FastQCFastQC Report
Fri 27 May 2016
SRR939672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007412
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134630.6706645172988903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101710.5066722725579004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74390.3705766429611858No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53890.2684551053794637No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38870.19363239833178242No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32110.15995719862190722No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30700.1529332294516522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25220.12563439891761133No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24360.12135027587759763No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21220.10570824524312897No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21210.10565842985894276No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21150.10535953755382553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG104500.026.0034141
TACATGG104150.025.6333622
ACATGGG104850.023.9752923
CGGGAGT6000.022.0095864
GAGTACT99200.021.85759712-13
CATGGGA79150.021.68984
GTATCAA215000.021.2842061
AGTACTT103800.021.1614212-13
GTCGGGA6250.021.131312
GTACTTT111650.019.42027514-15
ATGGGAG31350.018.9556265
CATGGGG28750.018.5372684
ATCAACG244550.018.514363
GCGCGAC650.007069179318.48575892-93
TCAACGC245900.018.4318964
CAACGCA249000.018.2024215
AACGCAG249950.018.1794246
GGTATCA164300.017.8311041
TATCAAC256200.017.7846952
TACTTTT125400.017.42241914-15
Produced by FastQC (version 0.11.5)