##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939673_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2113343 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.529530700884806 31.0 31.0 34.0 30.0 34.0 2 31.725503621513404 31.0 31.0 34.0 30.0 34.0 3 31.761296675456848 31.0 31.0 34.0 30.0 34.0 4 35.55495440162813 37.0 35.0 37.0 33.0 37.0 5 35.29892639292344 37.0 35.0 37.0 33.0 37.0 6 35.33311062141829 37.0 35.0 37.0 32.0 37.0 7 35.26742275153631 37.0 35.0 37.0 32.0 37.0 8 35.23630664780871 37.0 35.0 37.0 32.0 37.0 9 36.82540931595108 39.0 37.0 39.0 32.0 39.0 10-11 36.71124682552714 39.0 35.0 39.0 32.0 39.0 12-13 36.65879107177585 39.0 35.0 39.0 32.0 39.0 14-15 37.6532822641663 39.0 36.5 41.0 32.0 41.0 16-17 37.63695505178289 39.0 36.0 41.0 32.0 41.0 18-19 37.685330540286174 39.0 36.5 41.0 32.0 41.0 20-21 37.64326212072531 39.0 36.5 41.0 32.0 41.0 22-23 37.50411244175697 39.0 36.0 41.0 32.0 41.0 24-25 37.37466114113989 39.0 36.0 41.0 31.5 41.0 26-27 37.09708528147111 39.0 36.0 40.5 31.0 41.0 28-29 36.896665141437055 39.0 36.0 40.0 31.0 41.0 30-31 36.625592248868266 38.5 35.0 40.0 30.0 41.0 32-33 36.394303007131356 38.0 35.0 40.0 30.0 41.0 34-35 36.33359989362825 38.0 35.0 40.0 30.0 41.0 36-37 36.166687092440746 38.0 35.0 40.0 30.0 41.0 38-39 36.14924813435396 38.0 35.0 40.0 30.0 41.0 40-41 36.034175001407725 38.0 35.0 40.0 30.0 41.0 42-43 35.806147889859815 38.0 35.0 40.0 29.0 41.0 44-45 35.526162814081765 38.0 35.0 40.0 27.5 41.0 46-47 35.123519466551336 38.0 34.0 40.0 26.5 41.0 48-49 34.758064355857044 38.0 34.0 40.0 24.5 41.0 50-51 34.493761306139135 38.0 33.5 40.0 24.0 41.0 52-53 34.2591401395798 38.0 33.0 40.0 23.5 41.0 54-55 33.996398833506916 38.0 33.0 40.0 23.0 41.0 56-57 32.02727739888887 37.0 30.0 39.5 14.0 41.0 58-59 27.126175211501398 32.5 17.0 38.5 4.0 40.0 60-61 27.863492816830963 33.0 19.0 38.0 4.0 40.0 62-63 30.91355142066385 34.5 29.0 38.5 2.0 40.0 64-65 31.150703884792954 35.5 30.5 39.0 2.0 40.0 66-67 24.554057244848565 27.0 17.5 35.5 2.0 39.0 68-69 17.195879939981346 13.0 7.0 28.0 2.0 35.5 70-71 16.09301660923002 12.5 7.0 26.5 2.0 33.5 72-73 17.222203400015992 13.5 7.0 28.5 2.0 34.0 74-75 18.208500939033563 17.5 7.5 29.5 2.0 34.5 76-77 19.35499466958274 21.0 8.0 30.0 2.0 34.5 78-79 22.37870236871156 27.5 10.0 33.5 2.0 36.0 80-81 25.93820714384745 31.5 22.5 34.5 2.0 36.0 82-83 27.20298716299247 33.0 26.0 35.0 2.0 36.5 84-85 27.232920306831403 34.0 26.0 35.0 2.0 36.0 86-87 26.985301250199328 33.5 26.0 35.0 2.0 36.0 88-89 26.545462804665405 33.0 25.5 35.0 2.0 35.5 90-91 25.19051356074239 32.5 13.0 35.0 2.0 35.0 92-93 22.778389736072185 31.5 2.0 34.5 2.0 35.0 94-95 15.626140195888695 4.5 2.0 32.0 2.0 35.0 96-97 13.106068678865665 2.0 2.0 30.0 2.0 34.0 98-99 12.789190869631668 2.0 2.0 29.5 2.0 34.0 100-101 12.5005408492611 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3748230737008882 1101 2 0.40308928727496607 1101 3 0.4566837846658096 1101 4 0.347808208287276 1101 5 0.40463532730898066 1101 6 0.5092955469647293 1101 7 0.3718838130412436 1101 8 0.4484299657031414 1101 9 0.4170971175891296 1101 10-11 0.497466590507301 1101 12-13 0.6044501911145659 1101 14-15 0.7141700381479339 1101 16-17 