##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939671_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4197427 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.586558622699098 31.0 31.0 34.0 30.0 34.0 2 31.804756580638568 31.0 31.0 34.0 30.0 34.0 3 31.85816525218902 31.0 31.0 34.0 30.0 34.0 4 35.63318647352295 37.0 35.0 37.0 33.0 37.0 5 35.36138377153432 37.0 35.0 37.0 33.0 37.0 6 35.37735236372187 37.0 35.0 37.0 33.0 37.0 7 35.3071152875321 37.0 35.0 37.0 32.0 37.0 8 35.27214624578343 37.0 35.0 37.0 32.0 37.0 9 36.9009304986126 39.0 37.0 39.0 33.0 39.0 10-11 36.78104538804367 39.0 36.0 39.0 32.0 39.0 12-13 36.728147505602834 39.0 35.0 39.0 32.0 39.0 14-15 37.82826145636363 40.0 37.0 41.0 32.0 41.0 16-17 37.78810030525844 40.0 37.0 41.0 32.0 41.0 18-19 37.75139436612001 40.0 37.0 41.0 32.0 41.0 20-21 37.67781774406083 39.5 37.0 41.0 32.0 41.0 22-23 37.544499642280854 39.0 36.0 41.0 32.0 41.0 24-25 37.427713811342045 39.0 36.0 41.0 32.0 41.0 26-27 37.18257482500589 39.0 36.0 40.5 31.0 41.0 28-29 36.99463147304289 39.0 36.0 40.0 31.0 41.0 30-31 36.78512276687599 39.0 36.0 40.0 30.0 41.0 32-33 36.649470020562596 38.5 35.0 40.0 30.0 41.0 34-35 36.655550412192994 39.0 35.0 40.0 30.0 41.0 36-37 36.58422004718605 38.5 35.0 40.0 30.0 41.0 38-39 36.6495691288973 39.0 35.0 40.0 30.0 41.0 40-41 36.57619251031644 39.0 35.0 40.0 30.0 41.0 42-43 36.42232908398407 39.0 35.0 40.0 30.0 41.0 44-45 36.257195658197276 39.0 35.0 40.0 30.0 41.0 46-47 36.023520361402355 38.0 35.0 40.0 29.0 41.0 48-49 35.78352023751694 38.0 35.0 40.0 28.5 41.0 50-51 35.56250007445037 38.0 34.0 40.0 28.0 41.0 52-53 35.3501996580286 38.0 34.0 40.0 27.0 41.0 54-55 35.0742458415596 38.0 34.0 40.0 27.0 41.0 56-57 33.07796121290495 37.0 31.0 40.0 17.0 41.0 58-59 28.073760901619018 33.0 18.5 38.5 7.5 40.0 60-61 28.82871447198486 33.5 22.0 38.0 9.5 40.0 62-63 31.907616380225313 35.5 30.0 38.5 19.0 40.0 64-65 32.16719945337942 36.0 31.0 39.0 13.5 40.0 66-67 25.40924868973302 28.5 18.5 36.0 4.0 39.0 68-69 17.80929447968958 13.0 8.0 29.0 2.0 35.5 70-71 16.649556502114272 13.0 7.5 27.0 2.0 34.0 72-73 17.817323088644542 15.5 7.5 29.0 2.0 34.5 74-75 18.849585114881094 17.5 9.0 30.0 2.0 34.5 76-77 20.076521759639895 22.5 9.0 30.0 2.0 34.5 78-79 23.236150384509365 27.5 11.5 33.5 2.0 36.0 80-81 26.912061365212544 31.5 24.0 35.0 2.0 36.0 82-83 28.215939907948368 33.5 28.5 35.0 2.0 36.0 84-85 28.267121977344694 34.0 29.0 35.0 2.0 36.0 86-87 28.03054764263917 34.0 29.0 35.0 2.0 36.0 88-89 27.61844756323338 34.0 27.5 35.0 2.0 35.5 90-91 26.310964193063988 33.0 23.0 35.0 2.0 35.0 92-93 23.8758625224453 31.5 7.0 34.5 2.0 35.0 94-95 16.365002178715677 10.5 2.0 32.5 2.0 35.0 96-97 13.731817372881054 2.0 2.0 30.0 2.0 34.0 98-99 13.42516653654727 2.0 2.0 30.5 2.0 34.0 100-101 13.126853188870228 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4426520965026839 1101 2 0.36894099064568664 1101 3 0.4679077348265892 1101 4 0.30294090215333824 1101 5 0.4432598947927602 1101 6 0.5090342611681464 1101 7 0.37990115820390713 1101 8 0.39816344545192095 1101 9 0.43415575320422306 1101 10-11 0.4428559020082119 1101 12-13 0.5312743319529503 1101 14-15 0.6446461762099673 1101 16-17 0.6806566174495359 1101 18-19 0.5377463282625783 1101 20-21 0.5255683302539964 1101 22-23 0.5912362456553666 1101 24-25 0.5571161468102801 1101 26-27 0.47800799892947055 1101 28-29 0.4773113475642887 1101 30-31 0.38543638860787865 1101 32-33 0.5594762394279016 1101 34-35 0.553392164829198 1101 36-37 0.2985204726497628 1101 38-39 