FastQCFastQC Report
Fri 27 May 2016
SRR939673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113343
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139570.660422846646285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108300.5124582237715316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79090.37424119037941306No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56640.26801139237691185No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42920.2030905536867418No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34500.1632484646363605No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32510.15383210392255303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26630.1260088873410516No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26380.12482592745238232No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24020.11365878610334432No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21800.10315410229196113No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.10012572497696778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108950.025.1900371
TACATGG108950.024.705972
ACATGGG108100.023.767283
CATGGGA83850.022.2231034
GTATCAA216650.022.054381
GAGTACT105100.021.99932912-13
AGTACTT109000.021.66489412-13
GGTATCA163450.020.218071
GTACTTT117800.019.5874614-15
ATCAACG247100.019.195543
TCAACGC247800.019.1413154
AACGCAG249850.019.0155336
CAACGCA251850.018.8335045
CTTTGCG5500.018.8231852
GTGTAGC14150.018.6302681
TATCAAC257800.018.5092222
ATGGGAG32150.018.2953875
ATGGGAT37400.017.9918985
CGCAGAG265100.017.6739048
AGAGTAC201950.017.66220710-11
Produced by FastQC (version 0.11.5)