6916184403679573 1103 58-59 0.7820627585733924 1103 60-61 0.5047142268923359 1103 62-63 0.6509440499308035 1103 64-65 0.5069558568141872 1103 66-67 0.47907077488628147 1103 68-69 0.6104717081452868 1103 70-71 0.7230276168656111 1103 72-73 0.6919240501079251 1103 74-75 0.6214261642057046 1103 76-77 0.39144265248529564 1103 78-79 0.07690783187023698 1103 80-81 0.07041576018863083 1103 82-83 0.02747428301300303 1103 84-85 -0.3024024485243295 1103 86-87 -0.7046954944834987 1103 88-89 -1.0018402100022499 1103 90-91 -2.474291906511592 1103 92-93 -9.278721017256665 1103 94-95 -5.4914457496617715 1103 96-97 -2.979388302077595 1103 98-99 -1.9156437365797458 1103 100-101 -1.3537855362976483 1104 1 0.7965098564307311 1104 2 0.7678947618776384 1104 3 0.6574478606595235 1104 4 0.709031627423542 1104 5 0.7993264053286637 1104 6 0.6715744637748244 1104 7 0.7807941481931024 1104 8 0.6996754511277672 1104 9 0.7749165714953961 1104 10-11 0.597091704448637 1104 12-13 0.5139343439794075 1104 14-15 0.8272700100121497 1104 16-17 0.7398906465799797 1104 18-19 0.74094321293353 1104 20-21 0.755966360137009 1104 22-23 0.7823196423638521 1104 24-25 0.7117091912007396 1104 26-27 0.705709102789136 1104 28-29 0.7375909377585756 1104 30-31 0.7704609423957649 1104 32-33 0.8248867010500405 1104 34-35 0.7768408164657288 1104 36-37 0.5859072958756357 1104 38-39 0.5796647889982154 1104 40-41 0.5426566244782265 1104 42-43 0.5711744866458943 1104 44-45 0.45979929751936055 1104 46-47 0.23356543382536188 1104 48-49 0.19955191068657285 1104 50-51 0.3533002562249905 1104 52-53 0.48543275914960304 1104 54-55 0.561793546270426 1104 56-57 0.5462254625614271 1104 58-59 0.30985382218683455 1104 60-61 0.33607856710698414 1104 62-63 0.3875933987527347 1104 64-65 0.27904138015885493 1104 66-67 0.2345466369433744 1104 68-69 0.13631658509478228 1104 70-71 0.2448836233650198 1104 72-73 0.43903424637898425 1104 74-75 -0.05736245588975564 1104 76-77 -0.14755692301342904 1104 78-79 -0.16179044023959932 1104 80-81 -0.21536342556499477 1104 82-83 -0.19680708237071798 1104 84-85 -0.44746957372982976 1104 86-87 -0.5895260177819033 1104 88-89 -0.5449276273584331 1104 90-91 -0.9430678259936158 1104 92-93 -4.162350544042091 1104 94-95 -1.8586155783573268 1104 96-97 -1.0071765686730032 1104 98-99 -0.7474157959617287 1104 100-101 -0.6861697123187263 1105 1 0.9778029319973278 1105 2 0.9020440213926975 1105 3 0.9133493333009888 1105 4 0.762605155823131 1105 5 0.7118413225849523 1105 6 0.7856697093909091 1105 7 0.8292901456971649 1105 8 0.832726049727512 1105 9 1.1164508087427194 1105 10-11 0.8517516816253163 1105 12-13 0.6177767875462763 1105 14-15 0.9578693347197458 1105 16-17 1.1087187969826005 1105 18-19 0.8017057207198377 1105 20-21 0.6203618927383516 1105 22-23 0.6993313666638343 1105 24-25 0.7461517362189767 1105 26-27 0.7421818618455944 1105 28-29 0.904922043994894 1105 30-31 0.999088783524904 1105 32-33 1.00253059121588 1105 34-35 0.9108835973551592 1105 36-37 0.9115002696240779 1105 38-39 0.8720792603388503 1105 40-41 0.6880857139209198 1105 42-43 0.9396525629450068 1105 44-45 1.0652121659629117 1105 46-47 0.9915092031859771 1105 48-49 0.8351886345280519 1105 50-51 0.7943818676144723 1105 52-53 0.7980244445821825 1105 54-55 0.7879802543289038 1105 56-57 0.7358005686139215 1105 58-59 0.7150021877992678 1105 60-61 0.7055864761925754 1105 62-63 0.7588940756202049 1105 64-65 0.8362188917868174 1105 66-67 0.7207451946596457 1105 68-69 0.7225105470770572 1105 70-71 0.9490374548802301 1105 72-73 0.7886236402016706 1105 74-75 0.7636399621567662 1105 76-77 0.591876826460382 1105 78-79 