0.364709082880303 1101 40-41 0.29911221646379005 1101 42-43 0.22972550524629298 1101 44-45 0.2541702603513727 1101 46-47 0.32841713835885145 1101 48-49 0.35068913480318287 1101 50-51 0.17343175355436102 1101 52-53 -0.013589342990002251 1101 54-55 0.1146572025147492 1101 56-57 -2.3481505601974533 1101 58-59 -10.347762088173877 1101 60-61 -10.625646614706618 1101 62-63 -4.01859583007737 1101 64-65 -5.0232072723924865 1101 66-67 -9.705182580828282 1101 68-69 -4.902687700492999 1101 70-71 -4.770385796564955 1101 72-73 -4.914231357962944 1101 74-75 -6.632561889632939 1101 76-77 -8.048080925785406 1101 78-79 -11.393939942241715 1101 80-81 -7.992827967065313 1101 82-83 -3.9760552281255173 1101 84-85 -3.1226954556831537 1101 86-87 -2.962345375928031 1101 88-89 -2.959579646197888 1101 90-91 -9.81240505267671 1101 92-93 -17.513771328367373 1101 94-95 -11.698266762721813 1101 96-97 -9.622854212475922 1101 98-99 -9.773880526353508 1101 100-101 -9.6400162012329 1102 1 0.2922123530425118 1102 2 0.3141299213647777 1102 3 0.40049338847255456 1102 4 0.29019707982780574 1102 5 0.16901557555816993 1102 6 0.23063043412618356 1102 7 0.31656525610341646 1102 8 0.3565736312453751 1102 9 0.3629129962853952 1102 10-11 0.440022362331284 1102 12-13 0.35272606439690435 1102 14-15 0.3419485495823764 1102 16-17 0.44650112070892334 1102 18-19 0.4613702481170421 1102 20-21 0.47755054469973146 1102 22-23 0.5890675323971664 1102 24-25 0.4485023019975074 1102 26-27 0.4726446447091064 1102 28-29 0.500355241899527 1102 30-31 0.4115967554783637 1102 32-33 0.48296382512365454 1102 34-35 0.2714562829419549 1102 36-37 0.32282137845601966 1102 38-39 0.1886479925439133 1102 40-41 0.17319867232310315 1102 42-43 0.2864705193113437 1102 44-45 0.338569000392134 1102 46-47 0.3530017666034908 1102 48-49 0.31382416773393373 1102 50-51 0.38881893203735274 1102 52-53 0.22035902057898227 1102 54-55 1.0466280413368168E-4 1102 56-57 -3.235431162215413 1102 58-59 -10.576548966827001 1102 60-61 -10.819265597053715 1102 62-63 -4.810677552667361 1102 64-65 -5.935167252263458 1102 66-67 -7.14323897158409 1102 68-69 -4.655394644165408 1102 70-71 -4.587198746099208 1102 72-73 -4.99529122708215 1102 74-75 -5.77785462381186 1102 76-77 -8.066797394951777 1102 78-79 -10.491644422424308 1102 80-81 -6.443413414367686 1102 82-83 -3.969111660992265 1102 84-85 -3.4938338827554354 1102 86-87 -3.400350707172663 1102 88-89 -3.6255375331552884 1102 90-91 -9.557254885075182 1102 92-93 -13.981150354172954 1102 94-95 -10.734497960188419 1102 96-97 -9.499394156805263 1102 98-99 -9.6628029285298 1102 100-101 -9.534334593605768 1103 1 0.2376148130438871 1103 2 0.2263943483017279 1103 3 0.23943492550763068 1103 4 0.2681396926734152 1103 5 0.25116000273190764 1103 6 0.30101198078218516 1103 7 0.21411689142576762 1103 8 0.21450292809274885 1103 9 0.3658718646810186 1103 10-11 0.39471194441643576 1103 12-13 0.33563931599604757 1103 14-15 0.5397280040626313 1103 16-17 0.39204697538009725 1103 18-19 0.3608174815268015 1103 20-21 0.30119983077965884 1103 22-23 0.3834869327535273 1103 24-25 0.4297395261633312 1103 26-27 0.38377289671484505 1103 28-29 0.3990076513505798 1103 30-31 0.39554885901041104 1103 32-33 0.3791782915194517 1103 34-35 0.3658800762691854 1103 36-37 0.24687322112036725 1103 38-39 0.34970415776698616 1103 40-41 0.38154454587471065 1103 42-43 0.25617927157365017 1103 44-45 0.3336759396348583 1103 46-47 0.2656225771969716 1103 48-49 0.2328079318120828 1103 50-51 0.2843806060770504 1103 52-53 0.38227804628658646 1103 