0.6690342306320005 1101 18-19 0.6077228243320505 1101 20-21 0.584922796563518 1101 22-23 0.6788460686281539 1101 24-25 0.7032786837950198 1101 26-27 0.6298317162679368 1101 28-29 0.7148289131123633 1101 30-31 0.7081658994597504 1101 32-33 0.7634566827270604 1101 34-35 0.6591539998407185 1101 36-37 0.42873985520517266 1101 38-39 0.5873687939380048 1101 40-41 0.5878696587221413 1101 42-43 0.6442796580193431 1101 44-45 0.4221994329897356 1101 46-47 0.4365605298251225 1101 48-49 0.40231081066604446 1101 50-51 0.3278893776828866 1101 52-53 0.24123406514255663 1101 54-55 0.09914218842347822 1101 56-57 -1.988794256271401 1101 58-59 -9.322186927851302 1101 60-61 -9.891238448473029 1101 62-63 -3.565695540782702 1101 64-65 -4.3559686775600674 1101 66-67 -9.301124377388941 1101 68-69 -4.552787792294573 1101 70-71 -4.1157758668199875 1101 72-73 -4.378500033630271 1101 74-75 -6.612579090593627 1101 76-77 -7.880573905272188 1101 78-79 -10.889183279368396 1101 80-81 -7.677357135808446 1101 82-83 -3.8710244213151483 1101 84-85 -2.8987510631262765 1101 86-87 -2.657197496441956 1101 88-89 -2.6959889261324577 1101 90-91 -9.207976874953523 1101 92-93 -16.575507221904513 1101 94-95 -10.712715623532784 1101 96-97 -8.968201684340851 1101 98-99 -8.967201430220474 1101 100-101 -8.877280028002327 1102 1 0.3731296991704198 1102 2 0.41163663488826074 1102 3 0.4655501984975281 1102 4 0.278666615427106 1102 5 0.32840967098466933 1102 6 0.3755580630880573 1102 7 0.41908124418981174 1102 8 0.46975186328005236 1102 9 0.525292868964577 1102 10-11 0.5091399936460519 1102 12-13 0.3894451812648896 1102 14-15 0.49072705862484867 1102 16-17 0.5272574681736799 1102 18-19 0.5624188216661778 1102 20-21 0.5767146339088853 1102 22-23 0.6284877283618826 1102 24-25 0.518326254697989 1102 26-27 0.5079889416726289 1102 28-29 0.5908405173750424 1102 30-31 0.588607593650373 1102 32-33 0.5751690765511199 1102 34-35 0.36122776024296144 1102 36-37 0.2671248814218785 1102 38-39 0.43993639857245626 1102 40-41 0.4936144941565104 1102 42-43 0.5301633297631128 1102 44-45 0.9104976935971294 1102 46-47 0.786385468448799 1102 48-49 0.5821822660840539 1102 50-51 0.657300028940675 1102 52-53 0.5075254275723111 1102 54-55 0.568492279439532 1102 56-57 -2.0227070848869353 1102 58-59 -9.666370118332136 1102 60-61 -9.687749008828117 1102 62-63 -3.4579149155262563 1102 64-65 -4.481654981628477 1102 66-67 -5.639669220077394 1102 68-69 -3.8841808478721678 1102 70-71 -4.009232119044382 1102 72-73 -4.160289910106872 1102 74-75 -5.000196374348338 1102 76-77 -7.075475476460792 1102 78-79 -9.738458494667253 1102 80-81 -5.227607798310061 1102 82-83 -2.601676143819347 1102 84-85 -2.091637359940602 1102 86-87 -2.0564853497100337 1102 88-89 -2.0254976208285953 1102 90-91 -8.141770634630355 1102 92-93 -12.648293423735716 1102 94-95 -10.077445998410088 1102 96-97 -8.918032346887806 1102 98-99 -8.891097053062666 1102 100-101 -8.731711308052924 1103 1 0.30836777843044416 1103 2 0.29594820568849656 1103 3 0.2708536333968006 1103 4 0.24029907104501547 1103 5 0.2528443102191176 1103 6 0.3893871840025156 1103 7 0.3246331647340597 1103 8 0.33098786965157245 1103 9 0.4685340314598321 1103 10-11 0.4890341868552852 