0.41658548778042004 1105 80-81 0.5193566758950041 1105 82-83 0.5477953575109318 1105 84-85 0.16572583580077094 1105 86-87 -0.10132195079465944 1105 88-89 -0.15709741146465817 1105 90-91 -1.4430192795077694 1105 92-93 -7.23937623246902 1105 94-95 -2.8829915240698405 1105 96-97 -1.1882014518755533 1105 98-99 -0.9031584245014947 1105 100-101 -0.9386101441623733 1106 1 0.7499116677278757 1106 2 0.7633880043195376 1106 3 0.617203736031005 1106 4 0.62376160870555 1106 5 0.6554244934802895 1106 6 0.6216812887108389 1106 7 0.7018317852908282 1106 8 0.6916711007832248 1106 9 0.702504648354342 1106 10-11 0.7096534317296488 1106 12-13 0.8059209268362011 1106 14-15 0.7502365977847774 1106 16-17 0.7595480891755386 1106 18-19 0.5406893210853951 1106 20-21 0.5901097968920084 1106 22-23 0.631195230416651 1106 24-25 0.3587863088353629 1106 26-27 0.5761745217611818 1106 28-29 0.7478299746668924 1106 30-31 0.7076145992806033 1106 32-33 0.7278819459223556 1106 34-35 0.6080060676805061 1106 36-37 0.8580961370957993 1106 38-39 0.7725803749540461 1106 40-41 0.7964747780139376 1106 42-43 0.6116876072918913 1106 44-45 0.4817550231812433 1106 46-47 0.6388457483014776 1106 48-49 0.20514711379146178 1106 50-51 0.185897717865128 1106 52-53 0.2643954873020604 1106 54-55 0.3881067710771795 1106 56-57 0.5604210183690803 1106 58-59 0.5140706785257727 1106 60-61 0.4471872432168702 1106 62-63 0.4072385947245003 1106 64-65 0.5587249402910679 1106 66-67 0.503756755490695 1106 68-69 0.5504748424131058 1106 70-71 0.5231861080975619 1106 72-73 0.49572086000273075 1106 74-75 0.6518595096546917 1106 76-77 0.41547741651970327 1106 78-79 0.34516757575578083 1106 80-81 0.29445664789756165 1106 82-83 0.20076011506892044 1106 84-85 0.20000652601698832 1106 86-87 -0.06737703180235499 1106 88-89 -0.10712296573479563 1106 90-91 -0.46469847899219374 1106 92-93 -3.975979059682535 1106 94-95 -1.4499387594543869 1106 96-97 -0.6800798822226675 1106 98-99 -0.5355070930381807 1106 100-101 -0.44568114937623626 1107 1 0.6833701828789316 1107 2 0.7176991748962358 1107 3 0.6072284683091596 1107 4 0.713575761853086 1107 5 0.5277802176343016 1107 6 0.6044371132130237 1107 7 0.5029434707854534 1107 8 0.623464114928268 1107 9 0.6131174817713045 1107 10-11 0.5226048084762382 1107 12-13 0.63683804836392 1107 14-15 0.8561394019588562 1107 16-17 0.740975757398104 1107 18-19 0.800889168535889 1107 20-21 0.7314648393879395 1107 22-23 0.6851735541966306 1107 24-25 0.8184902437002748 1107 26-27 0.8495716750151487 1107 28-29 0.7766015203835863 1107 30-31 0.8040555965793033 1107 32-33 0.6543145048515768 1107 34-35 0.6093662162768965 1107 36-37 0.6531726779217948 1107 38-39 0.6035649654270543 1107 40-41 0.5582663224129405 1107 42-43 0.63165461402739 1107 44-45 0.47267965815206736 1107 46-47 0.4507372607715112 1107 48-49 0.4909455659968316 1107 50-51 0.2972138754421785 1107 52-53 0.3696011123749159 1107 54-55 0.4833805239469946 1107 56-57 0.3591171255693162 1107 58-59 0.3147328878074731 1107 60-61 0.4665663515371534 1107 62-63 0.49982277569585065 1107 64-65 0.3902239418773803 1107 66-67 0.16599866301832478 1107 68-69 0.2438535098095791 1107 70-71 0.20538171862499865 1107 72-73 0.25731854996740466 1107 74-75 0.25433438459957713 1107 76-77 0.22656722053667266 1107 78-79 0.09047515812491014 1107 80-81 -0.031326498676662595 1107 82-83 0.11672960263436494 1107 84-85 -0.1672757235053446 1107 86-87 -0.17256080073800817 1107 88-89 -0.1104333786320737 1107 90-91 -0.33321670891115573 1107 92-93 -3.9231093805432984 1107 94-95 -1.4046392226777904 1107 96-97 -0.47034997339759954 1107 98-99 -0.1997157361584989 