54-55 0.3176568324488329 1103 56-57 -2.657084434994289 1103 58-59 -10.494269634944906 1103 60-61 -10.772016702851715 1103 62-63 -4.405435258514654 1103 64-65 -5.371764376020302 1103 66-67 -6.578070494919956 1103 68-69 -4.1220001597428535 1103 70-71 -4.001229279739238 1103 72-73 -4.37408261798085 1103 74-75 -5.113850261062133 1103 76-77 -7.004774293221477 1103 78-79 -9.473771378970973 1103 80-81 -4.967247111756802 1103 82-83 -2.6286453190938808 1103 84-85 -2.124644443519724 1103 86-87 -1.9590149776450758 1103 88-89 -1.9771943841802972 1103 90-91 -5.135974859041376 1103 92-93 -10.45048971522313 1103 94-95 -8.8604505883845 1103 96-97 -8.450081929360286 1103 98-99 -8.571813714052189 1103 100-101 -8.541222639521973 1104 1 0.20689316315099404 1104 2 0.18930327706363315 1104 3 0.15437366877485204 1104 4 0.14515118009683192 1104 5 0.22683204996386053 1104 6 0.16162707471502102 1104 7 0.220859390195713 1104 8 0.24828830586830009 1104 9 0.239750475031272 1104 10-11 0.2683705449035969 1104 12-13 0.2926742982999144 1104 14-15 0.32858641147529255 1104 16-17 0.29073947849308723 1104 18-19 0.34455944300413677 1104 20-21 0.28145431338158744 1104 22-23 0.3887696309979418 1104 24-25 0.40644103055433334 1104 26-27 0.31722879727129794 1104 28-29 0.40848691890108313 1104 30-31 0.43885427519714426 1104 32-33 0.37541305585057927 1104 34-35 0.3747318234038346 1104 36-37 0.3312723828420445 1104 38-39 0.308401719748737 1104 40-41 0.25623169813052726 1104 42-43 0.36607068980260493 1104 44-45 0.32910295427699054 1104 46-47 0.2988972582095428 1104 48-49 0.2002965797728251 1104 50-51 0.1597452449804848 1104 52-53 0.17384985209054094 1104 54-55 0.1515321833937051 1104 56-57 -2.7668186265245325 1104 58-59 -10.342894587447276 1104 60-61 -10.679976527285206 1104 62-63 -4.359577150729624 1104 64-65 -5.594771364219387 1104 66-67 -6.856303552024343 1104 68-69 -4.103441213028756 1104 70-71 -4.205188438710795 1104 72-73 -4.3444440413337535 1104 74-75 -5.168940153595125 1104 76-77 -6.663586132445648 1104 78-79 -9.376825317320112 1104 80-81 -5.208680746500249 1104 82-83 -2.867249891824599 1104 84-85 -2.349427439835633 1104 86-87 -2.1391907478705754 1104 88-89 -2.05927509577187 1104 90-91 -4.698192380778526 1104 92-93 -7.129792038369974 1104 94-95 -8.02960481512877 1104 96-97 -8.056374788631214 1104 98-99 -8.472613852803265 1104 100-101 -8.719184588793514 1105 1 0.28516549753118525 1105 2 0.26271060190691387 1105 3 0.32282253479172596 1105 4 0.23955839539834756 1105 5 0.22080826923711072 1105 6 0.26740173262286504 1105 7 0.27071546135042723 1105 8 0.23809742087247088 1105 9 0.2978765542152857 1105 10-11 0.3659988245219381 1105 12-13 0.37079973943137645 1105 14-15 0.5060272448058001 1105 16-17 0.43353157386895447 1105 18-19 0.4852616904172038 1105 20-21 0.44881611853953274 1105 22-23 0.5010135488890484 1105 24-25 0.47059371370647085 1105 26-27 0.4994414731733485 1105 28-29 0.5212691724575222 1105 30-31 0.5820447299309919 1105 32-33 0.5231027974336513 1105 34-35 0.5077687446252384 1105 36-37 0.42428994227410044 1105 38-39 0.34242578731680595 1105 40-41 0.418924765491937 1105 42-43 0.42590076455074666 1105 44-45 0.35052692382213024 1105 46-47 0.5071288419237376 1105 48-49 0.4395444908565267 1105 50-51 0.47001803220859273 1105 52-53 0.6047264854768315 1105 54-55 0.44544606549740706 1105 56-57 1.8534477575921429 1105 58-59 -8.828105884478244 1105 60-61 -9.94180602157492 1105 62-63 -3.704345797391934 1105 64-65 -5.087514504589819 1105 66-67 -6.53516656496495 1105 68-69 -4.091461070579289 1105 