1103 12-13 0.4604585059114754 1103 14-15 0.5539724107036577 1103 16-17 0.40908429903101506 1103 18-19 0.4544867143569107 1103 20-21 0.45646228489933094 1103 22-23 0.5926631773090847 1103 24-25 0.5486685795580186 1103 26-27 0.6417213573054212 1103 28-29 0.6663037017008762 1103 30-31 0.5454379896149462 1103 32-33 0.5115871364057725 1103 34-35 0.5183951636984503 1103 36-37 0.3990982777993608 1103 38-39 0.3350341663958929 1103 40-41 0.35827148889692495 1103 42-43 0.2442449907582258 1103 44-45 0.2819765094036484 1103 46-47 0.1755310217581041 1103 48-49 0.1597022609763954 1103 50-51 0.03246791520024317 1103 52-53 0.24935841930778935 1103 54-55 0.11170405892669777 1103 56-57 -2.688144372087315 1103 58-59 -10.276926773711626 1103 60-61 -10.20433794217599 1103 62-63 -4.068248809956209 1103 64-65 -5.027101594030949 1103 66-67 -6.4133025024273635 1103 68-69 -4.014297242798438 1103 70-71 -4.150810916303023 1103 72-73 -4.248384875243387 1103 74-75 -4.976426429553907 1103 76-77 -6.544383663531523 1103 78-79 -8.981300901082022 1103 80-81 -4.617566801906857 1103 82-83 -2.383056660531544 1103 84-85 -1.95912034296137 1103 86-87 -1.7575804667713761 1103 88-89 -1.7283771556217111 1103 90-91 -4.472784900651668 1103 92-93 -9.582673081093956 1103 94-95 -8.316960505367406 1103 96-97 -7.96762741594836 1103 98-99 -8.283146071656216 1103 100-101 -8.240118087255812 1104 1 0.16781181300416748 1104 2 0.22306544614189505 1104 3 0.16934920345812543 1104 4 0.0625429809147704 1104 5 0.19949613978088365 1104 6 0.06772516146155994 1104 7 0.16623781828356954 1104 8 0.1928212030924854 1104 9 0.2271838635204162 1104 10-11 0.2594029865924554 1104 12-13 0.25838471067858393 1104 14-15 0.37235708115145627 1104 16-17 0.3979177424162472 1104 18-19 0.3769079766855796 1104 20-21 0.24068320887487715 1104 22-23 0.5329833110840028 1104 24-25 0.5406003658821916 1104 26-27 0.46938588812608373 1104 28-29 0.4046580481700772 1104 30-31 0.49443296724633257 1104 32-33 0.4096500292926777 1104 34-35 0.39797127346377437 1104 36-37 0.34882273924966967 1104 38-39 0.26061261661984503 1104 40-41 0.19328464671522738 1104 42-43 0.2718248795100635 1104 44-45 0.2590856062314586 1104 46-47 0.14453909373699503 1104 48-49 0.1663704595483253 1104 50-51 0.20045208493878874 1104 52-53 0.23407400668049405 1104 54-55 0.10750293783883791 1104 56-57 -2.9048638916370706 1104 58-59 -10.09423147246525 1104 60-61 -10.211482924745187 1104 62-63 -4.1784237806466855 1104 64-65 -5.02271780614991 1104 66-67 -6.2179737161847335 1104 68-69 -3.774187982667799 1104 70-71 -3.8197693089385183 1104 72-73 -3.692702593255344 1104 74-75 -4.536589534433263 1104 76-77 -6.464061696634015 1104 78-79 -8.938986966789999 1104 80-81 -4.625294532425791 1104 82-83 -2.486166471244424 1104 84-85 -2.0453691910740375 1104 86-87 -1.9739650972003098 1104 88-89 -1.8347187055182523 1104 90-91 -4.079934788760188 1104 92-93 -6.194107570520906 1104 94-95 -7.405368436247497 1104 96-97 -7.7443110217865545 1104 98-99 -8.238885950700247 1104 100-101 -8.328605733149887 1105 1 0.26094058654948427 1105 2 0.2716117162290814 1105 3 0.334927026387728 1105 4 0.21296912196755358 1105 5 0.28266252714343665 1105 6 0.19541536497489886 1105 7 