FastQCFastQC Report
Fri 27 May 2016
SRR939671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4197427
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75610.18013416314327801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59380.1414676181384453No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49880.11883470516580753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149800.025.5191041
TACATGG111100.024.2746492
GTACATG117000.024.2634541
ACATGGG111650.022.931193
GGTATCA107850.022.6552261
ATCAACG168550.022.1706373
TCAACGC168650.022.1574924
CAACGCA170700.021.8913965
AACGCAG175800.021.3048636
TATCAAC179050.020.9505962
ACGCAGA194300.018.9861167
CGCAGAG190850.018.8368638
AGAGTAC136450.018.37363210-11
CATGGGA59950.017.6636224
GAGTACT81900.017.2709712-13
AGTACTT90550.017.10019512-13
GCAGAGT205700.016.3801929
CATGGGG60850.016.3195554
GTACTTT87850.016.07447614-15
TACTTTT106700.014.15959214-15
Produced by FastQC (version 0.11.5)