1107 100-101 -0.13393566124316791 1108 1 0.38656793989867566 1108 2 0.4089356952565275 1108 3 0.10908695469489871 1108 4 -0.0833313972836649 1108 5 -0.977263308661307 1108 6 -0.19073435913023928 1108 7 0.258320920567769 1108 8 0.05617430987758354 1108 9 0.210037358017928 1108 10-11 0.3053921009041929 1108 12-13 0.16722617853148591 1108 14-15 0.3425240468434936 1108 16-17 0.2573485396036901 1108 18-19 0.265756761564532 1108 20-21 0.3388424521082314 1108 22-23 0.1610765186741716 1108 24-25 0.1530291193090605 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56-57 0.46270670655501434 2311 58-59 0.43831035932547024 2311 60-61 0.4377782727226709 2311 62-63 0.32320872352691055 2311 64-65 0.3614619915379862 2311 66-67 0.4669291510589524 2311 68-69 0.47625441020075954 2311 70-71 0.4802258711115641 2311 72-73 0.49307499486029016 2311 74-75 0.7830720727835327 2311 76-77 0.7798189190840077 2311 78-79 0.6818669650209408 2311 80-81 0.724505160862627 2311 82-83 0.6924106324970474 2311 84-85 0.7211497942313692 2311 86-87 0.9806840808312423 2311 88-89 1.0576710417558388 2311 90-91 1.807375409483452 2311 92-93 2.6237995815629453 2311 94-95 1.9911915567444431 2311 96-97 1.4110899098925884 2311 98-99 1.2666174290096102 2311 100-101 1.2426703559285421 2312 1 0.33921082724812734 2312 2 0.04796770801535999 2312 3 0.1046187085981174 2312 4 0.06055875691084367 2312 5 0.14728189245907686 2312 6 0.17564740833208248 2312 7 0.24096052718309835 2312 8 -0.4081741609779286 2312 9 0.003998341934746463 2312 10-11 0.07398419186965555 2312 12-13 0.19644291748176812 2312 14-15 -0.3088686532386191 2312 16-17 0.057089589173116906 2312 18-19 0.17411296205720816 2312 20-21 0.2936664587379738 2312 22-23 0.1092370319099345 2312 24-25 0.17957784389995624 2312 26-27 0.08357618095494246 2312 28-29 0.11805461315418597 2312 30-31 0.3361091078667755 2312 32-33 0.2513561881927018 2312 34-35 0.07501546402805559 2312 36-37 0.17038674268297882 2312 38-39 0.3091670606133263 2312 40-41 0.4039336125637334 2312 42-43 0.28513510592549096 2312 44-45 0.2294713342603174 2312 46-47 0.6214335649727332 2312 48-49 0.20783115112533324 2312 50-51 0.2071364379755387 2312 52-53 0.1153122075769204 2312 54-55 0.19674607418678036 2312 56-57 0.18399012295907013 2312 58-59 0.1642688474633296 2312 60-61 0.38220725962984403 2312 62-63 0.3117387204820652 2312 64-65 0.5009958680433435 2312 66-67 0.4102744437763768 2312 68-69 0.10012187477633461 2312 70-71 0.25091139955685193 2312 72-73 0.3000309991260721 2312 74-75 0.3345943256375108 2312 76-77 0.5626678836123347 2312 78-79 0.5667965473974483 2312 80-81 0.46096732681107966 2312 82-83 0.719441865388557 2312 84-85 0.6572988325558988 2312 86-87 0.5380688755143161 2312 88-89 0.8092584287554097 2312 90-91 1.2601601848175186 2312 92-93 2.1978604236795753 2312 94-95 1.6835717429148573 2312 96-97 1.1303590237832246 2312 98-99 1.3048549597767725 2312 100-101 0.9489959801588945 2313 1 -0.2616323854960818 2313 2 -1.1823145076433335 2313 3 -0.49926459244015575 2313 4 -0.22287290021485973 2313 5 -0.6753249071724952 2313 6 -0.40688518828289233 2313 7 -1.2575883161890005 2313 8 -0.979505112112605 2313 9 -1.0687878234594876 2313 10-11 -2.092979604096392 2313 12-13 -0.533617696157556 2313 14-15 -0.17005744340409024 2313 16-17 -1.4907064528654246 2313 18-19 -1.1197654366242773 2313 20-21 -1.4013711684180663 2313 22-23 -0.9912822158346657 2313 24-25 -0.3018297190090067 2313 26-27 -0.8808295364693777 2313 28-29 -2.2268770913720246 2313 30-31 -0.6410327775523399 2313 32-33 -0.40853995119211106 2313 34-35 -0.10272706153779154 2313 36-37 -0.21859322774057688 