0.260538611878367 1105 8 0.28939418798719174 1105 9 0.27574782610466997 1105 10-11 0.37727439364007154 1105 12-13 0.23729169410135853 1105 14-15 0.37644577407076696 1105 16-17 0.43279591123417305 1105 18-19 0.3839879961818511 1105 20-21 0.36795321558292216 1105 22-23 0.4423213463063149 1105 24-25 0.5036519591454933 1105 26-27 0.5343555422571811 1105 28-29 0.6061736478820805 1105 30-31 0.6254837742723254 1105 32-33 0.5666527088429305 1105 34-35 0.48514177497917643 1105 36-37 0.44662697090659975 1105 38-39 0.47262321140491537 1105 40-41 0.3888517915755685 1105 42-43 0.4833184002802966 1105 44-45 0.5436767574369341 1105 46-47 0.5904161269986758 1105 48-49 0.3746081293342627 1105 50-51 0.5765468891055079 1105 52-53 0.5858277107234287 1105 54-55 0.4352453342185143 1105 56-57 1.6405017205783459 1105 58-59 -8.031076917499519 1105 60-61 -9.418393117845849 1105 62-63 -3.459262612139245 1105 64-65 -4.604055393449979 1105 66-67 -5.9444008911697885 1105 68-69 -3.931298003892323 1105 70-71 -3.8983634803826988 1105 72-73 -2.3904505541411165 1105 74-75 -4.3771380622556855 1105 76-77 -6.2905062836253265 1105 78-79 -8.895215297526637 1105 80-81 -4.231341741617776 1105 82-83 -1.9288032962783603 1105 84-85 -1.470387309701369 1105 86-87 -1.4078634469002829 1105 88-89 -1.5093371786859606 1105 90-91 -4.075134835882643 1105 92-93 -6.165450472042956 1105 94-95 -7.655794081047317 1105 96-97 -7.9412751187474795 1105 98-99 -8.336197489466342 1105 100-101 -8.211143950561762 1106 1 0.1479926934891509 1106 2 0.23269064788890859 1106 3 0.07556240987346641 1106 4 0.0317622736872849 1106 5 0.0057882832064706236 1106 6 0.0788951566316598 1106 7 0.06394241424811753 1106 8 0.0853113155551668 1106 9 0.11929626432276308 1106 10-11 0.20057751086483933 1106 12-13 0.2156494723721778 1106 14-15 0.25495535923318613 1106 16-17 0.21094885915363903 1106 18-19 -0.008071625165939622 1106 20-21 0.2013730504508331 1106 22-23 0.1683366498144494 1106 24-25 0.12246313752089577 1106 26-27 0.341713653327794 1106 28-29 0.17833474675531846 1106 30-31 0.24528410760037644 1106 32-33 0.10015818683324795 1106 34-35 0.35798550962680764 1106 36-37 0.28823836903551836 1106 38-39 0.2888843773663652 1106 40-41 0.22799426422523794 1106 42-43 0.09571756986557034 1106 44-45 0.12370487373726036 1106 46-47 0.1481525997434261 1106 48-49 -0.05322020671957972 1106 50-51 -0.0366463117418121 1106 52-53 0.12221184092738469 1106 54-55 -0.06986717060179615 1106 56-57 1.242911283554104 1106 58-59 -8.740625894536333 1106 60-61 -10.196075920020139 1106 62-63 -4.049829718841199 1106 64-65 -5.603253108224703 1106 66-67 -6.933867513463149 1106 68-69 -3.820954401308107 1106 70-71 -4.029896987511481 1106 72-73 -4.44602679963115 1106 74-75 -4.883656382092138 1106 76-77 -6.408147050015717 1106 78-79 -8.875520020642487 1106 80-81 -4.553779513338242 1106 82-83 -2.19068702236828 1106 84-85 -1.7814600343808493 1106 86-87 -1.691533673996524 1106 88-89 -1.6275808145501571 1106 90-91 -4.056071812581376 1106 92-93 -6.22989510577829 1106 94-95 -7.308603994255242 1106 96-97 -7.212138496506945 1106 98-99 -6.757083251974068 1106 100-101 -7.249277260651937 1107 1 0.08522250095241901 1107 2 0.07749623596470556 1107 3 