70-71 -4.014144240302425 1105 72-73 -2.313176163157747 1105 74-75 -4.263099347141873 1105 76-77 -6.285855211063886 1105 78-79 -9.181818276156124 1105 80-81 -4.518788374107938 1105 82-83 -2.1219058121052967 1105 84-85 -1.54762790578339 1105 86-87 -1.4435784884530918 1105 88-89 -1.4770186878609195 1105 90-91 -4.132733214447825 1105 92-93 -6.556162976149121 1105 94-95 -8.038919817720506 1105 96-97 -8.390838011395122 1105 98-99 -8.695792297468298 1105 100-101 -8.785335646887917 1106 1 0.1801187208988928 1106 2 0.20449126593468137 1106 3 0.12691447473371653 1106 4 0.04986965625824524 1106 5 -0.04241249219218446 1106 6 0.11704376883417922 1106 7 0.12925037375773485 1106 8 0.09439739223787313 1106 9 0.1270443068324667 1106 10-11 0.27845768777559954 1106 12-13 0.17866439690925517 1106 14-15 0.23431317424301312 1106 16-17 0.21220247080291443 1106 18-19 0.18893688410843623 1106 20-21 0.22769644318620408 1106 22-23 0.2538001292693082 1106 24-25 0.40734285929472236 1106 26-27 0.27954409980928574 1106 28-29 0.26957708906028444 1106 30-31 0.28361212111874323 1106 32-33 0.2451303587792708 1106 34-35 0.34181021962359637 1106 36-37 0.34964601643048354 1106 38-39 0.32317451138376185 1106 40-41 0.2088623401951466 1106 42-43 0.28682500331120053 1106 44-45 0.1723031170269138 1106 46-47 0.23889212603881305 1106 48-49 0.08048189274854423 1106 50-51 0.14098831683896407 1106 52-53 0.13431503019651814 1106 54-55 0.12048937022437656 1106 56-57 1.5552341030620838 1106 58-59 -9.065239527115374 1106 60-61 -10.188972043571177 1106 62-63 -4.023231441235918 1106 64-65 -5.72184807283346 1106 66-67 -7.281690111978847 1106 68-69 -4.357105109016761 1106 70-71 -4.449867221461616 1106 72-73 -5.015270887210274 1106 74-75 -5.4250337394220125 1106 76-77 -7.1283498503103395 1106 78-79 -9.727028003387309 1106 80-81 -5.359267536246545 1106 82-83 -3.0581022598474767 1106 84-85 -2.5060704222278964 1106 86-87 -2.4172362693002754 1106 88-89 -2.3051921880966155 1106 90-91 -4.952069076908934 1106 92-93 -7.290349341252426 1106 94-95 -8.00891026135599 1106 96-97 -7.802412903090181 1106 98-99 -7.191177399826779 1106 100-101 -7.78179799647397 1107 1 0.15166352192846588 1107 2 0.04454025441537013 1107 3 0.11137172470061074 1107 4 0.16444927555617994 1107 5 0.060261160539702985 1107 6 0.0029938771000814768 1107 7 0.0925284818606471 1107 8 0.12218024468498356 1107 9 0.2274290384541473 1107 10-11 0.2008871225178055 1107 12-13 0.10449891590417337 1107 14-15 0.21001826087270814 1107 16-17 0.19739579306630617 1107 18-19 0.07922509891522367 1107 20-21 0.08845053527504376 1107 22-23 0.15272233741828245 1107 24-25 0.17196203837546165 1107 26-27 0.1638682387473267 1107 28-29 0.19139292270771335 1107 30-31 0.20868362868038304 1107 32-33 0.16961588352082657 1107 34-35 0.15277071303992784 1107 36-37 0.12648107534003827 1107 38-39 0.15097694564037312 1107 40-41 0.0258466736156322 1107 42-43 0.0960338868877173 1107 44-45 0.16795753996909468 1107 46-47 0.22972693366734376 1107 48-49 -0.009051686659091729 1107 50-51 0.03022058539725947 1107 52-53 -0.02008153879880581 1107 54-55 -0.06661821540643587 1107 56-57 1.2245952493563124 1107 58-59 -8.649070385557 1107 60-61 -10.25503857871147 1107 62-63 -3.824092701285746 1107 64-65 -5.4484102880907415 1107 66-67 -7.056074501767782 1107 68-69 -4.137369587953867 1107 70-71 -3.9974504526412886 1107 72-73 -2.418296446565229 1107 74-75 -4.715932951961616 1107 76-77 -6.842687130566784 1107 78-79 -7.699879918499597 1107 80-81 -2.4840146422701572 1107 82-83 -1.3771353259400456 1107 84-85 -1.1503823593216147 1107 86-87 -1.0250670688832884 1107 88-89 -0.8723737544829113 1107 90-91 -0.4268334443542372 1107 92-93 -3.2459514932140614 1107 94-95 -5.75913477302754 1107 96-97 -6.380775569521413 1107 98-99 -6.04419735629404 1107 100-101 -7.0188104462036165 1108 1 -0.08846144417633184 1108 2 -0.08754959819168207 1108 3 -0.10592436910431147 1108 4 -0.09965595678142591 1108 5 -0.12472421141136891 1108 6 -0.20366758264555784 1108 7 -0.17972504111585863 1108 8 -0.05659313141617872 1108 9 -0.09270652690236858 1108 10-11 -0.07657378501608747 1108 12-13 -0.09435957867761147 1108 14-15 -0.18239408092070875 1108 16-17 -0.2040979134348575 1108 18-19 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86-87 1.5158290920327957 2316 88-89 1.605690377069699 2316 90-91 2.7526930800750975 2316 92-93 4.738567295340257 2316 94-95 11.958800401747899 2316 96-97 13.921596674905066 2316 98-99 13.575677851924885 2316 100-101 12.431127609978375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 27.0 4 54.0 5 121.0 6 283.0 7 690.0 8 1724.0 9 3040.0 10 4769.0 11 7784.0 12 11498.0 13 16152.0 14 20380.0 15 28011.0 16 38731.0 17 51772.0 18 60357.0 19 64222.0 20 67548.0 21 68473.0 22 69174.0 23 59579.0 24 60064.0 25 71477.0 26 101536.0 27 151421.0 28 214361.0 29 290843.0 30 366343.0 31 408290.0 32 414669.0 33 383146.0 34 344859.0 35 312150.0 36 258618.0 37 171629.0 38 68621.0 39 5005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83925044609496 19.697975605850328 14.16296668395126 24.299807264103464 2 15.466499313934806 21.172074299466566 39.473301116026384 23.888125270572246 3 18.856753229488444 25.01118545847515 30.182679144825837 25.949382167210572 4 12.779614749702613 17.393655684780224 37.85983651413115 31.966893051386002 5 13.386423441850967 37.80662472361991 35.142305175152266 13.664646659376858 6 31.527242760862784 37.38261558807336 17.689432121154223 13.400709529909632 7 27.708141201740972 32.52182825335616 21.941441745145298 17.828588799757565 8 25.38537831675033 35.84909975749094 20.324041083831325 18.441480841927408 9 25.802759642990814 16.559740050273657 20.356232520541752 37.28126778619378 10-11 24.341007002623275 26.534445983217815 28.65805647126204 20.466490542896874 12-13 25.36025045819737 23.89145779068939 28.46493816330814 22.2833535878051 14-15 22.371833250226388 25.572705313261558 26.204512647463208 25.85094878904885 16-17 22.17936130872556 28.479566172324137 26.91095044654737 22.430122072402927 18-19 22.02354993977849 27.743146058394675 28.391925469463924 21.841378532362913 20-21 22.92100613066052 26.93993248721181 28.202503581360677 21.93655780076699 22-23 22.473273982419624 26.884675018487602 28.07761361297258 22.564437386120197 24-25 22.492013259079883 26.999752586436614 28.165371845194155 22.342862309289348 26-27 22.410233441296608 27.097844744920387 28.17132687618451 22.320594937598496 28-29 22.325890011972053 27.40296978396698 28.072213519485857 22.19892668457511 30-31 22.530471343808063 27.071865527220844 27.980680474655784 22.41698265431531 32-33 22.322708838246513 27.07885252355831 28.078352797246325 22.52008584094885 34-35 22.613851020977293 27.14156496364208 27.98543133879892 22.2591526765817 36-37 22.708330774403514 26.90815173080448 28.037733909538744 22.345783585253265 38-39 22.43594872869345 27.176542376745864 28.069057208168672 22.31845168639201 40-41 22.61801342536737 27.111542371579556 28.077668388369137 22.192775814683944 42-43 22.354496975872816 27.10936329695221 27.85040326466432 22.68573646251065 44-45 22.535288031795154 