2313 38-39 -0.35914663562719795 2313 40-41 -0.3729948598744315 2313 42-43 -0.10051981979956537 2313 44-45 -0.7278823115770479 2313 46-47 -0.7750504418721178 2313 48-49 -0.42002980195332285 2313 50-51 -0.33912683370761343 2313 52-53 -0.18296245055839222 2313 54-55 0.03755845261921209 2313 56-57 -0.3284867254858064 2313 58-59 -0.16454502196429743 2313 60-61 -0.24370123487446094 2313 62-63 -0.40440089672096136 2313 64-65 -0.419874873887224 2313 66-67 -0.26134908104397425 2313 68-69 -0.2859599322419655 2313 70-71 -0.430664757947131 2313 72-73 -0.3515767820834803 2313 74-75 -0.3507773773984475 2313 76-77 -0.3432775058299278 2313 78-79 -0.33796429026002883 2313 80-81 -0.012300811447921944 2313 82-83 0.016669654558441493 2313 84-85 0.1904223863329797 2313 86-87 0.17080571855147753 2313 88-89 0.448791435928932 2313 90-91 1.0404615635638699 2313 92-93 2.2358492622415405 2313 94-95 1.622371038067353 2313 96-97 1.0513000365164409 2313 98-99 1.0700526346800636 2313 100-101 1.1261352096736665 2314 1 -0.5415233297623736 2314 2 -0.56564496992657 2314 3 -1.690887309955329 2314 4 -0.48784984347407345 2314 5 -0.2914412156204449 2314 6 -3.094586713914744 2314 7 -3.882167596924699 2314 8 -1.3348936314458157 2314 9 -1.9572409016668928 2314 10-11 -1.0811613680695729 2314 12-13 -1.9816384383902559 2314 14-15 -2.8274594522557663 2314 16-17 -2.0898090288599036 2314 18-19 -2.5979333091319887 2314 20-21 -1.974202531769329 2314 22-23 -3.0065103759774203 2314 24-25 -1.9639915976475137 2314 26-27 -1.767646668246158 2314 28-29 -1.0139296492568164 2314 30-31 -1.2210506001479473 2314 32-33 -2.259928876889333 2314 34-35 -0.6911222770432275 2314 36-37 -0.2909298870750163 2314 38-39 -0.26201072894958344 2314 40-41 -0.3770237161364136 2314 42-43 -0.5420514366866414 2314 44-45 -1.106354073695087 2314 46-47 -1.4353052806957827 2314 48-49 -1.5533425962359573 2314 50-51 -0.9203781035951586 2314 52-53 -0.4526495723527546 2314 54-55 -0.11997993111209126 2314 56-57 -0.27872093691877 2314 58-59 -0.5849992662597394 2314 60-61 -0.44784007444018314 2314 62-63 -0.5870565518062563 2314 64-65 -0.5245649550716891 2314 66-67 -0.5314955708539522 2314 68-69 -0.40046679626020065 2314 70-71 -0.5407159730638718 2314 72-73 -0.2461562394991823 2314 74-75 -0.21991160119900854 2314 76-77 -0.20478301824611478 2314 78-79 -0.16592599928572582 2314 80-81 -0.19662167558983512 2314 82-83 -0.05542738251785906 2314 84-85 0.06420495066658916 2314 86-87 0.09568910974751077 2314 88-89 0.3693119352266301 2314 90-91 0.9593212708050984 2314 92-93 1.658002947344766 2314 94-95 1.0520811061774928 2314 96-97 1.0145897961432944 2314 98-99 1.009687702127657 2314 100-101 1.29707918886076 2315 1 -7.360366881239859 2315 2 -2.9550541758142863 2315 3 -1.359654508756801 2315 4 -0.7908616064540297 2315 5 -1.234745741655928 2315 6 -1.518753613973871 2315 7 -2.8529101850470227 2315 8 -2.7010925129402388 2315 9 -3.1821112021430125 2315 10-11 -2.034319779311531 2315 12-13 -2.593656816379351 2315 14-15 -4.2571983194354495 2315 16-17 -3.937570183908072 2315 18-19 -2.7307775185539356 2315 20-21 -4.20694621572482 2315 22-23 -5.4467060588837555 2315 24-25 -3.296022100930564 2315 26-27 -4.4433675730976105 2315 28-29 -2.734594162044502 2315 30-31 -2.34929225470043 2315 32-33 -1.425824792743569 2315 34-35 -2.0686434713013284 2315 36-37 -2.7386446970491 2315 38-39 -1.766183730554431 2315 40-41 -3.042873231346938 2315 42-43 -7.248305076497612 2315 44-45 -3.712339256608093 2315 46-47 -2.2602523228353455 2315 48-49 -5.17590753490931 2315 50-51 -6.263504751234784 2315 52-53 -6.467709776493358 2315 54-55 -4.198264485421404 