FastQCFastQC Report
Fri 27 May 2016
SRR939670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4229060
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG101300.029.4669461
GTATCAA141600.028.772061
TACATGG115350.025.0661662
ACATGGG117450.024.0146053
TATCAAC175500.022.7475012
ATCAACG182400.021.7833793
TCAACGC185250.021.4964544
CAACGCA185200.021.4841735
AACGCAG190200.021.0186586
GGTATCA107850.020.6645031
ACGCAGA198800.019.492577
CATGGGG64700.018.7640384
AGAGTAC135050.018.73289310-11
CGCAGAG207900.018.4116738
CATGGGA66700.018.3430444
GAGTACT76000.018.28958312-13
GTATAGA16300.017.9416871
AGTACTT85550.017.43408412-13
GAGTACA93600.017.2384011
GTACTTT83550.016.55465514-15
Produced by FastQC (version 0.11.5)