27.0102356312072 27.941370972310427 22.51310536468722 46-47 22.704574983349225 27.139638148970334 27.588246132160354 22.567540735520087 48-49 22.59111133848673 27.168254102271565 27.701076269772585 22.53955828946912 50-51 22.434656450425383 27.273056841953625 27.741013704203944 22.551273003417048 52-53 22.643197269057172 27.315604190221247 27.389333542217265 22.65186499850431 54-55 22.586338851231336 27.124847707445703 27.486487388626756 22.802326052696202 56-57 21.644173001805385 27.23921613153039 28.481859070680244 22.63475179598398 58-59 20.99290992977694 26.860477892417574 29.725305761698518 22.42130641610697 60-61 22.266054407653055 27.154304286152342 28.186189257577077 22.393452048617526 62-63 22.533445677629448 27.125834466723365 27.72923436282117 22.61148549282602 64-65 22.089041790233782 27.236629407896352 27.96762038655268 22.706708415317177 66-67 21.624013747617006 27.029598316368563 28.66890257272462 22.67748536328981 68-69 21.605669984276744 26.628933633739376 29.210556257963393 22.554840124020483 70-71 21.56609187305817 26.50985918736707 29.15257368389202 22.771475255682745 72-73 20.697659514251544 26.71711900174238 29.639589020085943 22.94563246392013 74-75 21.363258813357152 26.953521052466538 28.769694793746282 22.913525340430027 76-77 19.858949451007692 27.252603142700554 29.443136385144197 23.445311021147557 78-79 20.83507720046174 27.122100436406633 28.78404538619348 23.25877697693815 80-81 21.997384949767365 27.126206147525206 27.600185491941577 23.27622341076585 82-83 22.39401717271839 27.023022630484228 27.43670596189773 23.14625423489966 84-85 22.346136995139158 27.005809998954117 27.277929603882768 23.370123402023957 86-87 22.11125168720095 26.91465368945925 27.54299347180839 23.431101151531404 88-89 22.271180920735627 26.893835027768876 27.298536444628844 23.536447606866652 90-91 21.62592432271065 26.922964400328382 27.866390029106654 23.58472124785432 92-93 20.704761057472815 27.095989073523885 28.489014333227384 23.71023553577591 94-95 19.559055952606087 27.183366043210093 29.126779982041572 24.130798022142244 96-97 19.397206194959004 27.31234431801649 28.764672309869027 24.52577717715548 98-99 19.921893640095355 27.104805968140617 28.481966037130903 24.491334354633125 100-101 20.440308627969976 26.948099535177544 28.116341841631883 24.495249995220597 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 259.0 1 192.5 2 136.0 3 151.5 4 183.0 5 234.5 6 312.5 7 434.5 8 607.5 9 819.0 10 962.0 11 1099.0 12 1299.0 13 1546.0 14 1842.0 15 2135.0 16 2454.5 17 2813.5 18 3211.5 19 3698.5 20 4434.5 21 5476.5 22 6813.0 23 8685.0 24 11309.0 25 14949.5 26 19735.5 27 25435.5 28 32275.0 29 39978.5 30 48666.5 31 58653.0 32 68991.0 33 79615.5 34 90128.5 35 100827.5 36 111183.0 37 119865.0 38 127134.0 39 134036.0 40 140848.0 41 146258.0 42 151654.5 43 157107.0 44 162077.5 45 166556.0 46 170342.0 47 173242.5 48 174108.0 49 171217.5 50 164644.5 51 157792.0 52 151485.5 53 143435.0 54 133631.5 55 122474.0 56 110143.0 57 97272.0 58 83600.5 59 69911.0 60 56537.0 61 44332.5 62 33781.5 63 24949.0 64 18010.5 65 12721.0 66 8985.0 67 6298.0 68 4244.0 69 2819.5 70 1823.5 71 1110.0 72 663.0 73 400.5 74 236.0 75 125.5 76 63.5 77 29.0 78 16.0 79 10.0 80 7.0 81 5.0 82 2.5 83 1.