0.04849120828005837 1107 4 0.041756546745823186 1107 5 -0.014150215523976328 1107 6 -0.0900477158602655 1107 7 0.027207632454718578 1107 8 0.16796425783469005 1107 9 0.09541162418247495 1107 10-11 0.12321692134462836 1107 12-13 0.019529918668162338 1107 14-15 0.15180199376816006 1107 16-17 0.10728710093193428 1107 18-19 0.1307658437286605 1107 20-21 0.10356335215421808 1107 22-23 0.2913005447428034 1107 24-25 0.36666809360590236 1107 26-27 0.3783651283953233 1107 28-29 0.34156429828004775 1107 30-31 0.42563493344560044 1107 32-33 0.4023211842029113 1107 34-35 0.37280175336137944 1107 36-37 0.3089722411067939 1107 38-39 0.24521613198972858 1107 40-41 0.3338600079927545 1107 42-43 0.24215749019519706 1107 44-45 0.15278842373142965 1107 46-47 0.390709793136665 1107 48-49 0.22376768814796577 1107 50-51 0.2606665237023762 1107 52-53 0.3457126999686082 1107 54-55 0.029876320703898784 1107 56-57 1.3833832114047802 1107 58-59 -8.768319204151002 1107 60-61 -9.925747495131766 1107 62-63 -3.53865521437368 1107 64-65 -5.119555252533491 1107 66-67 -6.741878136179341 1107 68-69 -3.7558630236492583 1107 70-71 -3.770513102516576 1107 72-73 -2.409444060531346 1107 74-75 -4.345217138352496 1107 76-77 -6.5741358596734685 1107 78-79 -7.31326170517417 1107 80-81 -2.36633082774733 1107 82-83 -1.2239482621244022 1107 84-85 -0.9721975934874472 1107 86-87 -0.8709649583011547 1107 88-89 -0.8407237650906723 1107 90-91 -0.27644914453618696 1107 92-93 -2.8533338173685046 1107 94-95 -5.633249988183671 1107 96-97 -6.246481139921215 1107 98-99 -5.867149175151963 1107 100-101 -6.450218151520759 1108 1 -0.05064571004827556 1108 2 -0.08440211061230585 1108 3 -0.11746667821576295 1108 4 -0.04656437747013342 1108 5 -0.07938759826328123 1108 6 -0.16258094427744396 1108 7 -0.09819439711652933 1108 8 -0.1181782652001715 1108 9 -0.07128186763783617 1108 10-11 -0.08712646049255568 1108 12-13 -0.04144415233702148 1108 14-15 -0.21232095286568153 1108 16-17 -0.15057356325886673 1108 18-19 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0.9740851952740321 2316 88-89 1.0318149662340659 2316 90-91 2.3031242815866264 2316 92-93 4.137648971777825 2316 94-95 10.918136177468877 2316 96-97 12.910324496180767 2316 98-99 12.436620090786258 2316 100-101 11.10499036400092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 16.0 4 32.0 5 99.0 6 242.0 7 609.0 8 1276.0 9 2305.0 10 4016.0 11 6390.0 12 9543.0 13 13186.0 14 16006.0 15 21482.0 16 28773.0 17 38590.0 18 39060.0 19 36138.0 20 36543.0 21 36236.0 22 35822.0 23 31272.0 24 31665.0 25 36805.0 26 51387.0 27 76883.0 28 107299.0 29 144635.0 30 178902.0 31 198177.0 32 198917.0 33 183286.0 34 163887.0 35 148646.0 36 121197.0 37 79804.0 38 31749.0 39 2459.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17564242975562 20.873299325362378 14.93266402819336 22.018394216688645 2 14.791670708779089 22.589390626116508 40.286034860439464 22.332903804664937 3 18.26935316042827 27.627870046282254 31.8122003258354 22.29057646745408 4 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20.784702458990186 28.114868937943154 30.009865331708458 21.090563271358207 50-51 20.719516579905154 28.409315386987622 29.98106547782311 20.890102555284116 