2315 56-57 -3.440437182812172 2315 58-59 -3.7035893090705834 2315 60-61 -2.7041747347763554 2315 62-63 -2.9969217732179025 2315 64-65 -1.7347934907451688 2315 66-67 -2.2715916729776033 2315 68-69 -1.7847438686741022 2315 70-71 -1.2900860912715935 2315 72-73 -1.892198792501496 2315 74-75 -2.495068529843156 2315 76-77 -2.430691081622175 2315 78-79 -1.5211195927738537 2315 80-81 -1.3669784437488772 2315 82-83 -1.6307450741108376 2315 84-85 -1.6583639334507225 2315 86-87 -1.9742691685718832 2315 88-89 -1.3398332704476985 2315 90-91 -0.5963372738039787 2315 92-93 0.05837381907671002 2315 94-95 0.052044430219584825 2315 96-97 -0.08848159305308201 2315 98-99 -0.2749555185993735 2315 100-101 0.018607809444642243 2316 1 -4.337868912669077 2316 2 -3.045106479616358 2316 3 -4.364616182947824 2316 4 -4.515220276183239 2316 5 -4.389317410772048 2316 6 -4.546040181899876 2316 7 -2.962777504117817 2316 8 -1.5808231028907542 2316 9 -2.006589850868565 2316 10-11 -3.0232397630014276 2316 12-13 -3.7536105261944535 2316 14-15 -2.9042004579363834 2316 16-17 -2.9643037924010684 2316 18-19 -3.2033293163321233 2316 20-21 -5.896657063410167 2316 22-23 -6.453731137134113 2316 24-25 -4.343451646271774 2316 26-27 -4.187732520456457 2316 28-29 -4.572366718242485 2316 30-31 -6.525750263518454 2316 32-33 -4.810679864569629 2316 34-35 -6.198580724339671 2316 36-37 -9.53428233114543 2316 38-39 -6.43892151044431 2316 40-41 -5.647213596212158 2316 42-43 -4.627522308884313 2316 44-45 -3.7816322605712145 2316 46-47 -5.742884996430874 2316 48-49 -3.1923980465947963 2316 50-51 -4.48494931008463 2316 52-53 -5.175765090037494 2316 54-55 -6.930065666437148 2316 56-57 -5.095549197432597 2316 58-59 -5.951654244402199 2316 60-61 -6.392366630767182 2316 62-63 -6.6163196487976315 2316 64-65 -5.383343328964202 2316 66-67 -6.822288812549257 2316 68-69 -5.476004176160615 2316 70-71 -6.257550662266013 2316 72-73 -8.09749106896361 2316 74-75 -7.689170198108563 2316 76-77 -7.024830298326535 2316 78-79 -6.284512798266487 2316 80-81 -6.275510739806585 2316 82-83 -5.310246853261479 2316 84-85 -6.7795028245397155 2316 86-87 -5.467963670952397 2316 88-89 -5.3645228357951815 2316 90-91 -5.852615050115023 2316 92-93 -4.716923414952735 2316 94-95 -4.634840604087369 2316 96-97 -5.18857341072021 2316 98-99 -6.359033807094589 2316 100-101 -5.2759790550553625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9748.0 3 3755.0 4 3872.0 5 4305.0 6 5068.0 7 6149.0 8 7623.0 9 9677.0 10 11882.0 11 15044.0 12 18636.0 13 21875.0 14 23992.0 15 27790.0 16 31545.0 17 35588.0 18 30595.0 19 26827.0 20 27642.0 21 29874.0 22 32950.0 23 36728.0 24 40903.0 25 46666.0 26 53105.0 27 59967.0 28 68703.0 29 78861.0 30 89894.0 31 103857.0 32 119498.0 33 139701.0 34 165392.0 35 199332.0 36 232470.0 37 217705.0 38 74682.0 39 1442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07054424146328 20.12677087566054 14.117799165153544 21.684885717722633 2 14.674983102995705 21.85802158932725 41.36344023481034 22.103555072866705 3 18.608152673534526 27.730712742039454 31.161547821070663 22.49958676335536 4 11.513153064912073 17.782509911984516 40.379040160884145 30.32529686221927 5 12.063423351057759 39.28125380375761 35.017777312578055 13.637545532606579 6 27.969628434180414 38.77451091815551 19.21043210437787 14.045428543286206 7 25.566576933787744 35.144488247897655 22.71919986814761 16.569734950166996 8 24.827732529118528 34.63861954912571 21.544492129448525 18.989155792307233 9 26.785458054977678 16.61860804098393 21.879168823345836 34.71676508069256 10-11 23.475145043583524 27.395708448011753 