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02408618422667029 2 0.007242532151244084 3 3.0971354594135883E-4 4 0.0 5 0.14742364786808682 6 0.0 7 0.0 8 0.0013817988972768319 9 0.0 10-11 0.0 12-13 0.0 14-15 5.717788540455856E-4 16-17 0.0 18-19 1.5485677297067942E-4 20-21 0.0 22-23 4.526582594527553E-4 24-25 3.57361783778491E-5 26-27 2.858894270227928E-4 28-29 0.003621266075622042 30-31 0.012698255383595711 32-33 0.003085223399954305 34-35 0.004729087605335363 36-37 0.10943609025243321 38-39 0.10853077373352771 40-41 0.12066916232253712 42-43 0.12252744359818528 44-45 0.11827483837122124 46-47 0.11148496447942989 48-49 0.11098465798214001 50-51 0.11021037411728662 52-53 0.08839939324733938 54-55 0.07616570818265571 56-57 0.5407122029757754 58-59 1.5593124073390676 60-61 0.5128260717815939 62-63 0.005014977032358156 64-65 0.1199782628738987 66-67 1.8865366806855723 68-69 2.844385381806521 70-71 2.7613106791374813 72-73 2.605024459031688 74-75 1.991362803927263 76-77 1.389982482125359 78-79 0.9245068466944154 80-81 0.049947265312773756 82-83 3.216256054006419E-4 84-85 1.905929513485285E-4 86-87 3.9309796215634003E-4 88-89 2.7397736756350975E-4 90-91 0.24394706566665722 92-93 0.3817576815511026 94-95 1.9888612714408136 96-97 2.4046874430454657 98-99 1.395700270665815 100-101 0.9281281127700375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4197427.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.954581759233015 #Duplication Level Percentage of deduplicated Percentage of total 1 67.2708022446096 30.91401581759107 2 15.572574850050724 14.312623282968637 3 6.395737618992205 8.817403419677387 4 3.344273767370632 6.147388090715677 5 2.000118842229977 4.595731243171997 6 1.2154737310982762 3.351395217117344 7 0.8570495026316537 2.7569746008277423 8 0.6120217133853298 2.2500161492953614 9 0.44425060841300795 1.8373815815314127 >10 2.1378054725404105 16.746913454934287 >50 0.1026462085238866 3.2335111407258905 >100 0.04480685841974048 3.623328278077007 >500 0.0017121957847279611 0.505551730215936 >1k 6.226165282986145E-4 0.5859039123156637 >5k 1.037694213830996E-4 0.3218620808346166 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7561 0.18013416314327801 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5938 0.1414676181384453 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4988 0.11883470516580753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.14723567556982E-5 2 0.0 0.0 0.0 0.0 1.1912059459283032E-4 3 0.0 0.0 0.0 0.0 1.1912059459283032E-4 4 0.0 0.0 0.0 0.0 1.1912059459283032E-4 5 0.0 0.0 0.0 0.0 1.1912059459283032E-4 6 0.0 0.0 0.0 2.3824118918566066E-5 1.6676883242996244E-4 7 2.3824118918566066E-5 0.0 0.0 2.3824118918566066E-5 1.6676883242996244E-4 8 2.3824118918566066E-5 0.0 0.0 2.3824118918566066E-5 1.6676883242996244E-4 9 2.3824118918566066E-5 0.0 0.0 2.3824118918566066E-5 1.6676883242996244E-4 10-11 2.3824118918566066E-5 0.0 0.0 2.3824118918566066E-5 1.6676883242996244E-4 12-13 2.3824118918566066E-5 2.3824118918566066E-5 0.0 2.3824118918566066E-5 2.620653081042267E-4 14-15 4.764823783713213E-5 2.3824118918566066E-5 0.0 2.3824118918566066E-5 2.7397736756350975E-4 16-17 4.764823783713213E-5 2.3824118918566066E-5 0.0 2.3824118918566066E-5 3.335376648599249E-4 18-19 5.9560297296415166E-5 2.3824118918566066E-5 0.0 2.3824118918566066E-5 3.69273843237774E-4 20-21 7.14723567556982E-5 4.764823783713213E-5 0.0 5.9560297296415166E-5 5.479547351270195E-4 22-23 7.14723567556982E-5 4.764823783713213E-5 0.0 1.429447135113964E-4 5.836909135048685E-4 24-25 7.14723567556982E-5 4.764823783713213E-5 0.0 4.1692208107490614E-4 5.956029729641516E-4 26-27 7.14723567556982E-5 4.764823783713213E-5 0.0 8.934044594462274E-4 6.551632702605668E-4 28-29 1.0720853513354729E-4 4.764823783713213E-5 0.0 0.002525356605368003 