52-53 20.888428744163267 28.715248982553387 29.519923387797647 20.876398885485692 54-55 20.57902945604939 28.244615698985378 29.775204145152312 21.40115069981292 56-57 20.19492030064527 28.60255333888921 30.000316413611117 21.20220994685441 58-59 20.09368783555432 27.95614057645586 30.831570267438853 21.118601320550976 60-61 20.84408797141345 28.35290830068196 29.85232265697927 20.950681070925317 62-63 21.064081946225215 28.25219290099395 29.58706289561595 21.096662257164887 64-65 20.46586105653128 28.500454206831698 29.90545154791556 21.128233188721456 66-67 20.867634423822462 28.106690729174527 29.509616281932598 21.516058565070413 68-69 21.30366650878669 27.773977564808916 29.6571163486136 21.265239577790794 70-71 21.219532014467077 27.846642749503808 29.491122054897232 21.44270318113188 72-73 20.409782423334626 27.780706352881047 30.12333700795282 21.686174215831507 74-75 20.69434071210248 28.264020280953584 29.49544320652266 21.54619580042128 76-77 18.859266997968376 28.538408547267363 30.45389901768428 22.14842543707999 78-79 19.55176901859171 28.386736241298195 30.01146309718557 22.050031642924523 80-81 20.2759326272353 28.63238343949738 29.128283839953113 21.96340009331421 82-83 20.659908949684787 28.56742677575796 28.832576157990893 21.940088116566358 84-85 20.562096018624548 28.26760010220788 28.726944079040763 22.443359800126814 86-87 20.50777502410287 28.29152939367233 28.80372869927307 22.39696688295173 88-89 20.73638463502179 28.078843024910586 28.61872340848378 22.566048931583843 90-91 19.9584613282888 28.147750925453334 29.302551613827436 22.591236132430428 92-93 19.13814991764137 28.450672110530384 29.98262571418053 22.428552257647713 94-95 18.794289861992205 28.354780909336025 29.666691368532444 23.184237860139323 96-97 18.83164029555172 28.510355437328833 29.046957465289353 23.611046801830096 98-99 18.843331573643038 28.30448241109565 29.133992417334547 23.718193597926764 100-101 19.25618186573108 28.197523551812793 29.1042967056719 23.441997876784225 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1402.0 1 1051.0 2 696.0 3 714.0 4 764.5 5 915.0 6 1175.0 7 1527.0 8 1985.0 9 2328.0 10 2534.5 11 2778.5 12 3009.5 13 3257.0 14 3603.5 15 3986.5 16 4344.5 17 4725.0 18 5124.0 19 5609.5 20 6349.0 21 7325.0 22 8379.5 23 9789.0 24 11817.0 25 14468.0 26 18001.0 27 22265.0 28 26507.5 29 30759.5 30 35355.5 31 40692.0 32 46872.0 33 53227.0 34 59207.5 35 64350.0 36 69036.5 37 72754.0 38 74686.5 39 75436.0 40 75870.0 41 76220.5 42 76506.5 43 77137.5 44 78039.5 45 79165.5 46 80248.5 47 81641.5 48 82843.0 49 78450.0 50 70321.5 51 63462.5 52 57288.0 53 51811.5 54 46960.0 55 42243.5 56 37422.5 57 32880.0 58 28471.0 59 23943.0 60 19489.5 61 15576.0 62 11871.5 63 8462.0 64 5930.0 65 4147.5 66 2770.0 67 1866.5 68 1334.5 69 873.5 70 581.0 71 447.5 72 331.0 73 244.0 74 159.5 75 99.5 76 67.5 77 34.0 78 12.0 79 5.5 80 2.5 81 0.5 82 0.5 83 1.0 84 0.5 85 1.