29.05470252360799 20.074443984796737 12-13 25.706039835550605 25.527969989547866 28.86500363953927 19.900986535362257 14-15 22.629407160863074 26.263949521823715 28.609399180404544 22.497244136908673 16-17 20.336421961059564 29.5150430591599 28.981311706545554 21.16722327323498 18-19 20.08150019465702 28.262494281062 31.92931629445024 19.726689229830736 20-21 21.67624253390236 27.015550324221653 31.501698078353176 19.806509063522814 22-23 20.840782289626787 27.342335556888237 31.20714777687143 20.609734376613545 24-25 20.753220466188186 27.31784016026048 31.810359695323147 20.11857967822819 26-27 20.71906380266791 27.196395085887982 32.02571486446627 20.05882624697784 28-29 20.273906591157207 27.711441654536635 31.975977794456572 20.038673959849586 30-31 20.619155834002232 27.544590269887102 31.683588904293803 20.152664991816867 32-33 20.363238528623835 27.626706100978243 31.512347229158394 20.497708141239528 34-35 20.988104426335997 27.39691758381554 31.418964217572398 20.19601377227607 36-37 21.04976749959963 27.102643217308987 31.534412617849323 20.313176665242068 38-39 20.72270547880304 27.63614095931169 31.503093789679156 20.138059772206113 40-41 20.70223114160884 27.61948551183982 31.512376122700946 20.16590722385039 42-43 20.40979678583975 27.899302310762486 31.018188528409407 20.672712374988365 44-45 20.62931116567335 27.803853538442297 30.74199540205491 20.82483989382944 46-47 20.983963001236166 27.959443881401924 30.012551189093685 21.044041928268225 48-49 20.917222637797 27.966826786036275 30.069853684242204 21.04609689192452 50-51 20.828853695277818 28.241565622540744 30.095632225526842 20.8339484566546 52-53 20.92089594153132 28.65688159926251 29.581995855150666 20.840226604055506 54-55 20.67754573150502 28.127932570083093 29.84121702961813 21.353304668793758 56-57 21.01434167957045 28.62496043576569 29.197244054940747 21.163453829723117 58-59 21.132489986030546 28.196030988168957 29.387094611609122 21.284384414191372 60-61 21.000462865797818 28.312700194195177 29.757912186256817 20.928924753750184 62-63 21.173205319103072 28.058415572811725 29.70503698348546 21.06334212459974 64-65 20.8514857207009 28.42622413447633 29.61351698172911 21.108773163093662 66-67 20.859514053616408 28.328735570106055 29.215575639482307 21.59617473679523 68-69 20.8355529994687 28.422583058939566 29.35213067815074 21.38973326344099 70-71 20.97134991068093 28.26815287786017 29.222437291285818 21.53805992017308 72-73 20.971296569885958 27.95490459557704 29.332082552483868 21.741716282053137 74-75 20.732029842554507 28.738033047486745 28.948732933214465 21.581204176744286 76-77 20.819456310633594 28.23532618162557 29.14709189822029 21.798125609520547 78-79 20.768793073743268 28.215637636554476 29.228517131621963 21.787052158080296 80-81 20.78220915238081 28.514003113725394 28.93178931611565 21.771998417778143 82-83 20.81774069620226 28.464172445126163 28.917601197893735 21.800485660777845 84-85 20.757369468367077 28.186572670590227 28.865218213414252 22.19083964762845 86-87 20.698571087361195 28.169914166669823 28.940291052330274 22.191223693638705 88-89 20.99331476534371 27.941664407287398 28.79909883946905 22.26592198789984 90-91 20.565181160975868 27.931337040515153 29.333022754265457 22.170459044243522 92-93 20.357773967145583 28.12168072663101 29.598298058559536 21.922247247663872 94-95 20.623917673638505 27.977918705514565 28.991546152754154 22.406617468092776 96-97 20.53715696761732 27.83863674233868 28.93912304782727 22.68508324221673 98-99 20.63868363956364 27.83805132834666 28.53413418501267 22.98913084707703 100-101 