6.670753297198498E-4 30-31 1.1912059459283032E-4 4.764823783713213E-5 0.0 0.008350353680957406 6.789873891791328E-4 32-33 1.1912059459283032E-4 9.529647567426426E-5 0.0 0.021810980869947233 6.908994486384158E-4 34-35 1.3103265405211336E-4 9.529647567426426E-5 0.0 0.042502228150721855 7.266356270162649E-4 36-37 1.429447135113964E-4 9.529647567426426E-5 0.0 0.07362843951782842 7.385476864755479E-4 38-39 1.429447135113964E-4 1.1912059459283032E-4 0.0 0.11754820274420497 7.623718053941141E-4 40-41 1.429447135113964E-4 1.1912059459283032E-4 0.0 0.1702471537920731 7.623718053941141E-4 42-43 1.429447135113964E-4 1.1912059459283032E-4 0.0 0.22474482581829297 7.623718053941141E-4 44-45 1.9059295134852853E-4 1.1912059459283032E-4 0.0 0.2927984215091769 7.742838648533972E-4 46-47 1.9059295134852853E-4 1.1912059459283032E-4 0.0 0.36901177792966977 7.981079837719631E-4 48-49 1.9059295134852853E-4 1.3103265405211336E-4 0.0 0.437327438928658 8.100200432312462E-4 50-51 1.9059295134852853E-4 1.429447135113964E-4 0.0 0.5052500019654897 8.100200432312462E-4 52-53 1.9059295134852853E-4 1.7868089188924547E-4 0.0 0.5706948566347908 8.100200432312462E-4 54-55 1.9059295134852853E-4 2.1441707026709458E-4 0.0 0.6470630698282542 8.100200432312462E-4 56-57 2.1441707026709458E-4 2.1441707026709458E-4 0.0 0.7121029144759397 8.100200432312462E-4 58-59 2.1441707026709458E-4 2.1441707026709458E-4 0.0 0.7557487003347527 8.100200432312462E-4 60-61 2.1441707026709458E-4 2.1441707026709458E-4 0.0 0.7905676501342369 8.338441621498123E-4 62-63 2.978014864820758E-4 2.1441707026709458E-4 0.0 0.8239452407391481 8.338441621498123E-4 64-65 3.0971354594135883E-4 2.1441707026709458E-4 0.0 0.8573943037008147 8.338441621498123E-4 66-67 3.0971354594135883E-4 2.1441707026709458E-4 0.0 0.886066630819309 8.338441621498123E-4 68-69 3.0971354594135883E-4 2.1441707026709458E-4 0.0 0.9256980526403438 8.338441621498123E-4 70-71 3.0971354594135883E-4 2.1441707026709458E-4 0.0 0.9865567644178207 8.338441621498123E-4 72-73 3.0971354594135883E-4 2.1441707026709458E-4 0.0 1.075408815924613 8.338441621498123E-4 74-75 3.0971354594135883E-4 2.1441707026709458E-4 0.0 1.1886805893229353 8.457562216090952E-4 76-77 3.0971354594135883E-4 2.1441707026709458E-4 0.0 1.3728767647418287 8.934044594462274E-4 78-79 3.0971354594135883E-4 2.1441707026709458E-4 0.0 1.6489983030080095 9.053165189055104E-4 80-81 3.0971354594135883E-4 2.1441707026709458E-4 0.0 1.9776162873112504 9.291406378240765E-4 82-83 3.0971354594135883E-4 2.1441707026709458E-4 0.0 2.308235497603651 0.0010006129945797748 84-85 3.0971354594135883E-4 2.1441707026709458E-4 0.0 2.5661315848971284 0.0010006129945797748 86-87 3.0971354594135883E-4 2.1441707026709458E-4 0.0 2.7673858294617153 0.001024437113498341 88-89 3.0971354594135883E-4 2.1441707026709458E-4 0.0 2.96535234561554 0.0010482612324169069 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14980 0.0 25.519104 1 TACATGG 11110 0.0 24.274649 2 GTACATG 11700 0.0 24.263454 1 ACATGGG 11165 0.0 22.93119 3 GGTATCA 10785 0.0 22.655226 1 ATCAACG 16855 0.0 22.170637 3 TCAACGC 16865 0.0 22.157492 4 CAACGCA 17070 0.0 21.891396 5 AACGCAG 17580 0.0 21.304863 6 TATCAAC 17905 0.0 20.950596 2 ACGCAGA 19430 0.0 18.986116 7 CGCAGAG 19085 0.0 18.836863 8 AGAGTAC 13645 0.0 18.373632 10-11 CATGGGA 5995 0.0 17.663622 4 GAGTACT 8190 0.0 17.27097 12-13 AGTACTT 9055 0.0 17.100195 12-13 GCAGAGT 20570 0.0 16.380192 9 CATGGGG 6085 0.0 16.319555 4 GTACTTT 8785 0.0 16.074476 14-15 TACTTTT 10670 0.0 14.159592 14-15 >>END_MODULE