FastQCFastQC Report
Fri 27 May 2016
SRR939672_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939672_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007412
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.2712447668938912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.18526341378849984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.1345513526869422No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20970.10446286063847382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG1453.0651427E-642.7827031
GTACATG81150.036.8208961
TACATGG99400.030.127082
ACATGGG98300.028.7824333
GTGTAGC11500.026.9717061
GTACAGG10850.026.681691
CATGGGA76650.024.3380014
GAGTACT91750.023.55659312-13
GTATCAA194150.022.4196971
GTATAGG6557.2759576E-1222.0989551
CATGGGG27900.022.0058984
GGTATCA158000.021.3326851
AGTACTT98950.021.10330812-13
TCGCGCA1353.9694158E-420.972969
GTACTTT103850.020.90566414-15
CATTCCG3900.020.5696359
CGTTCGC1150.003867482320.518056
ATGGGAT31900.020.2666975
TAGCCCT14700.020.2405914
TATCAAC239950.019.2124712
Produced by FastQC (version 0.11.5)