20.77781734502901 27.79418159092278 28.8886955309501 22.539305533098112 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3735.0 1 2430.5 2 1160.5 3 1134.0 4 988.5 5 980.0 6 1394.0 7 2523.0 8 3823.0 9 4217.0 10 3633.0 11 3046.5 12 3019.5 13 3221.0 14 3415.5 15 3630.5 16 3828.0 17 4143.5 18 4599.0 19 5087.0 20 5732.5 21 6614.5 22 7704.5 23 9074.0 24 10851.5 25 13393.5 26 17445.5 27 22481.0 28 26365.0 29 29815.5 30 34256.5 31 38948.5 32 44047.0 33 50020.5 34 56497.0 35 61491.5 36 66967.0 37 72807.5 38 73408.5 39 71556.5 40 71297.5 41 71381.0 42 71223.5 43 71864.5 44 73233.5 45 73673.0 46 73978.5 47 77869.5 48 89415.5 49 87582.0 50 71711.0 51 62340.0 52 56679.0 53 52465.5 54 48100.5 55 43686.0 56 39358.0 57 35048.5 58 31845.0 59 28721.5 60 24866.0 61 20650.5 62 16539.5 63 12396.5 64 8587.0 65 6004.5 66 4259.5 67 3081.0 68 2182.0 69 1553.5 70 1122.5 71 781.0 72 569.5 73 460.0 74 353.5 75 282.0 76 208.5 77 118.0 78 66.5 79 39.5 80 25.5 81 22.0 82 15.5 83 9.5 84 11.0 85 10.0 86 6.0 87 4.0 88 1.5 89 2.0 90 4.0 91 2.0 92 0.5 93 2.0 94 2.5 95 1.5 96 1.5 97 4.0 98 3.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0859775247084832 2 0.09567779579557129 3 0.092838692062765 4 0.09510997504901003 5 0.09198696094292313 6 0.07717630313678375 7 0.08947908597894426 8 0.08522043037973485 9 0.07977881489185618 10-11 0.08623777588399044 12-13 0.08574093273074933 14-15 0.09158475458077558 16-17 0.08931347159453056 18-19 0.09165573217409574 20-21 0.10093013770126288 22-23 0.1092581753174946 24-25 0.13149782122447704 26-27 0.13677382232794205 28-29 0.13622966077915416 30-31 0.13745993906337023 32-33 0.1338637410018156 34-35 0.12898994626049817 36-37 0.1329173730908802 38-39 0.1260088873410516 40-41 0.12231805248840344 42-43 0.12253098526836391 44-45 0.11356414931225078 46-47 0.13182904999330447 48-49 0.14888733158791545 50-51 0.15768855315961489 52-53 0.1654014516337386 54-55 0.1617815943744106 56-57 0.13490474570384456 58-59 0.1261745017254653 60-61 0.12208146051066958 62-63 0.11999945110661166 64-65 0.10788594184663823 66-67 0.11990481431551811 68-69 0.11723132496712554 70-71 0.12546472579226373 72-73 0.15115861457416047 74-75 0.16419483254729592 76-77 0.16989669921068185 78-79 0.1663951379402208 80-81 0.17278312133903487 82-83 0.172238959790247 84-85 0.15066177142091938 86-87 0.1517500945184951 88-89 0.1712452734837648 90-91 0.2580035517187697 92-93 0.52230045004526 94-95 0.17003865439732216 96-97 0.18295657638159069 98-99 0.2013397730515113 100-101 0.20895803473454144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2113343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.01248499456581 #Duplication Level Percentage of deduplicated Percentage of total 1 75.80727183519205 40.94539132477283 2 12.952301240135741 13.991719527558558 3 4.765023551679888 7.721122892515879 4 2.245305634279692 4.850981475189839 5 1.242461651274761 3.355422064790074 6 0.7429743255987361 2.4077933767649635 7 0.5005481408541232 1.8925094262858804 8 0.3387817391924083 1.463875488365029 9 0.22267665660256394 1.082458761604746 >10 1.0519538093842975 9.982968919452986 >50 0.07514133530647897 2.8119088058037747 >100 0.05056789489665223 4.916895596698621 >500 0.0026743851442714538 0.9861614298894464 >1k 0.00196121577246573 1.8856045972316084 >5k 2.6743851442714503E-4 1.0346003927559453 >10k+ 8.9146171475715E-5 0.6705859203198236 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13927 0.659003294779882 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9393 0.44446168937082153 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6806 0.32204900009132453 