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023895789751119435 2 0.008233400825138182 3 2.8391037328062696E-4 4 0.0 5 0.14365864887999724 6 0.0 7 4.7318395546771155E-5 8 0.0014195518664031348 9 0.0 10-11 0.0 12-13 0.0 14-15 3.7854716437416924E-4 16-17 0.0 18-19 1.4195518664031348E-4 20-21 0.0 22-23 1.6561438441369905E-4 24-25 0.0 26-27 2.8391037328062696E-4 28-29 0.002531534161752257 30-31 0.012373760435480658 32-33 0.0025078749639788713 34-35 0.003596198061554608 36-37 0.11105627434827191 38-39 0.11015722483288326 40-41 0.12295685082828486 42-43 0.12577229536331774 44-45 0.1215846173574285 46-47 0.11526761155193455 48-49 0.11429758444322573 50-51 0.11280705498350244 52-53 0.09132450340526833 54-55 0.07909269815642798 56-57 0.5518981064597653 58-59 1.55788719578412 60-61 0.5183966824126515 62-63 0.004897453939090815 64-65 0.11926601597563671 66-67 1.8858509953187914 68-69 2.8436226395809863 70-71 2.7536467104488005 72-73 2.6062972267161553 74-75 1.9892653487862595 76-77 1.397903700440487 78-79 0.9306582036138952 80-81 0.053209535792344165 82-83 2.8391037328062696E-4 84-85 1.4195518664031348E-4 86-87 2.6025117550724136E-4 88-89 2.1293277996047022E-4 90-91 0.24544051770110198 92-93 0.3889808705922323 94-95 1.9861659938779461 96-97 2.405075749653511 98-99 1.4026591991929376 100-101 0.9378742589347778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2113343.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.089091299625835 #Duplication Level Percentage of deduplicated Percentage of total 1 72.01446306391722 35.351245522381646 2 14.629477964691615 14.362955589492222 3 5.538740964479234 8.156752826708964 4 2.675001550156123 5.252535812890182 5 1.4803218428195486 3.6333827047499594 6 0.9201168619004555 2.7100620384094007 7 0.5768814859125514 1.9823011551717533 8 0.3883378387967652 1.5250521299035031 9 0.28676339721737093 1.2669259128654953 >10 1.3239133860701222 11.379580045387506 >50 0.09557592120058198 3.2572328614842703 >100 0.0638314901142123 5.826324454923841 >500 0.0038671957201108715 1.2872855363389213 >1k 0.002320317432066518 2.1878253922610176 >5k 1.9335978600554314E-4 0.6441648202598493 >10k+ 1.9335978600554314E-4 1.176373196771597 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13957 0.660422846646285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10830 0.5124582237715316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7909 0.37424119037941306 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5664 0.26801139237691185 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4292 0.2030905536867418 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3450 0.1632484646363605 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3251 0.15383210392255303 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2663 0.1260088873410516 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2638 0.12482592745238232 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2402 0.11365878610334432 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2180 0.10315410229196113 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2116 0.10012572497696778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.097759332015674E-5 0.0 14-15 0.0 0.0 0.0 9.463679109354231E-5 4.7318395546771155E-5 16-17 0.0 0.0 0.0 2.1293277996047022E-4 4.7318395546771155E-5 18-19 0.0 0.0 0.0 3.548879666007837E-4 4.7318395546771155E-5 20-21 0.0 0.0 0.0 5.678207465612539E-4 4.7318395546771155E-5 22-23 