FastQCFastQC Report
Fri 27 May 2016
SRR939671_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939671_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4197427
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74160.17667966590008594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51290.12219390593332534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46450.11066303237673937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA168550.025.083351
TACATGG118750.024.4151692
GTACATG121800.024.3483371
ACATGGG120800.022.6259943
ATCAACG192850.021.775883
TCAACGC193400.021.5907614
TATCAAC196900.021.5207732
CAACGCA193150.021.4963875
AACGCAG198650.020.7790576
GAGTACT78850.019.13716112-13
AGAGTAC139350.018.89885710-11
GGTATCA124750.018.7898371
ACGCAGA216700.018.6543887
CGCAGAG216750.018.5421858
CATGGGA65700.017.6224484
AGTACTT90050.017.57374212-13
CATGGGG67550.017.139824
AGTACAT86600.016.87662
GTACTTT90150.016.52783814-15
GCAGAGT224600.016.5214869
Produced by FastQC (version 0.11.5)