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5288 0.25021967565132586 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4113 0.19462056088386975 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3181 0.15051981623427907 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2878 0.1361823423836074 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2183 0.10329605747860145 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2165 0.10244432635875957 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 0.10003108818587422 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.097759332015674E-5 0.0 14-15 0.0 0.0 0.0 9.463679109354231E-5 0.0 16-17 0.0 0.0 0.0 2.1293277996047022E-4 0.0 18-19 0.0 0.0 0.0 3.075695710540125E-4 0.0 20-21 0.0 0.0 0.0 4.731839554677116E-4 0.0 22-23 0.0 0.0 0.0 8.753903176152664E-4 0.0 24-25 0.0 0.0 0.0 0.001916395019644232 0.0 26-27 0.0 0.0 0.0 0.002744466941712727 0.0 28-29 0.0 0.0 0.0 0.006387983398814107 0.0 30-31 0.0 0.0 0.0 0.017294873572344858 0.0 32-33 0.0 0.0 0.0 0.04083577535686351 4.7318395546771155E-5 34-35 0.0 0.0 0.0 0.07246812277988003 4.7318395546771155E-5 36-37 0.0 0.0 0.0 0.12250732607059053 4.7318395546771155E-5 38-39 0.0 0.0 0.0 0.19097704442676838 4.7318395546771155E-5 40-41 0.0 0.0 0.0 0.27113440648299875 4.7318395546771155E-5 42-43 0.0 0.0 0.0 0.3501324678483332 4.7318395546771155E-5 44-45 0.0 0.0 0.0 0.44557367166617057 4.7318395546771155E-5 46-47 0.0 0.0 0.0 0.555920170081241 4.7318395546771155E-5 48-49 0.0 0.0 0.0 0.6806514607425298 4.7318395546771155E-5 50-51 0.0 0.0 0.0 0.816336959972896 4.7318395546771155E-5 52-53 0.0 0.0 0.0 0.948568216328348 4.7318395546771155E-5 54-55 0.0 0.0 0.0 1.0941432602279895 4.7318395546771155E-5 56-57 0.0 0.0 0.0 1.259166164697354 4.7318395546771155E-5 58-59 0.0 0.0 0.0 1.4443703648674162 4.7318395546771155E-5 60-61 0.0 0.0 0.0 1.6582495127388217 4.7318395546771155E-5 62-63 0.0 0.0 0.0 1.8707091087438243 4.7318395546771155E-5 64-65 0.0 0.0 0.0 2.085392669339525 4.7318395546771155E-5 66-67 0.0 0.0 0.0 2.3290824064053965 4.7318395546771155E-5 68-69 4.7318395546771155E-5 0.0 0.0 2.5981111442865643 4.7318395546771155E-5 70-71 4.7318395546771155E-5 0.0 0.0 2.903149181178824 4.7318395546771155E-5 72-73 4.7318395546771155E-5 0.0 0.0 3.2202770681332846 4.7318395546771155E-5 74-75 4.7318395546771155E-5 0.0 0.0 3.5448812615841345 4.7318395546771155E-5 76-77 4.7318395546771155E-5 0.0 0.0 3.889808705922323 4.7318395546771155E-5 78-79 4.7318395546771155E-5 0.0 0.0 4.281463065862948 4.7318395546771155E-5 80-81 4.7318395546771155E-5 0.0 0.0 4.705648822741978 4.7318395546771155E-5 82-83 4.7318395546771155E-5 0.0 0.0 5.1265932695260545 4.7318395546771155E-5 84-85 4.7318395546771155E-5 0.0 0.0 5.555061341202068 4.7318395546771155E-5 86-87 4.7318395546771155E-5 0.0 0.0 6.016131787409805 4.7318395546771155E-5 88-89 4.7318395546771155E-5 4.7318395546771155E-5 0.0 6.503818831112602 4.7318395546771155E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11200 0.0 28.557564 1 TACATGG 11110 0.0 28.489906 2 ACATGGG 11190 0.0 27.226025 3 CATGGGA 8400 0.0 24.970215 4 GAGTACT 9870 0.0 23.339674 12-13 AGTACTT 10445 0.0 21.804972 12-13 GTATCAA 24335 0.0 21.275152 1 ATGGGAT 3265 0.0 21.220175 5 GTACTTT 11350 0.0 20.297464 14-15 ATGGGAG 3295 0.0 20.16285 5 CATGGGG 2855 0.0 19.945934 4 CATTCCG 455 0.0 19.813435 9 TCAACGC 27625 0.0 18.844456 4 CAACGCA 27615 0.0 18.834095 5 TATCAAC 27730 0.0 18.824442 2 ATCAACG 27640 0.0 18.765554 3 AACGCAG 28040 0.0 18.629278 6 AGTACAT 7785 0.0 18.286976 2 GTATAGC 950 0.0 17.982836 1 ACTTTTT 13535 0.0 17.95152 16-17 >>END_MODULE