0.0 0.0 0.0 9.700271087088087E-4 4.7318395546771155E-5 24-25 0.0 0.0 0.0 0.0019873726129643887 4.7318395546771155E-5 26-27 0.0 0.0 0.0 0.0028154445350328837 4.7318395546771155E-5 28-29 0.0 0.0 0.0 0.006506279387681034 4.7318395546771155E-5 30-31 0.0 0.0 0.0 0.017720739132265798 4.7318395546771155E-5 32-33 0.0 0.0 0.0 0.0418294616633457 4.7318395546771155E-5 34-35 0.0 0.0 0.0 0.07410060742624364 4.7318395546771155E-5 36-37 0.0 0.0 0.0 0.12584327295663789 4.7318395546771155E-5 38-39 0.0 0.0 0.0 0.19729405023226235 4.7318395546771155E-5 40-41 0.0 0.0 0.0 0.28045613040571266 9.463679109354231E-5 42-43 0.0 0.0 0.0 0.36245890988826707 9.463679109354231E-5 44-45 0.0 0.0 0.0 0.46130703818547203 1.182959888669279E-4 46-47 0.0 0.0 0.0 0.5709437606673408 1.4195518664031348E-4 48-49 0.0 0.0 0.0 0.6753281412435179 1.4195518664031348E-4 50-51 0.0 0.0 0.0 0.779333974655321 1.4195518664031348E-4 52-53 0.0 0.0 0.0 0.878371376534713 1.4195518664031348E-4 54-55 0.0 0.0 0.0 0.98722734549006 1.4195518664031348E-4 56-57 0.0 0.0 2.3659197773385578E-5 1.0742695340983457 1.4195518664031348E-4 58-59 0.0 0.0 4.7318395546771155E-5 1.1351683091670401 1.4195518664031348E-4 60-61 0.0 0.0 4.7318395546771155E-5 1.1877153874217294 1.4195518664031348E-4 62-63 0.0 0.0 4.7318395546771155E-5 1.2322656568290147 1.4195518664031348E-4 64-65 0.0 0.0 4.7318395546771155E-5 1.2759641951164578 1.4195518664031348E-4 66-67 0.0 0.0 4.7318395546771155E-5 1.3150965082336374 1.4195518664031348E-4 68-69 0.0 0.0 7.097759332015674E-5 1.3672413801261794 1.4195518664031348E-4 70-71 0.0 0.0 9.463679109354231E-5 1.4518230121660327 1.4195518664031348E-4 72-73 0.0 0.0 9.463679109354231E-5 1.5700007050440936 1.4195518664031348E-4 74-75 0.0 0.0 9.463679109354231E-5 1.7226735082757507 1.4195518664031348E-4 76-77 0.0 2.3659197773385578E-5 9.463679109354231E-5 1.9616077465891717 1.4195518664031348E-4 78-79 0.0 4.7318395546771155E-5 9.463679109354231E-5 2.3285145856588354 1.4195518664031348E-4 80-81 4.7318395546771155E-5 4.7318395546771155E-5 9.463679109354231E-5 2.767274408366271 1.4195518664031348E-4 82-83 4.7318395546771155E-5 4.7318395546771155E-5 9.463679109354231E-5 3.2004506603991874 1.4195518664031348E-4 84-85 4.7318395546771155E-5 9.463679109354231E-5 9.463679109354231E-5 3.5406462651826986 1.4195518664031348E-4 86-87 4.7318395546771155E-5 9.463679109354231E-5 9.463679109354231E-5 3.8001166871634187 1.4195518664031348E-4 88-89 4.7318395546771155E-5 9.463679109354231E-5 9.463679109354231E-5 4.042363213165113 1.4195518664031348E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10895 0.0 25.190037 1 TACATGG 10895 0.0 24.70597 2 ACATGGG 10810 0.0 23.76728 3 CATGGGA 8385 0.0 22.223103 4 GTATCAA 21665 0.0 22.05438 1 GAGTACT 10510 0.0 21.999329 12-13 AGTACTT 10900 0.0 21.664894 12-13 GGTATCA 16345 0.0 20.21807 1 GTACTTT 11780 0.0 19.58746 14-15 ATCAACG 24710 0.0 19.19554 3 TCAACGC 24780 0.0 19.141315 4 AACGCAG 24985 0.0 19.015533 6 CAACGCA 25185 0.0 18.833504 5 CTTTGCG 550 0.0 18.823185 2 GTGTAGC 1415 0.0 18.630268 1 TATCAAC 25780 0.0 18.509222 2 ATGGGAG 3215 0.0 18.295387 5 ATGGGAT 3740 0.0 17.991898 5 CGCAGAG 26510 0.0 17.673904 8 AGAGTAC 20195 0.0 17.662207 10-11 >>END_MODULE